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Protein

Spindle assembly abnormal protein 6

Gene

sas-6

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Central scaffolding component of the centrioles ensuring their 9-fold symmetry. Required for centrosome biogenesis and duplication.2 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein domain specific binding Source: WormBase

GO - Biological processi

  • centriole replication Source: WormBase
  • multicellular organism development Source: UniProtKB-KW
  • protein localization Source: WormBase
  • regulation of cell cycle Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Spindle assembly abnormal protein 6
Gene namesi
Name:sas-6
ORF Names:Y45F10D.9
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiY45F10D.9; CE16647; WBGene00012888; sas-6.

Subcellular locationi

GO - Cellular componenti

  • centriole Source: WormBase
  • cytoplasm Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi154I → D: Homodimerizes but do not form higher ordre structures. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001899781 – 492Spindle assembly abnormal protein 6Add BLAST492

Proteomic databases

EPDiO62479.
PaxDbiO62479.
PeptideAtlasiO62479.

PTM databases

iPTMnetiO62479.

Expressioni

Developmental stagei

Recruited to centrioles at the onset of the centrosome duplication cycle, then associates with the emerging daughter centriole.2 Publications

Gene expression databases

BgeeiWBGene00012888.

Interactioni

Subunit structurei

Nine homodimers form a cartwheel structure with an internal diameter of 23 nM and radial spokes connecting to the microtubule triplets. Interacts with sas-5.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself9EBI-327247,EBI-327247
sas-5Q2001025EBI-327247,EBI-327608

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein domain specific binding Source: WormBase

Protein-protein interaction databases

BioGridi43429. 3 interactors.
DIPiDIP-24495N.
IntActiO62479. 5 interactors.
MINTiMINT-1119015.
STRINGi6239.Y45F10D.9.

Structurei

Secondary structure

1492
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 19Combined sources15
Beta strandi27 – 42Combined sources16
Turni44 – 46Combined sources3
Beta strandi49 – 56Combined sources8
Beta strandi64 – 69Combined sources6
Helixi71 – 81Combined sources11
Turni87 – 89Combined sources3
Helixi90 – 100Combined sources11
Beta strandi132 – 137Combined sources6
Beta strandi141 – 149Combined sources9
Beta strandi153 – 155Combined sources3
Beta strandi158 – 166Combined sources9
Helixi169 – 189Combined sources21
Helixi193 – 196Combined sources4
Helixi199 – 207Combined sources9
Beta strandi252 – 255Combined sources4
Helixi256 – 342Combined sources87
Helixi344 – 348Combined sources5
Helixi350 – 402Combined sources53
Turni403 – 406Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PYIX-ray2.10A/B1-168[»]
4G79X-ray1.80A1-168[»]
4GEUX-ray2.65A/B/C/D1-168[»]
4GEXX-ray2.80A/B/C/D1-168[»]
4GFAX-ray3.55A/B/C/D1-215[»]
4GFCX-ray2.85A/B1-215[»]
4GKWX-ray3.30A/B248-410[»]
ProteinModelPortaliO62479.
SMRiO62479.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO62479.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 98PISAAdd BLAST53

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili192 – 407Sequence analysisAdd BLAST216

Domaini

The coiled coil domain is necessary and sufficient for interaction with sas-5.1 Publication
The 35 nM long coiled-coil domain mediates homodimerization while the globular N-terminus links the dimers at an angle of 40 degrees to form the inner ring.1 Publication

Sequence similaritiesi

Contains 1 PISA domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410K8KZ. Eukaryota.
ENOG4110K83. LUCA.
InParanoidiO62479.
OMAiRGDHLIS.
OrthoDBiEOG091G0FH9.

Family and domain databases

InterProiIPR032396. SAS-6_N.
[Graphical view]
PfamiPF16531. SAS-6_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O62479-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKIALFDQ TLIASLLQPL SLNQPDFKAY KTKVKLKISE QRNETSGEKE
60 70 80 90 100
LKFEISRSDD FEFLFSETLN NEKYQILARD HDLTVDFDAF PKVIIQHLLC
110 120 130 140 150
KNIVKNLEED GEVDARKKAG YHSIADPGKP TEINIILDAE KNFCSFELFS
160 170 180 190 200
KTPISKGKIF SIKLHAVRGD HLISHLLKIC SSQAVKLSTF YKSADELASL
210 220 230 240 250
RQKCGDLEKQ VEKLSGVKEE FEEMSEKFKE LEDEVELVKE ERENIRLLVE
260 270 280 290 300
DKEDEVADLK QDTESLQKQL EENQEELEIV GNMLREEQGK VDQLQKRNVA
310 320 330 340 350
HQKEIGKLRA ELGTAQRNLE KADQLLKRNS QQQNQQSLDM RKLGELEADL
360 370 380 390 400
KEKDSMVESL TETIGILRKE LENEKLKAAE NMDSFEKLSM ENENLKEKIA
410 420 430 440 450
HYRAQRFSPA PSGLPGLQTG LTNRLTPSFK PVLGPHTPYG ANLNSRTPFR
460 470 480 490
DNTTLNFQNS TIATPHAFRF NSQLIADETT GSSVTNTPPA QR
Length:492
Mass (Da):56,038
Last modified:August 1, 1998 - v1
Checksum:i118ED9B5EF6357A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL021492 Genomic DNA. Translation: CAA16384.1.
PIRiT26936.
RefSeqiNP_502660.1. NM_070259.5.
UniGeneiCel.9095.

Genome annotation databases

EnsemblMetazoaiY45F10D.9; Y45F10D.9; WBGene00012888.
GeneIDi178345.
KEGGicel:CELE_Y45F10D.9.
UCSCiY45F10D.9. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL021492 Genomic DNA. Translation: CAA16384.1.
PIRiT26936.
RefSeqiNP_502660.1. NM_070259.5.
UniGeneiCel.9095.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PYIX-ray2.10A/B1-168[»]
4G79X-ray1.80A1-168[»]
4GEUX-ray2.65A/B/C/D1-168[»]
4GEXX-ray2.80A/B/C/D1-168[»]
4GFAX-ray3.55A/B/C/D1-215[»]
4GFCX-ray2.85A/B1-215[»]
4GKWX-ray3.30A/B248-410[»]
ProteinModelPortaliO62479.
SMRiO62479.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi43429. 3 interactors.
DIPiDIP-24495N.
IntActiO62479. 5 interactors.
MINTiMINT-1119015.
STRINGi6239.Y45F10D.9.

PTM databases

iPTMnetiO62479.

Proteomic databases

EPDiO62479.
PaxDbiO62479.
PeptideAtlasiO62479.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY45F10D.9; Y45F10D.9; WBGene00012888.
GeneIDi178345.
KEGGicel:CELE_Y45F10D.9.
UCSCiY45F10D.9. c. elegans.

Organism-specific databases

CTDi43555.
WormBaseiY45F10D.9; CE16647; WBGene00012888; sas-6.

Phylogenomic databases

eggNOGiENOG410K8KZ. Eukaryota.
ENOG4110K83. LUCA.
InParanoidiO62479.
OMAiRGDHLIS.
OrthoDBiEOG091G0FH9.

Miscellaneous databases

EvolutionaryTraceiO62479.
PROiO62479.

Gene expression databases

BgeeiWBGene00012888.

Family and domain databases

InterProiIPR032396. SAS-6_N.
[Graphical view]
PfamiPF16531. SAS-6_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAS6_CAEEL
AccessioniPrimary (citable) accession number: O62479
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.