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Protein

26S proteasome non-ATPase regulatory subunit 4

Gene

rpn-10

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Binds and presumably selects ubiquitin-conjugates for destruction (By similarity). Required for protein degradation and ubiquitin-proteasome system (UBS) function and regulates proteasomal subunit expression (PubMed:20453865, PubMed:22105480, PubMed:26828939). Involvement in UBS might be cell type specific (PubMed:20453865). Regulator of the autophagy-lysosome pathway that may confer resistance to autophagy by regulating the expression of autophagy-related proteins such as lgg-1, and by regulating lysosome formation, possibly by modulating elt-2 activity (PubMed:26828939). Required for fertility, sperm production, and sex determination through regulation of tra-2 protein (PubMed:17050737). Plays a role in the elimination of paternal mitochondria in fertilized eggs (PubMed:22105480).By similarity4 Publications

GO - Molecular functioni

GO - Biological processi

  • proteasome assembly Source: GO_Central
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  • spermatogenesis Source: WormBase
  • ubiquitin-dependent protein catabolic process Source: WormBase
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-CEL-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-CEL-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-CEL-382556. ABC-family proteins mediated transport.
R-CEL-4641258. Degradation of DVL.
R-CEL-5632684. Hedgehog 'on' state.
R-CEL-5689603. UCH proteinases.
R-CEL-5689880. Ub-specific processing proteases.
R-CEL-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-CEL-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-CEL-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-CEL-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 4By similarity
Alternative name(s):
26S proteasome regulatory subunit rpn-10Curated
Gene namesi
Name:rpn-10Imported
ORF Names:B0205.3Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiB0205.3; CE26355; WBGene00004466; rpn-10.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Disruption phenotypei

Temperature-dependent increase in lifespan with a modest increase at 20 degrees Celsius and a 30% increase in mean lifespan at 25 degrees Celsius compared to wild-type animals (PubMed:26828939). Reduced fertility with no sperm produced in the spermatheca, accumulation of a sex-determination protein tra-2 in the intestine, no embryos in the uterus, and an increase in the development time to adulthood as evidenced by an expansion of the proximal oocytes at a young adult stage (PubMed:17050737, PubMed:26828939). Reduced ubiquitin-proteasome system function (UBS), particularly in dorsorectal neurons (PubMed:20453865, PubMed:26828939). Enhanced protein stability and increased resistance to protostasis in response to thermal stress and oxidative stress induced by tert-butyl hydroperoxide (PubMed:26828939). Irregular autophagy-lysosome pathway function with increased autophagy coupled with fewer intestinal lysosomes and reduced lysosomal function (PubMed:26828939). RNAi-mediated knockdown of skn-1 or elt-2 in the null mutant results in animals which are smaller and that developmentally arrest before adulthood (PubMed:26828939). RNAi-mediated knockdown results in reduced egg laying, abnormal gonad morphology with expansion of the proximal gonad greater than that of wild-type animals, and defective degradation of polyubiquitinated proteins and leads to an accumulation of these proteins (PubMed:17050737). Also results in delayed removal of paternal mitochondria until 4-fold stage of embryonic development (PubMed:22105480).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004367801 – 34626S proteasome non-ATPase regulatory subunit 4CuratedAdd BLAST346

Proteomic databases

EPDiO61742.
PaxDbiO61742.
PeptideAtlasiO61742.

Expressioni

Tissue specificityi

Broadly expressed with high expression in the pharynx, intestine, hypodermis and spermatheca and weak expression in the excretory cell, body wall muscle, vulva and somatic gonad.1 Publication

Gene expression databases

BgeeiWBGene00004466.

Interactioni

Subunit structurei

The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiO61742. 22 interactors.
MINTiMINT-210565.
STRINGi6239.B0205.3.2.

