Reviewed, UniProtKB/Swiss-Prot O61309 (NOS_LYMST)

Names and origin

Protein names

Recommended name:
    Nitric oxide synthase
    EC=1.14.13.39
Alternative name(s):
    Neuronal NOS
      Short name=N-NOS
      Short name=nNOS
    NOS type I

Gene names

Name:

NOS

Organism

Lymnaea stagnalis (Great pond snail)

Taxonomic identifier

6523 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Mollusca › Gastropoda › Pulmonata › Basommatophora › Lymnaeoidea › Lymnaeidae › Lymnaea

Protein attributes

Sequence length	1153 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body By similarity.
Catalytic activity	L-arginine + n NADPH + m O(2) = citrulline + nitric oxide + n NADP(+).
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity.
Enzyme regulation	Stimulated by calcium/calmodulin.
Tissue specificity	Expressed in the central nervous system, in the serotonergic cerebral giant cells. The isoform Long and isoform Short are expressed equally in the CNS.
Sequence similarities	Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro FMN binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro calmodulin binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro nitric-oxide synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: O61309-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: O61309-2)

The sequence of this isoform differs from the canonical sequence as follows:
     276-309: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1153	1153	Nitric oxide synthase		PRO_0000170949
Regions
	Domain	427 – 610	184	Flavodoxin-like
	Domain	660 – 903	244	FAD-binding FR-type
	Repeat	934 – 940	7	1
	Repeat	941 – 947	7	2
	Repeat	948 – 954	7	3
	Repeat	955 – 961	7	4
	Repeat	962 – 968	7	5
	Repeat	969 – 975	7	6
	Repeat	976 – 982	7	7
	Repeat	983 – 989	7	8
	Repeat	990 – 996	7	9
	Repeat	997 – 1003	7	10
	Repeat	1004 – 1010	7	11
	Nucleotide binding	556 – 587	32	FMN By similarity
	Nucleotide binding	697 – 708	12	FAD By similarity
	Nucleotide binding	836 – 846	11	FAD By similarity
	Nucleotide binding	911 – 929	19	NADP By similarity
	Nucleotide binding	1089 – 1104	16	NADP By similarity
	Region	397 – 417	21	Calmodulin-binding Potential
	Region	934 – 1010	77	11 X 7 AA tandem repeats of E-[NTR]-[ST]-[IM]-[PLQ]-[SP]-[CW]
Sites
	Metal binding	82	1	Iron (heme axial ligand) By similarity
Natural variations
	Alternative sequence	276 – 309	34	Missing in isoform Short.		VSP_003584

