O61667 (EGL1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Programmed cell death activator egl-1

Alternative name(s):
Egg-laying defective protein 1

Gene names

Name:	egl-1
ORF Names:	F23B12.9

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	106 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a major role in programmed cell death (PCD or apoptosis) by negatively regulating ced-9. Binds to and directly inhibits the activity of ced-9, releasing the cell death activator ced-4 from a ced-9/ced-4 containing protein complex and allowing ced-4 to activate the cell-killing caspase ced-3. Ref.1 Ref.3 Ref.4
Disruption phenotype	Mutants block programmed cell death. Ref.1
Sequence caution	The sequence AAC39023.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Apoptosis
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic mitochondrial changes Inferred from mutant phenotype. Source: WormBase negative regulation of anti-apoptosis Inferred from mutant phenotype. Source: WormBase negative regulation of protein binding Inferred from direct assay. Source: WormBase negative regulation of survival gene product expression Inferred from direct assay. Source: WormBase oviposition Inferred from mutant phenotype Ref.1. Source: WormBase
Cellular component	cytosol Inferred from direct assay. Source: WormBase mitochondrion Inferred from direct assay. Source: WormBase
Molecular function	protein binding Inferred from physical interaction Ref.4. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ced-9	P41958	6	EBI-495949,EBI-494110

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 106

106

Programmed cell death activator egl-1

PRO_0000086944

Secondary structure

1

.

.

.

.

106

Helix Strand Turn

Details...

Helix

65 – 84

20

Helix

85 – 87

3

Sequences

	Sequence		Length	Mass (Da)
	O61667 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 2329D2DB0F019636	FASTA	106	11,916
	10 20 30 40 50 60 MLMLTFASTS SDLLPMSNVF DVQSSVFYNE KNMFYSSSQD FSSCEDSSQF ADDSGFFDDS 70 80 90 100 EISSIGYEIG SKLAAMCDDF DAQMMSYSAH ASDRSLFHRL LDFFAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The C. elegans protein EGL-1 is required for programmed cell death and interacts with the Bcl-2-like protein CED-9." Conradt B., Horvitz H.R. Cell 93:519-529(1998) [PubMed: 9604928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CED-9, DISRUPTION PHENOTYPE. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Translocation of C. elegans CED-4 to nuclear membranes during programmed cell death." Chen F., Hersh B.M., Conradt B., Zhou Z., Riemer D., Gruenbaum Y., Horvitz H.R. Science 287:1485-1489(2000) [PubMed: 10688797] [Abstract] Cited for: FUNCTION.
[4]	"Structural, biochemical, and functional analyses of CED-9 recognition by the proapoptotic proteins EGL-1 and CED-4." Yan N., Gu L., Kokel D., Chai J., Li W., Han A., Chen L., Xue D., Shi Y. Mol. Cell 15:999-1006(2004) [PubMed: 15383288] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 46-102 IN COMPLEX WITH CED-9, FUNCTION, INTERACTION WITH CED-9.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF057309 mRNA. Translation: AAC39023.1. Different initiation.
Z77659 Genomic DNA. Translation: CAB82213.2.

PIR

T43032.

RefSeq

NP_506575.2. NM_074174.2.

UniGene

Cel.19641.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TY4	X-ray	2.20	C/D	46-102	[»]

ProteinModelPortal

O61667.

SMR

O61667. Positions 63-91.

ModBase

Search...

Protein-protein interaction databases

IntAct

O61667. 1 interaction.

STRING

O61667.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

F23B12.9; F23B12.9; F23B12.9.

GeneID

179943.

KEGG

cel:F23B12.9.

NMPDR

fig|6239.3.peg.20588.

UCSC

F23B12.9. c. elegans.

Organism-specific databases

CTD

179943.

WormBase

F23B12.9; CE35714; WBGene00001170; egl-1.

Phylogenomic databases

GeneTree

EMGT00050000019725.

InParanoid

O61667.

OMA

DAEMMSY.

Gene expression databases

ArrayExpress

O61667.

Family and domain databases

InterPro

IPR021543. EGL-1.
[Graphical view]

Pfam

PF11430. EGL-1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

907490.

Entry information

Entry name

EGL1_CAEEL

Accession

Primary (citable) accession number: O61667
Secondary accession number(s): Q7JLC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	September 21, 2011
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project