Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyruvate kinase

Gene

PyK

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (Gapdh1), Glyceraldehyde-3-phosphate dehydrogenase 2 (Gapdh2), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh2), Glyceraldehyde-3-phosphate dehydrogenase (CG9010)
  2. Phosphoglycerate kinase (Pgk), Phosphoglycerate kinase, Phosphoglycerate kinase (Dmel\CG9961), Phosphoglycerate kinase (Dmel\CG9961), Phosphoglycerate kinase (Pgk), Phosphoglycerate kinase (CG9961)
  3. no protein annotated in this organism
  4. Enolase (Eno)
  5. Pyruvate kinase (CG7362), Pyruvate kinase (PyK), Pyruvate kinase (Dmel\CG2964), Pyruvate kinase (Dmel\CG7069), Pyruvate kinase (CG7069), Pyruvate kinase (CG2964)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei76SubstrateBy similarity1
Metal bindingi78PotassiumBy similarity1
Metal bindingi80PotassiumBy similarity1
Metal bindingi116PotassiumBy similarity1
Metal bindingi117Potassium; via carbonyl oxygenBy similarity1
Sitei273Transition state stabilizerBy similarity1
Metal bindingi275MagnesiumBy similarity1
Binding sitei298Substrate; via amide nitrogenBy similarity1
Metal bindingi299MagnesiumBy similarity1
Binding sitei299Substrate; via amide nitrogenBy similarity1
Binding sitei331SubstrateBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • potassium ion binding Source: InterPro
  • pyruvate kinase activity Source: FlyBase

GO - Biological processi

  • glycolytic process Source: FlyBase
  • myoblast fusion Source: FlyBase
  • pyruvate metabolic process Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • response to sucrose Source: FlyBase
  • somatic muscle development Source: FlyBase

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

ReactomeiR-DME-6798695. Neutrophil degranulation.
R-DME-70171. Glycolysis.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:PyK
ORF Names:CG7070
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0267385. PyK.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120991 – 533Pyruvate kinaseAdd BLAST533

Proteomic databases

PaxDbiO62619.
PRIDEiO62619.

Expressioni

Tissue specificityi

In adults expressed predominantly in the thorax.1 Publication

Developmental stagei

Low levels in larvae and pupae, but increases in young adults, becoming relatively stable in two-day-old flies.1 Publication

Gene expression databases

BgeeiFBgn0267385.
GenevisibleiO62619. DM.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi67578. 30 interactors.
DIPiDIP-19290N.
IntActiO62619. 27 interactors.
STRINGi7227.FBpp0083611.

Structurei

3D structure databases

ProteinModelPortaliO62619.
SMRiO62619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
GeneTreeiENSGT00390000008859.
InParanoidiO62619.
KOiK00873.
OMAiKHEAIEQ.
OrthoDBiEOG091G0597.
PhylomeDBiO62619.

Family and domain databases

CDDicd00288. Pyruvate_Kinase. 1 hit.
Gene3Di3.40.1380.20. 1 hit.
InterProiView protein in InterPro
IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiView protein in Pfam
PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiView protein in PROSITE
PS00110. PYRUVATE_KINASE. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: O62619-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVNVTIYDEA PQLKPNEVPQ NMAAGADTQL EHMCRLQFDS PVPHVRLSGI
60 70 80 90 100
VCTIGPASSS VEMLEKMMAT GMNIARMNFS HGSHEYHAAT VANVRQAVKN
110 120 130 140 150
YSAKLGYEHP VAIALDTKGP EIRTGLIGGS GTAEIELKKG EKIKLTTNKE
160 170 180 190 200
FLEKGSLEIV YVDYENIVNV VKPGNRVFVD DGLISLIVRE VGKDSLTCEV
210 220 230 240 250
ENGGSLGSRK GVNLPGVPVD LPAVSEKDKS DLLFGVEQEV DMIFASFIRN
260 270 280 290 300
AAALTEIRKV LGEKGKNIKI ISKIENQQGM HNLDEIIEAG DGIMVARGDL
310 320 330 340 350
GIEIPAEKVF LAQKAMIARC NKAGKPVICA TQMLESMVKK PRPTRAEISD
360 370 380 390 400
VANAVLDGAD CVMLSGETAK GEYPLECVLT MAKTCKEAEA ALWHQNLFND
410 420 430 440 450
LVRGAGTIDA SHAAAIAAVE AATKAKASAI VVITTSGKSA FQVSKYRPRC
460 470 480 490 500
PIIAVTRFAQ TARQAHLYRG LVPLIYKEPG LGDWLKDVDV RVQFGLQVGK
510 520 530
KNGFIKTGDS VVVVTGWKQG SGFTNTIRIV TVE
Length:533
Mass (Da):57,440
Last modified:January 27, 2003 - v2
Checksum:iA8376B72F040EEDB
GO
Isoform B (identifier: O62619-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.

Note: No experimental confirmation available.
Show »
Length:512
Mass (Da):55,059
Checksum:i4ED4A31F0686874B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180D → N in AAC16244 (Ref. 1) Curated1
Sequence conflicti180D → N in AAC15808 (Ref. 1) Curated1
Sequence conflicti271I → F in AAO24935 (PubMed:12537569).Curated1
Sequence conflicti397L → F in AAC16244 (Ref. 1) Curated1
Sequence conflicti397L → F in AAC15808 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0028851 – 21Missing in isoform B. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062478 Genomic DNA. Translation: AAC16244.1.
AF061507 mRNA. Translation: AAC15808.1.
AE014297 Genomic DNA. Translation: AAF55979.3.
AE014297 Genomic DNA. Translation: AAN14373.1.
AY118442 mRNA. Translation: AAM48471.1.
BT003180 mRNA. Translation: AAO24935.1.
BT082045 mRNA. Translation: ACO95723.1.
RefSeqiNP_524448.3. NM_079724.3. [O62619-1]
NP_732723.1. NM_170004.2. [O62619-2]
UniGeneiDm.7108.

Genome annotation databases

EnsemblMetazoaiFBtr0084213; FBpp0083610; FBgn0267385. [O62619-2]
FBtr0084214; FBpp0083611; FBgn0267385. [O62619-1]
GeneIDi42620.
KEGGidme:Dmel_CG7070.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKPYK_DROME
AccessioniPrimary (citable) accession number: O62619
Secondary accession number(s): C3KGI0
, Q86PE3, Q8MT14, Q9VD24
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 27, 2003
Last modified: September 27, 2017
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families