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Protein

Serine protease gd

Gene

gd

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the extracellular signaling pathway that establishes the dorsal-ventral pathway of the embryo. Three proteases; ndl, gd and snk process easter to create active easter. Active easter defines cell identities along the dorsal-ventral continuum by activating the spz ligand for the Tl receptor in the ventral region of the embryo.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei295 – 2951Charge relay systemPROSITE-ProRule annotation
Active sitei350 – 3501Charge relay systemPROSITE-ProRule annotation
Active sitei471 – 4711Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

  • peptidase activity Source: FlyBase
  • serine-type endopeptidase activity Source: FlyBase
  • serine-type peptidase activity Source: FlyBase

GO - Biological processi

  • dorsal/ventral axis specification Source: FlyBase
  • maternal specification of dorsal/ventral axis, oocyte, germ-line encoded Source: FlyBase
  • protein processing Source: FlyBase
  • proteolysis Source: FlyBase
  • Toll signaling pathway Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.202.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease gd (EC:3.4.21.-)
Alternative name(s):
Protein gastrulation defective
Gene namesi
Name:gd
ORF Names:CG1505
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000808. gd.

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

  • extracellular region Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 531512Serine protease gdPRO_0000028135Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence analysis
Disulfide bondi280 ↔ 296PROSITE-ProRule annotation
Glycosylationi397 – 3971N-linked (GlcNAc...)Sequence analysis
Disulfide bondi432 ↔ 449PROSITE-ProRule annotation
Glycosylationi445 – 4451N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO62589.
PRIDEiO62589.

Expressioni

Tissue specificityi

Expression begins in previtellogenic stages and is seen in germline-derived nurse cells of the germarium. Expression continues throughout oogenesis with transcripts from the nurse cells accumulating in the oocytes. Most abundant in the ovaries, the level of protein decreases from the moment of egg laying and is essentially gone by 4 hours.1 Publication

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiO62589.
ExpressionAtlasiO62589. differential.
GenevisibleiO62589. DM.

Interactioni

Protein-protein interaction databases

BioGridi58564. 4 interactions.
IntActiO62589. 1 interaction.
STRINGi7227.FBpp0304588.

Structurei

3D structure databases

ProteinModelPortaliO62589.
SMRiO62589. Positions 222-530.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini246 – 531286Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00530000063959.
InParanoidiO62589.
OMAiMTQIQLE.
OrthoDBiEOG7TMZRW.

Family and domain databases

InterProiIPR031986. GD_N.
IPR009003. Peptidase_S1_PA.
IPR001254. Trypsin_dom.
[Graphical view]
PfamiPF16030. GD_N. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform DImported (identifier: O62589-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLHLAAILI LCIEHVTKAV AQGMPISPCP KVFQYRFDGS EWFGLMAVRS
60 70 80 90 100
PDGHQPLHIR VTLSMRGKPT TYTQNYLGEI ELLTRGKFTH NAPVLYKIRF
110 120 130 140 150
PKHHFPPKLL LMSANNHVIC FGSGEHSIFM TQIQLEHIRK LSFIPDKKSS
160 170 180 190 200
LLLDPEEEEV RKTDDKPPST PHIQFKKKPF AQAPKEICGR IDRDLDFHLS
210 220 230 240 250
QRTESLHVAI GEPKSSDGIT SPVFVDDDED DVLEHQFVDE SEAEAIESDS
260 270 280 290 300
ADSLPSITRG SWPWLAAIYV NNLTSLDFQC GGSLVSARVV ISSAHCFKLF
310 320 330 340 350
NKRYTSNEVL VFLGRHNLKN WNEEGSLAAP VDGIYIHPDF NSQLSSYDAD
360 370 380 390 400
IAVIILKDEV RFNTFIRPAC LWSGSSKTEY IVGERGIVIG WSFDRTNRTR
410 420 430 440 450
DQKLSSELPG KKSTDASAPK VVKAPIVGNA ECFRANAHFR SLSSNRTFCA
460 470 480 490 500
GIQAEERDTH QSGASIYTGI SGAGLFIRRN NRWMLRGTVS AALPAVETPD
510 520 530
AESSHKLCCK NQYIIYADVA KFLDWITAFV I
Note: No experimental confirmation available.
Length:531
Mass (Da):59,477
Last modified:May 14, 2014 - v2
Checksum:iD420F2879DB92C97
GO
Isoform CImported (identifier: O62589-2) [UniParc]FASTAAdd to basket

Also known as: FImported

The sequence of this isoform differs from the canonical sequence as follows:
     72-74: Missing.

