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Protein

Mitogen-activated protein kinase 14A

Gene

Mpk2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Kinase involved in a signal transduction pathway. May down-regulate insect immunity gene expression after prolonged infection.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation by Mkk3 in response to environmental stress.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei154Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: FlyBase
  • protein serine/threonine kinase activity Source: FlyBase
  • SAP kinase activity Source: FlyBase

GO - Biological processi

  • cellular response to arsenic-containing substance Source: FlyBase
  • cellular response to cadmium ion Source: FlyBase
  • cellular response to reactive oxygen species Source: FlyBase
  • defense response to bacterium Source: FlyBase
  • defense response to fungus Source: FlyBase
  • determination of adult lifespan Source: FlyBase
  • heart morphogenesis Source: FlyBase
  • immune response Source: FlyBase
  • MAPK cascade Source: FlyBase
  • mucosal immune response Source: FlyBase
  • negative regulation of antimicrobial humoral response Source: FlyBase
  • paracrine signaling Source: FlyBase
  • positive regulation of cell size Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • reactive oxygen species metabolic process Source: FlyBase
  • regulation of adult chitin-containing cuticle pigmentation Source: FlyBase
  • regulation of cellular response to oxidative stress Source: FlyBase
  • response to heat Source: FlyBase
  • response to hydrogen peroxide Source: FlyBase
  • response to osmotic stress Source: FlyBase
  • response to oxidative stress Source: FlyBase
  • response to starvation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 1994.
ReactomeiR-DME-168638. NOD1/2 Signaling Pathway.
R-DME-171007. p38MAPK events.
R-DME-198753. ERK/MAPK targets.
R-DME-2559580. Oxidative Stress Induced Senescence.
R-DME-375170. CDO in myogenesis.
R-DME-376172. DSCAM interactions.
R-DME-418592. ADP signalling through P2Y purinoceptor 1.
R-DME-432142. Platelet sensitization by LDL.
R-DME-4420097. VEGFA-VEGFR2 Pathway.
R-DME-450302. activated TAK1 mediates p38 MAPK activation.
R-DME-6798695. Neutrophil degranulation.
SignaLinkiO62618.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 14A (EC:2.7.11.24)
Short name:
MAP kinase 14A
Short name:
MAPK 14A
Alternative name(s):
MAP kinase p38a
Short name:
D-p38a
p38 MAPK
Short name:
Dp38
Gene namesi
Name:Mpk2
Synonyms:p38a
ORF Names:CG5475
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0015765. Mpk2.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863001 – 366Mitogen-activated protein kinase 14AAdd BLAST366

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei184PhosphothreonineBy similarity1
Modified residuei186Phosphotyrosine1 Publication1

Post-translational modificationi

Dually phosphorylated on Thr-184 and Tyr-186, which activates the enzyme.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO62618.
PRIDEiO62618.

PTM databases

iPTMnetiO62618.

Expressioni

Developmental stagei

Expressed both maternally and zygotically. Levels are highest at the preblastoderm stage but low levels are present throughout development.1 Publication

Gene expression databases

BgeeiFBgn0015765.
ExpressionAtlasiO62618. baseline.
GenevisibleiO62618. DM.

Interactioni

Protein-protein interaction databases

BioGridi67799. 4 interactors.
IntActiO62618. 2 interactors.
MINTiMINT-4080391.
STRINGi7227.FBpp0083966.

Structurei

3D structure databases

ProteinModelPortaliO62618.
SMRiO62618.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 312Protein kinasePROSITE-ProRule annotationAdd BLAST288

