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O61660

- TOP3_CAEEL

UniProt

O61660 - TOP3_CAEEL

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Protein

DNA topoisomerase 3

Gene

top-3

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei334 – 3341O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-EC
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase III
Gene namesi
Name:top-3
ORF Names:Y56A3A.27
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiY56A3A.27; CE28138; WBGene00006596; top-3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 759759DNA topoisomerase 3PRO_0000145194Add
BLAST

Proteomic databases

PaxDbiO61660.

Interactioni

Protein-protein interaction databases

STRINGi6239.Y56A3A.27.2.

Structurei

3D structure databases

ProteinModelPortaliO61660.
SMRiO61660. Positions 1-608.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 147145ToprimPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the type IA topoisomerase family.Curated
Contains 1 Toprim domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0550.
GeneTreeiENSGT00530000063430.
HOGENOMiHOG000184377.
InParanoidiO61660.
KOiK03165.
OMAiVVILYER.
PhylomeDBiO61660.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR006171. Toprim_domain.
IPR010666. Znf_GRF.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF06839. zf-GRF. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O61660-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRALFVAEK NDVAKGVAAI LSNGTANRRE GRSKFNKIYT LNTELFGQQT
60 70 80 90 100
AISVTSVSGH MMNFQFHENM SNWQTASMVE LFRAPVRHVV TPEMKLIEQT
110 120 130 140 150
LREQAQRHDI LVVWTDCDRE GEAIGAEIVK VCRDSNRRLD IFRARFSEIT
160 170 180 190 200
KAAITRAARN LIRLDEKTVA AVDCRSELDL RIGSAFTRLQ TLHLRNRFRD
210 220 230 240 250
LLGQNDTSQV ISYGSCQFPT LGFVTDRYKM IENFVSEPFW KLIVEHTRES
260 270 280 290 300
HKVEFLWDRN RLFDRDTVDI LHDECKETKE AHVEKVAKKP KSKWRPQALD
310 320 330 340 350
TVELEKLGIS KLRMSAKQTM QVAEKLYSKG FISYPRTETN KFPAGLNLTP
360 370 380 390 400
LVQQQTQSNI WGDFANEVLQ NGVNPRNGRK SDEAHPPIHP LKFTEKHQLQ
410 420 430 440 450
GDDWKVYELV VRHFLACVSQ DAQGEETMVN LTVGTEKFHA SGLRIRDMGY
460 470 480 490 500
LKVYVYEKWG NRLLPTYTEG ERFTDFELKI GDGKTQAPDF LTEADLISLM
510 520 530 540 550
DKYGIGTDAT HAEHIEKIKT REYIGVRPDG KLIPSFLGLA LVDGYDDMGF
560 570 580 590 600
AMSKPDLRAN LEIGLKEICD GRRQKQEVLD EQIGKYRAIF VESERKIGVL
610 620 630 640 650
SQSLQRYLDK NNQAGGGPGG PGGGGGPPRG PGGGGGGGPT GPPAPPKPPA
660 670 680 690 700
KPRGRPPRKS ISPAVKNGHD DPENDTIVTL SEVFGSMSNP KPARKPRAPR
710 720 730 740 750
KSAAPKEQEE EEEVFCQCPE PMRAVTKVVQ KEGPNKGKKF YTCSLPYTSS

EKCNFFKWA
Length:759
Mass (Da):85,438
Last modified:August 1, 1998 - v1
Checksum:i3D862412D72946BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057032 mRNA. Translation: AAC13567.1.
AL132860 Genomic DNA. Translation: CAB60518.2.
PIRiT43031.
RefSeqiNP_499558.1. NM_067157.4.
UniGeneiCel.19512.

Genome annotation databases

EnsemblMetazoaiY56A3A.27; Y56A3A.27; WBGene00006596.
GeneIDi176631.
KEGGicel:CELE_Y56A3A.27.
UCSCiY56A3A.27.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057032 mRNA. Translation: AAC13567.1 .
AL132860 Genomic DNA. Translation: CAB60518.2 .
PIRi T43031.
RefSeqi NP_499558.1. NM_067157.4.
UniGenei Cel.19512.

3D structure databases

ProteinModelPortali O61660.
SMRi O61660. Positions 1-608.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 6239.Y56A3A.27.2.

Proteomic databases

PaxDbi O61660.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai Y56A3A.27 ; Y56A3A.27 ; WBGene00006596 .
GeneIDi 176631.
KEGGi cel:CELE_Y56A3A.27.
UCSCi Y56A3A.27.1. c. elegans.

Organism-specific databases

CTDi 176631.
WormBasei Y56A3A.27 ; CE28138 ; WBGene00006596 ; top-3.

Phylogenomic databases

eggNOGi COG0550.
GeneTreei ENSGT00530000063430.
HOGENOMi HOG000184377.
InParanoidi O61660.
KOi K03165.
OMAi VVILYER.
PhylomeDBi O61660.

Miscellaneous databases

NextBioi 893376.
PROi O61660.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR006171. Toprim_domain.
IPR010666. Znf_GRF.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF06839. zf-GRF. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional characterization of Caenorhabditis elegans DNA topoisomerase IIIalpha."
    Kim Y.-C., Lee J., Koo H.-S.
    Nucleic Acids Res. 28:2012-2017(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiTOP3_CAEEL
AccessioniPrimary (citable) accession number: O61660
Secondary accession number(s): Q9U223
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3