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O62584

- TYS1_ENCCU

UniProt

O62584 - TYS1_ENCCU

Protein

Thymidylate synthase 1/2

Gene

TS-1

more
Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 2 (31 Aug 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei174 – 1741By similarity

    GO - Molecular functioni

    1. thymidylate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. dTMP biosynthetic process Source: InterPro
    2. dTTP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate synthase 1/2 (EC:2.1.1.45)
    Short name:
    TS 1/2
    Short name:
    TSase 1/2
    Gene namesi
    Name:TS-1
    Ordered Locus Names:ECU01_0180
    AND
    Name:TS-2
    Ordered Locus Names:ECU01_1430
    OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
    Taxonomic identifieri284813 [NCBI]
    Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
    ProteomesiUP000000819: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 294294Thymidylate synthase 1/2PRO_0000140906Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi6035.ECU01_1430.

    Structurei

    Secondary structure

    1
    294
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 2214
    Beta strandi24 – 263
    Beta strandi34 – 4512
    Helixi47 – 493
    Beta strandi55 – 573
    Helixi61 – 7212
    Helixi79 – 824
    Turni83 – 853
    Helixi114 – 1196
    Beta strandi135 – 1373
    Helixi139 – 14911
    Beta strandi157 – 1593
    Helixi166 – 1705
    Beta strandi174 – 18310
    Beta strandi186 – 19712
    Turni198 – 2003
    Helixi201 – 21919
    Beta strandi223 – 23715
    Helixi238 – 2403
    Helixi241 – 2477
    Beta strandi257 – 2604
    Helixi269 – 2713
    Helixi274 – 2763
    Beta strandi277 – 2815

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3KGBX-ray2.20A1-294[»]
    ProteinModelPortaliO62584.
    SMRiO62584. Positions 6-290.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO62584.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG0207.
    HOGENOMiHOG000257899.
    KOiK00560.
    OMAiKWARSKE.
    OrthoDBiEOG7X6M9G.

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 1 hit.
    PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O62584-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPQDPRHPEH QYLDLVKHIL ENGARRMDRT GTGTLSVFGA TMRFSLEDNT    50
    FPLLTTRRVF YRGVVEELLF FLRGETDSKV LEKKGVRIWE KNGAKQFLQS 100
    VGIDREEGDL GPIYGFQWRH FGARYETSAS SYEGKGVDQI ASAIAAIRAN 150
    PASRRIVVSA WNPTDLGSMA LPPCHVLFQF NVTDGKLSCA MYQRSGDMGL 200
    GVPFNIASYS LLTILVAHLT GLQPGEFVHF LGDAHVYLDH VDSLRQQIQR 250
    PPRAFPKLFV SPKGPRTEPE HFQYEDFELV GYDPHPAIKM NMSA 294
    Length:294
    Mass (Da):33,112
    Last modified:August 31, 2004 - v2
    Checksum:i5FF3C707E86C0C56
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti34 – 341T → A in CAA06648. (PubMed:11157783)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005644 Genomic DNA. Translation: CAA06648.1.
    AL391737 Genomic DNA. Translation: CAD24888.1.
    AL391737 Genomic DNA. Translation: CAD25016.1.
    RefSeqiXP_965853.1. XM_960760.1.
    XP_965981.1. XM_960888.1.

    Genome annotation databases

    GeneIDi860190.
    860191.
    KEGGiecu:ECU01_0180.
    ecu:ECU01_1430.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005644 Genomic DNA. Translation: CAA06648.1 .
    AL391737 Genomic DNA. Translation: CAD24888.1 .
    AL391737 Genomic DNA. Translation: CAD25016.1 .
    RefSeqi XP_965853.1. XM_960760.1.
    XP_965981.1. XM_960888.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3KGB X-ray 2.20 A 1-294 [» ]
    ProteinModelPortali O62584.
    SMRi O62584. Positions 6-290.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6035.ECU01_1430.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 860190.
    860191.
    KEGGi ecu:ECU01_0180.
    ecu:ECU01_1430.

    Phylogenomic databases

    eggNOGi COG0207.
    HOGENOMi HOG000257899.
    KOi K00560.
    OMAi KWARSKE.
    OrthoDBi EOG7X6M9G.

    Enzyme and pathway databases

    UniPathwayi UPA00575 .

    Miscellaneous databases

    EvolutionaryTracei O62584.

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 1 hit.
    PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "First report on the systematic sequencing of the small genome of Encephalitozoon cuniculi (Protozoa, Microspora): gene organization of a 4.3 kbp region on chromosome I."
      Duffieux F., Peyret P., Roe B.A., Vivares C.P.
      Microb. Comp. Genomics 3:1-11(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence and analysis of chromosome I of the amitochondriate intracellular parasite Encephalitozoon cuniculi (Microspora)."
      Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M., Weissenbach J., Saurin W., Vivares C.P.
      Genome Res. 11:198-207(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GB-M1.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GB-M1.

    Entry informationi

    Entry nameiTYS1_ENCCU
    AccessioniPrimary (citable) accession number: O62584
    Secondary accession number(s): Q8SQI4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 31, 2004
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3