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Protein

Thymidylate synthase 1/2

Gene

TS-1

more
Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei29dUMPBy similarity1
Active sitei174NucleophileBy similarity1
Binding sitei1975,10-methylenetetrahydrofolateBy similarity1
Binding sitei205dUMPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi154 – 155dUMP; shared with dimeric partnerBy similarity2
Nucleotide bindingi194 – 197dUMPBy similarity4
Nucleotide bindingi235 – 237dUMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase 1/2 (EC:2.1.1.45)
Short name:
TS 1/2
Short name:
TSase 1/2
Gene namesi
Name:TS-1
Ordered Locus Names:ECU01_0180
AND
Name:TS-2
Ordered Locus Names:ECU01_1430
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMicrosporidiaUnikaryonidaeEncephalitozoon
Proteomesi
  • UP000000819 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409061 – 294Thymidylate synthase 1/2Add BLAST294

Interactioni

Protein-protein interaction databases

STRINGi284813.XP_965981.1

Structurei

Secondary structure

1294
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 22Combined sources14
Beta strandi24 – 26Combined sources3
Beta strandi34 – 45Combined sources12
Helixi47 – 49Combined sources3
Beta strandi55 – 57Combined sources3
Helixi61 – 72Combined sources12
Helixi79 – 82Combined sources4
Turni83 – 85Combined sources3
Helixi114 – 119Combined sources6
Beta strandi135 – 137Combined sources3
Helixi139 – 149Combined sources11
Beta strandi157 – 159Combined sources3
Helixi166 – 170Combined sources5
Beta strandi174 – 183Combined sources10
Beta strandi186 – 197Combined sources12
Turni198 – 200Combined sources3
Helixi201 – 219Combined sources19
Beta strandi223 – 237Combined sources15
Helixi238 – 240Combined sources3
Helixi241 – 247Combined sources7
Beta strandi257 – 260Combined sources4
Helixi269 – 271Combined sources3
Helixi274 – 276Combined sources3
Beta strandi277 – 281Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KGBX-ray2.20A1-294[»]
ProteinModelPortaliO62584
SMRiO62584
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO62584

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

eggNOGiKOG0673 Eukaryota
COG0207 LUCA
HOGENOMiHOG000257899
InParanoidiO62584
KOiK00560
OMAiKQYLDLC
OrthoDBiEOG092C3QQ6

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

O62584-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQDPRHPEH QYLDLVKHIL ENGARRMDRT GTGTLSVFGA TMRFSLEDNT
60 70 80 90 100
FPLLTTRRVF YRGVVEELLF FLRGETDSKV LEKKGVRIWE KNGAKQFLQS
110 120 130 140 150
VGIDREEGDL GPIYGFQWRH FGARYETSAS SYEGKGVDQI ASAIAAIRAN
160 170 180 190 200
PASRRIVVSA WNPTDLGSMA LPPCHVLFQF NVTDGKLSCA MYQRSGDMGL
210 220 230 240 250
GVPFNIASYS LLTILVAHLT GLQPGEFVHF LGDAHVYLDH VDSLRQQIQR
260 270 280 290
PPRAFPKLFV SPKGPRTEPE HFQYEDFELV GYDPHPAIKM NMSA
Length:294
Mass (Da):33,112
Last modified:August 31, 2004 - v2
Checksum:i5FF3C707E86C0C56
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34T → A in CAA06648 (PubMed:11157783).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005644 Genomic DNA Translation: CAA06648.1
AL391737 Genomic DNA Translation: CAD24888.1
AL391737 Genomic DNA Translation: CAD25016.1
RefSeqiXP_965853.1, XM_960760.1
XP_965981.1, XM_960888.1

Genome annotation databases

EnsemblFungiiCAD24888; CAD24888; CAD24888
CAD25016; CAD25016; CAD25016
GeneIDi860190
860191
KEGGiecu:ECU01_0180
ecu:ECU01_1430

Similar proteinsi

Entry informationi

Entry nameiTYS1_ENCCU
AccessioniPrimary (citable) accession number: O62584
Secondary accession number(s): Q8SQI4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 31, 2004
Last modified: May 23, 2018
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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