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O61734

- CYCL_DROME

UniProt

O61734 - CYCL_DROME

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Protein

Protein cycle

Gene

cyc

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Putative transcription factor involved in the generation of biological rhythms. Activates cycling transcription of Period (PER) and Timeless (TIM) by binding to the E-box (5'-CACGTG-3') present in their promoters.

GO - Molecular functioni

  1. DNA binding Source: FlyBase
  2. protein heterodimerization activity Source: FlyBase
  3. sequence-specific DNA binding transcription factor activity Source: InterPro
  4. signal transducer activity Source: InterPro

GO - Biological processi

  1. behavioral response to cocaine Source: FlyBase
  2. circadian regulation of gene expression Source: FlyBase
  3. circadian rhythm Source: FlyBase
  4. eclosion rhythm Source: FlyBase
  5. locomotor rhythm Source: FlyBase
  6. negative regulation of transcription from RNA polymerase II promoter Source: FlyBase
  7. positive regulation of transcription, DNA-templated Source: FlyBase
  8. positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  9. regulation of circadian sleep/wake cycle, sleep Source: FlyBase
  10. response to starvation Source: FlyBase
  11. rhythmic behavior Source: FlyBase
  12. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_180268. BMAL1:CLOCK,NPAS2 activates circadian gene expression.
REACT_180846. PPARA activates gene expression.
REACT_181316. RORA activates circadian gene expression.
REACT_181317. REV-ERBA represses gene expression.
REACT_239233. Circadian Clock.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein cycle
Alternative name(s):
Brain and muscle ARNT-like 1
Short name:
BMAL1
MOP3
Gene namesi
Name:cyc
ORF Names:CG8727
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0023094. cyc.

Subcellular locationi

Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  1. cytoplasm Source: InterPro
  2. nucleus Source: FlyBase
  3. transcription factor complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Cyc mutants don't display PER and TIM cycling, and are completely arrhythmic.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 413413Protein cyclePRO_0000127165Add
BLAST

Proteomic databases

PRIDEiO61734.

Expressioni

Tissue specificityi

Expressed in head and ovary.

Gene expression databases

BgeeiO61734.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with Clock in order to activate PER and TIM transcription.

Binary interactionsi

WithEntry#Exp.IntActNotes
ClkO617352EBI-87683,EBI-143834

Protein-protein interaction databases

BioGridi65431. 2 interactions.
IntActiO61734. 1 interaction.
MINTiMINT-1328881.

Structurei

3D structure databases

ProteinModelPortaliO61734.
SMRiO61734. Positions 29-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 8354bHLHPROSITE-ProRule annotationAdd
BLAST
Domaini104 – 17572PAS 1PROSITE-ProRule annotationAdd
BLAST
Domaini297 – 36771PAS 2PROSITE-ProRule annotationAdd
BLAST
Domaini372 – 41342PACAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi228 – 2347Poly-Ser

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 2 PAS (PER-ARNT-SIM) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG293303.
GeneTreeiENSGT00760000118788.
InParanoidiO61734.
KOiK02296.
OMAiEQCPRER.
OrthoDBiEOG7V1FQ8.
PhylomeDBiO61734.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR001067. Nuc_translocat.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view]
PRINTSiPR00785. NCTRNSLOCATR.
SMARTiSM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 3 hits.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O61734-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEVQEFCENM EEIEDENYDE EKSARTSDEN RKQNHSEIEK RRRDKMNTYI
60 70 80 90 100
NELSSMIPMC FAMQRKLDKL TVLRMAVQHL RGIRGSGSLH PFNGSDYRPS
110 120 130 140 150
FLSDQELKMI ILQASEGFLF VVGCDRGRIL YVSDSVSSVL NSTQADLLGQ
160 170 180 190 200
SWFDVLHPKD IGKVKEQLSS LEQCPRERLI DAKTMLPVKT DVPQSLCRLC
210 220 230 240 250
PGARRSFFCR MKLRTASNNQ IKEESDTSSS SRSSTKRKSR LTTGHKYRVI
260 270 280 290 300
QCTGYLKSWT PIKDEDQDAD SDEQTTNLSC LVAIGRIPPN VRNSTVPASL
310 320 330 340 350
DNHPNIRHVL FISRHSGEGK FLFIDQRATL VIGFLPQEIL GTSFYEYFHN
360 370 380 390 400
EDIAALMESH KMVMQVPEKV TTQVYRFRCK DNSYIQLQSE WRAFKNPWTS
410
EIDYIIAKNS VFL
Length:413
Mass (Da):47,569
Last modified:July 10, 2007 - v2
Checksum:i061C1769E6BBDB0D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti242 – 2421T → S in AAC39124. (PubMed:9630224)Curated
Sequence conflicti242 – 2421T → S in AAD10629. (PubMed:9616122)Curated
Sequence conflicti242 – 2421T → S in AAC62235. 1 PublicationCurated
Sequence conflicti242 – 2421T → S in AAM51144. (PubMed:12537569)Curated
Sequence conflicti308 – 3081H → Y in AAC39124. (PubMed:9630224)Curated
Sequence conflicti362 – 3621M → V in AAC39124. (PubMed:9630224)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF065473 mRNA. Translation: AAC39124.1.
AF067206 mRNA. Translation: AAD10629.1.
AF069998 mRNA. Translation: AAC62235.1.
AE014296 Genomic DNA. Translation: AAF49107.1.
AY119284 mRNA. Translation: AAM51144.1.
BT025197 mRNA. Translation: ABF17888.1.
RefSeqiNP_524168.2. NM_079444.3.
UniGeneiDm.1004.

