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O62618

- MK14A_DROME

UniProt

O62618 - MK14A_DROME

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Protein

Mitogen-activated protein kinase 14A

Gene

Mpk2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Kinase involved in a signal transduction pathway. May down-regulate insect immunity gene expression after prolonged infection.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation by Mkk3 in response to environmental stress.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATPPROSITE-ProRule annotation
Active sitei154 – 1541Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: FlyBase
  3. protein serine/threonine kinase activity Source: FlyBase
  4. SAP kinase activity Source: FlyBase

GO - Biological processi

  1. cellular response to arsenic-containing substance Source: FlyBase
  2. cellular response to cadmium ion Source: FlyBase
  3. cellular response to reactive oxygen species Source: FlyBase
  4. defense response to bacterium Source: FlyBase
  5. defense response to fungus Source: FlyBase
  6. determination of adult lifespan Source: FlyBase
  7. heart morphogenesis Source: FlyBase
  8. immune response Source: FlyBase
  9. MAPK cascade Source: FlyBase
  10. mucosal immune response Source: FlyBase
  11. negative regulation of antimicrobial humoral response Source: FlyBase
  12. paracrine signaling Source: FlyBase
  13. positive regulation of cell size Source: FlyBase
  14. protein phosphorylation Source: FlyBase
  15. reactive oxygen species metabolic process Source: FlyBase
  16. regulation of adult chitin-containing cuticle pigmentation Source: FlyBase
  17. regulation of cellular response to oxidative stress Source: FlyBase
  18. response to heat Source: FlyBase
  19. response to hydrogen peroxide Source: FlyBase
  20. response to osmotic stress Source: FlyBase
  21. response to oxidative stress Source: FlyBase
  22. response to starvation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 1994.
ReactomeiREACT_180302. KSRP destabilizes mRNA.
REACT_181712. Oxidative Stress Induced Senescence.
REACT_203135. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.
REACT_221889. Platelet sensitization by LDL.
REACT_246045. CDO in myogenesis.
REACT_246078. p38MAPK events.
REACT_249521. NOD1/2 Signaling Pathway.
REACT_252605. VEGFA-VEGFR2 Pathway.
REACT_255277. ADP signalling through P2Y purinoceptor 1.
REACT_257192. activated TAK1 mediates p38 MAPK activation.
REACT_263734. ERK/MAPK targets.
REACT_34513. DSCAM interactions.
SignaLinkiO62618.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 14A (EC:2.7.11.24)
Short name:
MAP kinase 14A
Short name:
MAPK 14A
Alternative name(s):
MAP kinase p38a
Short name:
D-p38a
p38 MAPK
Short name:
Dp38
Gene namesi
Name:Mpk2
Synonyms:p38a
ORF Names:CG5475
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0015765. Mpk2.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366Mitogen-activated protein kinase 14APRO_0000186300Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei184 – 1841PhosphothreonineBy similarity
Modified residuei186 – 1861Phosphotyrosine1 Publication

Post-translational modificationi

Dually phosphorylated on Thr-184 and Tyr-186, which activates the enzyme.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO62618.
PRIDEiO62618.

Expressioni

Developmental stagei

Expressed both maternally and zygotically. Levels are highest at the preblastoderm stage but low levels are present throughout development.1 Publication

Gene expression databases

BgeeiO62618.

Interactioni

Protein-protein interaction databases

BioGridi67799. 4 interactions.
IntActiO62618. 2 interactions.
MINTiMINT-4080391.
STRINGi7227.FBpp0083966.

Structurei

3D structure databases

ProteinModelPortaliO62618.
SMRiO62618. Positions 9-356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 312288Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi184 – 1863TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074271.
InParanoidiO62618.
KOiK04441.
OMAiLNLIMEM.
OrthoDBiEOG7PCJGV.
PhylomeDBiO62618.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O62618-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVSITKKFY KLDINRTEWE IPDIYQDLQP VGSGAYGQVS KAVVRGTNMH
60 70 80 90 100
VAIKKLARPF QSAVHAKRTY RELRLLKHMD HENVIGLLDI FHPHPANGSL
110 120 130 140 150
ENFQQVYLVT HLMDADLNNI IRMQHLSDDH VQFLVYQILR GLKYIHSAGV
160 170 180 190 200
IHRDLKPSNI AVNEDCELRI LDFGLARPTE NEMTGYVATR WYRAPEIMLN
210 220 230 240 250
WMHYDQTVDI WSVGCIMAEL ITRRTLFPGT DHIHQLNLIM EMLGTPPAEF
260 270 280 290 300
LKKISSESAR SYIQSLPPMK GRSFKNVFKN ANPLAIDLLE KMLELDAEKR
310 320 330 340 350
ITAEEALSHP YLEKYAEPSV EQTSPPYDHS FEDMDLPVDK WKELIYKEVT
360
NFKPPPSYAQ VLKDVK
Length:366
Mass (Da):42,256
Last modified:August 1, 1998 - v1
Checksum:iB3592B869F97990E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 271D → G in AAB97138. (PubMed:9584193)Curated
Sequence conflicti77 – 771K → R(PubMed:1378625)Curated
Sequence conflicti80 – 801D → A in AAB97138. (PubMed:9584193)Curated
Sequence conflicti108 – 1081L → LL(PubMed:1378625)Curated
Sequence conflicti124 – 1241Q → QQ(PubMed:1378625)Curated
Sequence conflicti149 – 1491Missing(PubMed:1378625)Curated
Sequence conflicti163 – 1631N → NN(PubMed:1378625)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86867 mRNA. Translation: AAB97138.1.
AF035546 Genomic DNA. Translation: AAC39030.1.
AF035547 mRNA. Translation: AAC39031.1.
AE014297 Genomic DNA. Translation: AAF56244.1.
AE014297 Genomic DNA. Translation: AAN13984.1.
AY071670 mRNA. Translation: AAL49292.1.
RefSeqiNP_001163711.1. NM_001170240.2.
NP_477163.1. NM_057815.5.
NP_732959.1. NM_170126.5.
UniGeneiDm.2996.

