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O62653

- SUIS_SUNMU

UniProt

O62653 - SUIS_SUNMU

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Protein

Sucrase-isomaltase, intestinal

Gene
SI
Organism
Suncus murinus (Asian house shrew) (Musk shrew)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Plays an important role in the final stage of carbohydrate digestion. Isomaltase activity is specific for both alpha-1,4- and alpha-1,6-oligosaccharides By similarity.

Catalytic activityi

Hydrolysis of sucrose and maltose by an alpha-D-glucosidase-type action.
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei250 – 2501Substrate By similarity
Binding sitei374 – 3741Substrate By similarity
Active sitei491 – 4911Nucleophile; for isomaltase activity By similarity
Binding sitei574 – 5741Substrate By similarity
Active sitei590 – 5901For isomaltase activity By similarity
Binding sitei648 – 6481Substrate By similarity
Active sitei1380 – 13801Nucleophile; for sucrase activity By similarity
Active sitei1383 – 13831For sucrase activity By similarity
Active sitei1486 – 14861Proton donor; for sucrase activity By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. oligo-1,6-glucosidase activity Source: UniProtKB-EC
  3. sucrose alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKO62653.

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Sucrase-isomaltase, intestinal
Cleaved into the following 2 chains:
Gene namesi
Name:SI
OrganismiSuncus murinus (Asian house shrew) (Musk shrew)
Taxonomic identifieri9378 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaInsectivoraSoricidaeCrocidurinaeSuncus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1211Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei13 – 3220Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini33 – 18131781Lumenal Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 18131812Sucrase-isomaltase, intestinalPRO_0000018562Add
BLAST
Chaini2 – 991990Isomaltase By similarityPRO_0000018563Add
BLAST
Chaini992 – 1813822Sucrase By similarityPRO_0000018564Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei7 – 71Phosphoserine; by PKA By similarity
Disulfide bondi48 ↔ 79 By similarity
Disulfide bondi62 ↔ 78 By similarity
Disulfide bondi73 ↔ 91 By similarity
Glycosylationi127 – 1271N-linked (GlcNAc...) Reviewed prediction
Modified residuei377 – 3771Sulfotyrosine Reviewed prediction
Glycosylationi388 – 3881N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi506 ↔ 531 By similarity
Disulfide bondi621 ↔ 632 By similarity
Glycosylationi669 – 6691N-linked (GlcNAc...) Reviewed prediction
Glycosylationi791 – 7911N-linked (GlcNAc...) Reviewed prediction
Glycosylationi896 – 8961N-linked (GlcNAc...) Reviewed prediction
Glycosylationi911 – 9111N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1221 – 12211N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1289 – 12891N-linked (GlcNAc...) Reviewed prediction
Modified residuei1294 – 12941Sulfotyrosine Reviewed prediction
Glycosylationi1326 – 13261N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1340 – 13401N-linked (GlcNAc...) Reviewed prediction
Modified residuei1368 – 13681Sulfotyrosine Reviewed prediction
Modified residuei1371 – 13711Sulfotyrosine Reviewed prediction
Glycosylationi1432 – 14321N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1521 – 15211N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1545 – 15451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1558 – 15581N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1703 – 17031N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1772 – 17721N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

The precursor is proteolytically cleaved when exposed to pancreatic proteases in the intestinal lumen By similarity.
Sulfated By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation

Interactioni

Subunit structurei

The resulting sucrase and isomaltase subunits stay associated with one another in a complex by non-covalent linkages By similarity.

Structurei

3D structure databases

ProteinModelPortaliO62653.
SMRiO62653. Positions 56-917.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 9550P-type 1Add
BLAST
Domaini917 – 96246P-type 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni95 – 991897IsomaltaseAdd
BLAST
Regioni992 – 1813822SucraseAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi42 – 454Ser/Thr-rich

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG080721.

Family and domain databases

Gene3Di4.10.110.10. 2 hits.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
IPR000519. P_trefoil.
IPR017957. P_trefoil_CS.
[Graphical view]
PfamiPF01055. Glyco_hydro_31. 2 hits.
PF00088. Trefoil. 2 hits.
[Graphical view]
SMARTiSM00018. PD. 2 hits.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 4 hits.
SSF74650. SSF74650. 2 hits.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 2 hits.
PS00707. GLYCOSYL_HYDROL_F31_2. 2 hits.
PS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O62653-1 [UniParc]FASTAAdd to Basket

