Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Coatomer subunit beta'

Gene

beta'COP

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-DME-6807878. COPI-mediated anterograde transport.
R-DME-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
SignaLinkiO62621.

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit beta'
Alternative name(s):
Beta'-coat protein
Short name:
Beta'-COP
Gene namesi
Name:beta'COPImported
ORF Names:CG6699Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0025724. beta'COP.

Subcellular locationi

GO - Cellular componenti

  • COPI vesicle coat Source: FlyBase
  • Golgi stack Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 914914Coatomer subunit beta'PRO_0000050916Add
BLAST

Proteomic databases

PaxDbiO62621.
PRIDEiO62621.

Expressioni

Gene expression databases

BgeeiO62621.
GenevisibleiO62621. DM.

Interactioni

Subunit structurei

Oligomeric complex that consist of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.By similarity

Protein-protein interaction databases

BioGridi69859. 3 interactions.
DIPiDIP-22262N.
IntActiO62621. 3 interactions.
MINTiMINT-1636818.
STRINGi7227.FBpp0080048.

Structurei

3D structure databases

ProteinModelPortaliO62621.
SMRiO62621. Positions 1-803.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati13 – 5442WD 1Add
BLAST
Repeati55 – 9440WD 2Add
BLAST
Repeati97 – 13640WD 3Add
BLAST
Repeati140 – 18041WD 4Add
BLAST
Repeati183 – 22442WD 5Add
BLAST
Repeati227 – 26640WD 6Add
BLAST
Repeati352 – 39039WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat COPB2 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0276. Eukaryota.
ENOG410XNNY. LUCA.
GeneTreeiENSGT00780000122009.
InParanoidiO62621.
KOiK17302.
OMAiNWVITGA.
OrthoDBiEOG78PV85.
PhylomeDBiO62621.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR006692. Coatomer_WD-assoc_reg.
IPR016453. COPB2.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF04053. Coatomer_WDAD. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PIRSFiPIRSF005567. Coatomer_beta'_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
PROSITEiPS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O62621-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLKLDIKRR LTSRSDRVKC VDLHPAEPWM LCALYNGHVH IMNYENQQMV
60 70 80 90 100
KDFEVCDVPV RSARFVARKN WILTGSDDMQ IRVFNYNTLE KVHSFEAHSD
110 120 130 140 150
YLRCIAVHPT QPLVLTSSDD MLIKLWNWEK MWACQRVFEG HTHYVMQIVF
160 170 180 190 200
NPKDNNTFAS ASLDRTVKVW QLGSNFANFT LEGHEKGVNC VDYYHGGDKP
210 220 230 240 250
YLISGADDRL VKIWDYQNKT CVQTLEGHAQ NISAVCFHPE LPIVLTGSED
260 270 280 290 300
GTVRIWHSGT YRLETCLNYG FERVWTISSM RGTNNVALGY DEGSIIIKVG
310 320 330 340 350
REEPAMSMDV VGSKIIWAKH SEMQQVNLKT IADGTEIKDG ERLPVATKDM
360 370 380 390 400
GACEIYPQTI AHNPNGRFVV VCGDGEYIIY TSMALRNKAF GSAQEFVWAL
410 420 430 440 450
ESNEYAIREN NGTVRLFRNF KERKSFTPEY GAESIYGGYY FGVKTSSGLA
460 470 480 490 500
FYDWETLQLV RRIEVQPKNV FWNESGSLVC LATDDSYFVL GVDTAQVANA
510 520 530 540 550
VETKEGLEDD GVESAFNVLG EVSECVKTGL WVGDCFIYTN SVNRINYYVG
560 570 580 590 600
GEIVTVSHLD RTMYLLGYVP KDNRIYLGDK ELNVISFCLQ LSVLEYQTAV
610 620 630 640 650
MRRDFERADV VLPTIPKEHR TRVAHFLEKQ GFKSQALQVS TDADHKFDLA
660 670 680 690 700
LQIGDLEIAL KLARESENSQ KWSQLADVAS SKNNMSLVKE CMQKANDLSG
710 720 730 740 750
LLLLSTASGD AQLLEVVGAA GSAQGHHNLA FLSAFLRSDV ERCLEILIET
760 770 780 790 800
NRLPEAAFFA RTYLPSQMSR IVELWREKLG KVNEKAGQSL ADPAQYTNLF
810 820 830 840 850
PGLGDALRVE QHLQEERARK APARLAANLP LNSERHPLQE LFAAEQGAAG
860 870 880 890 900
QHLEEKVKPA YVPAQAVVSS SQVAEPTSAA DDDDDLDLEI DGITLDDNID
910
TTDVNLDDDF LSDD
Length:914
Mass (Da):102,713
Last modified:January 27, 2003 - v2
Checksum:i15E862E1944F5C4B
GO

