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O61734 (CYCL_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein cycle
Alternative name(s):
Brain and muscle ARNT-like 1
Short name=BMAL1
MOP3
Gene names
Name:cyc
ORF Names:CG8727
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length413 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Putative transcription factor involved in the generation of biological rhythms. Activates cycling transcription of Period (PER) and Timeless (TIM) by binding to the E-box (5'-CACGTG-3') present in their promoters.

Subunit structure

Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with Clock in order to activate PER and TIM transcription.

Subcellular location

Nucleus Potential.

Tissue specificity

Expressed in head and ovary.

Disruption phenotype

Cyc mutants don't display PER and TIM cycling, and are completely arrhythmic. Ref.1

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 2 PAS (PER-ARNT-SIM) domains.

Ontologies

Keywords
   Biological processBiological rhythms
Transcription
Transcription regulation
   Cellular componentNucleus
   DomainRepeat
   LigandDNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbehavioral response to cocaine

Non-traceable author statement PubMed 11715043. Source: FlyBase

circadian regulation of gene expression

Inferred from mutant phenotype PubMed 17635913. Source: FlyBase

circadian rhythm

Non-traceable author statement PubMed 11715043. Source: FlyBase

eclosion rhythm

Non-traceable author statement PubMed 11715043. Source: FlyBase

locomotor rhythm

Non-traceable author statement PubMed 11715043. Source: FlyBase

negative regulation of transcription from RNA polymerase II promoter

Non-traceable author statement PubMed 11178250. Source: FlyBase

positive regulation of transcription from RNA polymerase II promoter

Non-traceable author statement PubMed 11178250. Source: FlyBase

positive regulation of transcription, DNA-templated

Non-traceable author statement PubMed 12015613. Source: FlyBase

regulation of circadian sleep/wake cycle, sleep

Inferred from mutant phenotype PubMed 12015603PubMed 16753559. Source: FlyBase

response to starvation

Inferred from direct assay PubMed 18840359. Source: FlyBase

rhythmic behavior

Traceable author statement PubMed 12486701. Source: FlyBase

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: InterPro

nucleus

Non-traceable author statement PubMed 12015613. Source: FlyBase

transcription factor complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionDNA binding

Inferred from direct assay PubMed 16543224. Source: FlyBase

protein binding

Inferred from physical interaction PubMed 19376119Ref.2. Source: IntAct

protein heterodimerization activity

Inferred from physical interaction PubMed 10409723PubMed 19376119. Source: FlyBase

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

signal transducer activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ClkO617352EBI-87683,EBI-143834

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 413413Protein cycle
PRO_0000127165

Regions

Domain30 – 8354bHLH
Domain104 – 17572PAS 1
Domain297 – 36771PAS 2
Domain372 – 41342PAC
Compositional bias228 – 2347Poly-Ser

Experimental info

Sequence conflict2421T → S in AAC39124. Ref.1
Sequence conflict2421T → S in AAD10629. Ref.2
Sequence conflict2421T → S in AAC62235. Ref.3
Sequence conflict2421T → S in AAM51144. Ref.6
Sequence conflict3081H → Y in AAC39124. Ref.1
Sequence conflict3621M → V in AAC39124. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O61734 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: 061C1769E6BBDB0D

FASTA41347,569
        10         20         30         40         50         60 
MEVQEFCENM EEIEDENYDE EKSARTSDEN RKQNHSEIEK RRRDKMNTYI NELSSMIPMC 

        70         80         90        100        110        120 
FAMQRKLDKL TVLRMAVQHL RGIRGSGSLH PFNGSDYRPS FLSDQELKMI ILQASEGFLF 

       130        140        150        160        170        180 
VVGCDRGRIL YVSDSVSSVL NSTQADLLGQ SWFDVLHPKD IGKVKEQLSS LEQCPRERLI 

       190        200        210        220        230        240 
DAKTMLPVKT DVPQSLCRLC PGARRSFFCR MKLRTASNNQ IKEESDTSSS SRSSTKRKSR 

       250        260        270        280        290        300 
LTTGHKYRVI QCTGYLKSWT PIKDEDQDAD SDEQTTNLSC LVAIGRIPPN VRNSTVPASL 

       310        320        330        340        350        360 
DNHPNIRHVL FISRHSGEGK FLFIDQRATL VIGFLPQEIL GTSFYEYFHN EDIAALMESH 

       370        380        390        400        410 
KMVMQVPEKV TTQVYRFRCK DNSYIQLQSE WRAFKNPWTS EIDYIIAKNS VFL 

« Hide

References

« Hide 'large scale' references
[1]"CYCLE is a second bHLH-PAS clock protein essential for circadian rhythmicity and transcription of Drosophila period and timeless."
Rutila J.E., Suri V., Le M., So W.V., Rosbash M., Hall J.C.
Cell 93:805-814(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE.
Tissue: Ovary.
[2]"Closing the circadian loop: CLOCK-induced transcription of its own inhibitors per and tim."
Darlington T.K., Wager-Smith K., Ceriani M.F., Staknis D., Gekakis N., Steeves T.D.L., Weitz C.J., Takahashi J.S., Kay S.A.
Science 280:1599-1603(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain and Muscle.
[3]Bae K.
Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[5]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[6]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
[7]Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF065473 mRNA. Translation: AAC39124.1.
AF067206 mRNA. Translation: AAD10629.1.
AF069998 mRNA. Translation: AAC62235.1.
AE014296 Genomic DNA. Translation: AAF49107.1.
AY119284 mRNA. Translation: AAM51144.1.
BT025197 mRNA. Translation: ABF17888.1.
RefSeqNP_524168.2. NM_079444.3.
UniGeneDm.1004.

3D structure databases

ProteinModelPortalO61734.
SMRO61734. Positions 29-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid65431. 2 interactions.
IntActO61734. 1 interaction.
MINTMINT-1328881.

Proteomic databases

PRIDEO61734.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0074924; FBpp0074693; FBgn0023094.
GeneID40162.
KEGGdme:Dmel_CG8727.

Organism-specific databases

CTD40162.
FlyBaseFBgn0023094. cyc.

Phylogenomic databases

eggNOGNOG293303.
GeneTreeENSGT00650000092935.
InParanoidO61734.
KOK02296.
OMAEQCPRER.
OrthoDBEOG7V1FQ8.
PhylomeDBO61734.

Gene expression databases

BgeeO61734.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
IPR001067. Nuc_translocat.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
[Graphical view]
PfamPF00010. HLH. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view]
PRINTSPR00785. NCTRNSLOCATR.
SMARTSM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 3 hits.
PROSITEPS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi40162.
NextBio817333.

Entry information

Entry nameCYCL_DROME
AccessionPrimary (citable) accession number: O61734
Secondary accession number(s): O76344 expand/collapse secondary AC list , Q1LZ29, Q540W7, Q9VW44
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 10, 2007
Last modified: July 9, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase