O61955 (IF4E3_CAEEL) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 83.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Eukaryotic translation initiation factor 4E-3 Short name=eIF-4E-3 Short name=eIF4E-3 Alternative name(s): eIF-4F 25 kDa subunit mRNA cap-binding protein | ||||
| Gene names |
| ||||
| Organism | Caenorhabditis elegans | ||||
| Taxonomic identifier | 6239 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Protein attributes
| Sequence length | 251 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. All 5 eIF4E proteins bind monomethyl cap structures. Only ife-1, ife-2 and ife-5 bind trimethyl cap structures which result from trans-splicing. Translation of trimethyl cap structure mRNAs may be regulated by intracellular redox state; disulfide bonds change the width and depth of the cap-binding cavity determining selectivity to mRNA caps. Ife-3 is essential for viability. Ref.1 Ref.3 Ref.5 |
| Subunit structure | eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E and eIF4G. eIF4E is also known to interact with other partners. |
| Tissue specificity | Enriched in the germline. Ref.4 |
| Miscellaneous | Inactivation of ife-3 results in 100% embryonic lethality. |
| Sequence similarities | Belongs to the eukaryotic initiation factor 4E family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein biosynthesis Translation regulation |
| Coding sequence diversity | Alternative splicing |
| Ligand | RNA-binding |
| Molecular function | Initiation factor |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase regulation of translationInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: InterPro |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct translation initiation factor activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| pqn-45 | Q20898 | 3 | EBI-330119,EBI-2001916 | |
| T14G10.8 | Q22497 | 4 | EBI-330119,EBI-2411746 | |
| Y18D10A.8 | Q9XW13 | 6 | EBI-330119,EBI-330111 |
Alternative products
| This entry describes 3 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform a (identifier: O61955-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform b (identifier: O61955-2) The sequence of this isoform differs from the canonical sequence as follows: 116-118: Missing. | ||||||
| Isoform c (identifier: O61955-3) The sequence of this isoform differs from the canonical sequence as follows: 116-116: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 251 | 251 | Eukaryotic translation initiation factor 4E-3 | PRO_0000193645 | |||||
Natural variations | |||||||||
| Alternative sequence | 116 – 118 | 3 | Missing in isoform b. | VSP_008297 | |||||
| Alternative sequence | 116 | 1 | Missing in isoform c. | VSP_008298 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Multiple isoforms of eukaryotic protein synthesis initiation factor 4E in Caenorhabditis elegans can distinguish between mono- and trimethylated mRNA cap structures." Jankowska-Anyszka M., Lamphear B.J., Aamodt E.J., Harrington T., Darzynkiewicz E., Stolarski R., Rhoads R.E. J. Biol. Chem. 273:10538-10542(1998) [PubMed: 9553113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION. Strain: Bristol N2. |
| [2] | "Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2. |
| [3] | "Functional characterization of five eIF4E isoforms in Caenorhabditis elegans." Keiper B.D., Lamphear B.J., Deshpande A.M., Jankowska-Anyszka M., Aamodt E.J., Blumenthal T., Rhoads R.E. J. Biol. Chem. 275:10590-10596(2000) [PubMed: 10744754] [Abstract] Cited for: FUNCTION. Strain: Bristol N2. |
| [4] | "An isoform of eIF4E is a component of germ granules and is required for spermatogenesis in C. elegans." Amiri A., Keiper B.D., Kawasaki I., Fan Y., Kohara Y., Rhoads R.E., Strome S. Development 128:3899-3912(2001) [PubMed: 11641215] [Abstract] Cited for: TISSUE SPECIFICITY. Strain: Bristol N2. |
| [5] | "Interaction of three Caenorhabditis elegans isoforms of translation initiation factor eIF4E with mono- and trimethylated mRNA 5' cap analogues." Stachelska A., Wieczorek Z., Ruszczynska K., Stolarski R., Pietrzak M., Lamphear B.J., Rhoads R.E., Darzynkiewicz E., Jankowska-Anyszka M. Acta Biochim. Pol. 49:671-682(2002) [PubMed: 12422237] [Abstract] Cited for: FUNCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | FO080176 Genomic DNA. Translation: CCD61786.1. FO080176 Genomic DNA. Translation: CCD61787.1. FO080176 Genomic DNA. Translation: CCD61788.1. |
| PIR | T33281. |
| RefSeq | NP_503123.1. NM_070722.2. NP_503124.1. NM_070723.5. NP_741502.1. NM_171920.2. |
| UniGene | Cel.32382. |
3D structure databases | |
| ProteinModelPortal | O61955. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-24919N. |
| IntAct | O61955. 6 interactions. |
| MINT | MINT-1101932. |
| STRING | O61955. |
Proteomic databases | |
| PRIDE | O61955. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblMetazoa | B0348.6a.1; B0348.6a.1; B0348.6. B0348.6a.2; B0348.6a.2; B0348.6. |
| GeneID | 178536. |
| KEGG | cel:B0348.6. |
| UCSC | B0348.6a.2. c. elegans. |
Organism-specific databases | |
| CTD | 178536. |
| WormBase | B0348.6a; CE17331; WBGene00002061; ife-3. B0348.6b; CE27570; WBGene00002061; ife-3. B0348.6c; CE30590; WBGene00002061; ife-3. |
Phylogenomic databases | |
| eggNOG | meNOG07654. |
| GeneTree | EMGT00050000004395. |
| InParanoid | O61955. |
| PhylomeDB | O61955. |
Gene expression databases | |
| ArrayExpress | O61955. |
Family and domain databases | |
| InterPro | IPR023398. TIF_eIF4e-like_dom. IPR001040. TIF_eIF_4E. IPR019770. TIF_eIF_4E_CS. [Graphical view] |
| Gene3D | G3DSA:3.30.760.10. TIF_eIF_4E. 1 hit. |
| KO | K03259. |
| PANTHER | PTHR11960. TIF_eIF_4E. 1 hit. |
| Pfam | PF01652. IF4E. 1 hit. [Graphical view] |
| SUPFAM | SSF55418. TIF_eIF_4E. 1 hit. |
| PROSITE | PS00813. IF4E. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 901526. |
Entry information
| Entry name | IF4E3_CAEEL | ||||||||
| Accession | Primary (citable) accession number: O61955 Secondary accession number(s): Q8MNX5, Q95X31 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Caenorhabditis annotation project | ||||||||
Relevant documents
| Caenorhabditis elegans Caenorhabditis elegans: entries, gene names and cross-references to WormPep |
| SIMILARITY comments Index of protein domains and families |

Clusters with