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O61608 (NOS_ANOST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nitric oxide synthase

Short name=NOS
EC=1.14.13.39
OrganismAnopheles stephensi (Indo-Pakistan malaria mosquito)
Taxonomic identifier30069 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles

Protein attributes

Sequence length1247 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide limits plasmodium development in the midgut. Ref.1

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Enzyme regulation

Stimulated by calcium/calmodulin By similarity.

Induction

Detected in the midgut and carcass soon after invasion of the midgut by plasmodium. Early induction is also primed by bacterial growth in the blood meal. Ref.1

Sequence similarities

Belongs to the NOS family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12471247Nitric oxide synthase
PRO_0000170950

Regions

Domain567 – 766200Flavodoxin-like
Domain795 – 1065271FAD-binding FR-type
Nucleotide binding712 – 74332FMN By similarity
Nucleotide binding855 – 86612FAD By similarity
Nucleotide binding998 – 100811FAD By similarity
Nucleotide binding1073 – 109119NADP By similarity
Nucleotide binding1170 – 118516NADP By similarity
Region537 – 55721Calmodulin-binding Potential
Compositional bias51 – 544Poly-Gly

Sites

Metal binding2241Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
O61608 [UniParc].

Last modified January 1, 1999. Version 2.
Checksum: 5F435E5EAC3D3778

FASTA1,247141,651
        10         20         30         40         50         60 
MADTTTVVVE RREVAEGRES SKANHIGEER RGYDVSRKRC SISVHGGGTE GGGGNMRTNY 

        70         80         90        100        110        120 
RELSPASLRI HRKSSHDIRN TLLGPDGEVL HLHDPSGKGG DGMGKMPAVV KPIKLKSIVT 

       130        140        150        160        170        180 
KAESYDTMHG KASDVMSCSR EVCMGSVMTP HVIGTETRKP EIVQQHAKDF LDQYYSSIRR 

       190        200        210        220        230        240 
LKSPAHDSRW QQVQKEVEAT GSYHLTETEL IYGAKLAWRN SSRCIGRIQW SKLQVFDCRY 

       250        260        270        280        290        300 
VTTTSGMFEA ICNHIKYATN KGNLRSAITI FPQRTDGKHD YRIWNNQIIS YAGYKNADGK 

       310        320        330        340        350        360 
IIGDPANVEF TDFCVKLGWK SKRTEWDILP LVVSANGHDP DYFDYPPELI LEVPLSHPQF 

       370        380        390        400        410        420 
KWFAELNLRW YAVPMVSSML FDCGGIQFTA TAFSGWYMST EIGCRNLCDA NRRNLLEPIA 

       430        440        450        460        470        480 
IKMGLDTRNP TSLWKDKALV EINIAVLHSY QSRNITIVDH HTASESFMKH FENETKLRNG 

       490        500        510        520        530        540 
CPADWIWIVP PMSASVTPVF HQEMAVYYLR PSFEYQESAM KTHIWKKGRD SAKNKKPRRK 

       550        560        570        580        590        600 
FNFKQIARAV KFTSKLFGRA LSRRIKATVL YATETGRSEQ YARQLVELLG HAFNAQIYCM 

       610        620        630        640        650        660 
SDYDISSIEH EALLLVVAST FGNGDPPENG ELFAQDLYAM KLHESGHHQA HSELTIAASS 

       670        680        690        700        710        720 
KSFIKANSRS DLGKFGPMGG RKIDRLDSLR GSTTDTLSEE TFGPLSNVRF AVFALGSSAY 

       730        740        750        760        770        780 
PNFCAFGKYI DNILGELGGE RLMKMATGDE ICGQEQAFRK WAPEVFKIAC ETFCLDPEET 

       790        800        810        820        830        840 
LSDAAFALQS ELSENTVRYA PVAEYESLDR ALSKFHNKKS MECSVKRNPI NLHCEMNGTE 

       850        860        870        880        890        900 
RSTILVEIMA EGIDYEPGDH VGIFPANRKE IVDGIIERLT GVNDPDEMLQ LQVLKEKQTQ 

       910        920        930        940        950        960 
NGVYKSWEPH ERLPVCTLRT LLTRFLDITT PPTRQLLTYL ASCCGDKADE ERLLMLANES 

       970        980        990       1000       1010       1020 
SVYEDWRYWK LPHLLEVLEE FPSCRPPAAV FVAQLNALQP RFYSISSSPR KYSNEIHLTV 

      1030       1040       1050       1060       1070       1080 
AIVTYRAEDG EGAEHYGVCS NYLANLQSDD KIYLFVRSAP SFHMSKDRTK PVILIGPGTG 

      1090       1100       1110       1120       1130       1140 
IAPFRSFWQE WDHIKTEMVD CKIPKVWLFF GCRTKNVDLY RDEKEEMVQH GVLDRVFLAL 

      1150       1160       1170       1180       1190       1200 
SREENIPKTY VQDLALKEAE SISELIMQEK GHIYVCGDVT MAEHVYQTLR KILATREKRT 

      1210       1220       1230       1240 
ETEMEKYMLT LRDENRYHED IFGITLRTAE IHNKSRATAR IRMASQP 

« Hide

References

[1]"The mosquito Anopheles stephensi limits malaria parasite development with inducible synthesis of nitric oxide."
Luckhart S., Vodovotz Y., Cui L., Rosenberg R.
Proc. Natl. Acad. Sci. U.S.A. 95:5700-5705(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
[2]"Gene structure and polymorphism of an invertebrate nitric oxide synthase gene."
Luckhart S., Rosenberg R.
Gene 232:25-34(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF130134 expand/collapse EMBL AC list , AF130124, AF130125, AF130126, AF130127, AF130128, AF130129, AF130130, AF130131, AF130132, AF130133 Genomic DNA. Translation: AAC68577.1.
PIRT31331.

3D structure databases

ProteinModelPortalO61608.
SMRO61608. Positions 113-525.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR004030. NO_synthase_oxygenase_dom.
IPR012144. NOS_euk.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF000333. NOS. 1 hit.
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNOS_ANOST
AccessionPrimary (citable) accession number: O61608
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1999
Last modified: June 11, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families