ID   DYR_ENCCU               Reviewed;         205 AA.
AC   O62583;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   16-JUN-2009, entry version 54.
DE   RecName: Full=Dihydrofolate reductase;
DE            EC=1.5.1.3;
GN   Name=DHFR-1; OrderedLocusNames=ECU01_0170;
GN   and
GN   Name=DHFR-2; OrderedLocusNames=ECU01_1450;
GN   and
GN   Name=DHFR-3; OrderedLocusNames=ECU08_0080;
OS   Encephalitozoon cuniculi.
OC   Eukaryota; Fungi; Microsporidia; Unikaryonidae; Encephalitozoon.
OX   NCBI_TaxID=6035;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=20468003; PubMed=11013707;
RA   Duffieux F., Peyret P., Roe B.A., Vivares C.P.;
RT   "First report on the systematic sequencing of the small genome of
RT   Encephalitozoon cuniculi (Protozoa, Microspora): gene organization of
RT   a 4.3 kbp region on chromosome I.";
RL   Microb. Comp. Genomics 3:1-11(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   MEDLINE=21109930; PubMed=11157783; DOI=10.1101/gr.164301;
RA   Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V.,
RA   Barbazanges M., Weissenbach J., Saurin W., Vivares C.P.;
RT   "Sequence and analysis of chromosome I of the amitochondriate
RT   intracellular parasite Encephalitozoon cuniculi (Microspora).";
RL   Genome Res. 11:198-207(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   MEDLINE=21576510; PubMed=11719806; DOI=10.1038/35106579;
RA   Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA   Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA   Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M.,
RA   Weissenbach J., Vivares C.P.;
RT   "Genome sequence and gene compaction of the eukaryote parasite
RT   Encephalitozoon cuniculi.";
RL   Nature 414:450-453(2001).
CC   -!- CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-
CC       dihydrofolate + NADPH.
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis;
CC       tetrahydrofolate from dihydrofolate: step 1/1.
CC   -!- MISCELLANEOUS: The reaction catalyzed by this enzyme represents an
CC       essential step for de novo glycine and purine synthesis, DNA
CC       precursor synthesis, and for the conversion of dUMP to dTMP.
CC   -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC   -!- SIMILARITY: Contains 1 DHFR (dihydrofolate reductase) domain.
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DR   EMBL; AJ005644; CAA06647.1; -; Genomic_DNA.
DR   EMBL; AL391737; CAD24887.1; -; Genomic_DNA.
DR   EMBL; AL391737; CAD25017.1; -; Genomic_DNA.
DR   EMBL; AL590448; CAD26313.1; -; Genomic_DNA.
DR   RefSeq; NP_597137.1; -.
DR   RefSeq; XP_965852.1; -.
DR   RefSeq; XP_965982.1; -.
DR   HSSP; P13922; 1J3J.
DR   GeneID; 859559; -.
DR   GeneID; 860188; -.
DR   GeneID; 860189; -.
DR   GenomeReviews; AL391737_GR; ECU01_0170.
DR   GenomeReviews; AL391737_GR; ECU01_1450.
DR   GenomeReviews; AL590448_GR; ECU08_0080.
DR   KEGG; ecu:ECU01_0170; -.
DR   KEGG; ecu:ECU01_1450; -.
DR   KEGG; ecu:ECU08_0080; -.
DR   HOGENOM; O62583; -.
DR   BioCyc; ECUN-XXX-01:ECUN-XXX-01-000015-MON; -.
DR   BioCyc; ECUN-XXX-01:ECUN-XXX-01-000145-MON; -.
DR   BioCyc; ECUN-XXX-01:ECUN-XXX-01-001587-MON; -.
DR   BRENDA; 1.5.1.3; 276319.
DR   GO; GO:0004146; F:dihydrofolate reductase activity; IEA:EC.
DR   GO; GO:0050661; F:NADP or NADPH binding; IEA:InterPro.
DR   GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR   GO; GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro.
DR   GO; GO:0006730; P:one-carbon compound metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR012259; DHFR.
DR   InterPro; IPR001796; DHFR_reg.
DR   InterPro; IPR017925; Dihydrofolate_reductase_CS.
DR   PANTHER; PTHR11549:SF1; DHFR; 1.
DR   Pfam; PF00186; DHFR_1; 1.
DR   PRINTS; PR00070; DHFR.
DR   PROSITE; PS00075; DHFR_1; FALSE_NEG.
DR   PROSITE; PS51330; DHFR_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; NADP; One-carbon metabolism; Oxidoreductase.
FT   CHAIN         1    205       Dihydrofolate reductase.
FT                                /FTId=PRO_0000186373.
FT   DOMAIN        1    201       DHFR.
SQ   SEQUENCE   205 AA;  22883 MW;  45450EAA534487DD CRC64;
     MLALVVALAS HRGIGNANAL PWPRPLAADM AWFRTLSQSI PLISPDRIAL APSASNAVVM
     GRRTWDSIPS RFRPLANRIN VVLSRGPARS TENTFFIQTF EALDSLPLPP SSMTFVIGGR
     DVYSLALESG RPHLIFATEV FESPECDVFF PHIDWASYEK RDITRDVSRL IDRTLASAFY
     SPETATFTEN GTSFKMFLYT KPETR
//