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Protein

Serine protease gd

Gene

gd

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the extracellular signaling pathway that establishes the dorsal-ventral pathway of the embryo (PubMed:9618496, PubMed:9477324, PubMed:12493753). Three proteases; ndl, gd and snk process easter to create active easter (PubMed:9477324). Active easter defines cell identities along the dorsal-ventral continuum by activating the spz ligand for the Tl receptor in the ventral region of the embryo (PubMed:9477324).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei295Charge relay systemPROSITE-ProRule annotation1
Active sitei350Charge relay systemPROSITE-ProRule annotation1
Active sitei471Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

  • peptidase activity Source: FlyBase
  • serine-type endopeptidase activity Source: FlyBase
  • serine-type peptidase activity Source: FlyBase

GO - Biological processi

  • dorsal/ventral axis specification Source: FlyBase
  • dorsal/ventral pattern formation Source: UniProtKB
  • maternal specification of dorsal/ventral axis, oocyte, germ-line encoded Source: FlyBase
  • protein processing Source: FlyBase
  • proteolysis Source: FlyBase
  • Toll signaling pathway Source: FlyBase
  • ventral furrow formation Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-DME-209442 Formation of the trans-membrane 'signalling complex'

Protein family/group databases

MEROPSiS01.202

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease gd (EC:3.4.21.-)
Alternative name(s):
Protein gastrulation defective
Gene namesi
Name:gd
ORF Names:CG1505
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000808 gd

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi513Y → A or P: Results in a defective gastrulation with excessive ventralization showing very prominent ventral furrow, no anterior displacement of posterior cells and only a small headfold on the dorsal side of the embryo. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000002813520 – 531Serine protease gdAdd BLAST512

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi272N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi280 ↔ 296PROSITE-ProRule annotation
Glycosylationi397N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi432 ↔ 449PROSITE-ProRule annotation
Glycosylationi445N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO62589
PRIDEiO62589

Expressioni

Tissue specificityi

Expression begins in previtellogenic stages and is seen in germline-derived nurse cells of the germarium. Expression continues throughout oogenesis with transcripts from the nurse cells accumulating in the oocytes. Most abundant in the ovaries, the level of protein decreases from the moment of egg laying and is essentially gone by 4 hours.1 Publication

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0000808
ExpressionAtlasiO62589 baseline and differential
GenevisibleiO62589 DM

Interactioni

Protein-protein interaction databases

BioGridi58564, 6 interactors
IntActiO62589, 1 interactor
STRINGi7227.FBpp0304588

Structurei

3D structure databases

ProteinModelPortaliO62589
SMRiO62589
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini246 – 531Peptidase S1PROSITE-ProRule annotationAdd BLAST286

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00530000063959
InParanoidiO62589
OMAiNQVICFG
OrthoDBiEOG091G069Q

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR031986 GD_N
IPR009003 Peptidase_S1_PA
IPR001254 Trypsin_dom
PfamiView protein in Pfam
PF16030 GD_N, 1 hit
PF00089 Trypsin, 1 hit
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform DImported (identifier: O62589-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLHLAAILI LCIEHVTKAV AQGMPISPCP KVFQYRFDGS EWFGLMAVRS
60 70 80 90 100
PDGHQPLHIR VTLSMRGKPT TYTQNYLGEI ELLTRGKFTH NAPVLYKIRF
110 120 130 140 150
PKHHFPPKLL LMSANNHVIC FGSGEHSIFM TQIQLEHIRK LSFIPDKKSS
160 170 180 190 200
LLLDPEEEEV RKTDDKPPST PHIQFKKKPF AQAPKEICGR IDRDLDFHLS
210 220 230 240 250
QRTESLHVAI GEPKSSDGIT SPVFVDDDED DVLEHQFVDE SEAEAIESDS
260 270 280 290 300
ADSLPSITRG SWPWLAAIYV NNLTSLDFQC GGSLVSARVV ISSAHCFKLF
310 320 330 340 350
NKRYTSNEVL VFLGRHNLKN WNEEGSLAAP VDGIYIHPDF NSQLSSYDAD
360 370 380 390 400
IAVIILKDEV RFNTFIRPAC LWSGSSKTEY IVGERGIVIG WSFDRTNRTR
410 420 430 440 450
DQKLSSELPG KKSTDASAPK VVKAPIVGNA ECFRANAHFR SLSSNRTFCA
460 470 480 490 500
GIQAEERDTH QSGASIYTGI SGAGLFIRRN NRWMLRGTVS AALPAVETPD
510 520 530
AESSHKLCCK NQYIIYADVA KFLDWITAFV I
Note: No experimental confirmation available.
Length:531
Mass (Da):59,477
Last modified:May 14, 2014 - v2
Checksum:iD420F2879DB92C97
GO
Isoform CImported (identifier: O62589-2) [UniParc]FASTAAdd to basket
Also known as: FImported

The sequence of this isoform differs from the canonical sequence as follows:
     72-74: Missing.

Show »
Length:528
Mass (Da):59,084
Checksum:i31E3BEBBC5AE07A8
GO
Isoform EImported (identifier: O62589-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     72-74: Missing.
     178-182: KPFAQ → YASGF
     183-531: Missing.

Note: No experimental confirmation available.
Show »
Length:179
Mass (Da):20,453
Checksum:i3E2FFF47DDF03F4A
GO

Sequence cautioni

The sequence ABG02140 differs from that shown. Intron retention.Curated
The sequence ABG02140 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence ADK93997 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05445172 – 74Missing in isoform C and isoform E. 2 Publications3
Alternative sequenceiVSP_054452178 – 182KPFAQ → YASGF in isoform E. Curated5
Alternative sequenceiVSP_054453183 – 531Missing in isoform E. CuratedAdd BLAST349

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056311 mRNA Translation: AAC13558.1
U09808 Genomic DNA Translation: AAC24235.1
AE014298 Genomic DNA Translation: AAF48122.3
AE014298 Genomic DNA Translation: AGB95321.1
AE014298 Genomic DNA Translation: AGB95322.1
AE014298 Genomic DNA Translation: AGB95323.1
BT025896 mRNA Translation: ABG02140.1 Sequence problems.
BT125069 mRNA Translation: ADK93997.1 Different initiation.
RefSeqiNP_001259478.1, NM_001272549.1 [O62589-2]
NP_001259479.1, NM_001272550.1 [O62589-1]
NP_001259480.1, NM_001272551.1 [O62589-3]
NP_001303552.1, NM_001316623.1 [O62589-2]
UniGeneiDm.2923

Genome annotation databases

EnsemblMetazoaiFBtr0332308; FBpp0304587; FBgn0000808 [O62589-2]
FBtr0332309; FBpp0304588; FBgn0000808 [O62589-1]
FBtr0332310; FBpp0304589; FBgn0000808 [O62589-3]
FBtr0347416; FBpp0312566; FBgn0000808 [O62589-2]
GeneIDi32159
KEGGidme:Dmel_CG1505

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGD_DROME
AccessioniPrimary (citable) accession number: O62589
Secondary accession number(s): D9PTR2
, M9PEE0, M9PGY7, M9PJK1, Q1EC38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 14, 2014
Last modified: May 23, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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