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O61608

- NOS_ANOST

UniProt

O61608 - NOS_ANOST

Protein

Nitric oxide synthase

Gene
N/A
Organism
Anopheles stephensi (Indo-Pakistan malaria mosquito)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (01 Jan 1999)
      Previous versions | rss
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    Functioni

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide limits plasmodium development in the midgut.1 Publication

    Catalytic activityi

    2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

    Cofactori

    Heme group.By similarity
    Binds 1 FAD.By similarity
    Binds 1 FMN.By similarity

    Enzyme regulationi

    Stimulated by calcium/calmodulin.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi224 – 2241Iron (heme axial ligand)By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi712 – 74332FMNPROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi855 – 86612FADBy similarityAdd
    BLAST
    Nucleotide bindingi998 – 100811FADBy similarityAdd
    BLAST
    Nucleotide bindingi1073 – 109119NADPBy similarityAdd
    BLAST
    Nucleotide bindingi1170 – 118516NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. FMN binding Source: InterPro
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro
    5. NADP binding Source: InterPro
    6. nitric-oxide synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitric oxide biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitric oxide synthase (EC:1.14.13.39)
    Short name:
    NOS
    OrganismiAnopheles stephensi (Indo-Pakistan malaria mosquito)
    Taxonomic identifieri30069 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12471247Nitric oxide synthasePRO_0000170950Add
    BLAST

    Expressioni

    Inductioni

    Detected in the midgut and carcass soon after invasion of the midgut by plasmodium. Early induction is also primed by bacterial growth in the blood meal.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO61608.
    SMRiO61608. Positions 113-525.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini567 – 766200Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini795 – 1065271FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni537 – 55721Calmodulin-bindingSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi51 – 544Poly-Gly

    Sequence similaritiesi

    Belongs to the NOS family.Curated
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 2 hits.
    3.90.340.10. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000333. NOS. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O61608-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADTTTVVVE RREVAEGRES SKANHIGEER RGYDVSRKRC SISVHGGGTE     50
    GGGGNMRTNY RELSPASLRI HRKSSHDIRN TLLGPDGEVL HLHDPSGKGG 100
    DGMGKMPAVV KPIKLKSIVT KAESYDTMHG KASDVMSCSR EVCMGSVMTP 150
    HVIGTETRKP EIVQQHAKDF LDQYYSSIRR LKSPAHDSRW QQVQKEVEAT 200
    GSYHLTETEL IYGAKLAWRN SSRCIGRIQW SKLQVFDCRY VTTTSGMFEA 250
    ICNHIKYATN KGNLRSAITI FPQRTDGKHD YRIWNNQIIS YAGYKNADGK 300
    IIGDPANVEF TDFCVKLGWK SKRTEWDILP LVVSANGHDP DYFDYPPELI 350
    LEVPLSHPQF KWFAELNLRW YAVPMVSSML FDCGGIQFTA TAFSGWYMST 400
    EIGCRNLCDA NRRNLLEPIA IKMGLDTRNP TSLWKDKALV EINIAVLHSY 450
    QSRNITIVDH HTASESFMKH FENETKLRNG CPADWIWIVP PMSASVTPVF 500
    HQEMAVYYLR PSFEYQESAM KTHIWKKGRD SAKNKKPRRK FNFKQIARAV 550
    KFTSKLFGRA LSRRIKATVL YATETGRSEQ YARQLVELLG HAFNAQIYCM 600
    SDYDISSIEH EALLLVVAST FGNGDPPENG ELFAQDLYAM KLHESGHHQA 650
    HSELTIAASS KSFIKANSRS DLGKFGPMGG RKIDRLDSLR GSTTDTLSEE 700
    TFGPLSNVRF AVFALGSSAY PNFCAFGKYI DNILGELGGE RLMKMATGDE 750
    ICGQEQAFRK WAPEVFKIAC ETFCLDPEET LSDAAFALQS ELSENTVRYA 800
    PVAEYESLDR ALSKFHNKKS MECSVKRNPI NLHCEMNGTE RSTILVEIMA 850
    EGIDYEPGDH VGIFPANRKE IVDGIIERLT GVNDPDEMLQ LQVLKEKQTQ 900
    NGVYKSWEPH ERLPVCTLRT LLTRFLDITT PPTRQLLTYL ASCCGDKADE 950
    ERLLMLANES SVYEDWRYWK LPHLLEVLEE FPSCRPPAAV FVAQLNALQP 1000
    RFYSISSSPR KYSNEIHLTV AIVTYRAEDG EGAEHYGVCS NYLANLQSDD 1050
    KIYLFVRSAP SFHMSKDRTK PVILIGPGTG IAPFRSFWQE WDHIKTEMVD 1100
    CKIPKVWLFF GCRTKNVDLY RDEKEEMVQH GVLDRVFLAL SREENIPKTY 1150
    VQDLALKEAE SISELIMQEK GHIYVCGDVT MAEHVYQTLR KILATREKRT 1200
    ETEMEKYMLT LRDENRYHED IFGITLRTAE IHNKSRATAR IRMASQP 1247
    Length:1,247
    Mass (Da):141,651
    Last modified:January 1, 1999 - v2
    Checksum:i5F435E5EAC3D3778
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF130134
    , AF130124, AF130125, AF130126, AF130127, AF130128, AF130129, AF130130, AF130131, AF130132, AF130133 Genomic DNA. Translation: AAC68577.1.
    PIRiT31331.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF130134
    , AF130124 , AF130125 , AF130126 , AF130127 , AF130128 , AF130129 , AF130130 , AF130131 , AF130132 , AF130133 Genomic DNA. Translation: AAC68577.1 .
    PIRi T31331.

    3D structure databases

    ProteinModelPortali O61608.
    SMRi O61608. Positions 113-525.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 2 hits.
    3.90.340.10. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000333. NOS. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The mosquito Anopheles stephensi limits malaria parasite development with inducible synthesis of nitric oxide."
      Luckhart S., Vodovotz Y., Cui L., Rosenberg R.
      Proc. Natl. Acad. Sci. U.S.A. 95:5700-5705(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
    2. "Gene structure and polymorphism of an invertebrate nitric oxide synthase gene."
      Luckhart S., Rosenberg R.
      Gene 232:25-34(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiNOS_ANOST
    AccessioniPrimary (citable) accession number: O61608
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 1, 1999
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3