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O61309

- NOS_LYMST

UniProt

O61309 - NOS_LYMST

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Protein
Nitric oxide synthase
Gene
NOS
Organism
Lymnaea stagnalis (Great pond snail) (Helix stagnalis)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body By similarity.

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Heme group By similarity.
Binds 1 FAD By similarity.
Binds 1 FMN By similarity.

Enzyme regulationi

Stimulated by calcium/calmodulin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi82 – 821Iron (heme axial ligand) By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi556 – 58732FMN By similarity
Add
BLAST
Nucleotide bindingi697 – 70812FAD By similarity
Add
BLAST
Nucleotide bindingi836 – 84611FAD By similarity
Add
BLAST
Nucleotide bindingi911 – 92919NADP By similarity
Add
BLAST
Nucleotide bindingi1089 – 110416NADP By similarity
Add
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. NADP binding Source: InterPro
  3. flavin adenine dinucleotide binding Source: InterPro
  4. heme binding Source: InterPro
  5. iron ion binding Source: InterPro
  6. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase (EC:1.14.13.39)
Alternative name(s):
NOS type I
Neuronal NOS
Short name:
N-NOS
Short name:
nNOS
Gene namesi
Name:NOS
OrganismiLymnaea stagnalis (Great pond snail) (Helix stagnalis)
Taxonomic identifieri6523 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaHeterobranchiaEuthyneuraPanpulmonataHygrophilaLymnaeoideaLymnaeidaeLymnaea

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11531153Nitric oxide synthase
PRO_0000170949Add
BLAST

Expressioni

Tissue specificityi

Expressed in the central nervous system, in the serotonergic cerebral giant cells. The isoform Long and isoform Short are expressed equally in the CNS.

Structurei

3D structure databases

ProteinModelPortaliO61309.
SMRiO61309. Positions 16-384.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini427 – 610184Flavodoxin-like
Add
BLAST
Domaini660 – 903244FAD-binding FR-type
Add
BLAST
Repeati934 – 94071
Repeati941 – 94772
Repeati948 – 95473
Repeati955 – 96174
Repeati962 – 96875
Repeati969 – 97576
Repeati976 – 98277
Repeati983 – 98978
Repeati990 – 99679
Repeati997 – 1003710
Repeati1004 – 1010711

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni397 – 41721Calmodulin-binding Reviewed prediction
Add
BLAST
Regioni934 – 10107711 X 7 AA tandem repeats of E-[NTR]-[ST]-[IM]-[PLQ]-[SP]-[CW]
Add
BLAST

Sequence similaritiesi

Belongs to the NOS family.

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: O61309-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGSLSQQAHG PPDAPRSKEE LLIHAKDFIN QYFTSFQMNK TRAHFHRLGE     50
INDLIEKSGT YDLTMAELTF GAKHAWRNAP GCIGRSQWSK LQVFDAREIG 100
TPREMFEALC SHIRYATNEG KIRSTITIFP QRKEGRPDFR VWNTQLISYA 150
GYKLGDGKVI GDPANVEFTE MCVEMGWKPK HGMFDLLPLV LSAAENSPEY 200
FELPTELVLE VTLKHPEYPW FAEMGLKWYA LPTDSGMLLD CGGLEFPSCP 250
FNGWFMGTMI GSRNLCDPHR YNMLEPIGLK MGLNTETASS LWKDRVLIEV 300
NVAVLYSFES ANVTIVNHHD ASTDFISHMD KEIKLRGGCP SDWVRMVPPM 350
SGSTLEVFHQ EMLLYNLHPA FVRQDVKPWK KHVWKSDQSV PINSCNPKRK 400
LGFKALARAV EFSASLMSKA LSSRVKCSIF YATETGRSER FARRLSEIFK 450
PVFHSRVVCM DDYAVETLEH ESLVMVITST FGNGEPPENG KQFAQSLLDM 500
KRKYDCDLGF LESCSSISTC IKSSILTEGP LAADVIGDRQ SLAMGTGPLC 550
NVRFAVFGLG SKAYPYYAAY GKYIYLMLQE LGAERLVNYC AGDALYGQEQ 600
SFRAWSEEVF KASCEAFCLD NRNDAPGPQT KGDCSKVRIV PVENCQEPDL 650
CQVLRNIHGK EVMPLILAER IQLQAKDSDQ QTILIKLDAH NATDLKYAPG 700
DHVAIFPANS PEIVDAILVR LDTSKGPSPD QVVKTEISTQ LGTNDTWRSH 750
LPICTSRTAF SFLLDVTTPP SQEILQVLAT QASSDMDKHK LEQLASNSEA 800
YEKWRLDLSP NILEILDEFP SLKIPPSLLL TQLPLLQPRY YSISSSQQKN 850
PNEVHATIAV VRFKTQDGDG PVHEGVCSSW LNRSPIGTVV PCFLRSAPHF 900
HLPEDPSLPI IMIGPGSGIA PFRSFWQQRL GEIENTMPSC ENTMLSCETT 950
IPSCENSMPS CENTMPSCEN TMPSCENTIP SCENTIPSCE NTMPSCENTI 1000
PSWERTMQPC QIILPSQTKK HFGEMVLYTG CRTAKHMIYA AELEEMKRLG 1050
VLSNYHVALS REAALPKMYV QDIIIKNAAA VYEIVMKKGG HFYVSGDVSM 1100
AHDVTRALEL VLCQQGGREA SQQVMSLRDE NLFHEDIFGS FVRKAGGQRS 1150
EDE 1153
Length:1,153
Mass (Da):129,086
Last modified:August 1, 1998 - v1
Checksum:i101B77D02B66B109
GO
Isoform Short (identifier: O61309-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     276-309: Missing.

Show »
Length:1,119
Mass (Da):125,309
Checksum:iF3882D2B9296497D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei276 – 30934Missing in isoform Short.
VSP_003584Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012531 mRNA. Translation: AAC17487.1.
PIRiT31080.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012531 mRNA. Translation: AAC17487.1 .
PIRi T31080.

3D structure databases

ProteinModelPortali O61309.
SMRi O61309. Positions 16-384.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000333. NOS. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of NOS in a mollusc: expression in a giant modulatory neuron."
    Korneev S.A., Piper M.R., Picot J., Phillips R., Korneeva E.I., O'Shea M.
    J. Neurobiol. 35:65-76(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    Tissue: CNS.

Entry informationi

Entry nameiNOS_LYMST
AccessioniPrimary (citable) accession number: O61309
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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