Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitogen-activated protein kinase p38a

Gene

p38a

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Kinase involved in a signal transduction pathway. May down-regulate insect immunity gene expression after prolonged infection.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation by Mkk3 in response to environmental stress.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei154Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: FlyBase
  • protein serine/threonine kinase activity Source: FlyBase
  • SAP kinase activity Source: FlyBase

GO - Biological processi

  • cellular response to arsenic-containing substance Source: FlyBase
  • cellular response to cadmium ion Source: FlyBase
  • cellular response to reactive oxygen species Source: FlyBase
  • defense response to bacterium Source: FlyBase
  • defense response to fungus Source: FlyBase
  • determination of adult lifespan Source: FlyBase
  • heart morphogenesis Source: FlyBase
  • immune response Source: FlyBase
  • MAPK cascade Source: FlyBase
  • mucosal immune response Source: FlyBase
  • negative regulation of antimicrobial humoral response Source: FlyBase
  • paracrine signaling Source: FlyBase
  • positive regulation of cell size Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • reactive oxygen species metabolic process Source: FlyBase
  • regulation of adult chitin-containing cuticle pigmentation Source: FlyBase
  • regulation of cellular response to oxidative stress Source: FlyBase
  • regulation of gene expression Source: GO_Central
  • response to heat Source: FlyBase
  • response to hydrogen peroxide Source: FlyBase
  • response to osmotic stress Source: FlyBase
  • response to oxidative stress Source: FlyBase
  • response to starvation Source: FlyBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24 1994
ReactomeiR-DME-168638 NOD1/2 Signaling Pathway
R-DME-171007 p38MAPK events
R-DME-198753 ERK/MAPK targets
R-DME-2559580 Oxidative Stress Induced Senescence
R-DME-375170 CDO in myogenesis
R-DME-418592 ADP signalling through P2Y purinoceptor 1
R-DME-432142 Platelet sensitization by LDL
R-DME-4420097 VEGFA-VEGFR2 Pathway
R-DME-450302 activated TAK1 mediates p38 MAPK activation
R-DME-6798695 Neutrophil degranulation
SignaLinkiO62618

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase p38a1 Publication (EC:2.7.11.24)
Short name:
MAP kinase p38a1 Publication
Short name:
MAPK p38a1 Publication
Alternative name(s):
MAP kinase 14AImported
p38 MAPK1 Publication
Gene namesi
Name:p38a1 PublicationImported
Synonyms:Mpk2Imported
ORF Names:CG5475Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0015765 p38a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863001 – 366Mitogen-activated protein kinase p38aAdd BLAST366

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei184PhosphothreonineBy similarity1
Modified residuei186Phosphotyrosine1 Publication1

Post-translational modificationi

Dually phosphorylated on Thr-184 and Tyr-186, which activates the enzyme.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO62618
PRIDEiO62618

PTM databases

iPTMnetiO62618

Expressioni

Developmental stagei

Expressed both maternally and zygotically. Levels are highest at the preblastoderm stage but low levels are present throughout development.1 Publication

Gene expression databases

BgeeiFBgn0015765
ExpressionAtlasiO62618 differential
GenevisibleiO62618 DM

Interactioni

Protein-protein interaction databases

BioGridi67799, 25 interactors
IntActiO62618, 3 interactors
STRINGi7227.FBpp0083966

Structurei

3D structure databases

ProteinModelPortaliO62618
SMRiO62618
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 312Protein kinasePROSITE-ProRule annotationAdd BLAST288

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi184 – 186TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
GeneTreeiENSGT00550000074271
InParanoidiO62618
KOiK04441
OMAiASDEPDC
OrthoDBiEOG091G08QL
PhylomeDBiO62618

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR008352 MAPK_p38
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR01773 P38MAPKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

O62618-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSITKKFY KLDINRTEWE IPDIYQDLQP VGSGAYGQVS KAVVRGTNMH
60 70 80 90 100
VAIKKLARPF QSAVHAKRTY RELRLLKHMD HENVIGLLDI FHPHPANGSL
110 120 130 140 150
ENFQQVYLVT HLMDADLNNI IRMQHLSDDH VQFLVYQILR GLKYIHSAGV
160 170 180 190 200
IHRDLKPSNI AVNEDCELRI LDFGLARPTE NEMTGYVATR WYRAPEIMLN
210 220 230 240 250
WMHYDQTVDI WSVGCIMAEL ITRRTLFPGT DHIHQLNLIM EMLGTPPAEF
260 270 280 290 300
LKKISSESAR SYIQSLPPMK GRSFKNVFKN ANPLAIDLLE KMLELDAEKR
310 320 330 340 350
ITAEEALSHP YLEKYAEPSV EQTSPPYDHS FEDMDLPVDK WKELIYKEVT
360
NFKPPPSYAQ VLKDVK
Length:366
Mass (Da):42,256
Last modified:August 1, 1998 - v1
Checksum:iB3592B869F97990E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27D → G in AAB97138 (PubMed:9584193).Curated1
Sequence conflicti77K → R (PubMed:1378625).Curated1
Sequence conflicti80D → A in AAB97138 (PubMed:9584193).Curated1
Sequence conflicti108L → LL (PubMed:1378625).Curated1
Sequence conflicti124Q → QQ (PubMed:1378625).Curated1
Sequence conflicti149Missing (PubMed:1378625).Curated1
Sequence conflicti163N → NN (PubMed:1378625).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86867 mRNA Translation: AAB97138.1
AF035546 Genomic DNA Translation: AAC39030.1
AF035547 mRNA Translation: AAC39031.1
AE014297 Genomic DNA Translation: AAF56244.1
AE014297 Genomic DNA Translation: AAN13984.1
AY071670 mRNA Translation: AAL49292.1
RefSeqiNP_001163711.1, NM_001170240.2
NP_477163.1, NM_057815.5
NP_732959.1, NM_170126.5
UniGeneiDm.2996

Genome annotation databases

EnsemblMetazoaiFBtr0084580; FBpp0083965; FBgn0015765
FBtr0084581; FBpp0083966; FBgn0015765
FBtr0300572; FBpp0289799; FBgn0015765
GeneIDi42866
KEGGidme:Dmel_CG5475

Similar proteinsi

Entry informationi

Entry nameiMK38A_DROME
AccessioniPrimary (citable) accession number: O62618
Secondary accession number(s): A4V3C0, O46216, Q9TXB4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: August 1, 1998
Last modified: March 28, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health