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Protein

Thymidylate synthase 1/2

Gene

TS-1

more
Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei29dUMPBy similarity1
Active sitei174NucleophileBy similarity1
Binding sitei1975,10-methylenetetrahydrofolateBy similarity1
Binding sitei205dUMPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi154 – 155dUMP; shared with dimeric partnerBy similarity2
Nucleotide bindingi194 – 197dUMPBy similarity4
Nucleotide bindingi235 – 237dUMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase 1/2 (EC:2.1.1.45)
Short name:
TS 1/2
Short name:
TSase 1/2
Gene namesi
Name:TS-1
Ordered Locus Names:ECU01_0180
AND
Name:TS-2
Ordered Locus Names:ECU01_1430
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
Proteomesi
  • UP000000819 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409061 – 294Thymidylate synthase 1/2Add BLAST294

Interactioni

Protein-protein interaction databases

STRINGi284813.XP_965981.1.

Structurei

Secondary structure

1294
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 22Combined sources14
Beta strandi24 – 26Combined sources3
Beta strandi34 – 45Combined sources12
Helixi47 – 49Combined sources3
Beta strandi55 – 57Combined sources3
Helixi61 – 72Combined sources12
Helixi79 – 82Combined sources4
Turni83 – 85Combined sources3
Helixi114 – 119Combined sources6
Beta strandi135 – 137Combined sources3
Helixi139 – 149Combined sources11
Beta strandi157 – 159Combined sources3
Helixi166 – 170Combined sources5
Beta strandi174 – 183Combined sources10
Beta strandi186 – 197Combined sources12
Turni198 – 200Combined sources3
Helixi201 – 219Combined sources19
Beta strandi223 – 237Combined sources15
Helixi238 – 240Combined sources3
Helixi241 – 247Combined sources7
Beta strandi257 – 260Combined sources4
Helixi269 – 271Combined sources3
Helixi274 – 276Combined sources3
Beta strandi277 – 281Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KGBX-ray2.20A1-294[»]
ProteinModelPortaliO62584.
SMRiO62584.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO62584.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

eggNOGiKOG0673. Eukaryota.
COG0207. LUCA.
HOGENOMiHOG000257899.
InParanoidiO62584.
KOiK00560.
OMAiIVYELLW.
OrthoDBiEOG092C3QQ6.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O62584-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQDPRHPEH QYLDLVKHIL ENGARRMDRT GTGTLSVFGA TMRFSLEDNT
60 70 80 90 100
FPLLTTRRVF YRGVVEELLF FLRGETDSKV LEKKGVRIWE KNGAKQFLQS
110 120 130 140 150
VGIDREEGDL GPIYGFQWRH FGARYETSAS SYEGKGVDQI ASAIAAIRAN
160 170 180 190 200
PASRRIVVSA WNPTDLGSMA LPPCHVLFQF NVTDGKLSCA MYQRSGDMGL
210 220 230 240 250
GVPFNIASYS LLTILVAHLT GLQPGEFVHF LGDAHVYLDH VDSLRQQIQR
260 270 280 290
PPRAFPKLFV SPKGPRTEPE HFQYEDFELV GYDPHPAIKM NMSA
Length:294
Mass (Da):33,112
Last modified:August 31, 2004 - v2
Checksum:i5FF3C707E86C0C56
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34T → A in CAA06648 (PubMed:11157783).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005644 Genomic DNA. Translation: CAA06648.1.
AL391737 Genomic DNA. Translation: CAD24888.1.
AL391737 Genomic DNA. Translation: CAD25016.1.
RefSeqiXP_965853.1. XM_960760.1.
XP_965981.1. XM_960888.1.

Genome annotation databases

EnsemblFungiiCAD24888; CAD24888; CAD24888.
CAD25016; CAD25016; CAD25016.
GeneIDi860190.
860191.
KEGGiecu:ECU01_0180.
ecu:ECU01_1430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005644 Genomic DNA. Translation: CAA06648.1.
AL391737 Genomic DNA. Translation: CAD24888.1.
AL391737 Genomic DNA. Translation: CAD25016.1.
RefSeqiXP_965853.1. XM_960760.1.
XP_965981.1. XM_960888.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KGBX-ray2.20A1-294[»]
ProteinModelPortaliO62584.
SMRiO62584.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284813.XP_965981.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAD24888; CAD24888; CAD24888.
CAD25016; CAD25016; CAD25016.
GeneIDi860190.
860191.
KEGGiecu:ECU01_0180.
ecu:ECU01_1430.

Phylogenomic databases

eggNOGiKOG0673. Eukaryota.
COG0207. LUCA.
HOGENOMiHOG000257899.
InParanoidiO62584.
KOiK00560.
OMAiIVYELLW.
OrthoDBiEOG092C3QQ6.

Enzyme and pathway databases

UniPathwayiUPA00575.

Miscellaneous databases

EvolutionaryTraceiO62584.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYS1_ENCCU
AccessioniPrimary (citable) accession number: O62584
Secondary accession number(s): Q8SQI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 31, 2004
Last modified: November 30, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.