Sequence cautioni

The sequence AAM50181.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti254 – 2541R → C in CAA07084 (Ref. 1) Curated
Sequence conflicti254 – 2541R → C in CAA07085 (Ref. 1) Curated
Sequence conflicti343 – 3453LPV → CPF in CAA07084 (Ref. 1) Curated
Sequence conflicti343 – 3453LPV → CPF in CAA07085 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006523 mRNA. Translation: CAA07084.1.
AJ006524 Genomic DNA. Translation: CAA07085.1.
AE014134 Genomic DNA. Translation: AAF53294.2.
BT023906 mRNA. Translation: ABA81840.1.
AY119527 mRNA. Translation: AAM50181.1. Different initiation.
RefSeqiNP_524836.2. NM_080097.4.
UniGeneiDm.2980.

Genome annotation databases

EnsemblMetazoaiFBtr0080469; FBpp0080048; FBgn0025724.
GeneIDi45757.
KEGGidme:Dmel_CG6699.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006523 mRNA. Translation: CAA07084.1.
AJ006524 Genomic DNA. Translation: CAA07085.1.
AE014134 Genomic DNA. Translation: AAF53294.2.
BT023906 mRNA. Translation: ABA81840.1.
AY119527 mRNA. Translation: AAM50181.1. Different initiation.
RefSeqiNP_524836.2. NM_080097.4.
UniGeneiDm.2980.

3D structure databases

ProteinModelPortaliO62621.
SMRiO62621. Positions 1-803.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi69859. 3 interactions.
DIPiDIP-22262N.
IntActiO62621. 3 interactions.
MINTiMINT-1636818.
STRINGi7227.FBpp0080048.

Proteomic databases

PaxDbiO62621.
PRIDEiO62621.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0080469; FBpp0080048; FBgn0025724.
GeneIDi45757.
KEGGidme:Dmel_CG6699.

Organism-specific databases

CTDi45757.
FlyBaseiFBgn0025724. beta'COP.

Phylogenomic databases

eggNOGiKOG0276. Eukaryota.
ENOG410XNNY. LUCA.
GeneTreeiENSGT00780000122009.
InParanoidiO62621.
KOiK17302.
OMAiNWVITGA.
OrthoDBiEOG78PV85.
PhylomeDBiO62621.

Enzyme and pathway databases

ReactomeiR-DME-6807878. COPI-mediated anterograde transport.
R-DME-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
SignaLinkiO62621.

Miscellaneous databases

ChiTaRSibeta'Cop. fly.
GenomeRNAii45757.
PROiO62621.

Gene expression databases

BgeeiO62621.
GenevisibleiO62621. DM.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR006692. Coatomer_WD-assoc_reg.
IPR016453. COPB2.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF04053. Coatomer_WDAD. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PIRSFiPIRSF005567. Coatomer_beta'_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
PROSITEiPS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the Drosophila coatomer subunit beta'."
    Merdes G., Heid H.W., Mechler B.M.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Tissue: Embryo.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Head.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 613-914.
    Strain: Berkeley.
    Tissue: Head.

Entry informationi

Entry nameiCOPB2_DROME
AccessioniPrimary (citable) accession number: O62621
Secondary accession number(s): Q3KN33, Q8MRM3, Q9VJZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 27, 2003
Last modified: July 6, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.