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O61608

- NOS_ANOST

UniProt

O61608 - NOS_ANOST

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Protein
Nitric oxide synthase
Gene
N/A
Organism
Anopheles stephensi (Indo-Pakistan malaria mosquito)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide limits plasmodium development in the midgut.1 Publication

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Heme group By similarity.
Binds 1 FAD By similarity.
Binds 1 FMN By similarity.

Enzyme regulationi

Stimulated by calcium/calmodulin By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi224 – 2241Iron (heme axial ligand) By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi712 – 74332FMN By similarity
Add
BLAST
Nucleotide bindingi855 – 86612FAD By similarity
Add
BLAST
Nucleotide bindingi998 – 100811FAD By similarity
Add
BLAST
Nucleotide bindingi1073 – 109119NADP By similarity
Add
BLAST
Nucleotide bindingi1170 – 118516NADP By similarity
Add
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. NADP binding Source: InterPro
  3. flavin adenine dinucleotide binding Source: InterPro
  4. heme binding Source: InterPro
  5. iron ion binding Source: InterPro
  6. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase (EC:1.14.13.39)
Short name:
NOS
OrganismiAnopheles stephensi (Indo-Pakistan malaria mosquito)
Taxonomic identifieri30069 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12471247Nitric oxide synthase
PRO_0000170950Add
BLAST

Expressioni

Inductioni

Detected in the midgut and carcass soon after invasion of the midgut by plasmodium. Early induction is also primed by bacterial growth in the blood meal.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO61608.
SMRiO61608. Positions 113-525.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini567 – 766200Flavodoxin-like
Add
BLAST
Domaini795 – 1065271FAD-binding FR-type
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni537 – 55721Calmodulin-binding Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi51 – 544Poly-Gly

Sequence similaritiesi

Belongs to the NOS family.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O61608-1 [UniParc]FASTAAdd to Basket

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MADTTTVVVE RREVAEGRES SKANHIGEER RGYDVSRKRC SISVHGGGTE     50
GGGGNMRTNY RELSPASLRI HRKSSHDIRN TLLGPDGEVL HLHDPSGKGG 100
DGMGKMPAVV KPIKLKSIVT KAESYDTMHG KASDVMSCSR EVCMGSVMTP 150
HVIGTETRKP EIVQQHAKDF LDQYYSSIRR LKSPAHDSRW QQVQKEVEAT 200
GSYHLTETEL IYGAKLAWRN SSRCIGRIQW SKLQVFDCRY VTTTSGMFEA 250
ICNHIKYATN KGNLRSAITI FPQRTDGKHD YRIWNNQIIS YAGYKNADGK 300
IIGDPANVEF TDFCVKLGWK SKRTEWDILP LVVSANGHDP DYFDYPPELI 350
LEVPLSHPQF KWFAELNLRW YAVPMVSSML FDCGGIQFTA TAFSGWYMST 400
EIGCRNLCDA NRRNLLEPIA IKMGLDTRNP TSLWKDKALV EINIAVLHSY 450
QSRNITIVDH HTASESFMKH FENETKLRNG CPADWIWIVP PMSASVTPVF 500
HQEMAVYYLR PSFEYQESAM KTHIWKKGRD SAKNKKPRRK FNFKQIARAV 550
KFTSKLFGRA LSRRIKATVL YATETGRSEQ YARQLVELLG HAFNAQIYCM 600
SDYDISSIEH EALLLVVAST FGNGDPPENG ELFAQDLYAM KLHESGHHQA 650
HSELTIAASS KSFIKANSRS DLGKFGPMGG RKIDRLDSLR GSTTDTLSEE 700
TFGPLSNVRF AVFALGSSAY PNFCAFGKYI DNILGELGGE RLMKMATGDE 750
ICGQEQAFRK WAPEVFKIAC ETFCLDPEET LSDAAFALQS ELSENTVRYA 800
PVAEYESLDR ALSKFHNKKS MECSVKRNPI NLHCEMNGTE RSTILVEIMA 850
EGIDYEPGDH VGIFPANRKE IVDGIIERLT GVNDPDEMLQ LQVLKEKQTQ 900
NGVYKSWEPH ERLPVCTLRT LLTRFLDITT PPTRQLLTYL ASCCGDKADE 950
ERLLMLANES SVYEDWRYWK LPHLLEVLEE FPSCRPPAAV FVAQLNALQP 1000
RFYSISSSPR KYSNEIHLTV AIVTYRAEDG EGAEHYGVCS NYLANLQSDD 1050
KIYLFVRSAP SFHMSKDRTK PVILIGPGTG IAPFRSFWQE WDHIKTEMVD 1100
CKIPKVWLFF GCRTKNVDLY RDEKEEMVQH GVLDRVFLAL SREENIPKTY 1150
VQDLALKEAE SISELIMQEK GHIYVCGDVT MAEHVYQTLR KILATREKRT 1200
ETEMEKYMLT LRDENRYHED IFGITLRTAE IHNKSRATAR IRMASQP 1247
Length:1,247
Mass (Da):141,651
Last modified:January 1, 1999 - v2
Checksum:i5F435E5EAC3D3778
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF130134
, AF130124, AF130125, AF130126, AF130127, AF130128, AF130129, AF130130, AF130131, AF130132, AF130133 Genomic DNA. Translation: AAC68577.1.
PIRiT31331.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF130134
, AF130124 , AF130125 , AF130126 , AF130127 , AF130128 , AF130129 , AF130130 , AF130131 , AF130132 , AF130133 Genomic DNA. Translation: AAC68577.1 .
PIRi T31331.

3D structure databases

ProteinModelPortali O61608.
SMRi O61608. Positions 113-525.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000333. NOS. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The mosquito Anopheles stephensi limits malaria parasite development with inducible synthesis of nitric oxide."
    Luckhart S., Vodovotz Y., Cui L., Rosenberg R.
    Proc. Natl. Acad. Sci. U.S.A. 95:5700-5705(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
  2. "Gene structure and polymorphism of an invertebrate nitric oxide synthase gene."
    Luckhart S., Rosenberg R.
    Gene 232:25-34(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiNOS_ANOST
AccessioniPrimary (citable) accession number: O61608
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1999
Last modified: September 3, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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