Genome annotation databases

EnsemblMetazoaiFBtr0084580; FBpp0083965; FBgn0015765.
FBtr0084581; FBpp0083966; FBgn0015765.
FBtr0300572; FBpp0289799; FBgn0015765.
GeneIDi42866.
KEGGidme:Dmel_CG5475.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86867 mRNA. Translation: AAB97138.1 .
AF035546 Genomic DNA. Translation: AAC39030.1 .
AF035547 mRNA. Translation: AAC39031.1 .
AE014297 Genomic DNA. Translation: AAF56244.1 .
AE014297 Genomic DNA. Translation: AAN13984.1 .
AY071670 mRNA. Translation: AAL49292.1 .
RefSeqi NP_001163711.1. NM_001170240.2.
NP_477163.1. NM_057815.5.
NP_732959.1. NM_170126.5.
UniGenei Dm.2996.

3D structure databases

ProteinModelPortali O62618.
SMRi O62618. Positions 9-356.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 67799. 4 interactions.
IntActi O62618. 2 interactions.
MINTi MINT-4080391.
STRINGi 7227.FBpp0083966.

Proteomic databases

PaxDbi O62618.
PRIDEi O62618.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0084580 ; FBpp0083965 ; FBgn0015765 .
FBtr0084581 ; FBpp0083966 ; FBgn0015765 .
FBtr0300572 ; FBpp0289799 ; FBgn0015765 .
GeneIDi 42866.
KEGGi dme:Dmel_CG5475.

Organism-specific databases

CTDi 42866.
FlyBasei FBgn0015765. Mpk2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074271.
InParanoidi O62618.
KOi K04441.
OMAi LNLIMEM.
OrthoDBi EOG7PCJGV.
PhylomeDBi O62618.

Enzyme and pathway databases

BRENDAi 2.7.11.24. 1994.
Reactomei REACT_180302. KSRP destabilizes mRNA.
REACT_181712. Oxidative Stress Induced Senescence.
REACT_203135. Activation of PPARGC1A (PGC-1alpha) by phosphorylation.
REACT_221889. Platelet sensitization by LDL.
REACT_246045. CDO in myogenesis.
REACT_246078. p38MAPK events.
REACT_249521. NOD1/2 Signaling Pathway.
REACT_252605. VEGFA-VEGFR2 Pathway.
REACT_255277. ADP signalling through P2Y purinoceptor 1.
REACT_257192. activated TAK1 mediates p38 MAPK activation.
REACT_263734. ERK/MAPK targets.
REACT_34513. DSCAM interactions.
SignaLinki O62618.

Miscellaneous databases

GenomeRNAii 42866.
NextBioi 830992.

Gene expression databases

Bgeei O62618.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01773. P38MAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a Drosophila p38 mitogen-activated protein kinase."
    Han S.-J., Choi K.-Y., Brey P.T., Lee W.-J.
    J. Biol. Chem. 273:369-374(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION AT TYR-186, FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION.
    Tissue: Hemocyte.
  2. "A conserved p38 mitogen-activated protein kinase pathway regulates Drosophila immunity gene expression."
    Han Z.S., Enslen H., Hu X., Meng X., Wu I.-H., Barrett T., Davis R.J., Ip Y.T.
    Mol. Cell. Biol. 18:3527-3539(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DEVELOPMENTAL STAGE, ENZYME REGULATION.
    Tissue: Embryo.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Head.
  6. "Primary structure, expression, and signal-dependent tyrosine phosphorylation of a Drosophila homolog of extracellular signal-regulated kinase."
    Biggs W.H. III, Zipursky S.L.
    Proc. Natl. Acad. Sci. U.S.A. 89:6295-6299(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 38-188.
    Tissue: Imaginal disk.

Entry informationi

Entry nameiMK14A_DROME
AccessioniPrimary (citable) accession number: O62618
Secondary accession number(s): A4V3C0, O46216, Q9TXB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: August 1, 1998
Last modified: November 26, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3