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O61660

- TOP3_CAEEL

UniProt

O61660 - TOP3_CAEEL

Protein

DNA topoisomerase 3

Gene

top-3

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei334 – 3341O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-EC
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. DNA topological change Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 3 (EC:5.99.1.2)
    Alternative name(s):
    DNA topoisomerase III
    Gene namesi
    Name:top-3
    ORF Names:Y56A3A.27
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiY56A3A.27; CE28138; WBGene00006596; top-3.

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 759759DNA topoisomerase 3PRO_0000145194Add
    BLAST

    Proteomic databases

    PaxDbiO61660.

    Interactioni

    Protein-protein interaction databases

    STRINGi6239.Y56A3A.27.2.

    Structurei

    3D structure databases

    ProteinModelPortaliO61660.
    SMRiO61660. Positions 1-608.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 147145ToprimPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.Curated
    Contains 1 Toprim domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0550.
    GeneTreeiENSGT00530000063430.
    HOGENOMiHOG000184377.
    InParanoidiO61660.
    KOiK03165.
    OMAiVVILYER.
    PhylomeDBiO61660.

    Family and domain databases

    Gene3Di1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR006171. Toprim_domain.
    IPR010666. Znf_GRF.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF06839. zf-GRF. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O61660-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRALFVAEK NDVAKGVAAI LSNGTANRRE GRSKFNKIYT LNTELFGQQT    50
    AISVTSVSGH MMNFQFHENM SNWQTASMVE LFRAPVRHVV TPEMKLIEQT 100
    LREQAQRHDI LVVWTDCDRE GEAIGAEIVK VCRDSNRRLD IFRARFSEIT 150
    KAAITRAARN LIRLDEKTVA AVDCRSELDL RIGSAFTRLQ TLHLRNRFRD 200
    LLGQNDTSQV ISYGSCQFPT LGFVTDRYKM IENFVSEPFW KLIVEHTRES 250
    HKVEFLWDRN RLFDRDTVDI LHDECKETKE AHVEKVAKKP KSKWRPQALD 300
    TVELEKLGIS KLRMSAKQTM QVAEKLYSKG FISYPRTETN KFPAGLNLTP 350
    LVQQQTQSNI WGDFANEVLQ NGVNPRNGRK SDEAHPPIHP LKFTEKHQLQ 400
    GDDWKVYELV VRHFLACVSQ DAQGEETMVN LTVGTEKFHA SGLRIRDMGY 450
    LKVYVYEKWG NRLLPTYTEG ERFTDFELKI GDGKTQAPDF LTEADLISLM 500
    DKYGIGTDAT HAEHIEKIKT REYIGVRPDG KLIPSFLGLA LVDGYDDMGF 550
    AMSKPDLRAN LEIGLKEICD GRRQKQEVLD EQIGKYRAIF VESERKIGVL 600
    SQSLQRYLDK NNQAGGGPGG PGGGGGPPRG PGGGGGGGPT GPPAPPKPPA 650
    KPRGRPPRKS ISPAVKNGHD DPENDTIVTL SEVFGSMSNP KPARKPRAPR 700
    KSAAPKEQEE EEEVFCQCPE PMRAVTKVVQ KEGPNKGKKF YTCSLPYTSS 750
    EKCNFFKWA 759
    Length:759
    Mass (Da):85,438
    Last modified:August 1, 1998 - v1
    Checksum:i3D862412D72946BD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF057032 mRNA. Translation: AAC13567.1.
    AL132860 Genomic DNA. Translation: CAB60518.2.
    PIRiT43031.
    RefSeqiNP_499558.1. NM_067157.4.
    UniGeneiCel.19512.

    Genome annotation databases

    EnsemblMetazoaiY56A3A.27; Y56A3A.27; WBGene00006596.
    GeneIDi176631.
    KEGGicel:CELE_Y56A3A.27.
    UCSCiY56A3A.27.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF057032 mRNA. Translation: AAC13567.1 .
    AL132860 Genomic DNA. Translation: CAB60518.2 .
    PIRi T43031.
    RefSeqi NP_499558.1. NM_067157.4.
    UniGenei Cel.19512.

    3D structure databases

    ProteinModelPortali O61660.
    SMRi O61660. Positions 1-608.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6239.Y56A3A.27.2.

    Proteomic databases

    PaxDbi O61660.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai Y56A3A.27 ; Y56A3A.27 ; WBGene00006596 .
    GeneIDi 176631.
    KEGGi cel:CELE_Y56A3A.27.
    UCSCi Y56A3A.27.1. c. elegans.

    Organism-specific databases

    CTDi 176631.
    WormBasei Y56A3A.27 ; CE28138 ; WBGene00006596 ; top-3.

    Phylogenomic databases

    eggNOGi COG0550.
    GeneTreei ENSGT00530000063430.
    HOGENOMi HOG000184377.
    InParanoidi O61660.
    KOi K03165.
    OMAi VVILYER.
    PhylomeDBi O61660.

    Miscellaneous databases

    NextBioi 893376.
    PROi O61660.

    Family and domain databases

    Gene3Di 1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR006171. Toprim_domain.
    IPR010666. Znf_GRF.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF06839. zf-GRF. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional characterization of Caenorhabditis elegans DNA topoisomerase IIIalpha."
      Kim Y.-C., Lee J., Koo H.-S.
      Nucleic Acids Res. 28:2012-2017(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Bristol N2.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiTOP3_CAEEL
    AccessioniPrimary (citable) accession number: O61660
    Secondary accession number(s): Q9U223
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3