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O62619 (KPYK_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:PyK
ORF Names:CG7070
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium.

Potassium.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Tissue specificity

In adults expressed predominantly in the thorax. Ref.6

Developmental stage

Low levels in larvae and pupae, but increases in young adults, becoming relatively stable in two-day-old flies. Ref.6

Sequence similarities

Belongs to the pyruvate kinase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: O62619-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: O62619-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 533533Pyruvate kinase
PRO_0000112099

Sites

Metal binding781Potassium By similarity
Metal binding801Potassium By similarity
Metal binding1161Potassium By similarity
Metal binding1171Potassium; via carbonyl oxygen By similarity
Metal binding2751Magnesium By similarity
Metal binding2991Magnesium By similarity
Binding site761Substrate By similarity
Binding site2981Substrate; via amide nitrogen By similarity
Binding site2991Substrate; via amide nitrogen By similarity
Binding site3311Substrate By similarity
Site2731Transition state stabilizer By similarity

Natural variations

Alternative sequence1 – 2121Missing in isoform B.
VSP_002885

Experimental info

Sequence conflict1801D → N in AAC16244. Ref.1
Sequence conflict1801D → N in AAC15808. Ref.1
Sequence conflict2711I → F in AAO24935. Ref.4
Sequence conflict3971L → F in AAC16244. Ref.1
Sequence conflict3971L → F in AAC15808. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified January 27, 2003. Version 2.
Checksum: A8376B72F040EEDB

FASTA53357,440
        10         20         30         40         50         60 
MVNVTIYDEA PQLKPNEVPQ NMAAGADTQL EHMCRLQFDS PVPHVRLSGI VCTIGPASSS 

        70         80         90        100        110        120 
VEMLEKMMAT GMNIARMNFS HGSHEYHAAT VANVRQAVKN YSAKLGYEHP VAIALDTKGP 

       130        140        150        160        170        180 
EIRTGLIGGS GTAEIELKKG EKIKLTTNKE FLEKGSLEIV YVDYENIVNV VKPGNRVFVD 

       190        200        210        220        230        240 
DGLISLIVRE VGKDSLTCEV ENGGSLGSRK GVNLPGVPVD LPAVSEKDKS DLLFGVEQEV 

       250        260        270        280        290        300 
DMIFASFIRN AAALTEIRKV LGEKGKNIKI ISKIENQQGM HNLDEIIEAG DGIMVARGDL 

       310        320        330        340        350        360 
GIEIPAEKVF LAQKAMIARC NKAGKPVICA TQMLESMVKK PRPTRAEISD VANAVLDGAD 

       370        380        390        400        410        420 
CVMLSGETAK GEYPLECVLT MAKTCKEAEA ALWHQNLFND LVRGAGTIDA SHAAAIAAVE 

       430        440        450        460        470        480 
AATKAKASAI VVITTSGKSA FQVSKYRPRC PIIAVTRFAQ TARQAHLYRG LVPLIYKEPG 

       490        500        510        520        530 
LGDWLKDVDV RVQFGLQVGK KNGFIKTGDS VVVVTGWKQG SGFTNTIRIV TVE 

« Hide

Isoform B [UniParc].

Checksum: 4ED4A31F0686874B
Show »

FASTA51255,059

References

« Hide 'large scale' references
[1]"Complementary DNA cloning and analysis of gene structure of pyruvate kinase from Drosophila melanogaster."
Chien Y.-C., Zhu Y.-J., Chuen C.-M.
Zool. Stud. 38:322-332(1999)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM A).
Strain: Canton-S.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B).
Strain: Berkeley.
Tissue: Embryo and Head.
[5]Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.
Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
Strain: Berkeley.
[6]"Localization of a dosage sensitive region for pyruvate kinase in Drosophila melanogaster."
Rust K.J., Collier G.E.
J. Hered. 76:39-44(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF062478 Genomic DNA. Translation: AAC16244.1.
AF061507 mRNA. Translation: AAC15808.1.
AE014297 Genomic DNA. Translation: AAF55979.3.
AE014297 Genomic DNA. Translation: AAN14373.1.
AY118442 mRNA. Translation: AAM48471.1.
BT003180 mRNA. Translation: AAO24935.1.
BT082045 mRNA. Translation: ACO95723.1.
RefSeqNP_524448.3. NM_079724.2. [O62619-1]
NP_732723.1. NM_170004.1. [O62619-2]
UniGeneDm.7108.

3D structure databases

ProteinModelPortalO62619.
SMRO62619. Positions 30-532.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid67578. 14 interactions.
DIPDIP-19290N.
IntActO62619. 3 interactions.

Proteomic databases

PaxDbO62619.
PRIDEO62619.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0084214; FBpp0083611; FBgn0003178. [O62619-1]
GeneID42620.
KEGGdme:Dmel_CG7070.

Organism-specific databases

CTD42620.
FlyBaseFBgn0003178. PyK.

Phylogenomic databases

eggNOGCOG0469.
GeneTreeENSGT00390000008859.
InParanoidO62619.
KOK00873.
OMAFIPANSE.
OrthoDBEOG78M01Q.
PhylomeDBO62619.

Enzyme and pathway databases

UniPathwayUPA00109; UER00188.

Gene expression databases

BgeeO62619.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi42620.
NextBio829729.
PROO62619.

Entry information

Entry nameKPYK_DROME
AccessionPrimary (citable) accession number: O62619
Secondary accession number(s): C3KGI0 expand/collapse secondary AC list , Q86PE3, Q8MT14, Q9VD24
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 27, 2003
Last modified: July 9, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase