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Reviewed, UniProtKB/Swiss-Prot O61394 (GALT6_CAEEL)

Last modified June 16, 2009. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Probable N-acetylgalactosaminyltransferase 6
    EC=2.4.1.-
Alternative name(s):
    Protein-UDP acetylgalactosaminyltransferase 6
    UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 6
      Short name=pp-GaNTase 6
Gene names
Name: gly-6
ORF Names: H38K22.5
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length618 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Probable glycopeptide transferase involved in O-linked oligosaccharide biosynthesis. Glycopeptide transferases catalyze the transfer of an N-acetyl-D-galactosamine residue to an already glycosylated peptide By similarity. In contrast to other members of the family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on peptides that have been tested. Some peptide transferase activity is however not excluded, considering that its appropriate peptide substrate may remain unidentified.

Cofactor

Manganese By similarity.

Calcium By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Domain

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity.

The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.

Sequence similarities

Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.

Contains 1 ricin B-type lectin domain.

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: O61394-1)

Also known as: GLY6a; GLY-6a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: O61394-2)

Also known as: GLY6b; GLY-6b;

The sequence of this isoform differs from the canonical sequence as follows:
     479-512: TSSSNSSVCLAWTLRSSGIKTASTADCLKIFHKT → SNSNYCTAFRPGDTGPKNHRLLGSPCTMGFDLW
Isoform c (identifier: O61394-3)

Also known as: GLY6c; GLY-6c;

The sequence of this isoform differs from the canonical sequence as follows:
     495-618: SGIKTASTAD...TEMSWLPEHP → CPTQTTAQPS...FTQLPIGKFN

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 618618Probable N-acetylgalactosaminyltransferase 6
PRO_0000059149

Regions

Topological domain1 – 1616Cytoplasmic Potential
Transmembrane17 – 3923Signal-anchor for type II membrane protein Potential
Topological domain40 – 618579Lumenal Potential
Domain474 – 609136Ricin B-type lectin
Region156 – 267112Catalytic subdomain A
Region327 – 38963Catalytic subdomain B

Amino acid modifications

Glycosylation811N-linked (GlcNAc...) Potential
Glycosylation1491N-linked (GlcNAc...) Potential
Glycosylation4831N-linked (GlcNAc...) Potential
Glycosylation6051N-linked (GlcNAc...) Potential
Disulfide bond487 ↔ 505 By similarity
Disulfide bond530 ↔ 550 By similarity
Disulfide bond575 ↔ 597 By similarity

Natural variations

Alternative sequence479 – 51234TSSSN…IFHKT → SNSNYCTAFRPGDTGPKNHR LLGSPCTMGFDLW in isoform b.
VSP_011240
Alternative sequence495 – 618124SGIKT…LPEHP → CPTQTTAQPSGQVTRVPKIT DCSDHPVLWDSTCGSCGSTL ATVEYVQMSIYAYQLFNFFT QLPIGKFN in isoform c.
VSP_011241

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Sequences

Sequence LengthMass (Da)Tools
Isoform a (GLY6a) (GLY-6a) [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 738A01F7BB445E22

FASTA61871,115
        10         20         30         40         50         60 
MIASLIRSRR RSRRCVVYSV FLFGFLALWG SFALALVFLS DMYIGEDQIS TQKAIKPIAR 

        70         80         90        100        110        120 
SNYHVVVGHY NGNLPEDKKR NLTSEELNAN LYAPHDDWGE GGAGVSHLTP EQQKLADSTF 

       130        140        150        160        170        180 
AVNQFNLLVS DGISVRRSLP EIRKPSCRNM TYPDNLPTTS VIIVYHNEAY STLLRTVWSV 

       190        200        210        220        230        240 
IDRSPKELLK EIILVDDFSD REFLRYPTLD TTLKPLPTDI KIIRSKERVG LIRARMMGAQ 

       250        260        270        280        290        300 
EAQGDVLTFL DSHCECTKGW LEPLLTRIKL NRKAVPCPVI DIINDNTFQY QKGIEMFRGG 

       310        320        330        340        350        360 
FNWNLQFRWY GMPTAMAKQH LLDPTGPIES PTMAGGLFSI NRNYFEELGE YDPGMDIWGG 

       370        380        390        400        410        420 
ENLEMSFRIW QCGGRVEILP CSHVGHVFRK SSPHDFPGKS SGKVLNTNLL RVAEVWMDDW 

       430        440        450        460        470        480 
KHYFYKIAPQ AHRMRSSIDV SERVELRKKL NCKSFKWYLQ NVFQDHFLPT PLDRFGRMTS 

       490        500        510        520        530        540 
SSNSSVCLAW TLRSSGIKTA STADCLKIFH KTQLWLYTGD RRIRTDEHLC LSVVQLLHTT 

       550        560        570        580        590        600 
SDWKIQLKEC AGFDTEYWDF KPKIGRFQNR KTGLCLASPD IFDPTKDEFN PPIVQKCRSS 

       610 
NDRQNWTITE MSWLPEHP

« Hide

Isoform b (GLY6b) (GLY-6b).

Checksum: FA096FFBC27222F1
Show »

FASTA61771,157
Isoform c (GLY6c) (GLY-6c).

Checksum: CCB49C0E1DB6014B
Show »

FASTA56264,124

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References

« Hide 'large scale' references
[1]"cDNA cloning and expression of a family of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase sequence homologs from Caenorhabditis elegans."
Hagen F.K., Nehrke K.
J. Biol. Chem. 273:8268-8277(1998) [PubMed: 9525933] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND C).
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.

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Cross-references

Sequence databases

AF031838 mRNA. Translation: AAC13674.1.
AF031839 mRNA. Translation: AAC13675.1.
AF031840 mRNA. Translation: AAC13676.1.
AL024499 Genomic DNA. Translation: CAA19707.1.
AL024499 Genomic DNA. Translation: CAC42317.1.
AL024499 Genomic DNA. Translation: CAC42318.1.
PIRT42248.
T42249.
T42250.
RefSeqNP_001022644.1.
UniGeneCel.8088

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Proteomic databases

PRIDEO61394.

Genome annotation databases

EnsemblH38K22.5. Caenorhabditis elegans. [Contig view]
GeneID175558.
KEGGcel:H38K22.5.

Organism-specific databases

WormBaseWBGene00001631. gly-6.
WormPepH38K22.5a. CE18833. [WorfDB]
H38K22.5b. CE28040. [WorfDB]
H38K22.5c. CE28041. [WorfDB]

Phylogenomic databases

OMAO61394. NLQFRWY.

Gene expression databases

ArrayExpressO61394.

Family and domain databases

InterProIPR001173. Glyco_trans_2.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTSM00458. RICIN. 1 hit.
[Graphical view]
PROSITEPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio888666.

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Entry information

Entry nameGALT6_CAEEL
AccessionPrimary (citable) accession number: O61394
Secondary accession number(s): O61395, O61396
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 1, 1998
Last modified: June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents