O61955 (IF4E3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eukaryotic translation initiation factor 4E-3
Short name=eIF-4E-3
Short name=eIF4E-3

Alternative name(s):
eIF-4F 25 kDa subunit
mRNA cap-binding protein

Gene names

Name:	ife-3
ORF Names:	B0348.6

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. All 5 eIF4E proteins bind monomethyl cap structures. Only ife-1, ife-2 and ife-5 bind trimethyl cap structures which result from trans-splicing. Translation of trimethyl cap structure mRNAs may be regulated by intracellular redox state; disulfide bonds change the width and depth of the cap-binding cavity determining selectivity to mRNA caps. Ife-3 is essential for viability. Ref.1 Ref.3 Ref.5
Subunit structure	eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E and eIF4G. eIF4E is also known to interact with other partners.
Tissue specificity	Enriched in the germline. Ref.4
Miscellaneous	Inactivation of ife-3 results in 100% embryonic lethality.
Sequence similarities	Belongs to the eukaryotic initiation factor 4E family.

Ontologies

Keywords
Biological process	Protein biosynthesis Translation regulation
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
Molecular function	Initiation factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: IntAct translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
pqn-45	Q20898	3	EBI-330119,EBI-2001916
T14G10.8	Q22497	4	EBI-330119,EBI-2411746
Y18D10A.8	Q9XW13	6	EBI-330119,EBI-330111

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: O61955-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: O61955-2)

The sequence of this isoform differs from the canonical sequence as follows:
     116-118: Missing.

Isoform c (identifier: O61955-3)

The sequence of this isoform differs from the canonical sequence as follows:
     116-116: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Eukaryotic translation initiation factor 4E-3		PRO_0000193645
Natural variations
	Alternative sequence	116 – 118	3	Missing in isoform b.		VSP_008297
	Alternative sequence	116	1	Missing in isoform c.		VSP_008298

