Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O61460 (VAB1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ephrin receptor 1

EC=2.7.10.1
Alternative name(s):
Tyrosine-protein kinase Eph receptor
Variable abnormal protein 1
Gene names
Name:vab-1
ORF Names:M03A1.1
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length1122 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for members of the ephrin family. Involved in interactions between neuronal substrate cells and a migrating epithelial sheet in head epidermis morphogenesis. Also required for cell movements following gastrulation and during ventral closure of the epidermis. Ref.1 UniProtKB Q62413

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

During ventral enclosure of the epidermis, expression is seen in clusters of presumptive head neuronal cells and several cells in the tail region. Early larvae show expression in the nerve ring and ventral nerve cord. Strong expression is also seen in the procorpus and terminal bulb of the pharynx. Expression in the nervous system is seen through to adulthood. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Contains 1 Eph LBD (Eph ligand-binding) domain.

Contains 2 fibronectin type-III domains.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainRepeat
Signal
Transmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   Molecular functionDevelopmental protein
Kinase
Receptor
Transferase
Tyrosine-protein kinase
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaxon guidance

Inferred from mutant phenotype PubMed 10409513. Source: WormBase

axonogenesis

Inferred from mutant phenotype PubMed 10409513. Source: WormBase

embryo development

Inferred from mutant phenotype Ref.1. Source: WormBase

embryonic body morphogenesis

Inferred from mutant phenotype Ref.1. Source: UniProtKB

ephrin receptor signaling pathway

Inferred from electronic annotation. Source: InterPro

morphogenesis of embryonic epithelium

Inferred from mutant phenotype Ref.1. Source: UniProtKB

oocyte maturation

Inferred from mutant phenotype PubMed 12533508. Source: WormBase

peptidyl-tyrosine phosphorylation

Inferred from direct assay PubMed 19853560. Source: WormBase

signal transduction by phosphorylation

Inferred from sequence or structural similarity Ref.1. Source: GOC

   Cellular_componentaxon

Inferred from direct assay Ref.1. Source: WormBase

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from direct assay PubMed 16267094. Source: WormBase

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein domain specific binding

Inferred from physical interaction PubMed 18555774. Source: WormBase

protein tyrosine kinase activity

Inferred from direct assay PubMed 19853560. Source: WormBase

receptor signaling protein tyrosine kinase activity

Inferred from sequence or structural similarity Ref.1. Source: WormBase

transmembrane receptor protein tyrosine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

daf-18G5EE013EBI-1788319,EBI-2914422
vpr-1O447822EBI-1788319,EBI-320991

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: O61460-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: O61460-2)

The sequence of this isoform differs from the canonical sequence as follows:
     843-847: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 11221093Ephrin receptor 1
PRO_0000016839

Regions

Topological domain30 – 559530Extracellular Potential
Transmembrane560 – 58021Helical; Potential
Topological domain581 – 1122542Cytoplasmic Potential
Domain31 – 217187Eph LBD
Domain341 – 445105Fibronectin type-III 1
Domain451 – 53787Fibronectin type-III 2
Domain692 – 988297Protein kinase
Nucleotide binding698 – 7069ATP By similarity UniProtKB Q62413
Motif1120 – 11223PDZ-binding Potential
Compositional bias199 – 338140Cys-rich

Sites

Active site8491Proton acceptor By similarity UniProtKB Q62413
Binding site7481ATP By similarity UniProtKB Q62413

Amino acid modifications

Modified residue6671Phosphotyrosine; by autocatalysis Potential UniProtKB Q62413
Modified residue6731Phosphotyrosine; by autocatalysis Potential UniProtKB Q62413
Modified residue8851Phosphotyrosine; by autocatalysis Potential UniProtKB Q62413
Modified residue10481Phosphotyrosine; by autocatalysis Potential UniProtKB Q62413
Glycosylation1721N-linked (GlcNAc...) Potential
Glycosylation2381N-linked (GlcNAc...) Ref.3
Glycosylation2441N-linked (GlcNAc...) Potential
Glycosylation4991N-linked (GlcNAc...) Potential
Glycosylation5541N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence843 – 8475Missing in isoform b.
VSP_050207

Experimental info

Mutagenesis621E → K in allele ju8; most die as larvae. Ref.1
Mutagenesis631T → I in allele e699; most die as larvae. Ref.1
Mutagenesis1951E → K in allele e856; most die as larvae. Ref.1
Mutagenesis9171G → E in allele e2; most die as adults. Ref.1
Mutagenesis9661C → F in allele ju22; most die as adults. Ref.1
Mutagenesis9661C → Y in allele e1063; most die as adults. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 6E4A3037BB92A1D0

FASTA1,122125,264
        10         20         30         40         50         60 
MRLYNSRILN PHQSIFILVL QCLITIVTSH QEVLFDLSKV GSDLKWDQVS LRHDRDDVWM 

        70         80         90        100        110        120 
EETWRNPAAT DEKHANQRAY VTCNYDMINP SNWLFSHFIE VKTARRIYIE LLFNTRDCDA 

       130        140        150        160        170        180 
YLNPKSCKET FSVYLKQFKT SRPGSTKIEK ERFSEDIDNW KNIGRLARSN SNMTTETLGM 

       190        200        210        220        230        240 
EIDSDTKTIR IAFEEQGICL SLLNVKIYYR ICDEFTDQLV YFRPQVTGPK ETDMVRMNGS 

       250        260        270        280        290        300 
CIPNASKKIP GVDLIGLCMS TGSGIKTSGE CVCDSGYSQI ADSNGARCES CPTNTYKPKG 

       310        320        330        340        350        360 
QSLCKSCPSN SISSEAASSC RCLNGYFRAE DELISMPCTQ PPSRPIKLVA NAITATSTRL 

       370        380        390        400        410        420 
SWNEPSSLGG RPEIWYEVKC SGRGECGTVV MTPGDKKLST RSVQINGLRP SSDYTFLVFA 

       430        440        450        460        470        480 
KNKVSAQFPE FSEKNAVIDI RTRSEEEDVP PVSHLRVDAS QSDGITIAWS VSDSDVSDFE 

       490        500        510        520        530        540 
VEVRPAIVKK RTFETRHVNM TYTTFIGLNP ETVYQFRVRI RDDLRWSQPI SYQLGRGLMS 

       550        560        570        580        590        600 
SPSSNEVEES QFLNQTGSAL LIIIALILIV IAVALCMIVV QKKSKNRKQM SDLDVLDTYK 

       610        620        630        640        650        660 
QDSMTPDYHT TSRHHHHQGN LPATLHEQLR STTKLNAPLI PSFGSPISQP PPYYGGVHPN 

       670        680        690        700        710        720 
SGKYKTYVDP TTYEDPYQAL IEFTFDISPN DVFITQVIGG GEFGDVCLGG LSKNSPAAAK 

       730        740        750        760        770        780 
WSVSNTTMGR GGGGGGYESE PYETVAIKTL KSGSSAKAKA EFLTEATIMG QFSHPNVIRL 

       790        800        810        820        830        840 
IGVVTSAEPV MIVAEYMANG SLDQFLRNTD QRGEKVHWEK ITEMLYGIAS GMKYLTDMGY 

       850        860        870        880        890        900 
VHRVSFLRDL AARNVLLDME LRCKIADFGL SRGVRSEGSS VEPEYTTNGG KIPVRWTAPE 

       910        920        930        940        950        960 
AITHRKFTPS SDVWSFGVVI WEVCSFGERP YWDWTNQKVI SEVMIGYRLP PPMDCPMGLY 

       970        980        990       1000       1010       1020 
RIAQWCWKME RHERPTFTQL LATFHKYILQ PTLIEHDPGE LPRRVQSQSA LNTYGSVNVG 

      1030       1040       1050       1060       1070       1080 
VVPTPPSSAA PMPSLDDFLR QIGLNHVYGQ LVSNNIHSVS DLANTSHLDL LAYGLMSAEC 

      1090       1100       1110       1120 
STVRDGLNGR ISGSPPGSSG TIHATTRGTR TTRPPREEGF FV 

« Hide

Isoform b [UniParc].

Checksum: 6251E5DDB60E7F55
Show »

FASTA1,117124,661

References

« Hide 'large scale' references
[1]"The VAB-1 Eph receptor tyrosine kinase functions in neural and epithelial morphogenesis in C. elegans."
George S.E., Simokat K., Hardin J., Chisholm A.D.
Cell 92:633-643(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF GLU-62; THR-63; GLU-195; GLY-917 AND CYS-966.
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[3]"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-238, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF040269 Genomic DNA. Translation: AAC38970.1.
FO081295 Genomic DNA. Translation: CCD70550.1.
FO081295 Genomic DNA. Translation: CCD70551.1.
PIRT42400.
RefSeqNP_494806.1. NM_062405.5.
NP_494807.1. NM_062406.3.
UniGeneCel.5002.

3D structure databases

ProteinModelPortalO61460.
SMRO61460. Positions 29-521, 644-991.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid39149. 45 interactions.
IntActO61460. 2 interactions.
STRING6239.M03A1.1b.

Proteomic databases

PaxDbO61460.
PRIDEO61460.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaM03A1.1b; M03A1.1b; M03A1.1. [O61460-1]
GeneID173794.
KEGGcel:CELE_M03A1.1.
UCSCM03A1.1a. c. elegans. [O61460-1]

Organism-specific databases

CTD173794.
WormBaseM03A1.1a; CE25060; WBGene00006868; vab-1.
M03A1.1b; CE28617; WBGene00006868; vab-1.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233856.
InParanoidO61460.
KOK05110.
OMAWTTFPES.
OrthoDBEOG7VTDM6.
PhylomeDBO61460.

Enzyme and pathway databases

BRENDA2.7.10.1. 1045.
SignaLinkO61460.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProIPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. Fibronectin_type3.
IPR008979. Galactose-bd-like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamPF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSPR00109. TYRKINASE.
SMARTSM00615. EPH_lbd. 1 hit.
SM00060. FN3. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 1 hit.
PROSITEPS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio881127.

Entry information

Entry nameVAB1_CAEEL
AccessionPrimary (citable) accession number: O61460
Secondary accession number(s): Q21477
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase