Reviewed, UniProtKB/Swiss-Prot O62584 (TYS1_ENCCU)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase 1/2
      Short name=TS 1/2
      Short name=TSase 1/2
    EC=2.1.1.45

Gene names

Name:	TS-1
Ordered Locus Names:	ECU01_0180
AND
Name:	TS-2
Ordered Locus Names:	ECU01_1430

Organism

Encephalitozoon cuniculi (Microsporidian parasite) [Complete proteome]

Taxonomic identifier

6035 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Microsporidia › Unikaryonidae › Encephalitozoon

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.
Pathway	Pyrimidine metabolism; dTTP biosynthesis.
Sequence similarities	Belongs to the thymidylate synthase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Thymidylate synthase 1/2		PRO_0000140906
Sites
	Active site	174	1	By similarity
Experimental info
	Sequence conflict	34	1	T → A in CAA06648. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O62584-1 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: 5FF3C707E86C0C56	FASTA	294	33,112
	10 20 30 40 50 60 MPQDPRHPEH QYLDLVKHIL ENGARRMDRT GTGTLSVFGA TMRFSLEDNT FPLLTTRRVF 70 80 90 100 110 120 YRGVVEELLF FLRGETDSKV LEKKGVRIWE KNGAKQFLQS VGIDREEGDL GPIYGFQWRH 130 140 150 160 170 180 FGARYETSAS SYEGKGVDQI ASAIAAIRAN PASRRIVVSA WNPTDLGSMA LPPCHVLFQF 190 200 210 220 230 240 NVTDGKLSCA MYQRSGDMGL GVPFNIASYS LLTILVAHLT GLQPGEFVHF LGDAHVYLDH 250 260 270 280 290 VDSLRQQIQR PPRAFPKLFV SPKGPRTEPE HFQYEDFELV GYDPHPAIKM NMSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"First report on the systematic sequencing of the small genome of Encephalitozoon cuniculi (Protozoa, Microspora): gene organization of a 4.3 kbp region on chromosome I." Duffieux F., Peyret P., Roe B.A., Vivares C.P. Microb. Comp. Genomics 3:1-11(1998) [PubMed: 11013707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of chromosome I of the amitochondriate intracellular parasite Encephalitozoon cuniculi (Microspora)." Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M., Weissenbach J., Saurin W., Vivares C.P. Genome Res. 11:198-207(2001) [PubMed: 11157783] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.
[3]	"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed: 11719806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ005644 Genomic DNA. Translation: CAA06648.1. AL391737 Genomic DNA. Translation: CAD24888.1. AL391737 Genomic DNA. Translation: CAD25016.1.
RefSeq	XP_965853.1. XP_965981.1.
3D structure databases
HSSP	HSSP built from PDB template 1RTS based on UniProtKB P45352.
ModBase	Search...
Genome annotation databases
GeneID	860190. 860191.
GenomeReviews	Gene locus ECU01_0180 in contig AL391737_GR. Gene locus ECU01_1430 in contig AL391737_GR.
KEGG	ecu:ECU01_0180. ecu:ECU01_1430.
Phylogenomic databases
HOGENOM	O62584.
Enzyme and pathway databases
BRENDA	2.1.1.45. 276319.
Family and domain databases
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYS1_ENCCU

Accession

Primary (citable) accession number: O62584
Secondary accession number(s): Q8SQI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 31, 2004
Last modified:	September 22, 2009
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)