O62431 (SYQ_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	ers-1
Synonyms:	qrs-5
ORF Names:	Y41E3.4

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	786 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	determination of adult lifespan Inferred from mutant phenotype. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro growth Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 786	786	Probable glutamine--tRNA ligase		PRO_0000195861
Regions
	Motif	276 – 286	11	"HIGH" region
	Motif	499 – 503	5	"KMSKS" region
Sites
	Binding site	502	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O62431 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: D00F343D845F4B47	FASTA	786	88,262
	10 20 30 40 50 60 MATKEELLSL GLSDSKVAET LKNVKLTETI GSIVKLASES GEISKQKGTL LYQLATKLKP 70 80 90 100 110 120 QVAAHTPLVV KYIMNDGIKT EPQLSAAIEY LLSHTVKGIQ VPDFEKSCGV GVVVTIDDIE 130 140 150 160 170 180 AAVTKVIGQH REKIVAERYS FPAGKLLGEL RALLPWADGA ITKKEVDLRF LELLGPKTAE 190 200 210 220 230 240 DLAPKKKEKK PEGPKPSKDA AAAATAPGTK NQKEASPEEF ADGAETMDEL LRTRAHFHKV 250 260 270 280 290 300 GENFKQDGYV TTPKTAELLK AHVAAVGGKV VTRFPPEPNG VLHIGHAKAI NINFGYAKAM 310 320 330 340 350 360 GGVCNLRFDD TNPEKEEEKF FSAIEDIVHW LGYDPARVTH SSDNFQQLYL WAVKLIQKGL 370 380 390 400 410 420 AFVCHQKVEE MRGFEVQLSP WRERPIEENI QLFEDMKNGK FDEGEATLRL KLTLEEGKVD 430 440 450 460 470 480 PVAYRIKYVP HHRTGNQWCI YPTYDYTHCL CDSIENITHS LCTKEFQSRR SSYYWLCNAL 490 500 510 520 530 540 DIYCPVQWEY GRLNVNYTVV SKRKILKLIT TKTVNDWDDP RLFTLTALRR RGIPSEAINR 550 560 570 580 590 600 FVAKLGLTMS QMVIDPHVLD ATVRDYLNIH APRTMAVLEG LKLTIENFSE LNLPSSVDVP 610 620 630 640 650 660 DFPSDPTDPR KHSVSVDREI FIEKSDYKPD DSDKSFRRLT PKQAVGLKHI GLVLRFVKEV 670 680 690 700 710 720 KDAEGHVTEV VVKAEKLSEK DKPKAFIHWV AKPVSCEVRL YDRLFKSKNP EDAQLVPGGF 730 740 750 760 770 780 LSDINPDSLT VVYNALIDQS IAKSKVYDRF QFERIGFFCV DRDSTSSTLV FNRTVMLKDG GASGKN

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z95559 Genomic DNA. Translation: CAB08998.1.
PIR	T26811.
RefSeq	NP_502812.1. NM_070411.5.
UniGene	Cel.7782.
3D structure databases
ProteinModelPortal	O62431.
SMR	O62431. Positions 256-780.
ModBase	Search...
Protein-protein interaction databases
STRING	O62431.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	Y41E3.4a.1; Y41E3.4a.1; Y41E3.4. Y41E3.4a.2; Y41E3.4a.2; Y41E3.4.
GeneID	178415.
KEGG	cel:Y41E3.4.
UCSC	Y41E3.4. c. elegans.
Organism-specific databases
CTD	178415.
WormBase	Y41E3.4a; CE18372; WBGene00001336; qars-1.
Phylogenomic databases
eggNOG	meNOG05516.
GeneTree	EMGT00050000004679.
HOGENOM	HBG334108.
InParanoid	O62431.
OMA	RMPTIAG.
PhylomeDB	O62431.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2. IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 2 hits. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01886.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. PF04558. tRNA_synt_1c_R1. 1 hit. PF04557. tRNA_synt_1c_R2. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. GlnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	901048.

Entry information

Entry name

SYQ_CAEEL

Accession

Primary (citable) accession number: O62431

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project