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi184 – 186TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
GeneTreeiENSGT00550000074271.
InParanoidiO62618.
KOiK04441.
OMAiEMSQERP.
OrthoDBiEOG091G08QL.
PhylomeDBiO62618.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O62618-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSITKKFY KLDINRTEWE IPDIYQDLQP VGSGAYGQVS KAVVRGTNMH
60 70 80 90 100
VAIKKLARPF QSAVHAKRTY RELRLLKHMD HENVIGLLDI FHPHPANGSL
110 120 130 140 150
ENFQQVYLVT HLMDADLNNI IRMQHLSDDH VQFLVYQILR GLKYIHSAGV
160 170 180 190 200
IHRDLKPSNI AVNEDCELRI LDFGLARPTE NEMTGYVATR WYRAPEIMLN
210 220 230 240 250
WMHYDQTVDI WSVGCIMAEL ITRRTLFPGT DHIHQLNLIM EMLGTPPAEF
260 270 280 290 300
LKKISSESAR SYIQSLPPMK GRSFKNVFKN ANPLAIDLLE KMLELDAEKR
310 320 330 340 350
ITAEEALSHP YLEKYAEPSV EQTSPPYDHS FEDMDLPVDK WKELIYKEVT
360
NFKPPPSYAQ VLKDVK
Length:366
Mass (Da):42,256
Last modified:August 1, 1998 - v1
Checksum:iB3592B869F97990E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27D → G in AAB97138 (PubMed:9584193).Curated1
Sequence conflicti77K → R (PubMed:1378625).Curated1
Sequence conflicti80D → A in AAB97138 (PubMed:9584193).Curated1
Sequence conflicti108L → LL (PubMed:1378625).Curated1
Sequence conflicti124Q → QQ (PubMed:1378625).Curated1
Sequence conflicti149Missing (PubMed:1378625).Curated1
Sequence conflicti163N → NN (PubMed:1378625).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86867 mRNA. Translation: AAB97138.1.
AF035546 Genomic DNA. Translation: AAC39030.1.
AF035547 mRNA. Translation: AAC39031.1.
AE014297 Genomic DNA. Translation: AAF56244.1.
AE014297 Genomic DNA. Translation: AAN13984.1.
AY071670 mRNA. Translation: AAL49292.1.
RefSeqiNP_001163711.1. NM_001170240.2.
NP_477163.1. NM_057815.5.
NP_732959.1. NM_170126.5.
UniGeneiDm.2996.

Genome annotation databases

EnsemblMetazoaiFBtr0084580; FBpp0083965; FBgn0015765.
FBtr0084581; FBpp0083966; FBgn0015765.
FBtr0300572; FBpp0289799; FBgn0015765.
GeneIDi42866.
KEGGidme:Dmel_CG5475.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86867 mRNA. Translation: AAB97138.1.
AF035546 Genomic DNA. Translation: AAC39030.1.
AF035547 mRNA. Translation: AAC39031.1.
AE014297 Genomic DNA. Translation: AAF56244.1.
AE014297 Genomic DNA. Translation: AAN13984.1.
AY071670 mRNA. Translation: AAL49292.1.
RefSeqiNP_001163711.1. NM_001170240.2.
NP_477163.1. NM_057815.5.
NP_732959.1. NM_170126.5.
UniGeneiDm.2996.

3D structure databases

ProteinModelPortaliO62618.
SMRiO62618.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi67799. 4 interactors.
IntActiO62618. 2 interactors.
MINTiMINT-4080391.
STRINGi7227.FBpp0083966.

PTM databases

iPTMnetiO62618.

Proteomic databases

PaxDbiO62618.
PRIDEiO62618.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0084580; FBpp0083965; FBgn0015765.
FBtr0084581; FBpp0083966; FBgn0015765.
FBtr0300572; FBpp0289799; FBgn0015765.
GeneIDi42866.
KEGGidme:Dmel_CG5475.

Organism-specific databases

CTDi42866.
FlyBaseiFBgn0015765. Mpk2.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
GeneTreeiENSGT00550000074271.
InParanoidiO62618.
KOiK04441.
OMAiEMSQERP.
OrthoDBiEOG091G08QL.
PhylomeDBiO62618.

Enzyme and pathway databases

BRENDAi2.7.11.24. 1994.
ReactomeiR-DME-168638. NOD1/2 Signaling Pathway.
R-DME-171007. p38MAPK events.
R-DME-198753. ERK/MAPK targets.
R-DME-2559580. Oxidative Stress Induced Senescence.
R-DME-375170. CDO in myogenesis.
R-DME-376172. DSCAM interactions.
R-DME-418592. ADP signalling through P2Y purinoceptor 1.
R-DME-432142. Platelet sensitization by LDL.
R-DME-4420097. VEGFA-VEGFR2 Pathway.
R-DME-450302. activated TAK1 mediates p38 MAPK activation.
R-DME-6798695. Neutrophil degranulation.
SignaLinkiO62618.

Miscellaneous databases

GenomeRNAii42866.
PROiO62618.

Gene expression databases

BgeeiFBgn0015765.
ExpressionAtlasiO62618. baseline.
GenevisibleiO62618. DM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMK14A_DROME
AccessioniPrimary (citable) accession number: O62618
Secondary accession number(s): A4V3C0, O46216, Q9TXB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.