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Protein

Inactive protein-tyrosine phosphatase egg-5

Gene

egg-5

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inactive phosphatase which acts redundantly with egg-4 in the oocyte-to-zygote transition (PubMed:19879842, PubMed:19879147). Required for polarized cortical actin cytoskeleton rearrangement in the oocyte before and after fertilization (PubMed:19879147). Together with egg-4, required for the cortical localization of kinase mbk-2 in maturing oocyte until the end of meiosis I (PubMed:19879842). Also required for kinase mbk-2, pseudophosphatase egg-3 and chitin synthase chs-1 localization to cytoplasmic foci after fertilization (PubMed:19879147).2 Publications

Caution

The active site Cys-600 is replaced by a Asp residue suggesting that egg-5 may lack catalytic activity. Despite the lack of catalytic activity, egg-5 may retain the capacity to bind to phosphorylated substrates.Curated

GO - Molecular functioni

  • protein kinase binding Source: WormBase
  • protein tyrosine phosphatase activity Source: InterPro

GO - Biological processi

  • cortical actin cytoskeleton organization Source: UniProtKB
  • eggshell formation Source: UniProtKB
  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of protein kinase activity Source: WormBase
  • polar body extrusion after meiotic divisions Source: UniProtKB
  • positive regulation of protein localization to cell cortex Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Inactive protein-tyrosine phosphatase egg-5Curated
Alternative name(s):
Egg sterile protein 5Imported
Protein-tyrosine phosphatase-like protein egg-51 Publication
Gene namesi
Name:egg-5Imported
ORF Names:R12E2.10Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiR12E2.10 ; CE18142 ; WBGene00020035 ; egg-5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Severe reduction in the number of hatched larvae (PubMed:19879147). Simultaneous RNAi-mediated knockdown of egg-5 results in no viable progeny and causes endomitosis in the uterus, generation of polyspermic embryos and defects in meiosis completion, polar body formation and eggshell chitin layer formation (PubMed:19879147). In addition, posterior formation of F-actin cap at the cortex oocyte and its polarized dispersal after fertilization are impaired (PubMed:19879147). Prevents mbk-2 localization to the unfertilized oocyte cortex without affecting egg-3 cortical localization (PubMed:19879842, PubMed:19879147). Prevents mbk-2, egg-3 and chs-1 re-localization to cytoplasmic foci at meiosis anaphase I (PubMed:19879147). Simultaneous RNAi-mediated knockdown of egg-5 and mel-26 causes premature phosphorylation of mei-1, a mbk-1 substrate, during oocyte maturation (PubMed:19879842).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004432521 – 753Inactive protein-tyrosine phosphatase egg-5Add BLAST753

Proteomic databases

EPDiO61789
PaxDbiO61789
PeptideAtlasiO61789

Expressioni

Gene expression databases

BgeeiWBGene00020035

Interactioni

Subunit structurei

Part of a complex, consisting of pseudophosphatases egg-3, egg-4, egg-5 and kinase mbk-2; this complex is required for the oocyte-to-zygote transition. Interacts (via tyrosine-protein phosphatase domain) with kinase mbk-2 (via 'Tyr-619' and 'Tyr-621'); mbk-2 tyrosine phosphorylation enhances the interaction.1 Publication

GO - Molecular functioni

  • protein kinase binding Source: WormBase

Protein-protein interaction databases

ComplexPortaliCPX-3381 Egg-3/4/5 MBK-2 complex
IntActiO61789, 1 interactor
STRINGi6239.R12E2.10

Structurei

3D structure databases

ProteinModelPortaliO61789
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini408 – 661Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0789 Eukaryota
COG5599 LUCA
GeneTreeiENSGT00920000148980
HOGENOMiHOG000113912
InParanoidiO61789
OrthoDBiEOG091G02QK

Family and domain databases

Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR029021 Prot-tyrosine_phosphatase-like
IPR000242 PTPase_domain
IPR003595 Tyr_Pase_cat
IPR000387 TYR_PHOSPHATASE_dom
PfamiView protein in Pfam
PF00102 Y_phosphatase, 1 hit
PRINTSiPR00700 PRTYPHPHTASE
SMARTiView protein in SMART
SM00194 PTPc, 1 hit
SM00404 PTPc_motif, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50055 TYR_PHOSPHATASE_PTP, 1 hit

Sequencei

Sequence statusi: Complete.

O61789-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALNSEVMFR EQINAMRSQA GRKRATSLQS FCSGNTDDSS ADSTDNMDMM
60 70 80 90 100
VDYPQQKGVS CMRARFNSES TLSKSFRKKV KKLAQKDRRS KERLNGNSEE
110 120 130 140 150
DAIEVPRGAP STYAAPSKLR KSKALDCLVS EKPKDEGRSE DSGHGADIEM
160 170 180 190 200
AKGHFNDVRM KVFAARTAMQ VEPALVMKTR KALEMKNAVL ENHQSPGAFS
210 220 230 240 250
LHAAYKIAAS AESRVGSITP CNKKVTKEAM ANLIRSSYDD TEITQELLFS
260 270 280 290 300
SKFDSKWKGR YTDIYMRRDE NGKKPKRPVN GQGWVMPLKS ICEKFGINST
310 320 330 340 350
FFTNHRIDLK SARDQVLLMR LLSHDQTSTW ISDIHPEAVK NETLAEYLLR
360 370 380 390 400
ELDASTMQKR VQAFKANVLA DRDRVRVAGQ FYNNIRIGKR MFGAARKAKY
410 420 430 440 450
LSTIIGGMER RFEILENSVN HIPFTHSASD NNQEKCRNPR VHCRDSTRIA
460 470 480 490 500
LQFPRGQYLG DFIHANRISG KPLFNEFIMT QAPMKNTVDD FWRMVWQEEV
510 520 530 540 550
PYIVMLTSRK EPERCEYYWP KSPSDPAVTV PGGLRIENFG VYQAPDPLFR
560 570 580 590 600
VTHLRLIGPD REERHVEHWQ GDVNNSSNMY SPLNILRLLR NASKPVVIHD
610 620 630 640 650
HLGVSRAACL VAAEIAICSL LRGPTYKYPV QRAVQFLRQR RPFSIETPMQ
660 670 680 690 700
YIFVHRLVAF FFRDVIGSAK ELDVDYERWL QERSERMFLD DLAAPIPGYR
710 720 730 740 750
LLSPRADPDI VRMVGRPERP NYRREAPDCV GEMPNKVAAV DGILSPAKSV

FEF
Length:753
Mass (Da):85,957
Last modified:August 1, 1998 - v1
Checksum:iDB71183AB91FD14F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA Translation: CCD65177.1
PIRiT33093
RefSeqiNP_491316.1, NM_058915.6
UniGeneiCel.21334

Genome annotation databases

EnsemblMetazoaiR12E2.10; R12E2.10; WBGene00020035
GeneIDi172007
KEGGicel:CELE_R12E2.10
UCSCiR12E2.10 c. elegans

Similar proteinsi

Entry informationi

Entry nameiEGG5_CAEEL
AccessioniPrimary (citable) accession number: O61789
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2018
Last sequence update: August 1, 1998
Last modified: June 20, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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