Structurei

3D structure databases

ProteinModelPortaliO61742.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 190VWFAPROSITE-ProRule annotationAdd BLAST186
Domaini216 – 235UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini273 – 292UIM 2PROSITE-ProRule annotationAdd BLAST20

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi234 – 261Ala-richPROSITE-ProRule annotationAdd BLAST28

Sequence similaritiesi

Belongs to the proteasome subunit S5A family.Curated
Contains 2 UIM (ubiquitin-interacting motif) domains.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2884. Eukaryota.
COG5148. LUCA.
GeneTreeiENSGT00530000064050.
HOGENOMiHOG000165630.
InParanoidiO61742.
KOiK03029.
OMAiRIIVFVC.
OrthoDBiEOG091G0A6L.
PhylomeDBiO61742.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR027040. Proteasome_su_Rpn10.
IPR003903. UIM_dom.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10223. PTHR10223. 1 hit.
PfamiPF02809. UIM. 2 hits.
PF13519. VWA_2. 1 hit.
[Graphical view]
SMARTiSM00726. UIM. 2 hits.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50330. UIM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O61742-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQESTMICV DNSEYMRNGD FQPTRLQSQQ DAVNLVTQCK LRANPENAVG
60 70 80 90 100
ILSMANSVQV LSSLSTEQGR LMMKNHSIEP FGKCNFIAGI KIAHLALKHR
110 120 130 140 150
QNRNHKMRVV LFIGSPLEEI EMNELVKIAK KMKKEKVLCD VIMFGENESD
160 170 180 190 200
GHEKFSTFVD TLNGKEGSGS SLIVVPQGSS LTDALLQSSV CKNEDGQAAF
210 220 230 240 250
GGGGNGMDNA FGMDVENDPD LALALRVSME EERARQAAAA AANGGAADSG
260 270 280 290 300
ADAEVAAAAA AVPLEEMDMG AMTEEQQLEW ALRLSMQENA PAEQPQVQHE
310 320 330 340
QMDVDGAPAV GGDNLDDLMN NPELLQQIVD DLPAANAEKD DDKEKK
Length:346
Mass (Da):37,348
Last modified:October 1, 2001 - v2
Checksum:iADAFB35DE60BDE48
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA. Translation: CCD61298.1.
RefSeqiNP_492809.1. NM_060408.4.
UniGeneiCel.5538.

Genome annotation databases

EnsemblMetazoaiB0205.3.1; B0205.3.1; WBGene00004466.
B0205.3.2; B0205.3.2; WBGene00004466.
GeneIDi172977.
KEGGicel:CELE_B0205.3.
UCSCiB0205.3.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA. Translation: CCD61298.1.
RefSeqiNP_492809.1. NM_060408.4.
UniGeneiCel.5538.

3D structure databases

ProteinModelPortaliO61742.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO61742. 22 interactors.
MINTiMINT-210565.
STRINGi6239.B0205.3.2.

Proteomic databases

EPDiO61742.
PaxDbiO61742.
PeptideAtlasiO61742.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiB0205.3.1; B0205.3.1; WBGene00004466.
B0205.3.2; B0205.3.2; WBGene00004466.
GeneIDi172977.
KEGGicel:CELE_B0205.3.
UCSCiB0205.3.1. c. elegans.

Organism-specific databases

CTDi172977.
WormBaseiB0205.3; CE26355; WBGene00004466; rpn-10.

Phylogenomic databases

eggNOGiKOG2884. Eukaryota.
COG5148. LUCA.
GeneTreeiENSGT00530000064050.
HOGENOMiHOG000165630.
InParanoidiO61742.
KOiK03029.
OMAiRIIVFVC.
OrthoDBiEOG091G0A6L.
PhylomeDBiO61742.

Enzyme and pathway databases

ReactomeiR-CEL-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-CEL-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-CEL-382556. ABC-family proteins mediated transport.
R-CEL-4641258. Degradation of DVL.
R-CEL-5632684. Hedgehog 'on' state.
R-CEL-5689603. UCH proteinases.
R-CEL-5689880. Ub-specific processing proteases.
R-CEL-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-CEL-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-CEL-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-CEL-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiO61742.

Gene expression databases

BgeeiWBGene00004466.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR027040. Proteasome_su_Rpn10.
IPR003903. UIM_dom.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10223. PTHR10223. 1 hit.
PfamiPF02809. UIM. 2 hits.
PF13519. VWA_2. 1 hit.
[Graphical view]
SMARTiSM00726. UIM. 2 hits.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50330. UIM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSMD4_CAEEL
AccessioniPrimary (citable) accession number: O61742
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 6, 2016
Last sequence update: October 1, 2001
Last modified: November 30, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.