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Protein

Stromal cell-derived factor 1

Gene

CXCL12

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Plays a protective role after myocardial infarction. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei46 – 461Important for dimer formationBy similarity
Binding sitei62 – 621HeparinBy similarity
Binding sitei69 – 691HeparinBy similarity
Binding sitei85 – 851HeparinBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Keywords - Biological processi

Chemotaxis

Names & Taxonomyi

Protein namesi
Recommended name:
Stromal cell-derived factor 1
Short name:
SDF-1
Alternative name(s):
C-X-C motif chemokine 12
Gene namesi
Name:CXCL12
Synonyms:SDF1
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
Proteomesi
  • UP000011712 Componenti: Chromosome D2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 9372Stromal cell-derived factor 1PRO_0000005108Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 55By similarity
Disulfide bondi32 ↔ 71By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Monomer or homodimer; in equilibrium. Dimer formation is induced by non acidic pH and the presence of multivalent anions, and by binding to CXCR4 or heparin. Monomeric form is required for full chemotactic activity and resistance to ischemia/reperfusion injury, whereas the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization but with no chemotactic activity and instead acts as a selective antagonist that blocks chemotaxis induced by the monomeric form. Interacts with the N-terminus of ACKR3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000007102.

Structurei

3D structure databases

ProteinModelPortaliO62657.
SMRiO62657. Positions 22-89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni29 – 335Receptor and heparin bindingBy similarity
Regioni39 – 413Receptor bindingBy similarity
Regioni41 – 5111Heparin bindingBy similarityAdd
BLAST
Regioni48 – 503Receptor bindingBy similarity
Regioni60 – 7011Receptor bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi22 – 232Receptor activation motifBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J1S5. Eukaryota.
ENOG4112BS6. LUCA.
GeneTreeiENSGT00390000014056.
HOVERGENiHBG107437.
InParanoidiO62657.
KOiK10031.
OMAiSTPNCAL.
OrthoDBiEOG7C5MBS.

Family and domain databases

InterProiIPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Beta (identifier: O62657-1) [UniParc]FASTAAdd to basket

Also known as: SDF-1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDAKVVAVLA LVLAALCLSD GKPVSLSYRC PCRFFESHVA RANVKHLKIL
60 70 80 90
NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNKR FKM
Length:93
Mass (Da):10,581
Last modified:August 1, 1998 - v1
Checksum:i44FC763711E9BE37
GO
Isoform Alpha (identifier: O62657-2) [UniParc]FASTAAdd to basket

Also known as: SDF-1a

The sequence of this isoform differs from the canonical sequence as follows:
     90-93: Missing.

Show »
Length:89
Mass (Da):10,018
Checksum:i81E9BE373B2F85FD
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei90 – 934Missing in isoform Alpha. 1 PublicationVSP_001055

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011965 mRNA. Translation: BAA28601.1.
AB011966 mRNA. Translation: BAA28602.1.
RefSeqiNP_001009847.1. NM_001009847.1. [O62657-1]

Genome annotation databases

EnsembliENSFCAT00000007670; ENSFCAP00000007102; ENSFCAG00000007670. [O62657-2]
GeneIDi493806.
KEGGifca:493806.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011965 mRNA. Translation: BAA28601.1.
AB011966 mRNA. Translation: BAA28602.1.
RefSeqiNP_001009847.1. NM_001009847.1. [O62657-1]

3D structure databases

ProteinModelPortaliO62657.
SMRiO62657. Positions 22-89.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000007102.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSFCAT00000007670; ENSFCAP00000007102; ENSFCAG00000007670. [O62657-2]
GeneIDi493806.
KEGGifca:493806.

Organism-specific databases

CTDi6387.

Phylogenomic databases

eggNOGiENOG410J1S5. Eukaryota.
ENOG4112BS6. LUCA.
GeneTreeiENSGT00390000014056.
HOVERGENiHBG107437.
InParanoidiO62657.
KOiK10031.
OMAiSTPNCAL.
OrthoDBiEOG7C5MBS.

Family and domain databases

InterProiIPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and sequencing of feline stromal cell-derived factor-1 alpha and beta."
    Nishimura Y., Miyazawa T., Ikeda Y., Izumiya Y., Nakamura K., Cai J.S., Sato E., Kohmoto M., Mikami T.
    Eur. J. Immunogenet. 25:303-305(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
    Tissue: Thymus.

Entry informationi

Entry nameiSDF1_FELCA
AccessioniPrimary (citable) accession number: O62657
Secondary accession number(s): Q54AJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: May 11, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.