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O61309

- NOS_LYMST

UniProt

O61309 - NOS_LYMST

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Protein

Nitric oxide synthase

Gene

NOS

Organism
Lymnaea stagnalis (Great pond snail) (Helix stagnalis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body.By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarity
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • FMNBy similarityNote: Binds 1 FMN.By similarity

Enzyme regulationi

Stimulated by calcium/calmodulin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi82 – 821Iron (heme axial ligand)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi556 – 58732FMNPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi697 – 70812FADBy similarityAdd
BLAST
Nucleotide bindingi836 – 84611FADBy similarityAdd
BLAST
Nucleotide bindingi911 – 92919NADPBy similarityAdd
BLAST
Nucleotide bindingi1089 – 110416NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. FMN binding Source: InterPro
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. NADP binding Source: InterPro
  6. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase (EC:1.14.13.39)
Alternative name(s):
NOS type I
Neuronal NOS
Short name:
N-NOS
Short name:
nNOS
Gene namesi
Name:NOS
OrganismiLymnaea stagnalis (Great pond snail) (Helix stagnalis)
Taxonomic identifieri6523 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaHeterobranchiaEuthyneuraPanpulmonataHygrophilaLymnaeoideaLymnaeidaeLymnaea

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11531153Nitric oxide synthasePRO_0000170949Add
BLAST

Expressioni

Tissue specificityi

Expressed in the central nervous system, in the serotonergic cerebral giant cells. The isoform Long and isoform Short are expressed equally in the CNS.

Structurei

3D structure databases

ProteinModelPortaliO61309.
SMRiO61309. Positions 16-384.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini427 – 610184Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini660 – 903244FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST
Repeati934 – 94071
Repeati941 – 94772
Repeati948 – 95473
Repeati955 – 96174
Repeati962 – 96875
Repeati969 – 97576
Repeati976 – 98277
Repeati983 – 98978
Repeati990 – 99679
Repeati997 – 1003710
Repeati1004 – 1010711

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni397 – 41721Calmodulin-bindingSequence AnalysisAdd
BLAST
Regioni934 – 10107711 X 7 AA tandem repeats of E-[NTR]-[ST]-[IM]-[PLQ]-[SP]-[CW]Add
BLAST

Sequence similaritiesi

Belongs to the NOS family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: O61309-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSLSQQAHG PPDAPRSKEE LLIHAKDFIN QYFTSFQMNK TRAHFHRLGE
60 70 80 90 100
INDLIEKSGT YDLTMAELTF GAKHAWRNAP GCIGRSQWSK LQVFDAREIG
110 120 130 140 150
TPREMFEALC SHIRYATNEG KIRSTITIFP QRKEGRPDFR VWNTQLISYA
160 170 180 190 200
GYKLGDGKVI GDPANVEFTE MCVEMGWKPK HGMFDLLPLV LSAAENSPEY
210 220 230 240 250
FELPTELVLE VTLKHPEYPW FAEMGLKWYA LPTDSGMLLD CGGLEFPSCP
260 270 280 290 300
FNGWFMGTMI GSRNLCDPHR YNMLEPIGLK MGLNTETASS LWKDRVLIEV
310 320 330 340 350
NVAVLYSFES ANVTIVNHHD ASTDFISHMD KEIKLRGGCP SDWVRMVPPM
360 370 380 390 400
SGSTLEVFHQ EMLLYNLHPA FVRQDVKPWK KHVWKSDQSV PINSCNPKRK
410 420 430 440 450
LGFKALARAV EFSASLMSKA LSSRVKCSIF YATETGRSER FARRLSEIFK
460 470 480 490 500
PVFHSRVVCM DDYAVETLEH ESLVMVITST FGNGEPPENG KQFAQSLLDM
510 520 530 540 550
KRKYDCDLGF LESCSSISTC IKSSILTEGP LAADVIGDRQ SLAMGTGPLC
560 570 580 590 600
NVRFAVFGLG SKAYPYYAAY GKYIYLMLQE LGAERLVNYC AGDALYGQEQ
610 620 630 640 650
SFRAWSEEVF KASCEAFCLD NRNDAPGPQT KGDCSKVRIV PVENCQEPDL
660 670 680 690 700
CQVLRNIHGK EVMPLILAER IQLQAKDSDQ QTILIKLDAH NATDLKYAPG
710 720 730 740 750
DHVAIFPANS PEIVDAILVR LDTSKGPSPD QVVKTEISTQ LGTNDTWRSH
760 770 780 790 800
LPICTSRTAF SFLLDVTTPP SQEILQVLAT QASSDMDKHK LEQLASNSEA
810 820 830 840 850
YEKWRLDLSP NILEILDEFP SLKIPPSLLL TQLPLLQPRY YSISSSQQKN
860 870 880 890 900
PNEVHATIAV VRFKTQDGDG PVHEGVCSSW LNRSPIGTVV PCFLRSAPHF
910 920 930 940 950
HLPEDPSLPI IMIGPGSGIA PFRSFWQQRL GEIENTMPSC ENTMLSCETT
960 970 980 990 1000
IPSCENSMPS CENTMPSCEN TMPSCENTIP SCENTIPSCE NTMPSCENTI
1010 1020 1030 1040 1050
PSWERTMQPC QIILPSQTKK HFGEMVLYTG CRTAKHMIYA AELEEMKRLG
1060 1070 1080 1090 1100
VLSNYHVALS REAALPKMYV QDIIIKNAAA VYEIVMKKGG HFYVSGDVSM
1110 1120 1130 1140 1150
AHDVTRALEL VLCQQGGREA SQQVMSLRDE NLFHEDIFGS FVRKAGGQRS

EDE
Length:1,153
Mass (Da):129,086
Last modified:August 1, 1998 - v1
Checksum:i101B77D02B66B109
GO
Isoform Short (identifier: O61309-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     276-309: Missing.

Show »
Length:1,119
Mass (Da):125,309
Checksum:iF3882D2B9296497D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei276 – 30934Missing in isoform Short. 1 PublicationVSP_003584Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012531 mRNA. Translation: AAC17487.1.
PIRiT31080.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012531 mRNA. Translation: AAC17487.1 .
PIRi T31080.

3D structure databases

ProteinModelPortali O61309.
SMRi O61309. Positions 16-384.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000333. NOS. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of NOS in a mollusc: expression in a giant modulatory neuron."
    Korneev S.A., Piper M.R., Picot J., Phillips R., Korneeva E.I., O'Shea M.
    J. Neurobiol. 35:65-76(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    Tissue: CNS.

Entry informationi

Entry nameiNOS_LYMST
AccessioniPrimary (citable) accession number: O61309
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 26, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3