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Protein

Tight junction protein ZO-3

Gene

TJP3

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Names & Taxonomyi

Protein namesi
Recommended name:
Tight junction protein ZO-3
Alternative name(s):
Tight junction protein 3
Zona occludens protein 3
Zonula occludens protein 3
Gene namesi
Name:TJP3
Synonyms:ZO3
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  • bicellular tight junction Source: UniProtKB-SubCell
  • cell surface Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 898898Tight junction protein ZO-3PRO_0000094545Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281PhosphoserineBy similarity
Modified residuei156 – 1561PhosphoserineBy similarity
Modified residuei161 – 1611PhosphoserineBy similarity
Modified residuei313 – 3131PhosphoserineBy similarity
Modified residuei319 – 3191PhosphothreonineBy similarity
Modified residuei321 – 3211PhosphoserineBy similarity
Modified residuei360 – 3601PhosphoserineBy similarity
Modified residuei580 – 5801PhosphoserineBy similarity
Modified residuei884 – 8841PhosphoserineBy similarity
Modified residuei885 – 8851PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO62683.

Interactioni

Subunit structurei

Interacts with UBN1 (By similarity). Interacts with occludin, claudins and ZO-1. Interacts with INADL. Interacts with FASLG (By similarity).By similarity

Protein-protein interaction databases

IntActiO62683. 1 interaction.
STRINGi9615.ENSCAFP00000028316.

Structurei

3D structure databases

ProteinModelPortaliO62683.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 9383PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini187 – 26478PDZ 2PROSITE-ProRule annotationAdd
BLAST
Domaini369 – 43567PDZ 3PROSITE-ProRule annotationAdd
BLAST
Domaini464 – 53875SH3PROSITE-ProRule annotationAdd
BLAST
Domaini569 – 750182Guanylate kinase-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MAGUK family.Curated
Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation
Contains 3 PDZ (DHR) domains.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG3580. Eukaryota.
ENOG410XQP3. LUCA.
HOGENOMiHOG000230923.
HOVERGENiHBG017627.
InParanoidiO62683.
KOiK06097.

Family and domain databases

Gene3Di2.30.42.10. 3 hits.
3.40.50.300. 1 hit.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR005417. ZO.
IPR005420. ZO-3.
[Graphical view]
PANTHERiPTHR13865:SF11. PTHR13865:SF11. 1 hit.
PfamiPF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 3 hits.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSiPR01597. ZONOCCLUDNS.
PR01600. ZONOCCLUDNS3.
SMARTiSM00072. GuKc. 1 hit.
SM00228. PDZ. 3 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 3 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O62683-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEELTIWEQH TATLCRDPRR GFGIAISGGR DRASGSVVVS DVVPGGPADG
60 70 80 90 100
RLQTGDHVVM VNGVSMESVT STFAIQILKT CTKLANITVK RPRKIQLPAT
110 120 130 140 150
KAGTSGRGRQ GLEEEADCGQ GYDGDTSSGS GRSWDKRSRR ARTGRRNQAG
160 170 180 190 200
SRGRRSPGGN SEANGLALVS GFKRLPRQDV HMRPVKSVLV RRTESEEFGV
210 220 230 240 250
TLGSQIFIKH ITDSGLAARN RGLQEGDLIL QINGVSSENL SLSDTRRLIE
260 270 280 290 300
KSEGKLTLLV LRDRGQFLVN IPPAVSDSDS DSSFLDDISA LGSELSQAVP
310 320 330 340 350
SHVPPPPPHA QRSLDSDGTD SPRDSPPLRR ENSLDSRTIS EPDAPRHSSY
360 370 380 390 400
DIYRVPSSQS AEDRGYSPDS RVVRFHKGTT IGLRLAGGND VGIFVSGVQE
410 420 430 440 450
GSPADGQGIQ EGDQILQVND VPFRNLTREE AVQFLVALPP GEEVELVTQR
460 470 480 490 500
NEDIFRKMVQ SRVGDSFYIR THFELEASPP SGLGFTRGDV FHVLDTLCPG
510 520 530 540 550
PGPSGARGTH WLAVRMGRDL REQERGIIPN QSRAEQLASL ESAQRAVGAG
560 570 580 590 600
PGASVGSSAR AEFWRLRGLR RGAKKSTQRS REDLSALTRQ GHYPPYERVV
610 620 630 640 650
LREASFKRPV VILGPVADIA MQKLTAEMPD QFGIADSVLR TDSPSKIIKL
660 670 680 690 700
DTVRVIAEKN KHALLDVTPS AVERLNYVQY YPIVVFCAPE SRAALKALRQ
710 720 730 740 750
WLAPASRRSA RRLYAQAQKL RKHSEHLFTA TIPLRGTSDT WYQELKAVVR
760 770 780 790 800
EQQTRPIWTA EDQLDNSSED NLELPHRGLA DSSADLSCDS RVNSDYETDG
810 820 830 840 850
EGYTDGEGYT DVDEGPPAPA LARSSEPVLE EEPRSPRDHG RASGARGAQV
860 870 880 890
DRHPYHSQGR QDSMRTYGQE ALKKKFTRAR DVESSDEDGY DWGPATDL
Length:898
Mass (Da):98,415
Last modified:August 1, 1998 - v1
Checksum:i8091D6132DB9F15D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023617 mRNA. Translation: AAC39177.1.
RefSeqiNP_001003202.1. NM_001003202.1.
UniGeneiCfa.3713.

Genome annotation databases

GeneIDi403852.
KEGGicfa:403852.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023617 mRNA. Translation: AAC39177.1.
RefSeqiNP_001003202.1. NM_001003202.1.
UniGeneiCfa.3713.

3D structure databases

ProteinModelPortaliO62683.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO62683. 1 interaction.
STRINGi9615.ENSCAFP00000028316.

Proteomic databases

PaxDbiO62683.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi403852.
KEGGicfa:403852.

Organism-specific databases

CTDi27134.

Phylogenomic databases

eggNOGiKOG3580. Eukaryota.
ENOG410XQP3. LUCA.
HOGENOMiHOG000230923.
HOVERGENiHBG017627.
InParanoidiO62683.
KOiK06097.

Family and domain databases

Gene3Di2.30.42.10. 3 hits.
3.40.50.300. 1 hit.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR005417. ZO.
IPR005420. ZO-3.
[Graphical view]
PANTHERiPTHR13865:SF11. PTHR13865:SF11. 1 hit.
PfamiPF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 3 hits.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSiPR01597. ZONOCCLUDNS.
PR01600. ZONOCCLUDNS3.
SMARTiSM00072. GuKc. 1 hit.
SM00228. PDZ. 3 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 3 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin."
    Haskins J., Gu L., Wittchen E.S., Hibbard J., Stevenson B.R.
    J. Cell Biol. 141:199-208(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Cocker spaniel.
    Tissue: Kidney.
  2. "The carboxyl terminus of zona occludens-3 binds and recruits a mammalian homologue of discs lost to tight junctions."
    Roh M.H., Liu C.-J., Laurinec S., Margolis B.
    J. Biol. Chem. 277:27501-27509(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH INADL.

Entry informationi

Entry nameiZO3_CANLF
AccessioniPrimary (citable) accession number: O62683
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: August 1, 1998
Last modified: June 8, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.