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Protein

Dihydrofolate reductase

Gene

DHFR-1

more
Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (DHFR-1)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei7NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei78SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 19NADPBy similarity7
Nucleotide bindingi62 – 64NADPBy similarity3
Nucleotide bindingi84 – 86NADPBy similarity3
Nucleotide bindingi118 – 125NADPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:DHFR-1
Ordered Locus Names:ECU01_0170
AND
Name:DHFR-2
Ordered Locus Names:ECU01_1450
AND
Name:DHFR-3
Ordered Locus Names:ECU08_0080
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMicrosporidiaUnikaryonidaeEncephalitozoon
Proteomesi
  • UP000000819 Componentsi: Chromosome I, Chromosome VIII

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863731 – 205Dihydrofolate reductaseAdd BLAST205

Interactioni

Protein-protein interaction databases

STRINGi284813.XP_965982.1

Structurei

3D structure databases

ProteinModelPortaliO62583
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 201DHFRPROSITE-ProRule annotationAdd BLAST201

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 34Substrate bindingBy similarity6

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

eggNOGiKOG1324 Eukaryota
COG0262 LUCA
HOGENOMiHOG000040235
InParanoidiO62583
KOiK00287
OMAiMADFDCD
OrthoDBiEOG092C4LJX

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

O62583-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLALVVALAS HRGIGNANAL PWPRPLAADM AWFRTLSQSI PLISPDRIAL
60 70 80 90 100
APSASNAVVM GRRTWDSIPS RFRPLANRIN VVLSRGPARS TENTFFIQTF
110 120 130 140 150
EALDSLPLPP SSMTFVIGGR DVYSLALESG RPHLIFATEV FESPECDVFF
160 170 180 190 200
PHIDWASYEK RDITRDVSRL IDRTLASAFY SPETATFTEN GTSFKMFLYT

KPETR
Length:205
Mass (Da):22,883
Last modified:August 1, 1998 - v1
Checksum:i45450EAA534487DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005644 Genomic DNA Translation: CAA06647.1
AL391737 Genomic DNA Translation: CAD24887.1
AL391737 Genomic DNA Translation: CAD25017.1
AL590448 Genomic DNA Translation: CAD26313.1
RefSeqiNP_597137.1, NM_001041746.1
XP_965852.1, XM_960759.1
XP_965982.1, XM_960889.1

Genome annotation databases

EnsemblFungiiCAD24887; CAD24887; CAD24887
CAD25017; CAD25017; CAD25017
CAD26313; CAD26313; CAD26313
GeneIDi859559
860188
860189
KEGGiecu:ECU01_0170
ecu:ECU01_1450
ecu:ECU08_0080

Similar proteinsi

Entry informationi

Entry nameiDYR_ENCCU
AccessioniPrimary (citable) accession number: O62583
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: May 23, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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