Show »
Length:528
Mass (Da):59,084
Checksum:i31E3BEBBC5AE07A8
GO
Isoform EImported (identifier: O62589-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     72-74: Missing.
     178-182: KPFAQ → YASGF
     183-531: Missing.

Note: No experimental confirmation available.
Show »
Length:179
Mass (Da):20,453
Checksum:i3E2FFF47DDF03F4A
GO

Sequence cautioni

The sequence ABG02140.1 differs from that shown.Intron retention.Curated
The sequence ABG02140.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence ADK93997.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei72 – 743Missing in isoform C and isoform E. 2 PublicationsVSP_054451
Alternative sequencei178 – 1825KPFAQ → YASGF in isoform E. CuratedVSP_054452
Alternative sequencei183 – 531349Missing in isoform E. CuratedVSP_054453Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056311 mRNA. Translation: AAC13558.1.
U09808 Genomic DNA. Translation: AAC24235.1.
AE014298 Genomic DNA. Translation: AAF48122.3.
AE014298 Genomic DNA. Translation: AGB95321.1.
AE014298 Genomic DNA. Translation: AGB95322.1.
AE014298 Genomic DNA. Translation: AGB95323.1.
BT025896 mRNA. Translation: ABG02140.1. Sequence problems.
BT125069 mRNA. Translation: ADK93997.1. Different initiation.
RefSeqiNP_001259478.1. NM_001272549.1. [O62589-2]
NP_001259479.1. NM_001272550.1. [O62589-1]
NP_001259480.1. NM_001272551.1. [O62589-3]
NP_001303552.1. NM_001316623.1. [O62589-2]
UniGeneiDm.2923.

Genome annotation databases

EnsemblMetazoaiFBtr0332309; FBpp0304588; FBgn0000808. [O62589-1]
GeneIDi32159.
KEGGidme:Dmel_CG1505.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056311 mRNA. Translation: AAC13558.1.
U09808 Genomic DNA. Translation: AAC24235.1.
AE014298 Genomic DNA. Translation: AAF48122.3.
AE014298 Genomic DNA. Translation: AGB95321.1.
AE014298 Genomic DNA. Translation: AGB95322.1.
AE014298 Genomic DNA. Translation: AGB95323.1.
BT025896 mRNA. Translation: ABG02140.1. Sequence problems.
BT125069 mRNA. Translation: ADK93997.1. Different initiation.
RefSeqiNP_001259478.1. NM_001272549.1. [O62589-2]
NP_001259479.1. NM_001272550.1. [O62589-1]
NP_001259480.1. NM_001272551.1. [O62589-3]
NP_001303552.1. NM_001316623.1. [O62589-2]
UniGeneiDm.2923.

3D structure databases

ProteinModelPortaliO62589.
SMRiO62589. Positions 222-530.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58564. 4 interactions.
IntActiO62589. 1 interaction.
STRINGi7227.FBpp0304588.

Protein family/group databases

MEROPSiS01.202.

Proteomic databases

PaxDbiO62589.
PRIDEiO62589.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0332309; FBpp0304588; FBgn0000808. [O62589-1]
GeneIDi32159.
KEGGidme:Dmel_CG1505.

Organism-specific databases

CTDi619206.
FlyBaseiFBgn0000808. gd.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00530000063959.
InParanoidiO62589.
OMAiMTQIQLE.
OrthoDBiEOG7TMZRW.

Miscellaneous databases

GenomeRNAii32159.
NextBioi777176.
PROiO62589.

Gene expression databases

BgeeiO62589.
ExpressionAtlasiO62589. differential.
GenevisibleiO62589. DM.

Family and domain databases

InterProiIPR031986. GD_N.
IPR009003. Peptidase_S1_PA.
IPR001254. Trypsin_dom.
[Graphical view]
PfamiPF16030. GD_N. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The gastrulation defective gene of Drosophila melanogaster is a member of the serine protease superfamily."
    Konrad K.D., Goralski T.J., Mahowald A.P., Marsh J.L.
    Proc. Natl. Acad. Sci. U.S.A. 95:6819-6824(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM C), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Embryo.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Stapleton M., Booth B., Carlson J., Chavez C., Frise E., George R., Pacleb J., Park S., Wan K., Yu C., Celniker S.
    Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
    Strain: Berkeley.
  5. "Positive and negative regulation of Easter, a member of the serine protease family that controls dorsal-ventral patterning in the Drosophila embryo."
    Misra S., Hecht P., Maeda R., Anderson K.V.
    Development 125:1261-1267(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF EASTER, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGD_DROME
AccessioniPrimary (citable) accession number: O62589
Secondary accession number(s): D9PTR2
, M9PEE0, M9PGY7, M9PJK1, Q1EC38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 14, 2014
Last modified: May 11, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.