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified December 14, 2011. Version 2. Checksum: EF78008417099EE7	FASTA	251	28,179
	10 20 30 40 50 60 MSTSVAENKA LSASGDVNAS DASVPPELLT RHPLQNRWAL WYLKADRNKE WEDCLKMVSL 70 80 90 100 110 120 FDTVEDFWSL YNHIQSAGGL NWGSDYYLFK EGIKPMWEDV NNVQGGRWLV VVDKQKLQRR 130 140 150 160 170 180 TQLLDHYWLE LLMAIVGEQF DEYGDYICGA VVNVRQKGDK VSLWTRDATR DDVNLRIGQV 190 200 210 220 230 240 LKQKLSIPDT EILRYEVHKD SSARTSSTVK PRICLPAKDP APVKEKGPAA TTSPSNPGTE 250 ATGTSPATPT P
	Isoform b [UniParc]. Checksum: C464C9DEBFA7B5C3	FASTA	248	27,809
	10 20 30 40 50 60 MSTSVAENKA LSASGDVNAS DASVPPELLT RHPLQNRWAL WYLKADRNKE WEDCLKMVSL 70 80 90 100 110 120 FDTVEDFWSL YNHIQSAGGL NWGSDYYLFK EGIKPMWEDV NNVQGGRWLV VVDKQRRTQL 130 140 150 160 170 180 LDHYWLELLM AIVGEQFDEY GDYICGAVVN VRQKGDKVSL WTRDATRDDV NLRIGQVLKQ 190 200 210 220 230 240 KLSIPDTEIL RYEVHKDSSA RTSSTVKPRI CLPAKDPAPV KEKGPAATTS PSNPGTEATG TSPATPTP
	Isoform c [UniParc]. Checksum: 8ED6806DB7066D67	FASTA	250	28,051
	10 20 30 40 50 60 MSTSVAENKA LSASGDVNAS DASVPPELLT RHPLQNRWAL WYLKADRNKE WEDCLKMVSL 70 80 90 100 110 120 FDTVEDFWSL YNHIQSAGGL NWGSDYYLFK EGIKPMWEDV NNVQGGRWLV VVDKQLQRRT 130 140 150 160 170 180 QLLDHYWLEL LMAIVGEQFD EYGDYICGAV VNVRQKGDKV SLWTRDATRD DVNLRIGQVL 190 200 210 220 230 240 KQKLSIPDTE ILRYEVHKDS SARTSSTVKP RICLPAKDPA PVKEKGPAAT TSPSNPGTEA 250 TGTSPATPTP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Multiple isoforms of eukaryotic protein synthesis initiation factor 4E in Caenorhabditis elegans can distinguish between mono- and trimethylated mRNA cap structures." Jankowska-Anyszka M., Lamphear B.J., Aamodt E.J., Harrington T., Darzynkiewicz E., Stolarski R., Rhoads R.E. J. Biol. Chem. 273:10538-10542(1998) [PubMed: 9553113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[3]	"Functional characterization of five eIF4E isoforms in Caenorhabditis elegans." Keiper B.D., Lamphear B.J., Deshpande A.M., Jankowska-Anyszka M., Aamodt E.J., Blumenthal T., Rhoads R.E. J. Biol. Chem. 275:10590-10596(2000) [PubMed: 10744754] [Abstract] Cited for: FUNCTION. Strain: Bristol N2.
[4]	"An isoform of eIF4E is a component of germ granules and is required for spermatogenesis in C. elegans." Amiri A., Keiper B.D., Kawasaki I., Fan Y., Kohara Y., Rhoads R.E., Strome S. Development 128:3899-3912(2001) [PubMed: 11641215] [Abstract] Cited for: TISSUE SPECIFICITY. Strain: Bristol N2.
[5]	"Interaction of three Caenorhabditis elegans isoforms of translation initiation factor eIF4E with mono- and trimethylated mRNA 5' cap analogues." Stachelska A., Wieczorek Z., Ruszczynska K., Stolarski R., Pietrzak M., Lamphear B.J., Rhoads R.E., Darzynkiewicz E., Jankowska-Anyszka M. Acta Biochim. Pol. 49:671-682(2002) [PubMed: 12422237] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080176 Genomic DNA. Translation: CCD61786.1. FO080176 Genomic DNA. Translation: CCD61787.1. FO080176 Genomic DNA. Translation: CCD61788.1.
PIR	T33281.
RefSeq	NP_503123.1. NM_070722.2. NP_503124.1. NM_070723.5. NP_741502.1. NM_171920.2.
UniGene	Cel.32382.
3D structure databases
ProteinModelPortal	O61955.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24919N.
IntAct	O61955. 6 interactions.
MINT	MINT-1101932.
STRING	O61955.
Proteomic databases
PRIDE	O61955.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	B0348.6a.1; B0348.6a.1; B0348.6. B0348.6a.2; B0348.6a.2; B0348.6.
GeneID	178536.
KEGG	cel:B0348.6.
UCSC	B0348.6a.2. c. elegans.
Organism-specific databases
CTD	178536.
WormBase	B0348.6a; CE17331; WBGene00002061; ife-3. B0348.6b; CE27570; WBGene00002061; ife-3. B0348.6c; CE30590; WBGene00002061; ife-3.
Phylogenomic databases
eggNOG	meNOG07654.
GeneTree	EMGT00050000004395.
InParanoid	O61955.
PhylomeDB	O61955.
Gene expression databases
ArrayExpress	O61955.
Family and domain databases
InterPro	IPR023398. TIF_eIF4e-like_dom. IPR001040. TIF_eIF_4E. IPR019770. TIF_eIF_4E_CS. [Graphical view]
Gene3D	G3DSA:3.30.760.10. TIF_eIF_4E. 1 hit.
KO	K03259.
PANTHER	PTHR11960. TIF_eIF_4E. 1 hit.
Pfam	PF01652. IF4E. 1 hit. [Graphical view]
SUPFAM	SSF55418. TIF_eIF_4E. 1 hit.
PROSITE	PS00813. IF4E. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	901526.

Entry information

Entry name

IF4E3_CAEEL

Accession

Primary (citable) accession number: O61955
Secondary accession number(s): Q8MNX5, Q95X31

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 14, 2011
Last modified:	January 25, 2012
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project