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Reviewed, UniProtKB/Swiss-Prot O61955 (IF4E3_CAEEL)

Last modified January 19, 2010. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Eukaryotic translation initiation factor 4E-3
      Short name=eIF-4E-3
      Short name=eIF4E-3
Alternative name(s):
    mRNA cap-binding protein
    eIF-4F 25 kDa subunit
Gene names
Name: ife-3
ORF Names: B0348.6
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. All 5 eIF4E proteins bind monomethyl cap structures. Only ife-1, ife-2 and ife-5 bind trimethyl cap structures which result from trans-splicing. Translation of trimethyl cap structure mRNAs may be regulated by intracellular redox state; disulfide bonds change the width and depth of the cap-binding cavity determining selectivity to mRNA caps. Ife-3 is essential for viability. Ref.1 Ref.3 Ref.5

Subunit structure

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E and eIF4G. eIF4E is also known to interact with other partners.

Tissue specificity

Enriched in the germline. Ref.4

Miscellaneous

Inactivation of ife-3 results in 100% embryonic lethality.

Sequence similarities

Belongs to the eukaryotic initiation factor 4E family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Q9XW132EBI-330119,EBI-330111

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: O61955-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: O61955-2)

The sequence of this isoform differs from the canonical sequence as follows:
     115-117: Missing.
Isoform c (identifier: O61955-3)

The sequence of this isoform differs from the canonical sequence as follows:
     116-116: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 248248Eukaryotic translation initiation factor 4E-3
PRO_0000193645

Natural variations

Alternative sequence115 – 1173Missing in isoform b.
VSP_008297
Alternative sequence1161Missing in isoform c.
VSP_008298

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: C464C9DEBFA7B5C3

FASTA24827,809
        10         20         30         40         50         60 
MSTSVAENKA LSASGDVNAS DASVPPELLT RHPLQNRWAL WYLKADRNKE WEDCLKMVSL 

        70         80         90        100        110        120 
FDTVEDFWSL YNHIQSAGGL NWGSDYYLFK EGIKPMWEDV NNVQGGRWLV VVDKQRRTQL 

       130        140        150        160        170        180 
LDHYWLELLM AIVGEQFDEY GDYICGAVVN VRQKGDKVSL WTRDATRDDV NLRIGQVLKQ 

       190        200        210        220        230        240 
KLSIPDTEIL RYEVHKDSSA RTSSTVKPRI CLPAKDPAPV KEKGPAATTS PSNPGTEATG 


TSPATPTP 

« Hide

Isoform b.

Checksum: 2ED59EC2558D0572
Show »

FASTA24527,369
Isoform c.

Checksum: CDC4D91037E29059
Show »

FASTA24727,653

References

« Hide 'large scale' references
[1]"Multiple isoforms of eukaryotic protein synthesis initiation factor 4E in Caenorhabditis elegans can distinguish between mono- and trimethylated mRNA cap structures."
Jankowska-Anyszka M., Lamphear B.J., Aamodt E.J., Harrington T., Darzynkiewicz E., Stolarski R., Rhoads R.E.
J. Biol. Chem. 273:10538-10542(1998) [PubMed: 9553113] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION.
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[3]"Functional characterization of five eIF4E isoforms in Caenorhabditis elegans."
Keiper B.D., Lamphear B.J., Deshpande A.M., Jankowska-Anyszka M., Aamodt E.J., Blumenthal T., Rhoads R.E.
J. Biol. Chem. 275:10590-10596(2000) [PubMed: 10744754] [Abstract]
Cited for: FUNCTION.
Strain: Bristol N2.
[4]"An isoform of eIF4E is a component of germ granules and is required for spermatogenesis in C. elegans."
Amiri A., Keiper B.D., Kawasaki I., Fan Y., Kohara Y., Rhoads R.E., Strome S.
Development 128:3899-3912(2001) [PubMed: 11641215] [Abstract]
Cited for: TISSUE SPECIFICITY.
Strain: Bristol N2.
[5]"Interaction of three Caenorhabditis elegans isoforms of translation initiation factor eIF4E with mono- and trimethylated mRNA 5' cap analogues."
Stachelska A., Wieczorek Z., Ruszczynska K., Stolarski R., Pietrzak M., Lamphear B.J., Rhoads R.E., Darzynkiewicz E., Jankowska-Anyszka M.
Acta Biochim. Pol. 49:671-682(2002) [PubMed: 12422237] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF068714 Genomic DNA. Translation: AAC17807.1.
AF068714 Genomic DNA. Translation: AAK68676.1.
AF068714 Genomic DNA. Translation: AAM22022.1.
PIRT33281.
RefSeqNP_503123.1.
NP_503124.1.
NP_741502.1.
UniGeneCel.32382

3D structure databases

SMRO61955. Positions 3-212.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-24919N.
IntActO61955. 6 interactions.
STRINGO61955.

Proteomic databases

PRIDEO61955.

Genome annotation databases

EnsemblB0348.6a.1; B0348.6a.1; B0348.6; Caenorhabditis elegans. [Genome view]
B0348.6a.2; B0348.6a.2; B0348.6; Caenorhabditis elegans. [Genome view]
GeneID178536.
KEGGcel:B0348.6.
UCSCB0348.6a.2. c. elegans.

Organism-specific databases

CTD178536.
WormBaseWBGene00002061. ife-3.
WormPepB0348.6a. CE17331. [WorfDB]
B0348.6b. CE27570. [WorfDB]
B0348.6c. CE30590. [WorfDB]

Phylogenomic databases

eggNOGmeNOG07654.
InParanoidO61955.
PhylomeDBO61955.

Gene expression databases

ArrayExpressO61955.

Family and domain databases

InterProIPR001040. TIF_eIF_4E.
IPR019770. TIF_eIF_4E_CS.
[Graphical view]
Gene3DG3DSA:3.30.760.10. TIF_eIF_4E. 1 hit.
PANTHERPTHR11960. TIF_eIF_4E. 1 hit.
PfamPF01652. IF4E. 1 hit.
[Graphical view]
PROSITEPS00813. IF4E. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio901526.

Entry information

Entry nameIF4E3_CAEEL
AccessionPrimary (citable) accession number: O61955
Secondary accession number(s): Q8MNX5, Q95X31
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: January 19, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents