Eukaryotic translation initiation factor 4E-3

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Reviewed, UniProtKB/Swiss-Prot O61955 (IF4E3_CAEEL)

Last modified January 19, 2010. Version 68. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Eukaryotic translation initiation factor 4E-3
      Short name=eIF-4E-3
      Short name=eIF4E-3
Alternative name(s):
    mRNA cap-binding protein
    eIF-4F 25 kDa subunit

Gene names

Name:	ife-3
ORF Names:	B0348.6

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. All 5 eIF4E proteins bind monomethyl cap structures. Only ife-1, ife-2 and ife-5 bind trimethyl cap structures which result from trans-splicing. Translation of trimethyl cap structure mRNAs may be regulated by intracellular redox state; disulfide bonds change the width and depth of the cap-binding cavity determining selectivity to mRNA caps. Ife-3 is essential for viability. Ref.1 Ref.3 Ref.5
Subunit structure	eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E and eIF4G. eIF4E is also known to interact with other partners.
Tissue specificity	Enriched in the germline. Ref.4
Miscellaneous	Inactivation of ife-3 results in 100% embryonic lethality.
Sequence similarities	Belongs to the eukaryotic initiation factor 4E family.

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Ontologies

Keywords
Biological process	Protein biosynthesis Translation regulation
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
Molecular function	Initiation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW translational initiation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9XW13	2	EBI-330119,EBI-330111

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: O61955-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: O61955-2) The sequence of this isoform differs from the canonical sequence as follows: 115-117: Missing.

Isoform c (identifier: O61955-3) The sequence of this isoform differs from the canonical sequence as follows: 116-116: Missing.
Note: No experimental confirmation available.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 248

248

Eukaryotic translation initiation factor 4E-3

PRO_0000193645

Natural variations

Alternative sequence

115 – 117

Missing in isoform b.

VSP_008297

Alternative sequence

116

Missing in isoform c.

VSP_008298

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Sequences

Sequence		Length	Mass (Da)
Isoform a [UniParc]. Last modified August 1, 1998. Version 1. Checksum: C464C9DEBFA7B5C3 Show »	FASTA	248	27,809
10 20 30 40 50 60 MSTSVAENKA LSASGDVNAS DASVPPELLT RHPLQNRWAL WYLKADRNKE WEDCLKMVSL 70 80 90 100 110 120 FDTVEDFWSL YNHIQSAGGL NWGSDYYLFK EGIKPMWEDV NNVQGGRWLV VVDKQRRTQL 130 140 150 160 170 180 LDHYWLELLM AIVGEQFDEY GDYICGAVVN VRQKGDKVSL WTRDATRDDV NLRIGQVLKQ 190 200 210 220 230 240 KLSIPDTEIL RYEVHKDSSA RTSSTVKPRI CLPAKDPAPV KEKGPAATTS PSNPGTEATG TSPATPTP « Hide
Isoform b. Checksum: 2ED59EC2558D0572 Show »	FASTA	245	27,369
10 20 30 40 50 60 MSTSVAENKA LSASGDVNAS DASVPPELLT RHPLQNRWAL WYLKADRNKE WEDCLKMVSL 70 80 90 100 110 120 FDTVEDFWSL YNHIQSAGGL NWGSDYYLFK EGIKPMWEDV NNVQGGRWLV VVDKTQLLDH 130 140 150 160 170 180 YWLELLMAIV GEQFDEYGDY ICGAVVNVRQ KGDKVSLWTR DATRDDVNLR IGQVLKQKLS 190 200 210 220 230 240 IPDTEILRYE VHKDSSARTS STVKPRICLP AKDPAPVKEK GPAATTSPSN PGTEATGTSP ATPTP « Hide
Isoform c. Checksum: CDC4D91037E29059 Show »	FASTA	247	27,653
10 20 30 40 50 60 MSTSVAENKA LSASGDVNAS DASVPPELLT RHPLQNRWAL WYLKADRNKE WEDCLKMVSL 70 80 90 100 110 120 FDTVEDFWSL YNHIQSAGGL NWGSDYYLFK EGIKPMWEDV NNVQGGRWLV VVDKQRTQLL 130 140 150 160 170 180 DHYWLELLMA IVGEQFDEYG DYICGAVVNV RQKGDKVSLW TRDATRDDVN LRIGQVLKQK 190 200 210 220 230 240 LSIPDTEILR YEVHKDSSAR TSSTVKPRIC LPAKDPAPVK EKGPAATTSP SNPGTEATGT SPATPTP « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Multiple isoforms of eukaryotic protein synthesis initiation factor 4E in Caenorhabditis elegans can distinguish between mono- and trimethylated mRNA cap structures." Jankowska-Anyszka M., Lamphear B.J., Aamodt E.J., Harrington T., Darzynkiewicz E., Stolarski R., Rhoads R.E. J. Biol. Chem. 273:10538-10542(1998) [PubMed: 9553113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[3]	"Functional characterization of five eIF4E isoforms in Caenorhabditis elegans." Keiper B.D., Lamphear B.J., Deshpande A.M., Jankowska-Anyszka M., Aamodt E.J., Blumenthal T., Rhoads R.E. J. Biol. Chem. 275:10590-10596(2000) [PubMed: 10744754] [Abstract] Cited for: FUNCTION. Strain: Bristol N2.
[4]	"An isoform of eIF4E is a component of germ granules and is required for spermatogenesis in C. elegans." Amiri A., Keiper B.D., Kawasaki I., Fan Y., Kohara Y., Rhoads R.E., Strome S. Development 128:3899-3912(2001) [PubMed: 11641215] [Abstract] Cited for: TISSUE SPECIFICITY. Strain: Bristol N2.
[5]	"Interaction of three Caenorhabditis elegans isoforms of translation initiation factor eIF4E with mono- and trimethylated mRNA 5' cap analogues." Stachelska A., Wieczorek Z., Ruszczynska K., Stolarski R., Pietrzak M., Lamphear B.J., Rhoads R.E., Darzynkiewicz E., Jankowska-Anyszka M. Acta Biochim. Pol. 49:671-682(2002) [PubMed: 12422237] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AF068714 Genomic DNA. Translation: AAC17807.1. AF068714 Genomic DNA. Translation: AAK68676.1. AF068714 Genomic DNA. Translation: AAM22022.1.
PIR	T33281.
RefSeq	NP_503123.1. NP_503124.1. NP_741502.1.
UniGene	Cel.32382
3D structure databases
SMR	O61955. Positions 3-212.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24919N.
IntAct	O61955. 6 interactions.
STRING	O61955.
Proteomic databases
PRIDE	O61955.
Genome annotation databases
Ensembl	B0348.6a.1; B0348.6a.1; B0348.6; Caenorhabditis elegans. [Genome view] B0348.6a.2; B0348.6a.2; B0348.6; Caenorhabditis elegans. [Genome view]
GeneID	178536.
KEGG	cel:B0348.6.
UCSC	B0348.6a.2. c. elegans.
Organism-specific databases
CTD	178536.
WormBase	WBGene00002061. ife-3.
WormPep	B0348.6a. CE17331. [WorfDB] B0348.6b. CE27570. [WorfDB] B0348.6c. CE30590. [WorfDB]
Phylogenomic databases
eggNOG	meNOG07654.
InParanoid	O61955.
PhylomeDB	O61955.
Gene expression databases
ArrayExpress	O61955.
Family and domain databases
InterPro	IPR001040. TIF_eIF_4E. IPR019770. TIF_eIF_4E_CS. [Graphical view]
Gene3D	G3DSA:3.30.760.10. TIF_eIF_4E. 1 hit.
PANTHER	PTHR11960. TIF_eIF_4E. 1 hit.
Pfam	PF01652. IF4E. 1 hit. [Graphical view]
PROSITE	PS00813. IF4E. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	901526.

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Entry information

Entry name

IF4E3_CAEEL

Accession

Primary (citable) accession number: O61955
Secondary accession number(s): Q8MNX5, Q95X31

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	January 19, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents