Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein cycle

Gene

cyc

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Putative transcription factor involved in the generation of biological rhythms. Activates cycling transcription of Period (PER) and Timeless (TIM) by binding to the E-box (5'-CACGTG-3') present in their promoters.

GO - Molecular functioni

  • DNA binding Source: FlyBase
  • DNA binding transcription factor activity Source: InterPro
  • protein heterodimerization activity Source: FlyBase

GO - Biological processi

  • behavioral response to cocaine Source: FlyBase
  • circadian regulation of gene expression Source: FlyBase
  • circadian regulation of heart rate Source: FlyBase
  • circadian rhythm Source: FlyBase
  • eclosion rhythm Source: FlyBase
  • locomotor rhythm Source: FlyBase
  • negative regulation of transcription by RNA polymerase II Source: FlyBase
  • positive regulation of transcription, DNA-templated Source: FlyBase
  • positive regulation of transcription by RNA polymerase II Source: FlyBase
  • regulation of circadian sleep/wake cycle, sleep Source: FlyBase
  • response to starvation Source: FlyBase
  • rhythmic behavior Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processBiological rhythms, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-432395 Degradation of TIM
R-DME-432408 Transcription regulation of cwo gene
R-DME-432501 Transcription repression by PER and activation by PDP1
R-DME-432524 Degradation of PER
R-DME-432560 Transcription activation by CLK:CYC and repression by VRI
R-DME-432620 Dephosphorylation of PER
R-DME-538848 Degradation of CLK
R-DME-538909 Dephosphorylation of CLK

Names & Taxonomyi

Protein namesi
Recommended name:
Protein cycle
Alternative name(s):
Brain and muscle ARNT-like 1
Short name:
BMAL1
MOP3
Gene namesi
Name:cyc
ORF Names:CG8727
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0023094 cyc

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Cyc mutants don't display PER and TIM cycling, and are completely arrhythmic.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001271651 – 413Protein cycleAdd BLAST413

Proteomic databases

PaxDbiO61734
PRIDEiO61734

Expressioni

Tissue specificityi

Expressed in head and ovary.

Gene expression databases

BgeeiFBgn0023094
GenevisibleiO61734 DM

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with Clock in order to activate PER and TIM transcription.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei35Interaction with E-box DNABy similarity1
Sitei38Interaction with E-box DNABy similarity1
Sitei39Interaction with E-box DNABy similarity1
Sitei43Interaction with E-box DNABy similarity1

Binary interactionsi

WithEntry#Exp.IntActNotes
ClkO617352EBI-87683,EBI-143834

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi65431, 6 interactors
IntActiO61734, 3 interactors
MINTiO61734
STRINGi7227.FBpp0074693

Structurei

Secondary structure

1413
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi311 – 315Combined sources5
Beta strandi320 – 324Combined sources5
Helixi328 – 332Combined sources5
Helixi336 – 339Combined sources4
Helixi344 – 346Combined sources3
Turni350 – 352Combined sources3
Helixi353 – 365Combined sources13
Beta strandi366 – 368Combined sources3
Beta strandi375 – 378Combined sources4
Beta strandi384 – 395Combined sources12
Turni397 – 399Combined sources3
Beta strandi402 – 411Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5F5YX-ray2.20A311-412[»]
5F68X-ray1.23A311-412[»]
5F69X-ray1.37A311-412[»]
5F6AX-ray1.93A311-412[»]
ProteinModelPortaliO61734
SMRiO61734
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 83bHLHPROSITE-ProRule annotationAdd BLAST54
Domaini104 – 175PAS 1PROSITE-ProRule annotationAdd BLAST72
Domaini297 – 367PAS 2PROSITE-ProRule annotationAdd BLAST71
Domaini372 – 413PACAdd BLAST42

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi228 – 234Poly-Ser7

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3561 Eukaryota
ENOG410XRJI LUCA
GeneTreeiENSGT00760000118788
InParanoidiO61734
KOiK02296
OMAiFFCRMKL
OrthoDBiEOG091G126J
PhylomeDBiO61734

Family and domain databases

CDDicd00083 HLH, 1 hit
cd00130 PAS, 2 hits
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR001067 Nuc_translocat
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
PfamiView protein in Pfam
PF00010 HLH, 1 hit
PF00989 PAS, 1 hit
PRINTSiPR00785 NCTRNSLOCATR
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SM00091 PAS, 2 hits
SUPFAMiSSF47459 SSF47459, 1 hit
SSF55785 SSF55785, 3 hits
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS50112 PAS, 2 hits

Sequencei

Sequence statusi: Complete.

O61734-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVQEFCENM EEIEDENYDE EKSARTSDEN RKQNHSEIEK RRRDKMNTYI
60 70 80 90 100
NELSSMIPMC FAMQRKLDKL TVLRMAVQHL RGIRGSGSLH PFNGSDYRPS
110 120 130 140 150
FLSDQELKMI ILQASEGFLF VVGCDRGRIL YVSDSVSSVL NSTQADLLGQ
160 170 180 190 200
SWFDVLHPKD IGKVKEQLSS LEQCPRERLI DAKTMLPVKT DVPQSLCRLC
210 220 230 240 250
PGARRSFFCR MKLRTASNNQ IKEESDTSSS SRSSTKRKSR LTTGHKYRVI
260 270 280 290 300
QCTGYLKSWT PIKDEDQDAD SDEQTTNLSC LVAIGRIPPN VRNSTVPASL
310 320 330 340 350
DNHPNIRHVL FISRHSGEGK FLFIDQRATL VIGFLPQEIL GTSFYEYFHN
360 370 380 390 400
EDIAALMESH KMVMQVPEKV TTQVYRFRCK DNSYIQLQSE WRAFKNPWTS
410
EIDYIIAKNS VFL
Length:413
Mass (Da):47,569
Last modified:July 10, 2007 - v2
Checksum:i061C1769E6BBDB0D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti242T → S in AAC39124 (PubMed:9630224).Curated1
Sequence conflicti242T → S in AAD10629 (PubMed:9616122).Curated1
Sequence conflicti242T → S in AAC62235 (Ref. 3) Curated1
Sequence conflicti242T → S in AAM51144 (PubMed:12537569).Curated1
Sequence conflicti308H → Y in AAC39124 (PubMed:9630224).Curated1
Sequence conflicti362M → V in AAC39124 (PubMed:9630224).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF065473 mRNA Translation: AAC39124.1
AF067206 mRNA Translation: AAD10629.1
AF069998 mRNA Translation: AAC62235.1
AE014296 Genomic DNA Translation: AAF49107.1
AY119284 mRNA Translation: AAM51144.1
BT025197 mRNA Translation: ABF17888.1
RefSeqiNP_524168.2, NM_079444.3
UniGeneiDm.1004

Genome annotation databases

EnsemblMetazoaiFBtr0074924; FBpp0074693; FBgn0023094
GeneIDi40162
KEGGidme:Dmel_CG8727

Similar proteinsi

Entry informationi

Entry nameiCYCL_DROME
AccessioniPrimary (citable) accession number: O61734
Secondary accession number(s): O76344
, Q1LZ29, Q540W7, Q9VW44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 10, 2007
Last modified: May 23, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health