Genome annotation databases

EnsemblMetazoaiFBtr0074924; FBpp0074693; FBgn0023094.
GeneIDi40162.
KEGGidme:Dmel_CG8727.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF065473 mRNA. Translation: AAC39124.1 .
AF067206 mRNA. Translation: AAD10629.1 .
AF069998 mRNA. Translation: AAC62235.1 .
AE014296 Genomic DNA. Translation: AAF49107.1 .
AY119284 mRNA. Translation: AAM51144.1 .
BT025197 mRNA. Translation: ABF17888.1 .
RefSeqi NP_524168.2. NM_079444.3.
UniGenei Dm.1004.

3D structure databases

ProteinModelPortali O61734.
SMRi O61734. Positions 29-413.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 65431. 2 interactions.
IntActi O61734. 1 interaction.
MINTi MINT-1328881.

Proteomic databases

PRIDEi O61734.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0074924 ; FBpp0074693 ; FBgn0023094 .
GeneIDi 40162.
KEGGi dme:Dmel_CG8727.

Organism-specific databases

CTDi 40162.
FlyBasei FBgn0023094. cyc.

Phylogenomic databases

eggNOGi NOG293303.
GeneTreei ENSGT00760000118788.
InParanoidi O61734.
KOi K02296.
OMAi EQCPRER.
OrthoDBi EOG7V1FQ8.
PhylomeDBi O61734.

Enzyme and pathway databases

Reactomei REACT_180268. BMAL1:CLOCK,NPAS2 activates circadian gene expression.
REACT_180846. PPARA activates gene expression.
REACT_181316. RORA activates circadian gene expression.
REACT_181317. REV-ERBA represses gene expression.
REACT_239233. Circadian Clock.

Miscellaneous databases

GenomeRNAii 40162.
NextBioi 817333.

Gene expression databases

Bgeei O61734.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
IPR001067. Nuc_translocat.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
[Graphical view ]
Pfami PF00010. HLH. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view ]
PRINTSi PR00785. NCTRNSLOCATR.
SMARTi SM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 3 hits.
PROSITEi PS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "CYCLE is a second bHLH-PAS clock protein essential for circadian rhythmicity and transcription of Drosophila period and timeless."
    Rutila J.E., Suri V., Le M., So W.V., Rosbash M., Hall J.C.
    Cell 93:805-814(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE.
    Tissue: Ovary.
  2. "Closing the circadian loop: CLOCK-induced transcription of its own inhibitors per and tim."
    Darlington T.K., Wager-Smith K., Ceriani M.F., Staknis D., Gekakis N., Steeves T.D.L., Weitz C.J., Takahashi J.S., Kay S.A.
    Science 280:1599-1603(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain and Muscle.
  3. Bae K.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  5. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  7. Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.

Entry informationi

Entry nameiCYCL_DROME
AccessioniPrimary (citable) accession number: O61734
Secondary accession number(s): O76344
, Q1LZ29, Q540W7, Q9VW44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 10, 2007
Last modified: November 26, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3