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O62583

- DYR_ENCCU

UniProt

O62583 - DYR_ENCCU

Protein

Dihydrofolate reductase

Gene

DHFR-1

more
Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.By similarity

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei7 – 71NADP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei78 – 781SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi13 – 197NADPBy similarity
    Nucleotide bindingi62 – 643NADPBy similarity
    Nucleotide bindingi84 – 863NADPBy similarity
    Nucleotide bindingi118 – 1258NADPBy similarity

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: UniProtKB-EC
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. glycine biosynthetic process Source: InterPro
    2. nucleotide biosynthetic process Source: InterPro
    3. one-carbon metabolic process Source: UniProtKB-KW
    4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00077; UER00158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydrofolate reductase (EC:1.5.1.3)
    Gene namesi
    Name:DHFR-1
    Ordered Locus Names:ECU01_0170
    AND
    Name:DHFR-2
    Ordered Locus Names:ECU01_1450
    AND
    Name:DHFR-3
    Ordered Locus Names:ECU08_0080
    OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
    Taxonomic identifieri284813 [NCBI]
    Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
    ProteomesiUP000000819: Chromosome I, UP000000819: Chromosome VIII

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 205205Dihydrofolate reductasePRO_0000186373Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi6035.ECU08_0080.

    Structurei

    3D structure databases

    ProteinModelPortaliO62583.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 201201DHFRPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni29 – 346Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the dihydrofolate reductase family.Curated
    Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0262.
    HOGENOMiHOG000040235.
    KOiK00287.
    OMAiPARSTEN.
    OrthoDBiEOG7Q8D07.

    Family and domain databases

    Gene3Di3.40.430.10. 1 hit.
    InterProiIPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR001796. DHFR_dom.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    [Graphical view]
    PRINTSiPR00070. DHFR.
    SUPFAMiSSF53597. SSF53597. 1 hit.
    PROSITEiPS51330. DHFR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O62583-1 [UniParc]FASTAAdd to Basket

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    MLALVVALAS HRGIGNANAL PWPRPLAADM AWFRTLSQSI PLISPDRIAL    50
    APSASNAVVM GRRTWDSIPS RFRPLANRIN VVLSRGPARS TENTFFIQTF 100
    EALDSLPLPP SSMTFVIGGR DVYSLALESG RPHLIFATEV FESPECDVFF 150
    PHIDWASYEK RDITRDVSRL IDRTLASAFY SPETATFTEN GTSFKMFLYT 200
    KPETR 205
    Length:205
    Mass (Da):22,883
    Last modified:August 1, 1998 - v1
    Checksum:i45450EAA534487DD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005644 Genomic DNA. Translation: CAA06647.1.
    AL391737 Genomic DNA. Translation: CAD24887.1.
    AL391737 Genomic DNA. Translation: CAD25017.1.
    AL590448 Genomic DNA. Translation: CAD26313.1.
    RefSeqiNP_597137.1. NM_001041746.1.
    XP_965852.1. XM_960759.1.
    XP_965982.1. XM_960889.1.

    Genome annotation databases

    GeneIDi859559.
    860188.
    860189.
    KEGGiecu:ECU01_0170.
    ecu:ECU01_1450.
    ecu:ECU08_0080.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005644 Genomic DNA. Translation: CAA06647.1 .
    AL391737 Genomic DNA. Translation: CAD24887.1 .
    AL391737 Genomic DNA. Translation: CAD25017.1 .
    AL590448 Genomic DNA. Translation: CAD26313.1 .
    RefSeqi NP_597137.1. NM_001041746.1.
    XP_965852.1. XM_960759.1.
    XP_965982.1. XM_960889.1.

    3D structure databases

    ProteinModelPortali O62583.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6035.ECU08_0080.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 859559.
    860188.
    860189.
    KEGGi ecu:ECU01_0170.
    ecu:ECU01_1450.
    ecu:ECU08_0080.

    Phylogenomic databases

    eggNOGi COG0262.
    HOGENOMi HOG000040235.
    KOi K00287.
    OMAi PARSTEN.
    OrthoDBi EOG7Q8D07.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .

    Family and domain databases

    Gene3Di 3.40.430.10. 1 hit.
    InterProi IPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR001796. DHFR_dom.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00070. DHFR.
    SUPFAMi SSF53597. SSF53597. 1 hit.
    PROSITEi PS51330. DHFR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "First report on the systematic sequencing of the small genome of Encephalitozoon cuniculi (Protozoa, Microspora): gene organization of a 4.3 kbp region on chromosome I."
      Duffieux F., Peyret P., Roe B.A., Vivares C.P.
      Microb. Comp. Genomics 3:1-11(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence and analysis of chromosome I of the amitochondriate intracellular parasite Encephalitozoon cuniculi (Microspora)."
      Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M., Weissenbach J., Saurin W., Vivares C.P.
      Genome Res. 11:198-207(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GB-M1.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GB-M1.

    Entry informationi

    Entry nameiDYR_ENCCU
    AccessioniPrimary (citable) accession number: O62583
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3