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O61705 (SCXA_MESMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Neurotoxin BmK-M10
Alternative name(s):
Alpha-neurotoxin TX9
BmK AngM1
BmK-X
Short name=BmKX
BmK10
BmKalphaTx9
Bmk M10
Short name=BmkM10
Neurotoxin M10
OrganismMesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Taxonomic identifier34649 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Ecdysozoa › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Mesobuthus

Protein attributes

Sequence length84 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to voltage-dependent sodium channels (Nav) and voltage-dependent delayed rectifier potassium channels and inhibits the inactivation of the activated channels, thereby blocking neuronal transmission. Administration to mice at a dosage of 0.8 mg/kg produces an analgesic effect. Ref.3

Subcellular location

Secreted Ref.3.

Tissue specificity

Expressed by the venom gland. Ref.3

Sequence similarities

Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Alpha subfamily.

Mass spectrometry

Molecular mass is 7040.5 Da from positions 20 - 83. Determined by ESI. Ref.3

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionIon channel impairing toxin
Neurotoxin
Sodium channel inhibitor
Toxin
   PTMDisulfide bond
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processdefense response

Inferred from electronic annotation. Source: InterPro

pathogenesis

Inferred from direct assay Ref.3. Source: UniProtKB

   Cellular_componentextracellular region

Inferred from direct assay Ref.3. Source: UniProtKB

   Molecular_functionpotassium channel inhibitor activity

Inferred from direct assay Ref.3. Source: UniProtKB

sodium channel inhibitor activity

Inferred from direct assay Ref.3. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Ref.3
Chain20 – 8364Neurotoxin BmK-M10
PRO_0000035240
Propeptide841Removed by a carboxypeptidase
PRO_0000035241

Amino acid modifications

Disulfide bond31 ↔ 82 By similarity
Disulfide bond35 ↔ 55 By similarity
Disulfide bond41 ↔ 65 By similarity
Disulfide bond45 ↔ 67 By similarity

Secondary structure

1 ............. 84
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O61705 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 62D3FD255F7E2D61

FASTA849,346
        10         20         30         40         50         60 
MNYLVMISFA LLLMKGVESV RDAYIAKPEN CVYECGITQD CNKLCTENGA ESGYCQWGGK 

        70         80 
YGNACWCIKL PDSVPIRVPG KCQR

« Hide

References

[1]Xiong Y.-M., Ling M.-H., Wang D.-C., Chi C.-W.
Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Venom gland.
[2]"Nine novel precursors of Buthus martensii scorpion alpha-toxin homologues."
Zhu S.-Y., Li W.-X., Zeng X.-C., Liu H., Jiang D.-H., Mao X.
Toxicon 38:1653-1661(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Venom gland.
[3]"Purification and characterization of a new peptide with analgesic effect from the scorpion Buthus martensi Karch."
Cao Z.Y., Mi Z.M., Cheng G.F., Shen W.Q., Xiao X., Liu X.M., Liang X.T., Yu D.Q.
J. Pept. Res. 64:33-41(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-83, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF062563 Genomic DNA. Translation: AAC16697.1.
AF164203 mRNA. Translation: AAK06898.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2KBKNMR-A20-83[»]
ProteinModelPortalO61705.
SMRO61705. Positions 20-83.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.30.10. 1 hit.
InterProIPR003614. Scorpion_toxin-like.
IPR018218. Scorpion_toxinL.
IPR002061. Scorpion_toxinL/defesin.
[Graphical view]
PfamPF00537. Toxin_3. 1 hit.
[Graphical view]
PRINTSPR00285. SCORPNTOXIN.
SMARTSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMSSF57095. SSF57095. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceO61705.

Entry information

Entry nameSCXA_MESMA
AccessionPrimary (citable) accession number: O61705
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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