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O61734

- CYCL_DROME

UniProt

O61734 - CYCL_DROME

Protein

Protein cycle

Gene

cyc

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 2 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Putative transcription factor involved in the generation of biological rhythms. Activates cycling transcription of Period (PER) and Timeless (TIM) by binding to the E-box (5'-CACGTG-3') present in their promoters.

    GO - Molecular functioni

    1. DNA binding Source: FlyBase
    2. protein binding Source: IntAct
    3. protein heterodimerization activity Source: FlyBase
    4. sequence-specific DNA binding transcription factor activity Source: InterPro
    5. signal transducer activity Source: InterPro

    GO - Biological processi

    1. behavioral response to cocaine Source: FlyBase
    2. circadian regulation of gene expression Source: FlyBase
    3. circadian rhythm Source: FlyBase
    4. eclosion rhythm Source: FlyBase
    5. locomotor rhythm Source: FlyBase
    6. negative regulation of transcription from RNA polymerase II promoter Source: FlyBase
    7. positive regulation of transcription, DNA-templated Source: FlyBase
    8. positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
    9. regulation of circadian sleep/wake cycle, sleep Source: FlyBase
    10. response to starvation Source: FlyBase
    11. rhythmic behavior Source: FlyBase
    12. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Biological rhythms, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_180268. BMAL1:CLOCK,NPAS2 activates circadian gene expression.
    REACT_181316. RORA activates circadian gene expression.
    REACT_181317. REV-ERBA represses gene expression.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein cycle
    Alternative name(s):
    Brain and muscle ARNT-like 1
    Short name:
    BMAL1
    MOP3
    Gene namesi
    Name:cyc
    ORF Names:CG8727
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3L

    Organism-specific databases

    FlyBaseiFBgn0023094. cyc.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: InterPro
    2. nucleus Source: FlyBase
    3. transcription factor complex Source: InterPro

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Cyc mutants don't display PER and TIM cycling, and are completely arrhythmic.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 413413Protein cyclePRO_0000127165Add
    BLAST

    Proteomic databases

    PRIDEiO61734.

    Expressioni

    Tissue specificityi

    Expressed in head and ovary.

    Gene expression databases

    BgeeiO61734.

    Interactioni

    Subunit structurei

    Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with Clock in order to activate PER and TIM transcription.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ClkO617352EBI-87683,EBI-143834

    Protein-protein interaction databases

    BioGridi65431. 2 interactions.
    IntActiO61734. 1 interaction.
    MINTiMINT-1328881.

    Structurei

    3D structure databases

    ProteinModelPortaliO61734.
    SMRiO61734. Positions 29-413.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 8354bHLHPROSITE-ProRule annotationAdd
    BLAST
    Domaini104 – 17572PAS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini297 – 36771PAS 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini372 – 41342PACAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi228 – 2347Poly-Ser

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
    Contains 2 PAS (PER-ARNT-SIM) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG293303.
    GeneTreeiENSGT00650000092935.
    InParanoidiO61734.
    KOiK02296.
    OMAiEQCPRER.
    OrthoDBiEOG7V1FQ8.
    PhylomeDBiO61734.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    IPR001067. Nuc_translocat.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR013767. PAS_fold.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    PF00989. PAS. 1 hit.
    [Graphical view]
    PRINTSiPR00785. NCTRNSLOCATR.
    SMARTiSM00353. HLH. 1 hit.
    SM00086. PAC. 1 hit.
    SM00091. PAS. 2 hits.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    SSF55785. SSF55785. 3 hits.
    PROSITEiPS50888. BHLH. 1 hit.
    PS50112. PAS. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O61734-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVQEFCENM EEIEDENYDE EKSARTSDEN RKQNHSEIEK RRRDKMNTYI    50
    NELSSMIPMC FAMQRKLDKL TVLRMAVQHL RGIRGSGSLH PFNGSDYRPS 100
    FLSDQELKMI ILQASEGFLF VVGCDRGRIL YVSDSVSSVL NSTQADLLGQ 150
    SWFDVLHPKD IGKVKEQLSS LEQCPRERLI DAKTMLPVKT DVPQSLCRLC 200
    PGARRSFFCR MKLRTASNNQ IKEESDTSSS SRSSTKRKSR LTTGHKYRVI 250
    QCTGYLKSWT PIKDEDQDAD SDEQTTNLSC LVAIGRIPPN VRNSTVPASL 300
    DNHPNIRHVL FISRHSGEGK FLFIDQRATL VIGFLPQEIL GTSFYEYFHN 350
    EDIAALMESH KMVMQVPEKV TTQVYRFRCK DNSYIQLQSE WRAFKNPWTS 400
    EIDYIIAKNS VFL 413
    Length:413
    Mass (Da):47,569
    Last modified:July 10, 2007 - v2
    Checksum:i061C1769E6BBDB0D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti242 – 2421T → S in AAC39124. (PubMed:9630224)Curated
    Sequence conflicti242 – 2421T → S in AAD10629. (PubMed:9616122)Curated
    Sequence conflicti242 – 2421T → S in AAC62235. 1 PublicationCurated
    Sequence conflicti242 – 2421T → S in AAM51144. (PubMed:12537569)Curated
    Sequence conflicti308 – 3081H → Y in AAC39124. (PubMed:9630224)Curated
    Sequence conflicti362 – 3621M → V in AAC39124. (PubMed:9630224)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF065473 mRNA. Translation: AAC39124.1.
    AF067206 mRNA. Translation: AAD10629.1.
    AF069998 mRNA. Translation: AAC62235.1.
    AE014296 Genomic DNA. Translation: AAF49107.1.
    AY119284 mRNA. Translation: AAM51144.1.
    BT025197 mRNA. Translation: ABF17888.1.
    RefSeqiNP_524168.2. NM_079444.3.
    UniGeneiDm.1004.

