Reviewed, UniProtKB/Swiss-Prot O62621 (COPB2_DROME)

Names and origin

Protein names

Recommended name:
    Coatomer subunit beta'
Alternative name(s):
    Beta'-coat protein
      Short name=Beta'-COP

Gene names

Name:	beta'Cop
ORF Names:	CG6699

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	914 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins By similarity.
Subunit structure	Oligomeric complex that consist of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits By similarity.
Subcellular location	Cytoplasm By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasmic vesicle › COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it By similarity.
Sequence similarities	Contains 7 WD repeats.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Cytoplasmic vesicle Golgi apparatus Membrane
Domain	Repeat WD repeat
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Non-traceable author statement. Source: UniProtKB intracellular protein transport Inferred from electronic annotation. Source: InterPro retrograde vesicle-mediated transport, Golgi to ER Non-traceable author statement. Source: UniProtKB
Cellular component	COPI vesicle coat Non-traceable author statement. Source: UniProtKB
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 914	914	Coatomer subunit beta'		PRO_0000050916
Regions
	Repeat	13 – 54	42	WD 1
	Repeat	55 – 94	40	WD 2
	Repeat	97 – 136	40	WD 3
	Repeat	140 – 180	41	WD 4
	Repeat	183 – 224	42	WD 5
	Repeat	227 – 266	40	WD 6
	Repeat	352 – 390	39	WD 7
Experimental info
	Sequence conflict	254	1	R → C in CAA07084. Ref.1
	Sequence conflict	254	1	R → C in CAA07085. Ref.1
	Sequence conflict	343 – 345	3	LPV → CPF in CAA07084. Ref.1
	Sequence conflict	343 – 345	3	LPV → CPF in CAA07085. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O62621-1 [UniParc]. Last modified January 27, 2003. Version 2. Checksum: 15E862E1944F5C4B	FASTA	914	102,713
	10 20 30 40 50 60 MPLKLDIKRR LTSRSDRVKC VDLHPAEPWM LCALYNGHVH IMNYENQQMV KDFEVCDVPV 70 80 90 100 110 120 RSARFVARKN WILTGSDDMQ IRVFNYNTLE KVHSFEAHSD YLRCIAVHPT QPLVLTSSDD 130 140 150 160 170 180 MLIKLWNWEK MWACQRVFEG HTHYVMQIVF NPKDNNTFAS ASLDRTVKVW QLGSNFANFT 190 200 210 220 230 240 LEGHEKGVNC VDYYHGGDKP YLISGADDRL VKIWDYQNKT CVQTLEGHAQ NISAVCFHPE 250 260 270 280 290 300 LPIVLTGSED GTVRIWHSGT YRLETCLNYG FERVWTISSM RGTNNVALGY DEGSIIIKVG 310 320 330 340 350 360 REEPAMSMDV VGSKIIWAKH SEMQQVNLKT IADGTEIKDG ERLPVATKDM GACEIYPQTI 370 380 390 400 410 420 AHNPNGRFVV VCGDGEYIIY TSMALRNKAF GSAQEFVWAL ESNEYAIREN NGTVRLFRNF 430 440 450 460 470 480 KERKSFTPEY GAESIYGGYY FGVKTSSGLA FYDWETLQLV RRIEVQPKNV FWNESGSLVC 490 500 510 520 530 540 LATDDSYFVL GVDTAQVANA VETKEGLEDD GVESAFNVLG EVSECVKTGL WVGDCFIYTN 550 560 570 580 590 600 SVNRINYYVG GEIVTVSHLD RTMYLLGYVP KDNRIYLGDK ELNVISFCLQ LSVLEYQTAV 610 620 630 640 650 660 MRRDFERADV VLPTIPKEHR TRVAHFLEKQ GFKSQALQVS TDADHKFDLA LQIGDLEIAL 670 680 690 700 710 720 KLARESENSQ KWSQLADVAS SKNNMSLVKE CMQKANDLSG LLLLSTASGD AQLLEVVGAA 730 740 750 760 770 780 GSAQGHHNLA FLSAFLRSDV ERCLEILIET NRLPEAAFFA RTYLPSQMSR IVELWREKLG 790 800 810 820 830 840 KVNEKAGQSL ADPAQYTNLF PGLGDALRVE QHLQEERARK APARLAANLP LNSERHPLQE 850 860 870 880 890 900 LFAAEQGAAG QHLEEKVKPA YVPAQAVVSS SQVAEPTSAA DDDDDLDLEI DGITLDDNID 910 TTDVNLDDDF LSDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the Drosophila coatomer subunit beta'." Merdes G., Heid H.W., Mechler B.M. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 613-914. Strain: Berkeley. Tissue: Head.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ006523 mRNA. Translation: CAA07084.1. AJ006524 Genomic DNA. Translation: CAA07085.1. AE014134 Genomic DNA. Translation: AAF53294.2. BT023906 mRNA. Translation: ABA81840.1. AY119527 mRNA. Translation: AAM50181.1. Different initiation.
RefSeq	NP_524836.2.
UniGene	Dm.2980
3D structure databases
HSSP	HSSP built from PDB template 1ERJ based on UniProtKB P16649.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:22262N.
IntAct	O62621. 1 interaction.
Proteomic databases
PRIDE	O62621.
Genome annotation databases
Ensembl	FBgn0025724. Drosophila melanogaster. [Contig view]
GeneID	45757.
KEGG	dme:Dmel_CG6699.
NMPDR	fig|7227.3.peg.2269.
Organism-specific databases
FlyBase	FBgn0025724. beta'Cop.
Phylogenomic databases
HOGENOM	O62621.
OMA	O62621. FYDWENT.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-008806-MON.
Gene expression databases
ArrayExpress	O62621.
GermOnline	CG6699. Drosophila melanogaster.
Family and domain databases
InterPro	IPR016453. Coatomer_beta'_subunit. IPR006692. Coatomer_WD-assoc_reg. IPR015943. WD40/YVTN_repeat-like. IPR001680. WD40_repeat. IPR019782. WD40_repeat_2. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_region. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF04053. Coatomer_WDAD. 1 hit. PF00400. WD40. 5 hits. [Graphical view]
PIRSF	PIRSF005567. Coatomer_beta'_subunit. 1 hit.
ProDom	PD000018. WD40. 4 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00320. WD40. 6 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. 4 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	838315.

Entry information

Entry name

COPB2_DROME

Accession

Primary (citable) accession number: O62621
Secondary accession number(s): Q3KN33, Q8MRM3, Q9VJZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 27, 2003
Last modified:	June 16, 2009
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project