Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O62619

- KPYK_DROME

UniProt

O62619 - KPYK_DROME

Protein

Pyruvate kinase

Gene

PyK

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (27 Jan 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei76 – 761SubstrateBy similarity
    Metal bindingi78 – 781PotassiumBy similarity
    Metal bindingi80 – 801PotassiumBy similarity
    Metal bindingi116 – 1161PotassiumBy similarity
    Metal bindingi117 – 1171Potassium; via carbonyl oxygenBy similarity
    Sitei273 – 2731Transition state stabilizerBy similarity
    Metal bindingi275 – 2751MagnesiumBy similarity
    Binding sitei298 – 2981Substrate; via amide nitrogenBy similarity
    Metal bindingi299 – 2991MagnesiumBy similarity
    Binding sitei299 – 2991Substrate; via amide nitrogenBy similarity
    Binding sitei331 – 3311SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: FlyBase

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    ReactomeiREACT_206319. Glycolysis.
    REACT_213793. ChREBP activates metabolic gene expression.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:PyK
    ORF Names:CG7070
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0003178. PyK.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: FlyBase
    2. lipid particle Source: FlyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 533533Pyruvate kinasePRO_0000112099Add
    BLAST

    Proteomic databases

    PaxDbiO62619.
    PRIDEiO62619.

    Expressioni

    Tissue specificityi

    In adults expressed predominantly in the thorax.1 Publication

    Developmental stagei

    Low levels in larvae and pupae, but increases in young adults, becoming relatively stable in two-day-old flies.1 Publication

    Gene expression databases

    BgeeiO62619.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    BioGridi67578. 14 interactions.
    DIPiDIP-19290N.
    IntActiO62619. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliO62619.
    SMRiO62619. Positions 30-532.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    GeneTreeiENSGT00390000008859.
    InParanoidiO62619.
    KOiK00873.
    OMAiFIPANSE.
    OrthoDBiEOG78M01Q.
    PhylomeDBiO62619.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: O62619-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVNVTIYDEA PQLKPNEVPQ NMAAGADTQL EHMCRLQFDS PVPHVRLSGI    50
    VCTIGPASSS VEMLEKMMAT GMNIARMNFS HGSHEYHAAT VANVRQAVKN 100
    YSAKLGYEHP VAIALDTKGP EIRTGLIGGS GTAEIELKKG EKIKLTTNKE 150
    FLEKGSLEIV YVDYENIVNV VKPGNRVFVD DGLISLIVRE VGKDSLTCEV 200
    ENGGSLGSRK GVNLPGVPVD LPAVSEKDKS DLLFGVEQEV DMIFASFIRN 250
    AAALTEIRKV LGEKGKNIKI ISKIENQQGM HNLDEIIEAG DGIMVARGDL 300
    GIEIPAEKVF LAQKAMIARC NKAGKPVICA TQMLESMVKK PRPTRAEISD 350
    VANAVLDGAD CVMLSGETAK GEYPLECVLT MAKTCKEAEA ALWHQNLFND 400
    LVRGAGTIDA SHAAAIAAVE AATKAKASAI VVITTSGKSA FQVSKYRPRC 450
    PIIAVTRFAQ TARQAHLYRG LVPLIYKEPG LGDWLKDVDV RVQFGLQVGK 500
    KNGFIKTGDS VVVVTGWKQG SGFTNTIRIV TVE 533
    Length:533
    Mass (Da):57,440
    Last modified:January 27, 2003 - v2
    Checksum:iA8376B72F040EEDB
    GO
    Isoform B (identifier: O62619-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-21: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:512
    Mass (Da):55,059
    Checksum:i4ED4A31F0686874B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti180 – 1801D → N in AAC16244. 1 PublicationCurated
    Sequence conflicti180 – 1801D → N in AAC15808. 1 PublicationCurated
    Sequence conflicti271 – 2711I → F in AAO24935. (PubMed:12537569)Curated
    Sequence conflicti397 – 3971L → F in AAC16244. 1 PublicationCurated
    Sequence conflicti397 – 3971L → F in AAC15808. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2121Missing in isoform B. 1 PublicationVSP_002885Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF062478 Genomic DNA. Translation: AAC16244.1.
    AF061507 mRNA. Translation: AAC15808.1.
    AE014297 Genomic DNA. Translation: AAF55979.3.
    AE014297 Genomic DNA. Translation: AAN14373.1.
    AY118442 mRNA. Translation: AAM48471.1.
    BT003180 mRNA. Translation: AAO24935.1.
    BT082045 mRNA. Translation: ACO95723.1.
    RefSeqiNP_524448.3. NM_079724.2. [O62619-1]
    NP_732723.1. NM_170004.1. [O62619-2]
    UniGeneiDm.7108.

    Genome annotation databases

    EnsemblMetazoaiFBtr0084214; FBpp0083611; FBgn0003178. [O62619-1]
    GeneIDi42620.
    KEGGidme:Dmel_CG7070.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF062478 Genomic DNA. Translation: AAC16244.1 .
    AF061507 mRNA. Translation: AAC15808.1 .
    AE014297 Genomic DNA. Translation: AAF55979.3 .
    AE014297 Genomic DNA. Translation: AAN14373.1 .
    AY118442 mRNA. Translation: AAM48471.1 .
    BT003180 mRNA. Translation: AAO24935.1 .
    BT082045 mRNA. Translation: ACO95723.1 .
    RefSeqi NP_524448.3. NM_079724.2. [O62619-1 ]
    NP_732723.1. NM_170004.1. [O62619-2 ]
    UniGenei Dm.7108.

    3D structure databases

    ProteinModelPortali O62619.
    SMRi O62619. Positions 30-532.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 67578. 14 interactions.
    DIPi DIP-19290N.
    IntActi O62619. 3 interactions.

    Proteomic databases

    PaxDbi O62619.
    PRIDEi O62619.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0084214 ; FBpp0083611 ; FBgn0003178 . [O62619-1 ]
    GeneIDi 42620.
    KEGGi dme:Dmel_CG7070.

    Organism-specific databases

    CTDi 42620.
    FlyBasei FBgn0003178. PyK.

    Phylogenomic databases

    eggNOGi COG0469.
    GeneTreei ENSGT00390000008859.
    InParanoidi O62619.
    KOi K00873.
    OMAi FIPANSE.
    OrthoDBi EOG78M01Q.
    PhylomeDBi O62619.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    Reactomei REACT_206319. Glycolysis.
    REACT_213793. ChREBP activates metabolic gene expression.

    Miscellaneous databases

    GenomeRNAii 42620.
    NextBioi 829729.
    PROi O62619.

    Gene expression databases

    Bgeei O62619.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complementary DNA cloning and analysis of gene structure of pyruvate kinase from Drosophila melanogaster."
      Chien Y.-C., Zhu Y.-J., Chuen C.-M.
      Zool. Stud. 38:322-332(1999)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM A).
      Strain: Canton-S.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B).
      Strain: Berkeley.
      Tissue: Embryo and Head.
    5. Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.
      Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
      Strain: Berkeley.
    6. "Localization of a dosage sensitive region for pyruvate kinase in Drosophila melanogaster."
      Rust K.J., Collier G.E.
      J. Hered. 76:39-44(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiKPYK_DROME
    AccessioniPrimary (citable) accession number: O62619
    Secondary accession number(s): C3KGI0
    , Q86PE3, Q8MT14, Q9VD24
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 27, 2003
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3