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Protein

Dihydrofolate reductase

Gene

DHFR-1

more
Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71NADP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei78 – 781SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 197NADPBy similarity
Nucleotide bindingi62 – 643NADPBy similarity
Nucleotide bindingi84 – 863NADPBy similarity
Nucleotide bindingi118 – 1258NADPBy similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. glycine biosynthetic process Source: InterPro
  2. nucleotide biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:DHFR-1
Ordered Locus Names:ECU01_0170
AND
Name:DHFR-2
Ordered Locus Names:ECU01_1450
AND
Name:DHFR-3
Ordered Locus Names:ECU08_0080
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
ProteomesiUP000000819: Chromosome I, UP000000819: Chromosome VIII

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Dihydrofolate reductasePRO_0000186373Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi6035.ECU08_0080.

Structurei

3D structure databases

ProteinModelPortaliO62583.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 201201DHFRPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni29 – 346Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0262.
HOGENOMiHOG000040235.
InParanoidiO62583.
KOiK00287.
OMAiPARSTEN.
OrthoDBiEOG7Q8D07.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O62583-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLALVVALAS HRGIGNANAL PWPRPLAADM AWFRTLSQSI PLISPDRIAL
60 70 80 90 100
APSASNAVVM GRRTWDSIPS RFRPLANRIN VVLSRGPARS TENTFFIQTF
110 120 130 140 150
EALDSLPLPP SSMTFVIGGR DVYSLALESG RPHLIFATEV FESPECDVFF
160 170 180 190 200
PHIDWASYEK RDITRDVSRL IDRTLASAFY SPETATFTEN GTSFKMFLYT

KPETR
Length:205
Mass (Da):22,883
Last modified:August 1, 1998 - v1
Checksum:i45450EAA534487DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005644 Genomic DNA. Translation: CAA06647.1.
AL391737 Genomic DNA. Translation: CAD24887.1.
AL391737 Genomic DNA. Translation: CAD25017.1.
AL590448 Genomic DNA. Translation: CAD26313.1.
RefSeqiNP_597137.1. NM_001041746.1.
XP_965852.1. XM_960759.1.
XP_965982.1. XM_960889.1.

Genome annotation databases

GeneIDi859559.
860188.
860189.
KEGGiecu:ECU01_0170.
ecu:ECU01_1450.
ecu:ECU08_0080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005644 Genomic DNA. Translation: CAA06647.1.
AL391737 Genomic DNA. Translation: CAD24887.1.
AL391737 Genomic DNA. Translation: CAD25017.1.
AL590448 Genomic DNA. Translation: CAD26313.1.
RefSeqiNP_597137.1. NM_001041746.1.
XP_965852.1. XM_960759.1.
XP_965982.1. XM_960889.1.

3D structure databases

ProteinModelPortaliO62583.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6035.ECU08_0080.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi859559.
860188.
860189.
KEGGiecu:ECU01_0170.
ecu:ECU01_1450.
ecu:ECU08_0080.

Phylogenomic databases

eggNOGiCOG0262.
HOGENOMiHOG000040235.
InParanoidiO62583.
KOiK00287.
OMAiPARSTEN.
OrthoDBiEOG7Q8D07.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "First report on the systematic sequencing of the small genome of Encephalitozoon cuniculi (Protozoa, Microspora): gene organization of a 4.3 kbp region on chromosome I."
    Duffieux F., Peyret P., Roe B.A., Vivares C.P.
    Microb. Comp. Genomics 3:1-11(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence and analysis of chromosome I of the amitochondriate intracellular parasite Encephalitozoon cuniculi (Microspora)."
    Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M., Weissenbach J., Saurin W., Vivares C.P.
    Genome Res. 11:198-207(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-M1.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-M1.

Entry informationi

Entry nameiDYR_ENCCU
AccessioniPrimary (citable) accession number: O62583
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: January 7, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.