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O61660 (TOP3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 3

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase III
Gene names
Name:top-3
ORF Names:Y56A3A.27
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length759 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 759759DNA topoisomerase 3
PRO_0000145194

Regions

Domain3 – 147145Toprim

Sites

Active site3341O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
O61660 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 3D862412D72946BD

FASTA75985,438
        10         20         30         40         50         60 
MKRALFVAEK NDVAKGVAAI LSNGTANRRE GRSKFNKIYT LNTELFGQQT AISVTSVSGH 

        70         80         90        100        110        120 
MMNFQFHENM SNWQTASMVE LFRAPVRHVV TPEMKLIEQT LREQAQRHDI LVVWTDCDRE 

       130        140        150        160        170        180 
GEAIGAEIVK VCRDSNRRLD IFRARFSEIT KAAITRAARN LIRLDEKTVA AVDCRSELDL 

       190        200        210        220        230        240 
RIGSAFTRLQ TLHLRNRFRD LLGQNDTSQV ISYGSCQFPT LGFVTDRYKM IENFVSEPFW 

       250        260        270        280        290        300 
KLIVEHTRES HKVEFLWDRN RLFDRDTVDI LHDECKETKE AHVEKVAKKP KSKWRPQALD 

       310        320        330        340        350        360 
TVELEKLGIS KLRMSAKQTM QVAEKLYSKG FISYPRTETN KFPAGLNLTP LVQQQTQSNI 

       370        380        390        400        410        420 
WGDFANEVLQ NGVNPRNGRK SDEAHPPIHP LKFTEKHQLQ GDDWKVYELV VRHFLACVSQ 

       430        440        450        460        470        480 
DAQGEETMVN LTVGTEKFHA SGLRIRDMGY LKVYVYEKWG NRLLPTYTEG ERFTDFELKI 

       490        500        510        520        530        540 
GDGKTQAPDF LTEADLISLM DKYGIGTDAT HAEHIEKIKT REYIGVRPDG KLIPSFLGLA 

       550        560        570        580        590        600 
LVDGYDDMGF AMSKPDLRAN LEIGLKEICD GRRQKQEVLD EQIGKYRAIF VESERKIGVL 

       610        620        630        640        650        660 
SQSLQRYLDK NNQAGGGPGG PGGGGGPPRG PGGGGGGGPT GPPAPPKPPA KPRGRPPRKS 

       670        680        690        700        710        720 
ISPAVKNGHD DPENDTIVTL SEVFGSMSNP KPARKPRAPR KSAAPKEQEE EEEVFCQCPE 

       730        740        750 
PMRAVTKVVQ KEGPNKGKKF YTCSLPYTSS EKCNFFKWA 

« Hide

References

« Hide 'large scale' references
[1]"Functional characterization of Caenorhabditis elegans DNA topoisomerase IIIalpha."
Kim Y.-C., Lee J., Koo H.-S.
Nucleic Acids Res. 28:2012-2017(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF057032 mRNA. Translation: AAC13567.1.
AL132860 Genomic DNA. Translation: CAB60518.2.
PIRT43031.
RefSeqNP_499558.1. NM_067157.4.
UniGeneCel.19512.

3D structure databases

ProteinModelPortalO61660.
SMRO61660. Positions 5-595.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6239.Y56A3A.27.2.

Proteomic databases

PaxDbO61660.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaY56A3A.27.1; Y56A3A.27.1; Y56A3A.27.
Y56A3A.27.2; Y56A3A.27.2; Y56A3A.27.
GeneID176631.
KEGGcel:CELE_Y56A3A.27.
UCSCY56A3A.27.1. c. elegans.

Organism-specific databases

CTD176631.
WormBaseY56A3A.27; CE28138; WBGene00006596; top-3.

Phylogenomic databases

eggNOGCOG0550.
HOGENOMHOG000184377.
InParanoidO61660.
KOK03165.
OMANFKPETF.
PhylomeDBO61660.

Family and domain databases

Gene3D1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR006171. Toprim_domain.
IPR010666. Znf_GRF.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF06839. zf-GRF. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio893376.
PROO61660.

Entry information

Entry nameTOP3_CAEEL
AccessionPrimary (citable) accession number: O61660
Secondary accession number(s): Q9U223
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase