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Protein

Nitric oxide synthase

Gene
N/A
Organism
Anopheles stephensi (Indo-Pakistan malaria mosquito)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide limits plasmodium development in the midgut.1 Publication

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarity
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • FMNBy similarityNote: Binds 1 FMN.By similarity

Enzyme regulationi

Stimulated by calcium/calmodulin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi224Iron (heme axial ligand)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi712 – 743FMNAdd BLAST32
Nucleotide bindingi855 – 866FADBy similarityAdd BLAST12
Nucleotide bindingi998 – 1008FADBy similarityAdd BLAST11
Nucleotide bindingi1073 – 1091NADPBy similarityAdd BLAST19
Nucleotide bindingi1170 – 1185NADPBy similarityAdd BLAST16

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Oxidoreductase
LigandFAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase (EC:1.14.13.39)
Short name:
NOS
OrganismiAnopheles stephensi (Indo-Pakistan malaria mosquito)
Taxonomic identifieri30069 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
Proteomesi
  • UP000078201 Componenti: Unassembled WGS sequence
  • UP000076408 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001709501 – 1247Nitric oxide synthaseAdd BLAST1247

Expressioni

Inductioni

Detected in the midgut and carcass soon after invasion of the midgut by plasmodium. Early induction is also primed by bacterial growth in the blood meal.1 Publication

Interactioni

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliO61608.
SMRiO61608.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini567 – 766Flavodoxin-likeAdd BLAST200
Domaini795 – 1065FAD-binding FR-typeAdd BLAST271

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni537 – 557Calmodulin-bindingSequence analysisAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi51 – 54Poly-Gly4

Sequence similaritiesi

Belongs to the NOS family.Curated

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
InterProiView protein in InterPro
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_sf.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR036119. NOS_N_sf.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
PfamiView protein in Pfam
PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiView protein in PROSITE
PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.

Sequencei

Sequence statusi: Complete.

O61608-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADTTTVVVE RREVAEGRES SKANHIGEER RGYDVSRKRC SISVHGGGTE
60 70 80 90 100
GGGGNMRTNY RELSPASLRI HRKSSHDIRN TLLGPDGEVL HLHDPSGKGG
110 120 130 140 150
DGMGKMPAVV KPIKLKSIVT KAESYDTMHG KASDVMSCSR EVCMGSVMTP
160 170 180 190 200
HVIGTETRKP EIVQQHAKDF LDQYYSSIRR LKSPAHDSRW QQVQKEVEAT
210 220 230 240 250
GSYHLTETEL IYGAKLAWRN SSRCIGRIQW SKLQVFDCRY VTTTSGMFEA
260 270 280 290 300
ICNHIKYATN KGNLRSAITI FPQRTDGKHD YRIWNNQIIS YAGYKNADGK
310 320 330 340 350
IIGDPANVEF TDFCVKLGWK SKRTEWDILP LVVSANGHDP DYFDYPPELI
360 370 380 390 400
LEVPLSHPQF KWFAELNLRW YAVPMVSSML FDCGGIQFTA TAFSGWYMST
410 420 430 440 450
EIGCRNLCDA NRRNLLEPIA IKMGLDTRNP TSLWKDKALV EINIAVLHSY
460 470 480 490 500
QSRNITIVDH HTASESFMKH FENETKLRNG CPADWIWIVP PMSASVTPVF
510 520 530 540 550
HQEMAVYYLR PSFEYQESAM KTHIWKKGRD SAKNKKPRRK FNFKQIARAV
560 570 580 590 600
KFTSKLFGRA LSRRIKATVL YATETGRSEQ YARQLVELLG HAFNAQIYCM
610 620 630 640 650
SDYDISSIEH EALLLVVAST FGNGDPPENG ELFAQDLYAM KLHESGHHQA
660 670 680 690 700
HSELTIAASS KSFIKANSRS DLGKFGPMGG RKIDRLDSLR GSTTDTLSEE
710 720 730 740 750
TFGPLSNVRF AVFALGSSAY PNFCAFGKYI DNILGELGGE RLMKMATGDE
760 770 780 790 800
ICGQEQAFRK WAPEVFKIAC ETFCLDPEET LSDAAFALQS ELSENTVRYA
810 820 830 840 850
PVAEYESLDR ALSKFHNKKS MECSVKRNPI NLHCEMNGTE RSTILVEIMA
860 870 880 890 900
EGIDYEPGDH VGIFPANRKE IVDGIIERLT GVNDPDEMLQ LQVLKEKQTQ
910 920 930 940 950
NGVYKSWEPH ERLPVCTLRT LLTRFLDITT PPTRQLLTYL ASCCGDKADE
960 970 980 990 1000
ERLLMLANES SVYEDWRYWK LPHLLEVLEE FPSCRPPAAV FVAQLNALQP
1010 1020 1030 1040 1050
RFYSISSSPR KYSNEIHLTV AIVTYRAEDG EGAEHYGVCS NYLANLQSDD
1060 1070 1080 1090 1100
KIYLFVRSAP SFHMSKDRTK PVILIGPGTG IAPFRSFWQE WDHIKTEMVD
1110 1120 1130 1140 1150
CKIPKVWLFF GCRTKNVDLY RDEKEEMVQH GVLDRVFLAL SREENIPKTY
1160 1170 1180 1190 1200
VQDLALKEAE SISELIMQEK GHIYVCGDVT MAEHVYQTLR KILATREKRT
1210 1220 1230 1240
ETEMEKYMLT LRDENRYHED IFGITLRTAE IHNKSRATAR IRMASQP
Length:1,247
Mass (Da):141,651
Last modified:January 1, 1999 - v2
Checksum:i5F435E5EAC3D3778
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF130134
, AF130124, AF130125, AF130126, AF130127, AF130128, AF130129, AF130130, AF130131, AF130132, AF130133 Genomic DNA. Translation: AAC68577.1.
PIRiT31331.

Genome annotation databases

VectorBaseiASTEI03015-RA; ASTEI03015-PA; ASTEI03015.

Similar proteinsi

Entry informationi

Entry nameiNOS_ANOST
AccessioniPrimary (citable) accession number: O61608
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1999
Last modified: November 22, 2017
This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families