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O61460

- VAB1_CAEEL

UniProt

O61460 - VAB1_CAEEL

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Protein

Ephrin receptor 1

Gene

vab-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Receptor for members of the ephrin family. Involved in interactions between neuronal substrate cells and a migrating epithelial sheet in head epidermis morphogenesis. Also required for cell movements following gastrulation and during ventral closure of the epidermis.By similarity1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei748 – 7481ATPPROSITE-ProRule annotation
Active sitei849 – 8491Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi698 – 7069ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein domain specific binding Source: WormBase
  3. protein tyrosine kinase activity Source: WormBase
  4. receptor signaling protein tyrosine kinase activity Source: WormBase
  5. transmembrane receptor protein tyrosine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. axon guidance Source: WormBase
  2. axonogenesis Source: WormBase
  3. embryo development Source: WormBase
  4. embryonic body morphogenesis Source: UniProtKB
  5. ephrin receptor signaling pathway Source: InterPro
  6. morphogenesis of embryonic epithelium Source: UniProtKB
  7. oocyte maturation Source: WormBase
  8. peptidyl-tyrosine phosphorylation Source: WormBase
  9. signal transduction by phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1. 1045.
ReactomeiREACT_230984. EPHB-mediated forward signaling.
REACT_234237. EPH-ephrin mediated repulsion of cells.
REACT_239039. L1CAM interactions.
REACT_247305. EPH-Ephrin signaling.
REACT_252775. Ephrin signaling.
REACT_262526. EPHA-mediated growth cone collapse.
SignaLinkiO61460.

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin receptor 1 (EC:2.7.10.1)
Alternative name(s):
Tyrosine-protein kinase Eph receptor
Variable abnormal protein 1
Gene namesi
Name:vab-1
ORF Names:M03A1.1
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome II

Organism-specific databases

WormBaseiM03A1.1a; CE25060; WBGene00006868; vab-1.
M03A1.1b; CE28617; WBGene00006868; vab-1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 559530ExtracellularSequence AnalysisAdd
BLAST
Transmembranei560 – 58021HelicalSequence AnalysisAdd
BLAST
Topological domaini581 – 1122542CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. axon Source: WormBase
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi62 – 621E → K in allele ju8; most die as larvae. 1 Publication
Mutagenesisi63 – 631T → I in allele e699; most die as larvae. 1 Publication
Mutagenesisi195 – 1951E → K in allele e856; most die as larvae. 1 Publication
Mutagenesisi917 – 9171G → E in allele e2; most die as adults. 1 Publication
Mutagenesisi966 – 9661C → F in allele ju22; most die as adults. 1 Publication
Mutagenesisi966 – 9661C → Y in allele e1063; most die as adults. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence AnalysisAdd
BLAST
Chaini30 – 11221093Ephrin receptor 1PRO_0000016839Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi238 – 2381N-linked (GlcNAc...)1 Publication
Glycosylationi244 – 2441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi499 – 4991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi554 – 5541N-linked (GlcNAc...)Sequence Analysis
Modified residuei667 – 6671Phosphotyrosine; by autocatalysisSequence Analysis
Modified residuei673 – 6731Phosphotyrosine; by autocatalysisSequence Analysis
Modified residuei885 – 8851Phosphotyrosine; by autocatalysisSequence Analysis
Modified residuei1048 – 10481Phosphotyrosine; by autocatalysisSequence Analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO61460.
PRIDEiO61460.

Expressioni

Tissue specificityi

During ventral enclosure of the epidermis, expression is seen in clusters of presumptive head neuronal cells and several cells in the tail region. Early larvae show expression in the nerve ring and ventral nerve cord. Strong expression is also seen in the procorpus and terminal bulb of the pharynx. Expression in the nervous system is seen through to adulthood.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
daf-18G5EE013EBI-1788319,EBI-2914422
vpr-1O447822EBI-1788319,EBI-320991

Protein-protein interaction databases

BioGridi39149. 45 interactions.
IntActiO61460. 2 interactions.
STRINGi6239.M03A1.1b.

Structurei

3D structure databases

ProteinModelPortaliO61460.
SMRiO61460. Positions 29-521, 668-991.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 217187Eph LBDPROSITE-ProRule annotationAdd
BLAST
Domaini341 – 445105Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini451 – 53787Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini692 – 988297Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1120 – 11223PDZ-bindingSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi199 – 338140Cys-richAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation
Contains 1 Eph LBD (Eph ligand-binding) domain.PROSITE-ProRule annotation
Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00740000115264.
HOGENOMiHOG000233856.
InParanoidiO61460.
KOiK05110.
OMAiNEGWEEI.
OrthoDBiEOG7VTDM6.
PhylomeDBiO61460.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProiIPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. Fibronectin_type3.
IPR008979. Galactose-bd-like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00615. EPH_lbd. 1 hit.
SM00060. FN3. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 1 hit.
PROSITEiPS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform aCurated (identifier: O61460-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLYNSRILN PHQSIFILVL QCLITIVTSH QEVLFDLSKV GSDLKWDQVS
60 70 80 90 100
LRHDRDDVWM EETWRNPAAT DEKHANQRAY VTCNYDMINP SNWLFSHFIE
110 120 130 140 150
VKTARRIYIE LLFNTRDCDA YLNPKSCKET FSVYLKQFKT SRPGSTKIEK
160 170 180 190 200
ERFSEDIDNW KNIGRLARSN SNMTTETLGM EIDSDTKTIR IAFEEQGICL
210 220 230 240 250
SLLNVKIYYR ICDEFTDQLV YFRPQVTGPK ETDMVRMNGS CIPNASKKIP
260 270 280 290 300
GVDLIGLCMS TGSGIKTSGE CVCDSGYSQI ADSNGARCES CPTNTYKPKG
310 320 330 340 350
QSLCKSCPSN SISSEAASSC RCLNGYFRAE DELISMPCTQ PPSRPIKLVA
360 370 380 390 400
NAITATSTRL SWNEPSSLGG RPEIWYEVKC SGRGECGTVV MTPGDKKLST
410 420 430 440 450
RSVQINGLRP SSDYTFLVFA KNKVSAQFPE FSEKNAVIDI RTRSEEEDVP
460 470 480 490 500
PVSHLRVDAS QSDGITIAWS VSDSDVSDFE VEVRPAIVKK RTFETRHVNM
510 520 530 540 550
TYTTFIGLNP ETVYQFRVRI RDDLRWSQPI SYQLGRGLMS SPSSNEVEES
560 570 580 590 600
QFLNQTGSAL LIIIALILIV IAVALCMIVV QKKSKNRKQM SDLDVLDTYK
610 620 630 640 650
QDSMTPDYHT TSRHHHHQGN LPATLHEQLR STTKLNAPLI PSFGSPISQP
660 670 680 690 700
PPYYGGVHPN SGKYKTYVDP TTYEDPYQAL IEFTFDISPN DVFITQVIGG
710 720 730 740 750
GEFGDVCLGG LSKNSPAAAK WSVSNTTMGR GGGGGGYESE PYETVAIKTL
760 770 780 790 800
KSGSSAKAKA EFLTEATIMG QFSHPNVIRL IGVVTSAEPV MIVAEYMANG
810 820 830 840 850
SLDQFLRNTD QRGEKVHWEK ITEMLYGIAS GMKYLTDMGY VHRVSFLRDL
860 870 880 890 900
AARNVLLDME LRCKIADFGL SRGVRSEGSS VEPEYTTNGG KIPVRWTAPE
910 920 930 940 950
AITHRKFTPS SDVWSFGVVI WEVCSFGERP YWDWTNQKVI SEVMIGYRLP
960 970 980 990 1000
PPMDCPMGLY RIAQWCWKME RHERPTFTQL LATFHKYILQ PTLIEHDPGE
1010 1020 1030 1040 1050
LPRRVQSQSA LNTYGSVNVG VVPTPPSSAA PMPSLDDFLR QIGLNHVYGQ
1060 1070 1080 1090 1100
LVSNNIHSVS DLANTSHLDL LAYGLMSAEC STVRDGLNGR ISGSPPGSSG
1110 1120
TIHATTRGTR TTRPPREEGF FV
Length:1,122
Mass (Da):125,264
Last modified:August 1, 1998 - v1
Checksum:i6E4A3037BB92A1D0
GO
Isoform bCurated (identifier: O61460-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     843-847: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:1,117
Mass (Da):124,661
Checksum:i6251E5DDB60E7F55
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei843 – 8475Missing in isoform b. CuratedVSP_050207

