O62584 (TYS1_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase 1/2
Short name=TS 1/2
Short name=TSase 1/2
EC=2.1.1.45

Gene names

Name:	TS-1
Ordered Locus Names:	ECU01_0180
AND
Name:	TS-2
Ordered Locus Names:	ECU01_1430

Organism

Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]

Taxonomic identifier

284813 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Unikaryonidae › Encephalitozoon › Encephalitozoon cuniculi

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.
Pathway	Pyrimidine metabolism; dTTP biosynthesis.
Sequence similarities	Belongs to the thymidylate synthase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 294

294

Thymidylate synthase 1/2

PRO_0000140906

Sites

Active site

174

1

By similarity

Experimental info

Sequence conflict

34

1

T → A in CAA06648. Ref.2

Secondary structure

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294

Helix Strand Turn

Details...

Helix

9 – 22

14

Beta strand

24 – 26

3

Beta strand

34 – 45

12

Helix

47 – 49

3

Beta strand

55 – 57

3

Helix

61 – 72

12

Helix

79 – 82

4

Turn

83 – 85

3

Helix

114 – 119

6

Beta strand

135 – 137

3

Helix

139 – 149

11

Beta strand

157 – 159

3

Helix

166 – 170

5

Beta strand

174 – 183

10

Beta strand

186 – 197

12

Turn

198 – 200

3

Helix

201 – 219

19

Beta strand

223 – 237

15

Helix

238 – 240

3

Helix

241 – 247

7

Beta strand

257 – 260

4

Helix

269 – 271

3

Helix

274 – 276

3

Beta strand

277 – 281

5

Sequences

	Sequence		Length	Mass (Da)
	O62584 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: 5FF3C707E86C0C56	FASTA	294	33,112
	10 20 30 40 50 60 MPQDPRHPEH QYLDLVKHIL ENGARRMDRT GTGTLSVFGA TMRFSLEDNT FPLLTTRRVF 70 80 90 100 110 120 YRGVVEELLF FLRGETDSKV LEKKGVRIWE KNGAKQFLQS VGIDREEGDL GPIYGFQWRH 130 140 150 160 170 180 FGARYETSAS SYEGKGVDQI ASAIAAIRAN PASRRIVVSA WNPTDLGSMA LPPCHVLFQF 190 200 210 220 230 240 NVTDGKLSCA MYQRSGDMGL GVPFNIASYS LLTILVAHLT GLQPGEFVHF LGDAHVYLDH 250 260 270 280 290 VDSLRQQIQR PPRAFPKLFV SPKGPRTEPE HFQYEDFELV GYDPHPAIKM NMSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"First report on the systematic sequencing of the small genome of Encephalitozoon cuniculi (Protozoa, Microspora): gene organization of a 4.3 kbp region on chromosome I." Duffieux F., Peyret P., Roe B.A., Vivares C.P. Microb. Comp. Genomics 3:1-11(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of chromosome I of the amitochondriate intracellular parasite Encephalitozoon cuniculi (Microspora)." Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M., Weissenbach J., Saurin W., Vivares C.P. Genome Res. 11:198-207(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.
[3]	"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ005644 Genomic DNA. Translation: CAA06648.1.
AL391737 Genomic DNA. Translation: CAD24888.1.
AL391737 Genomic DNA. Translation: CAD25016.1.

RefSeq

XP_965853.1. XM_960760.1.
XP_965981.1. XM_960888.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3KGB	X-ray	2.20	A	1-294	[»]

ProteinModelPortal

O62584.

SMR

O62584. Positions 6-290.

ModBase

Search...

Protein-protein interaction databases

STRING

6035.ECU01_1430.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

860190.
860191.

KEGG

ecu:ECU01_0180.
ecu:ECU01_1430.

Phylogenomic databases

eggNOG

COG0207.

HOGENOM

HOG000257899.

KO

K00560.

OMA

DTNVAYL.

Enzyme and pathway databases

UniPathway

UPA00575.

Family and domain databases

Gene3D

3.30.572.10. 1 hit.

InterPro

IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]

Pfam

PF00303. Thymidylat_synt. 1 hit.
[Graphical view]

PRINTS

PR00108. THYMDSNTHASE.

SUPFAM

SSF55831. Thymidylat_synth_C. 1 hit.

TIGRFAMs

TIGR03284. thym_sym. 1 hit.

PROSITE

PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O62584.

Entry information

Entry name

TYS1_ENCCU

Accession

Primary (citable) accession number: O62584
Secondary accession number(s): Q8SQI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 31, 2004
Last modified:	May 1, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program