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O61460

- VAB1_CAEEL

UniProt

O61460 - VAB1_CAEEL

Protein

Ephrin receptor 1

Gene

vab-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Receptor for members of the ephrin family. Involved in interactions between neuronal substrate cells and a migrating epithelial sheet in head epidermis morphogenesis. Also required for cell movements following gastrulation and during ventral closure of the epidermis.By similarity1 Publication

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei748 – 7481ATPPROSITE-ProRule annotation
    Active sitei849 – 8491Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi698 – 7069ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein domain specific binding Source: WormBase
    4. protein tyrosine kinase activity Source: WormBase
    5. receptor signaling protein tyrosine kinase activity Source: WormBase
    6. transmembrane receptor protein tyrosine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. axon guidance Source: WormBase
    2. axonogenesis Source: WormBase
    3. embryo development Source: WormBase
    4. embryonic body morphogenesis Source: UniProtKB
    5. ephrin receptor signaling pathway Source: InterPro
    6. morphogenesis of embryonic epithelium Source: UniProtKB
    7. oocyte maturation Source: WormBase
    8. peptidyl-tyrosine phosphorylation Source: WormBase
    9. signal transduction by phosphorylation Source: GOC

    Keywords - Molecular functioni

    Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.10.1. 1045.
    SignaLinkiO61460.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ephrin receptor 1 (EC:2.7.10.1)
    Alternative name(s):
    Tyrosine-protein kinase Eph receptor
    Variable abnormal protein 1
    Gene namesi
    Name:vab-1
    ORF Names:M03A1.1
    OrganismiCaenorhabditis elegansImported
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome II

    Organism-specific databases

    WormBaseiM03A1.1a; CE25060; WBGene00006868; vab-1.
    M03A1.1b; CE28617; WBGene00006868; vab-1.

    Subcellular locationi

    GO - Cellular componenti

    1. axon Source: WormBase
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: WormBase

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi62 – 621E → K in allele ju8; most die as larvae. 1 Publication
    Mutagenesisi63 – 631T → I in allele e699; most die as larvae. 1 Publication
    Mutagenesisi195 – 1951E → K in allele e856; most die as larvae. 1 Publication
    Mutagenesisi917 – 9171G → E in allele e2; most die as adults. 1 Publication
    Mutagenesisi966 – 9661C → F in allele ju22; most die as adults. 1 Publication
    Mutagenesisi966 – 9661C → Y in allele e1063; most die as adults. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Chaini30 – 11221093Ephrin receptor 1PRO_0000016839Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi238 – 2381N-linked (GlcNAc...)1 Publication
    Glycosylationi244 – 2441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi499 – 4991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi554 – 5541N-linked (GlcNAc...)Sequence Analysis
    Modified residuei667 – 6671Phosphotyrosine; by autocatalysisSequence Analysis
    Modified residuei673 – 6731Phosphotyrosine; by autocatalysisSequence Analysis
    Modified residuei885 – 8851Phosphotyrosine; by autocatalysisSequence Analysis
    Modified residuei1048 – 10481Phosphotyrosine; by autocatalysisSequence Analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiO61460.
    PRIDEiO61460.

    Expressioni

    Tissue specificityi

    During ventral enclosure of the epidermis, expression is seen in clusters of presumptive head neuronal cells and several cells in the tail region. Early larvae show expression in the nerve ring and ventral nerve cord. Strong expression is also seen in the procorpus and terminal bulb of the pharynx. Expression in the nervous system is seen through to adulthood.1 Publication

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    daf-18G5EE013EBI-1788319,EBI-2914422
    vpr-1O447822EBI-1788319,EBI-320991

    Protein-protein interaction databases

    BioGridi39149. 45 interactions.
    IntActiO61460. 2 interactions.
    STRINGi6239.M03A1.1b.

    Structurei

    3D structure databases

    ProteinModelPortaliO61460.
    SMRiO61460. Positions 29-521, 668-991, 1032-1082.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini30 – 559530ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini581 – 1122542CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei560 – 58021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 217187Eph LBDPROSITE-ProRule annotationAdd
    BLAST
    Domaini341 – 445105Fibronectin type-III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini451 – 53787Fibronectin type-III 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini692 – 988297Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1120 – 11223PDZ-bindingSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi199 – 338140Cys-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation
    Contains 1 Eph LBD (Eph ligand-binding) domain.PROSITE-ProRule annotation
    Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000115264.
    HOGENOMiHOG000233856.
    InParanoidiO61460.
    KOiK05110.
    OMAiNEGWEEI.
    OrthoDBiEOG7VTDM6.
    PhylomeDBiO61460.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.10. 2 hits.
    InterProiIPR027936. Eph_TM.
    IPR001090. Ephrin_rcpt_lig-bd_dom.
    IPR003961. Fibronectin_type3.
    IPR008979. Galactose-bd-like.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR013783. Ig-like_fold.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF14575. EphA2_TM. 1 hit.
    PF01404. Ephrin_lbd. 1 hit.
    PF00041. fn3. 2 hits.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view]
    PRINTSiPR00109. TYRKINASE.
    SMARTiSM00615. EPH_lbd. 1 hit.
    SM00060. FN3. 2 hits.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49265. SSF49265. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF56112. SSF56112. 1 hit.
    SSF57184. SSF57184. 1 hit.
    PROSITEiPS51550. EPH_LBD. 1 hit.
    PS50853. FN3. 2 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform aCurated (identifier: O61460-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRLYNSRILN PHQSIFILVL QCLITIVTSH QEVLFDLSKV GSDLKWDQVS     50
    LRHDRDDVWM EETWRNPAAT DEKHANQRAY VTCNYDMINP SNWLFSHFIE 100
    VKTARRIYIE LLFNTRDCDA YLNPKSCKET FSVYLKQFKT SRPGSTKIEK 150
    ERFSEDIDNW KNIGRLARSN SNMTTETLGM EIDSDTKTIR IAFEEQGICL 200
    SLLNVKIYYR ICDEFTDQLV YFRPQVTGPK ETDMVRMNGS CIPNASKKIP 250
    GVDLIGLCMS TGSGIKTSGE CVCDSGYSQI ADSNGARCES CPTNTYKPKG 300
    QSLCKSCPSN SISSEAASSC RCLNGYFRAE DELISMPCTQ PPSRPIKLVA 350
    NAITATSTRL SWNEPSSLGG RPEIWYEVKC SGRGECGTVV MTPGDKKLST 400
    RSVQINGLRP SSDYTFLVFA KNKVSAQFPE FSEKNAVIDI RTRSEEEDVP 450
    PVSHLRVDAS QSDGITIAWS VSDSDVSDFE VEVRPAIVKK RTFETRHVNM 500
    TYTTFIGLNP ETVYQFRVRI RDDLRWSQPI SYQLGRGLMS SPSSNEVEES 550
    QFLNQTGSAL LIIIALILIV IAVALCMIVV QKKSKNRKQM SDLDVLDTYK 600
    QDSMTPDYHT TSRHHHHQGN LPATLHEQLR STTKLNAPLI PSFGSPISQP 650
    PPYYGGVHPN SGKYKTYVDP TTYEDPYQAL IEFTFDISPN DVFITQVIGG 700
    GEFGDVCLGG LSKNSPAAAK WSVSNTTMGR GGGGGGYESE PYETVAIKTL 750
    KSGSSAKAKA EFLTEATIMG QFSHPNVIRL IGVVTSAEPV MIVAEYMANG 800
    SLDQFLRNTD QRGEKVHWEK ITEMLYGIAS GMKYLTDMGY VHRVSFLRDL 850
    AARNVLLDME LRCKIADFGL SRGVRSEGSS VEPEYTTNGG KIPVRWTAPE 900
    AITHRKFTPS SDVWSFGVVI WEVCSFGERP YWDWTNQKVI SEVMIGYRLP 950
    PPMDCPMGLY RIAQWCWKME RHERPTFTQL LATFHKYILQ PTLIEHDPGE 1000
    LPRRVQSQSA LNTYGSVNVG VVPTPPSSAA PMPSLDDFLR QIGLNHVYGQ 1050
    LVSNNIHSVS DLANTSHLDL LAYGLMSAEC STVRDGLNGR ISGSPPGSSG 1100
    TIHATTRGTR TTRPPREEGF FV 1122
    Length:1,122
    Mass (Da):125,264
    Last modified:August 1, 1998 - v1
    Checksum:i6E4A3037BB92A1D0
    GO
    Isoform bCurated (identifier: O61460-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         843-847: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:1,117
    Mass (Da):124,661
    Checksum:i6251E5DDB60E7F55
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei843 – 8475Missing in isoform b. CuratedVSP_050207

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF040269 Genomic DNA. Translation: AAC38970.1.
    FO081295 Genomic DNA. Translation: CCD70550.1.
    FO081295 Genomic DNA. Translation: CCD70551.1.
    PIRiT42400.
    RefSeqiNP_494806.1. NM_062405.5. [O61460-1]
    NP_494807.1. NM_062406.3. [O61460-2]
    UniGeneiCel.5002.

    Genome annotation databases

    EnsemblMetazoaiM03A1.1b; M03A1.1b; WBGene00006868. [O61460-1]
    GeneIDi173794.
    KEGGicel:CELE_M03A1.1.
    UCSCiM03A1.1a. c. elegans. [O61460-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF040269 Genomic DNA. Translation: AAC38970.1 .
    FO081295 Genomic DNA. Translation: CCD70550.1 .
    FO081295 Genomic DNA. Translation: CCD70551.1 .
    PIRi T42400.
    RefSeqi NP_494806.1. NM_062405.5. [O61460-1 ]
    NP_494807.1. NM_062406.3. [O61460-2 ]
    UniGenei Cel.5002.

    3D structure databases

    ProteinModelPortali O61460.
    SMRi O61460. Positions 29-521, 668-991, 1032-1082.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 39149. 45 interactions.
    IntActi O61460. 2 interactions.
    STRINGi 6239.M03A1.1b.

    Proteomic databases

    PaxDbi O61460.
    PRIDEi O61460.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai M03A1.1b ; M03A1.1b ; WBGene00006868 . [O61460-1 ]
    GeneIDi 173794.
    KEGGi cel:CELE_M03A1.1.
    UCSCi M03A1.1a. c. elegans. [O61460-1 ]

    Organism-specific databases

    CTDi 173794.
    WormBasei M03A1.1a ; CE25060 ; WBGene00006868 ; vab-1.
    M03A1.1b ; CE28617 ; WBGene00006868 ; vab-1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000115264.
    HOGENOMi HOG000233856.
    InParanoidi O61460.
    KOi K05110.
    OMAi NEGWEEI.
    OrthoDBi EOG7VTDM6.
    PhylomeDBi O61460.

    Enzyme and pathway databases

    BRENDAi 2.7.10.1. 1045.
    SignaLinki O61460.

    Miscellaneous databases

    NextBioi 881127.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.10. 2 hits.
    InterProi IPR027936. Eph_TM.
    IPR001090. Ephrin_rcpt_lig-bd_dom.
    IPR003961. Fibronectin_type3.
    IPR008979. Galactose-bd-like.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR013783. Ig-like_fold.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF14575. EphA2_TM. 1 hit.
    PF01404. Ephrin_lbd. 1 hit.
    PF00041. fn3. 2 hits.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view ]
    PRINTSi PR00109. TYRKINASE.
    SMARTi SM00615. EPH_lbd. 1 hit.
    SM00060. FN3. 2 hits.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49265. SSF49265. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF56112. SSF56112. 1 hit.
    SSF57184. SSF57184. 1 hit.
    PROSITEi PS51550. EPH_LBD. 1 hit.
    PS50853. FN3. 2 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The VAB-1 Eph receptor tyrosine kinase functions in neural and epithelial morphogenesis in C. elegans."
      George S.E., Simokat K., Hardin J., Chisholm A.D.
      Cell 92:633-643(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF GLU-62; THR-63; GLU-195; GLY-917 AND CYS-966.
      Strain: Bristol N2.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    3. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
      Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
      Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-238, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiVAB1_CAEEL
    AccessioniPrimary (citable) accession number: O61460
    Secondary accession number(s): Q21477
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2003
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3