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O61608

- NOS_ANOST

UniProt

O61608 - NOS_ANOST

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Protein

Nitric oxide synthase

Gene
N/A
Organism
Anopheles stephensi (Indo-Pakistan malaria mosquito)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide limits plasmodium development in the midgut.1 Publication

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarity
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • FMNBy similarityNote: Binds 1 FMN.By similarity

Enzyme regulationi

Stimulated by calcium/calmodulin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi224 – 2241Iron (heme axial ligand)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi712 – 74332FMNPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi855 – 86612FADBy similarityAdd
BLAST
Nucleotide bindingi998 – 100811FADBy similarityAdd
BLAST
Nucleotide bindingi1073 – 109119NADPBy similarityAdd
BLAST
Nucleotide bindingi1170 – 118516NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. FMN binding Source: InterPro
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. NADP binding Source: InterPro
  6. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase (EC:1.14.13.39)
Short name:
NOS
OrganismiAnopheles stephensi (Indo-Pakistan malaria mosquito)
Taxonomic identifieri30069 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12471247Nitric oxide synthasePRO_0000170950Add
BLAST

Expressioni

Inductioni

Detected in the midgut and carcass soon after invasion of the midgut by plasmodium. Early induction is also primed by bacterial growth in the blood meal.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO61608.
SMRiO61608. Positions 113-525.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini567 – 766200Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini795 – 1065271FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni537 – 55721Calmodulin-bindingSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi51 – 544Poly-Gly

Sequence similaritiesi

Belongs to the NOS family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O61608-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADTTTVVVE RREVAEGRES SKANHIGEER RGYDVSRKRC SISVHGGGTE
60 70 80 90 100
GGGGNMRTNY RELSPASLRI HRKSSHDIRN TLLGPDGEVL HLHDPSGKGG
110 120 130 140 150
DGMGKMPAVV KPIKLKSIVT KAESYDTMHG KASDVMSCSR EVCMGSVMTP
160 170 180 190 200
HVIGTETRKP EIVQQHAKDF LDQYYSSIRR LKSPAHDSRW QQVQKEVEAT
210 220 230 240 250
GSYHLTETEL IYGAKLAWRN SSRCIGRIQW SKLQVFDCRY VTTTSGMFEA
260 270 280 290 300
ICNHIKYATN KGNLRSAITI FPQRTDGKHD YRIWNNQIIS YAGYKNADGK
310 320 330 340 350
IIGDPANVEF TDFCVKLGWK SKRTEWDILP LVVSANGHDP DYFDYPPELI
360 370 380 390 400
LEVPLSHPQF KWFAELNLRW YAVPMVSSML FDCGGIQFTA TAFSGWYMST
410 420 430 440 450
EIGCRNLCDA NRRNLLEPIA IKMGLDTRNP TSLWKDKALV EINIAVLHSY
460 470 480 490 500
QSRNITIVDH HTASESFMKH FENETKLRNG CPADWIWIVP PMSASVTPVF
510 520 530 540 550
HQEMAVYYLR PSFEYQESAM KTHIWKKGRD SAKNKKPRRK FNFKQIARAV
560 570 580 590 600
KFTSKLFGRA LSRRIKATVL YATETGRSEQ YARQLVELLG HAFNAQIYCM
610 620 630 640 650
SDYDISSIEH EALLLVVAST FGNGDPPENG ELFAQDLYAM KLHESGHHQA
660 670 680 690 700
HSELTIAASS KSFIKANSRS DLGKFGPMGG RKIDRLDSLR GSTTDTLSEE
710 720 730 740 750
TFGPLSNVRF AVFALGSSAY PNFCAFGKYI DNILGELGGE RLMKMATGDE
760 770 780 790 800
ICGQEQAFRK WAPEVFKIAC ETFCLDPEET LSDAAFALQS ELSENTVRYA
810 820 830 840 850
PVAEYESLDR ALSKFHNKKS MECSVKRNPI NLHCEMNGTE RSTILVEIMA
860 870 880 890 900
EGIDYEPGDH VGIFPANRKE IVDGIIERLT GVNDPDEMLQ LQVLKEKQTQ
910 920 930 940 950
NGVYKSWEPH ERLPVCTLRT LLTRFLDITT PPTRQLLTYL ASCCGDKADE
960 970 980 990 1000
ERLLMLANES SVYEDWRYWK LPHLLEVLEE FPSCRPPAAV FVAQLNALQP
1010 1020 1030 1040 1050
RFYSISSSPR KYSNEIHLTV AIVTYRAEDG EGAEHYGVCS NYLANLQSDD
1060 1070 1080 1090 1100
KIYLFVRSAP SFHMSKDRTK PVILIGPGTG IAPFRSFWQE WDHIKTEMVD
1110 1120 1130 1140 1150
CKIPKVWLFF GCRTKNVDLY RDEKEEMVQH GVLDRVFLAL SREENIPKTY
1160 1170 1180 1190 1200
VQDLALKEAE SISELIMQEK GHIYVCGDVT MAEHVYQTLR KILATREKRT
1210 1220 1230 1240
ETEMEKYMLT LRDENRYHED IFGITLRTAE IHNKSRATAR IRMASQP
Length:1,247
Mass (Da):141,651
Last modified:January 1, 1999 - v2
Checksum:i5F435E5EAC3D3778
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF130134
, AF130124, AF130125, AF130126, AF130127, AF130128, AF130129, AF130130, AF130131, AF130132, AF130133 Genomic DNA. Translation: AAC68577.1.
PIRiT31331.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF130134
, AF130124 , AF130125 , AF130126 , AF130127 , AF130128 , AF130129 , AF130130 , AF130131 , AF130132 , AF130133 Genomic DNA. Translation: AAC68577.1 .
PIRi T31331.

3D structure databases

ProteinModelPortali O61608.
SMRi O61608. Positions 113-525.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000333. NOS. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The mosquito Anopheles stephensi limits malaria parasite development with inducible synthesis of nitric oxide."
    Luckhart S., Vodovotz Y., Cui L., Rosenberg R.
    Proc. Natl. Acad. Sci. U.S.A. 95:5700-5705(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
  2. "Gene structure and polymorphism of an invertebrate nitric oxide synthase gene."
    Luckhart S., Rosenberg R.
    Gene 232:25-34(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiNOS_ANOST
AccessioniPrimary (citable) accession number: O61608
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1999
Last modified: November 26, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3