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 101B77D02B66B109	FASTA	1,153	129,086
	10 20 30 40 50 60 MGSLSQQAHG PPDAPRSKEE LLIHAKDFIN QYFTSFQMNK TRAHFHRLGE INDLIEKSGT 70 80 90 100 110 120 YDLTMAELTF GAKHAWRNAP GCIGRSQWSK LQVFDAREIG TPREMFEALC SHIRYATNEG 130 140 150 160 170 180 KIRSTITIFP QRKEGRPDFR VWNTQLISYA GYKLGDGKVI GDPANVEFTE MCVEMGWKPK 190 200 210 220 230 240 HGMFDLLPLV LSAAENSPEY FELPTELVLE VTLKHPEYPW FAEMGLKWYA LPTDSGMLLD 250 260 270 280 290 300 CGGLEFPSCP FNGWFMGTMI GSRNLCDPHR YNMLEPIGLK MGLNTETASS LWKDRVLIEV 310 320 330 340 350 360 NVAVLYSFES ANVTIVNHHD ASTDFISHMD KEIKLRGGCP SDWVRMVPPM SGSTLEVFHQ 370 380 390 400 410 420 EMLLYNLHPA FVRQDVKPWK KHVWKSDQSV PINSCNPKRK LGFKALARAV EFSASLMSKA 430 440 450 460 470 480 LSSRVKCSIF YATETGRSER FARRLSEIFK PVFHSRVVCM DDYAVETLEH ESLVMVITST 490 500 510 520 530 540 FGNGEPPENG KQFAQSLLDM KRKYDCDLGF LESCSSISTC IKSSILTEGP LAADVIGDRQ 550 560 570 580 590 600 SLAMGTGPLC NVRFAVFGLG SKAYPYYAAY GKYIYLMLQE LGAERLVNYC AGDALYGQEQ 610 620 630 640 650 660 SFRAWSEEVF KASCEAFCLD NRNDAPGPQT KGDCSKVRIV PVENCQEPDL CQVLRNIHGK 670 680 690 700 710 720 EVMPLILAER IQLQAKDSDQ QTILIKLDAH NATDLKYAPG DHVAIFPANS PEIVDAILVR 730 740 750 760 770 780 LDTSKGPSPD QVVKTEISTQ LGTNDTWRSH LPICTSRTAF SFLLDVTTPP SQEILQVLAT 790 800 810 820 830 840 QASSDMDKHK LEQLASNSEA YEKWRLDLSP NILEILDEFP SLKIPPSLLL TQLPLLQPRY 850 860 870 880 890 900 YSISSSQQKN PNEVHATIAV VRFKTQDGDG PVHEGVCSSW LNRSPIGTVV PCFLRSAPHF 910 920 930 940 950 960 HLPEDPSLPI IMIGPGSGIA PFRSFWQQRL GEIENTMPSC ENTMLSCETT IPSCENSMPS 970 980 990 1000 1010 1020 CENTMPSCEN TMPSCENTIP SCENTIPSCE NTMPSCENTI PSWERTMQPC QIILPSQTKK 1030 1040 1050 1060 1070 1080 HFGEMVLYTG CRTAKHMIYA AELEEMKRLG VLSNYHVALS REAALPKMYV QDIIIKNAAA 1090 1100 1110 1120 1130 1140 VYEIVMKKGG HFYVSGDVSM AHDVTRALEL VLCQQGGREA SQQVMSLRDE NLFHEDIFGS 1150 FVRKAGGQRS EDE
	Isoform Short [UniParc]. Checksum: F3882D2B9296497D		1,119	125,309
	10 20 30 40 50 60 MGSLSQQAHG PPDAPRSKEE LLIHAKDFIN QYFTSFQMNK TRAHFHRLGE INDLIEKSGT 70 80 90 100 110 120 YDLTMAELTF GAKHAWRNAP GCIGRSQWSK LQVFDAREIG TPREMFEALC SHIRYATNEG 130 140 150 160 170 180 KIRSTITIFP QRKEGRPDFR VWNTQLISYA GYKLGDGKVI GDPANVEFTE MCVEMGWKPK 190 200 210 220 230 240 HGMFDLLPLV LSAAENSPEY FELPTELVLE VTLKHPEYPW FAEMGLKWYA LPTDSGMLLD 250 260 270 280 290 300 CGGLEFPSCP FNGWFMGTMI GSRNLCDPHR YNMLESANVT IVNHHDASTD FISHMDKEIK 310 320 330 340 350 360 LRGGCPSDWV RMVPPMSGST LEVFHQEMLL YNLHPAFVRQ DVKPWKKHVW KSDQSVPINS 370 380 390 400 410 420 CNPKRKLGFK ALARAVEFSA SLMSKALSSR VKCSIFYATE TGRSERFARR LSEIFKPVFH 430 440 450 460 470 480 SRVVCMDDYA VETLEHESLV MVITSTFGNG EPPENGKQFA QSLLDMKRKY DCDLGFLESC 490 500 510 520 530 540 SSISTCIKSS ILTEGPLAAD VIGDRQSLAM GTGPLCNVRF AVFGLGSKAY PYYAAYGKYI 550 560 570 580 590 600 YLMLQELGAE RLVNYCAGDA LYGQEQSFRA WSEEVFKASC EAFCLDNRND APGPQTKGDC 610 620 630 640 650 660 SKVRIVPVEN CQEPDLCQVL RNIHGKEVMP LILAERIQLQ AKDSDQQTIL IKLDAHNATD 670 680 690 700 710 720 LKYAPGDHVA IFPANSPEIV DAILVRLDTS KGPSPDQVVK TEISTQLGTN DTWRSHLPIC 730 740 750 760 770 780 TSRTAFSFLL DVTTPPSQEI LQVLATQASS DMDKHKLEQL ASNSEAYEKW RLDLSPNILE 790 800 810 820 830 840 ILDEFPSLKI PPSLLLTQLP LLQPRYYSIS SSQQKNPNEV HATIAVVRFK TQDGDGPVHE 850 860 870 880 890 900 GVCSSWLNRS PIGTVVPCFL RSAPHFHLPE DPSLPIIMIG PGSGIAPFRS FWQQRLGEIE 910 920 930 940 950 960 NTMPSCENTM LSCETTIPSC ENSMPSCENT MPSCENTMPS CENTIPSCEN TIPSCENTMP 970 980 990 1000 1010 1020 SCENTIPSWE RTMQPCQIIL PSQTKKHFGE MVLYTGCRTA KHMIYAAELE EMKRLGVLSN 1030 1040 1050 1060 1070 1080 YHVALSREAA LPKMYVQDII IKNAAAVYEI VMKKGGHFYV SGDVSMAHDV TRALELVLCQ 1090 1100 1110 QGGREASQQV MSLRDENLFH EDIFGSFVRK AGGQRSEDE

References

[1]

"Molecular characterization of NOS in a mollusc: expression in a giant modulatory neuron." Korneev S.A., Piper M.R., Picot J., Phillips R., Korneeva E.I., O'Shea M. J. Neurobiol. 35:65-76(1998) [PubMed: 9552167] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT). Tissue: CNS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF012531 mRNA. Translation: AAC17487.1.
PIR	T31080.
3D structure databases
HSSP	HSSP built from PDB template 1DD7 based on UniProtKB P29477.
ModBase	Search...
Family and domain databases
InterPro	IPR003097. FAD-binding_1. IPR001094. Flavdoxin_like. IPR008254. Flavodoxin/NO_synth. IPR001709. FPN_cyt_redctse. IPR004030. NO_synthase_oxygenase_reg. IPR012144. NOS. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Gene3D	G3DSA:3.90.340.10. NO_synthase_oxygenase_reg. 1 hit.
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 2 hits. PF02898. NO_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000333. NOS. 1 hit.
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. PS60001. NOS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOS_LYMST

Accession

Primary (citable) accession number: O61309

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 25, 2008
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)