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF040269 Genomic DNA. Translation: AAC38970.1.
FO081295 Genomic DNA. Translation: CCD70550.1.
FO081295 Genomic DNA. Translation: CCD70551.1.
PIRiT42400.
RefSeqiNP_494806.1. NM_062405.5. [O61460-1]
NP_494807.1. NM_062406.3. [O61460-2]
UniGeneiCel.5002.

Genome annotation databases

EnsemblMetazoaiM03A1.1b; M03A1.1b; WBGene00006868. [O61460-1]
GeneIDi173794.
KEGGicel:CELE_M03A1.1.
UCSCiM03A1.1a. c. elegans. [O61460-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF040269 Genomic DNA. Translation: AAC38970.1 .
FO081295 Genomic DNA. Translation: CCD70550.1 .
FO081295 Genomic DNA. Translation: CCD70551.1 .
PIRi T42400.
RefSeqi NP_494806.1. NM_062405.5. [O61460-1 ]
NP_494807.1. NM_062406.3. [O61460-2 ]
UniGenei Cel.5002.

3D structure databases

ProteinModelPortali O61460.
SMRi O61460. Positions 29-521, 668-991.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 39149. 45 interactions.
IntActi O61460. 2 interactions.
STRINGi 6239.M03A1.1b.

Proteomic databases

PaxDbi O61460.
PRIDEi O61460.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai M03A1.1b ; M03A1.1b ; WBGene00006868 . [O61460-1 ]
GeneIDi 173794.
KEGGi cel:CELE_M03A1.1.
UCSCi M03A1.1a. c. elegans. [O61460-1 ]

Organism-specific databases

CTDi 173794.
WormBasei M03A1.1a ; CE25060 ; WBGene00006868 ; vab-1.
M03A1.1b ; CE28617 ; WBGene00006868 ; vab-1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00740000115264.
HOGENOMi HOG000233856.
InParanoidi O61460.
KOi K05110.
OMAi NEGWEEI.
OrthoDBi EOG7VTDM6.
PhylomeDBi O61460.

Enzyme and pathway databases

BRENDAi 2.7.10.1. 1045.
Reactomei REACT_230984. EPHB-mediated forward signaling.
REACT_234237. EPH-ephrin mediated repulsion of cells.
REACT_239039. L1CAM interactions.
REACT_247305. EPH-Ephrin signaling.
REACT_252775. Ephrin signaling.
REACT_262526. EPHA-mediated growth cone collapse.
SignaLinki O61460.

Miscellaneous databases

NextBioi 881127.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProi IPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. Fibronectin_type3.
IPR008979. Galactose-bd-like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view ]
PRINTSi PR00109. TYRKINASE.
SMARTi SM00615. EPH_lbd. 1 hit.
SM00060. FN3. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 1 hit.
PROSITEi PS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The VAB-1 Eph receptor tyrosine kinase functions in neural and epithelial morphogenesis in C. elegans."
    George S.E., Simokat K., Hardin J., Chisholm A.D.
    Cell 92:633-643(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF GLU-62; THR-63; GLU-195; GLY-917 AND CYS-966.
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
    Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
    Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-238, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiVAB1_CAEEL
AccessioniPrimary (citable) accession number: O61460
Secondary accession number(s): Q21477
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: August 1, 1998
Last modified: November 26, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3