    Genome annotation databases

    EnsemblMetazoaiFBtr0074924; FBpp0074693; FBgn0023094.
    GeneIDi40162.
    KEGGidme:Dmel_CG8727.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF065473 mRNA. Translation: AAC39124.1 .
    AF067206 mRNA. Translation: AAD10629.1 .
    AF069998 mRNA. Translation: AAC62235.1 .
    AE014296 Genomic DNA. Translation: AAF49107.1 .
    AY119284 mRNA. Translation: AAM51144.1 .
    BT025197 mRNA. Translation: ABF17888.1 .
    RefSeqi NP_524168.2. NM_079444.3.
    UniGenei Dm.1004.

    3D structure databases

    ProteinModelPortali O61734.
    SMRi O61734. Positions 29-413.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 65431. 2 interactions.
    IntActi O61734. 1 interaction.
    MINTi MINT-1328881.

    Proteomic databases

    PRIDEi O61734.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0074924 ; FBpp0074693 ; FBgn0023094 .
    GeneIDi 40162.
    KEGGi dme:Dmel_CG8727.

    Organism-specific databases

    CTDi 40162.
    FlyBasei FBgn0023094. cyc.

    Phylogenomic databases

    eggNOGi NOG293303.
    GeneTreei ENSGT00650000092935.
    InParanoidi O61734.
    KOi K02296.
    OMAi EQCPRER.
    OrthoDBi EOG7V1FQ8.
    PhylomeDBi O61734.

    Enzyme and pathway databases

    Reactomei REACT_180268. BMAL1:CLOCK,NPAS2 activates circadian gene expression.
    REACT_181316. RORA activates circadian gene expression.
    REACT_181317. REV-ERBA represses gene expression.

    Miscellaneous databases

    GenomeRNAii 40162.
    NextBioi 817333.

    Gene expression databases

    Bgeei O61734.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    IPR001067. Nuc_translocat.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR013767. PAS_fold.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    PF00989. PAS. 1 hit.
    [Graphical view ]
    PRINTSi PR00785. NCTRNSLOCATR.
    SMARTi SM00353. HLH. 1 hit.
    SM00086. PAC. 1 hit.
    SM00091. PAS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    SSF55785. SSF55785. 3 hits.
    PROSITEi PS50888. BHLH. 1 hit.
    PS50112. PAS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CYCLE is a second bHLH-PAS clock protein essential for circadian rhythmicity and transcription of Drosophila period and timeless."
      Rutila J.E., Suri V., Le M., So W.V., Rosbash M., Hall J.C.
      Cell 93:805-814(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE.
      Tissue: Ovary.
    2. "Closing the circadian loop: CLOCK-induced transcription of its own inhibitors per and tim."
      Darlington T.K., Wager-Smith K., Ceriani M.F., Staknis D., Gekakis N., Steeves T.D.L., Weitz C.J., Takahashi J.S., Kay S.A.
      Science 280:1599-1603(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain and Muscle.
    3. Bae K.
      Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    5. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.
    7. Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.

    Entry informationi

    Entry nameiCYCL_DROME
    AccessioniPrimary (citable) accession number: O61734
    Secondary accession number(s): O76344
    , Q1LZ29, Q540W7, Q9VW44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 133 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3