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MARKKSSGLK ITLIVLLAIV TIIAIALVAI LPTKTPAVEL VSTIPGKCPS     50
AENDRLDEKI NCIPDQFPTQ ALCAMQGCCW NPRNESPTPW CSFANNHGYE 100
FEKISNPNIN FEPNLKKNSP PTLFGDNITN LLLTTQSQTA NRFRFKITDP 150
NNQRYEVPHQ FVNKDFSGPP ASNPLYDVKI TENPFSIKVI RKSNNKILFD 200
TSIGPLVYSN QYLQISTKLP SKYIYGLGEH VHKRFRHDLY WKTWPIFTRD 250
QLPGDNNNNL YGHQTFFMSI EDTSGKSFGV FLMNSNAMEV FIQPTPIVTY 300
RVIGGILDFY IFLGDTPGQV VQQYQELTGR PAMPSYWSLG FQLSRWNYGS 350
LDAVKEVVKR NRDARIPFDA QVTDIDYMED KKDFTYNNKT FYGLPEFVKD 400
LHDHGQKYII ILDPAISITS LANGNHYKTY ERGNEQKVWV YQSDGTTPLI 450
GEVWPGLTVY PDFTNPKCLD WWTNECSIFH EEIKYDGLWI DMNEVSSFVH 500
GSTKGCSDNK LNYPPFIPDI LDKLMYAKTI CMDAIQHWGK QYDVHSLYGY 550
SMAIATEKAI EKVFPNKRSF ILTRSTFAGT GKHATHWLGD NTPSWEHMEW 600
SITPMLEFGL FGMPFIGADI CGFVVDTTEE LCRRWMQIGA FYPYFRDHNA 650
GGYMPQDPAY FGQDSLLVNT SRHYLDIWYT LLPYLYNLLY KAYVYGETVA 700
RPFLYEFYED TNSWIEDLQF LWGSALLITP VLRQGADRMS AYIPDATWYD 750
YETGGKRTWR KQRVEMYLPG DKIGLHVRGG YIIPTQQPAV NTTASRKNPL 800
GLIIALDNNA AKGDFFWDDG ESKDSIEKGK YILYTFSVLN NELDIICTHS 850
SYQEGTTLAF ETIKILGLAN TVTQVQVAEN NQQTIIHNSF TYHASNQSLI 900
IDNLKLNLGK NFTVQWNQVS LDSEKIDCFP DNNPENKQNC EERGCLWEPN 950
SAAEGPRCYF PKQYNPYLVK STQYSSMGIT VDLELNTATA RIKMPSNPIS 1000
VLRLEVKYHK NDMLQFKIYD PQNKRYEVPI PMDIPTTPTS TYENRLYDVN 1050
IKGNPFGIQI RRRSTGRIFW DSCLPWGLLL MNQFIQISTR LPSEYVYGFG 1100
GVGHRQFKQD LNWHKWGMFN RDQPSGYKIS SYGFQPYIYM ALGDGGNAHG 1150
VFLLNSNAMD VTFQPNPALT YRTIGGILDF YMFLGPNPEV ATKQYHEVIG 1200
RPVKPPYWAL GFHLCRYGYE NTSEIRQLYE DMVSAQIPYD VQYTDIDYME 1250
RQLDFTIGKG FQDLPEFVDK IRDEGMKYII ILDPAISGNE TQDYLAFQRG 1300
IEKDVFVKWP NTQDICWAKV WPDLPNITID DSLTEDEAVN ASRAHVAFPD 1350
FLKTSTAEWW ATEIEDFYNT YMKFDGLWID MNEPSSFVHG SVDNKCRNEI 1400
LNYPPYMPAL TKRNEGLHFR TMCMETQQTL SNGSSVLHYD VHNLYGWSQA 1450
KPTYDALQKT TGKRGIVISR STYPSAGRWA GHWLGDNYAN WDKIGKSIIG 1500
MMEFSLFGIS FTGADICGFF NNSDYELCAR WMQVGAFYPY SRNHNITDTR 1550
RQDPVSWNET FASMSTDILN IRYNLLPYFY TQMHDIHANG GTVIRPLLHE 1600
FFSETGTWDI YKQFLWGPAF MVTPVVEPYS ESVTGYVPDG RWLDYHTGQD 1650
IGLRKRLHTL DAPLYKINLH VCGGHILPCQ EPAQNTYFSR QNYMKLIVAA 1700
DDNQTAQGYL FWDDGESIDT YEKGQYLLVQ FNLNKATLTS TILKNGYINT 1750
REMRLGFINV WGKGNTVVQE VNITYKGNKE SVKFSQEANK QILNIDLTAN 1800
NIVLDEPIEI SWT 1813
Length:1,813
Mass (Da):208,305
Last modified:January 23, 2007 - v3
Checksum:i93DAE1B3952C7AD6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB011401 mRNA. Translation: BAA25370.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB011401 mRNA. Translation: BAA25370.1 .

3D structure databases

ProteinModelPortali O62653.
SMRi O62653. Positions 56-917.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH31. Glycoside Hydrolase Family 31.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG080721.

Enzyme and pathway databases

SABIO-RK O62653.

Family and domain databases

Gene3Di 4.10.110.10. 2 hits.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
IPR000519. P_trefoil.
IPR017957. P_trefoil_CS.
[Graphical view ]
Pfami PF01055. Glyco_hydro_31. 2 hits.
PF00088. Trefoil. 2 hits.
[Graphical view ]
SMARTi SM00018. PD. 2 hits.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 4 hits.
SSF74650. SSF74650. 2 hits.
PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 2 hits.
PS00707. GLYCOSYL_HYDROL_F31_2. 2 hits.
PS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of sucrase-isomaltase cDNA in the house musk shrew Suncus murinus and identification of a mutation responsible for isolated sucrase deficiency."
    Ito T., Hayashi Y., Ohmori S., Oda S., Seo H.
    J. Biol. Chem. 273:16464-16469(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiSUIS_SUNMU
AccessioniPrimary (citable) accession number: O62653
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There is a high degree of homology between the isomaltase and sucrase portions (41% of amino acid identity) indicating that this protein is evolved by partial gene duplication.

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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