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Protein

Ephrin receptor 1

Gene

vab-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for members of the ephrin family. Involved in interactions between neuronal substrate cells and a migrating epithelial sheet in head epidermis morphogenesis. Also required for cell movements following gastrulation and during ventral closure of the epidermis.By similarity1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei748ATPPROSITE-ProRule annotation1
Active sitei849Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi698 – 706ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ephrin receptor activity Source: WormBase
  • protein domain specific binding Source: WormBase
  • protein tyrosine kinase activity Source: WormBase
  • receptor signaling protein tyrosine kinase activity Source: WormBase

GO - Biological processi

  • axon guidance Source: WormBase
  • axonogenesis Source: WormBase
  • embryo development ending in birth or egg hatching Source: WormBase
  • embryonic body morphogenesis Source: UniProtKB
  • morphogenesis of embryonic epithelium Source: UniProtKB
  • oocyte maturation Source: WormBase
  • peptidyl-tyrosine phosphorylation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1. 1045.
ReactomeiR-CEL-3928663. EPHA-mediated growth cone collapse.
SignaLinkiO61460.

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin receptor 1 (EC:2.7.10.1)
Alternative name(s):
Tyrosine-protein kinase Eph receptor
Variable abnormal protein 1
Gene namesi
Name:vab-1
ORF Names:M03A1.1
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiM03A1.1a; CE25060; WBGene00006868; vab-1.
M03A1.1b; CE28617; WBGene00006868; vab-1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 559ExtracellularSequence analysisAdd BLAST530
Transmembranei560 – 580HelicalSequence analysisAdd BLAST21
Topological domaini581 – 1122CytoplasmicSequence analysisAdd BLAST542

GO - Cellular componenti

  • axon Source: WormBase
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi62E → K in allele ju8; most die as larvae. 1 Publication1
Mutagenesisi63T → I in allele e699; most die as larvae. 1 Publication1
Mutagenesisi195E → K in allele e856; most die as larvae. 1 Publication1
Mutagenesisi917G → E in allele e2; most die as adults. 1 Publication1
Mutagenesisi966C → F in allele ju22; most die as adults. 1 Publication1
Mutagenesisi966C → Y in allele e1063; most die as adults. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000001683930 – 1122Ephrin receptor 1Add BLAST1093

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi172N-linked (GlcNAc...)Sequence analysis1
Glycosylationi238N-linked (GlcNAc...)1 Publication1
Glycosylationi244N-linked (GlcNAc...)Sequence analysis1
Glycosylationi499N-linked (GlcNAc...)Sequence analysis1
Glycosylationi554N-linked (GlcNAc...)Sequence analysis1
Modified residuei667Phosphotyrosine; by autocatalysisSequence analysis1
Modified residuei673Phosphotyrosine; by autocatalysisSequence analysis1
Modified residuei885Phosphotyrosine; by autocatalysisSequence analysis1
Modified residuei1048Phosphotyrosine; by autocatalysisSequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO61460.
PeptideAtlasiO61460.
PRIDEiO61460.

Expressioni

Tissue specificityi

During ventral enclosure of the epidermis, expression is seen in clusters of presumptive head neuronal cells and several cells in the tail region. Early larvae show expression in the nerve ring and ventral nerve cord. Strong expression is also seen in the procorpus and terminal bulb of the pharynx. Expression in the nervous system is seen through to adulthood.1 Publication

Gene expression databases

BgeeiWBGene00006868.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
daf-18G5EE013EBI-1788319,EBI-2914422
vpr-1O447822EBI-1788319,EBI-320991

GO - Molecular functioni

  • protein domain specific binding Source: WormBase

Protein-protein interaction databases

BioGridi39149. 45 interactors.
IntActiO61460. 2 interactors.
STRINGi6239.M03A1.1b.

Structurei

3D structure databases

ProteinModelPortaliO61460.
SMRiO61460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 217Eph LBDPROSITE-ProRule annotationAdd BLAST187
Domaini341 – 445Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST105
Domaini451 – 537Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST87
Domaini692 – 988Protein kinasePROSITE-ProRule annotationAdd BLAST297

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1120 – 1122PDZ-bindingSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi199 – 338Cys-richAdd BLAST140

