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O61309

- NOS_LYMST

UniProt

O61309 - NOS_LYMST

Protein

Nitric oxide synthase

Gene

NOS

Organism
Lymnaea stagnalis (Great pond snail) (Helix stagnalis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body.By similarity

    Catalytic activityi

    2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

    Cofactori

    Heme group.By similarity
    Binds 1 FAD.By similarity
    Binds 1 FMN.By similarity

    Enzyme regulationi

    Stimulated by calcium/calmodulin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi82 – 821Iron (heme axial ligand)By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi556 – 58732FMNPROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi697 – 70812FADBy similarityAdd
    BLAST
    Nucleotide bindingi836 – 84611FADBy similarityAdd
    BLAST
    Nucleotide bindingi911 – 92919NADPBy similarityAdd
    BLAST
    Nucleotide bindingi1089 – 110416NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. FMN binding Source: InterPro
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro
    5. NADP binding Source: InterPro
    6. nitric-oxide synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitric oxide biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitric oxide synthase (EC:1.14.13.39)
    Alternative name(s):
    NOS type I
    Neuronal NOS
    Short name:
    N-NOS
    Short name:
    nNOS
    Gene namesi
    Name:NOS
    OrganismiLymnaea stagnalis (Great pond snail) (Helix stagnalis)
    Taxonomic identifieri6523 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaHeterobranchiaEuthyneuraPanpulmonataHygrophilaLymnaeoideaLymnaeidaeLymnaea

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11531153Nitric oxide synthasePRO_0000170949Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in the central nervous system, in the serotonergic cerebral giant cells. The isoform Long and isoform Short are expressed equally in the CNS.

    Structurei

    3D structure databases

    ProteinModelPortaliO61309.
    SMRiO61309. Positions 16-384.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini427 – 610184Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini660 – 903244FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST
    Repeati934 – 94071
    Repeati941 – 94772
    Repeati948 – 95473
    Repeati955 – 96174
    Repeati962 – 96875
    Repeati969 – 97576
    Repeati976 – 98277
    Repeati983 – 98978
    Repeati990 – 99679
    Repeati997 – 1003710
    Repeati1004 – 1010711

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni397 – 41721Calmodulin-bindingSequence AnalysisAdd
    BLAST
    Regioni934 – 10107711 X 7 AA tandem repeats of E-[NTR]-[ST]-[IM]-[PLQ]-[SP]-[CW]Add
    BLAST

    Sequence similaritiesi

    Belongs to the NOS family.Curated
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 2 hits.
    3.90.340.10. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000333. NOS. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: O61309-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGSLSQQAHG PPDAPRSKEE LLIHAKDFIN QYFTSFQMNK TRAHFHRLGE     50
    INDLIEKSGT YDLTMAELTF GAKHAWRNAP GCIGRSQWSK LQVFDAREIG 100
    TPREMFEALC SHIRYATNEG KIRSTITIFP QRKEGRPDFR VWNTQLISYA 150
    GYKLGDGKVI GDPANVEFTE MCVEMGWKPK HGMFDLLPLV LSAAENSPEY 200
    FELPTELVLE VTLKHPEYPW FAEMGLKWYA LPTDSGMLLD CGGLEFPSCP 250
    FNGWFMGTMI GSRNLCDPHR YNMLEPIGLK MGLNTETASS LWKDRVLIEV 300
    NVAVLYSFES ANVTIVNHHD ASTDFISHMD KEIKLRGGCP SDWVRMVPPM 350
    SGSTLEVFHQ EMLLYNLHPA FVRQDVKPWK KHVWKSDQSV PINSCNPKRK 400
    LGFKALARAV EFSASLMSKA LSSRVKCSIF YATETGRSER FARRLSEIFK 450
    PVFHSRVVCM DDYAVETLEH ESLVMVITST FGNGEPPENG KQFAQSLLDM 500
    KRKYDCDLGF LESCSSISTC IKSSILTEGP LAADVIGDRQ SLAMGTGPLC 550
    NVRFAVFGLG SKAYPYYAAY GKYIYLMLQE LGAERLVNYC AGDALYGQEQ 600
    SFRAWSEEVF KASCEAFCLD NRNDAPGPQT KGDCSKVRIV PVENCQEPDL 650
    CQVLRNIHGK EVMPLILAER IQLQAKDSDQ QTILIKLDAH NATDLKYAPG 700
    DHVAIFPANS PEIVDAILVR LDTSKGPSPD QVVKTEISTQ LGTNDTWRSH 750
    LPICTSRTAF SFLLDVTTPP SQEILQVLAT QASSDMDKHK LEQLASNSEA 800
    YEKWRLDLSP NILEILDEFP SLKIPPSLLL TQLPLLQPRY YSISSSQQKN 850
    PNEVHATIAV VRFKTQDGDG PVHEGVCSSW LNRSPIGTVV PCFLRSAPHF 900
    HLPEDPSLPI IMIGPGSGIA PFRSFWQQRL GEIENTMPSC ENTMLSCETT 950
    IPSCENSMPS CENTMPSCEN TMPSCENTIP SCENTIPSCE NTMPSCENTI 1000
    PSWERTMQPC QIILPSQTKK HFGEMVLYTG CRTAKHMIYA AELEEMKRLG 1050
    VLSNYHVALS REAALPKMYV QDIIIKNAAA VYEIVMKKGG HFYVSGDVSM 1100
    AHDVTRALEL VLCQQGGREA SQQVMSLRDE NLFHEDIFGS FVRKAGGQRS 1150
    EDE 1153
    Length:1,153
    Mass (Da):129,086
    Last modified:August 1, 1998 - v1
    Checksum:i101B77D02B66B109
    GO
    Isoform Short (identifier: O61309-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         276-309: Missing.

    Show »
    Length:1,119
    Mass (Da):125,309
    Checksum:iF3882D2B9296497D
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei276 – 30934Missing in isoform Short. 1 PublicationVSP_003584Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF012531 mRNA. Translation: AAC17487.1.
    PIRiT31080.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF012531 mRNA. Translation: AAC17487.1 .
    PIRi T31080.

    3D structure databases

    ProteinModelPortali O61309.
    SMRi O61309. Positions 16-384.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 2 hits.
    3.90.340.10. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000333. NOS. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of NOS in a mollusc: expression in a giant modulatory neuron."
      Korneev S.A., Piper M.R., Picot J., Phillips R., Korneeva E.I., O'Shea M.
      J. Neurobiol. 35:65-76(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
      Tissue: CNS.

    Entry informationi

    Entry nameiNOS_LYMST
    AccessioniPrimary (citable) accession number: O61309
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3