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation
Contains 1 Eph LBD (Eph ligand-binding) domain.PROSITE-ProRule annotation
Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0196. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00740000115264.
HOGENOMiHOG000233856.
InParanoidiO61460.
KOiK05110.
OMAiPTFLCKG.
OrthoDBiEOG091G00W0.
PhylomeDBiO61460.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProiIPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. FN3_dom.
IPR008979. Galactose-bd-like.
IPR009030. Growth_fac_rcpt_.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR011641. Tyr-kin_ephrin_A/B_rcpt-like.
[Graphical view]
PfamiPF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00615. EPH_lbd. 1 hit.
SM01411. Ephrin_rec_like. 1 hit.
SM00060. FN3. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 1 hit.
PROSITEiPS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform aCurated (identifier: O61460-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLYNSRILN PHQSIFILVL QCLITIVTSH QEVLFDLSKV GSDLKWDQVS
60 70 80 90 100
LRHDRDDVWM EETWRNPAAT DEKHANQRAY VTCNYDMINP SNWLFSHFIE
110 120 130 140 150
VKTARRIYIE LLFNTRDCDA YLNPKSCKET FSVYLKQFKT SRPGSTKIEK
160 170 180 190 200
ERFSEDIDNW KNIGRLARSN SNMTTETLGM EIDSDTKTIR IAFEEQGICL
210 220 230 240 250
SLLNVKIYYR ICDEFTDQLV YFRPQVTGPK ETDMVRMNGS CIPNASKKIP
260 270 280 290 300
GVDLIGLCMS TGSGIKTSGE CVCDSGYSQI ADSNGARCES CPTNTYKPKG
310 320 330 340 350
QSLCKSCPSN SISSEAASSC RCLNGYFRAE DELISMPCTQ PPSRPIKLVA
360 370 380 390 400
NAITATSTRL SWNEPSSLGG RPEIWYEVKC SGRGECGTVV MTPGDKKLST
410 420 430 440 450
RSVQINGLRP SSDYTFLVFA KNKVSAQFPE FSEKNAVIDI RTRSEEEDVP
460 470 480 490 500
PVSHLRVDAS QSDGITIAWS VSDSDVSDFE VEVRPAIVKK RTFETRHVNM
510 520 530 540 550
TYTTFIGLNP ETVYQFRVRI RDDLRWSQPI SYQLGRGLMS SPSSNEVEES
560 570 580 590 600
QFLNQTGSAL LIIIALILIV IAVALCMIVV QKKSKNRKQM SDLDVLDTYK
610 620 630 640 650
QDSMTPDYHT TSRHHHHQGN LPATLHEQLR STTKLNAPLI PSFGSPISQP
660 670 680 690 700
PPYYGGVHPN SGKYKTYVDP TTYEDPYQAL IEFTFDISPN DVFITQVIGG
710 720 730 740 750
GEFGDVCLGG LSKNSPAAAK WSVSNTTMGR GGGGGGYESE PYETVAIKTL
760 770 780 790 800
KSGSSAKAKA EFLTEATIMG QFSHPNVIRL IGVVTSAEPV MIVAEYMANG
810 820 830 840 850
SLDQFLRNTD QRGEKVHWEK ITEMLYGIAS GMKYLTDMGY VHRVSFLRDL
860 870 880 890 900
AARNVLLDME LRCKIADFGL SRGVRSEGSS VEPEYTTNGG KIPVRWTAPE
910 920 930 940 950
AITHRKFTPS SDVWSFGVVI WEVCSFGERP YWDWTNQKVI SEVMIGYRLP
960 970 980 990 1000
PPMDCPMGLY RIAQWCWKME RHERPTFTQL LATFHKYILQ PTLIEHDPGE
1010 1020 1030 1040 1050
LPRRVQSQSA LNTYGSVNVG VVPTPPSSAA PMPSLDDFLR QIGLNHVYGQ
1060 1070 1080 1090 1100
LVSNNIHSVS DLANTSHLDL LAYGLMSAEC STVRDGLNGR ISGSPPGSSG
1110 1120
TIHATTRGTR TTRPPREEGF FV
Length:1,122
Mass (Da):125,264
Last modified:August 1, 1998 - v1
Checksum:i6E4A3037BB92A1D0
GO
Isoform bCurated (identifier: O61460-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     843-847: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:1,117
Mass (Da):124,661
Checksum:i6251E5DDB60E7F55
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050207843 – 847Missing in isoform b. Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF040269 Genomic DNA. Translation: AAC38970.1.
FO081295 Genomic DNA. Translation: CCD70550.1.
FO081295 Genomic DNA. Translation: CCD70551.1.
PIRiT42400.
RefSeqiNP_494806.1. NM_062405.5. [O61460-1]
NP_494807.1. NM_062406.3. [O61460-2]
UniGeneiCel.5002.

Genome annotation databases

EnsemblMetazoaiM03A1.1b; M03A1.1b; WBGene00006868. [O61460-1]
GeneIDi173794.
KEGGicel:CELE_M03A1.1.
UCSCiM03A1.1a. c. elegans. [O61460-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF040269 Genomic DNA. Translation: AAC38970.1.
FO081295 Genomic DNA. Translation: CCD70550.1.
FO081295 Genomic DNA. Translation: CCD70551.1.
PIRiT42400.
RefSeqiNP_494806.1. NM_062405.5. [O61460-1]
NP_494807.1. NM_062406.3. [O61460-2]
UniGeneiCel.5002.

3D structure databases

ProteinModelPortaliO61460.
SMRiO61460.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi39149. 45 interactors.
IntActiO61460. 2 interactors.
STRINGi6239.M03A1.1b.

Proteomic databases

PaxDbiO61460.
PeptideAtlasiO61460.
PRIDEiO61460.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiM03A1.1b; M03A1.1b; WBGene00006868. [O61460-1]
GeneIDi173794.
KEGGicel:CELE_M03A1.1.
UCSCiM03A1.1a. c. elegans. [O61460-1]

Organism-specific databases

CTDi173794.
WormBaseiM03A1.1a; CE25060; WBGene00006868; vab-1.
M03A1.1b; CE28617; WBGene00006868; vab-1.

Phylogenomic databases

eggNOGiKOG0196. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00740000115264.
HOGENOMiHOG000233856.
InParanoidiO61460.
KOiK05110.
OMAiPTFLCKG.
OrthoDBiEOG091G00W0.
PhylomeDBiO61460.

Enzyme and pathway databases

BRENDAi2.7.10.1. 1045.
ReactomeiR-CEL-3928663. EPHA-mediated growth cone collapse.
SignaLinkiO61460.

Miscellaneous databases

PROiO61460.

Gene expression databases

BgeeiWBGene00006868.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProiIPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. FN3_dom.
IPR008979. Galactose-bd-like.
IPR009030. Growth_fac_rcpt_.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR011641. Tyr-kin_ephrin_A/B_rcpt-like.
[Graphical view]
PfamiPF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00615. EPH_lbd. 1 hit.
SM01411. Ephrin_rec_like. 1 hit.
SM00060. FN3. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 1 hit.
PROSITEiPS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVAB1_CAEEL
AccessioniPrimary (citable) accession number: O61460
Secondary accession number(s): Q21477
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.