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Protein

Nitric oxide synthase

Gene

NOS

Organism
Lymnaea stagnalis (Great pond snail) (Helix stagnalis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body.By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarity
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • FMNBy similarityNote: Binds 1 FMN.By similarity

Enzyme regulationi

Stimulated by calcium/calmodulin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi82Iron (heme axial ligand)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi556 – 587FMNPROSITE-ProRule annotationAdd BLAST32
Nucleotide bindingi697 – 708FADBy similarityAdd BLAST12
Nucleotide bindingi836 – 846FADBy similarityAdd BLAST11
Nucleotide bindingi911 – 929NADPBy similarityAdd BLAST19
Nucleotide bindingi1089 – 1104NADPBy similarityAdd BLAST16

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase (EC:1.14.13.39)
Alternative name(s):
NOS type I
Neuronal NOS
Short name:
N-NOS
Short name:
nNOS
Gene namesi
Name:NOS
OrganismiLymnaea stagnalis (Great pond snail) (Helix stagnalis)
Taxonomic identifieri6523 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaHeterobranchiaEuthyneuraPanpulmonataHygrophilaLymnaeoideaLymnaeidaeLymnaea

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001709491 – 1153Nitric oxide synthaseAdd BLAST1153

Expressioni

Tissue specificityi

Expressed in the central nervous system, in the serotonergic cerebral giant cells. The isoform Long and isoform Short are expressed equally in the CNS.

Structurei

3D structure databases

ProteinModelPortaliO61309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini427 – 610Flavodoxin-likePROSITE-ProRule annotationAdd BLAST184
Domaini660 – 903FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST244
Repeati934 – 94017
Repeati941 – 94727
Repeati948 – 95437
Repeati955 – 96147
Repeati962 – 96857
Repeati969 – 97567
Repeati976 – 98277
Repeati983 – 98987
Repeati990 – 99697
Repeati997 – 1003107
Repeati1004 – 1010117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni397 – 417Calmodulin-bindingSequence analysisAdd BLAST21
Regioni934 – 101011 X 7 AA tandem repeats of E-[NTR]-[ST]-[IM]-[PLQ]-[SP]-[CW]Add BLAST77

Sequence similaritiesi

Belongs to the NOS family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: O61309-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSLSQQAHG PPDAPRSKEE LLIHAKDFIN QYFTSFQMNK TRAHFHRLGE
60 70 80 90 100
INDLIEKSGT YDLTMAELTF GAKHAWRNAP GCIGRSQWSK LQVFDAREIG
110 120 130 140 150
TPREMFEALC SHIRYATNEG KIRSTITIFP QRKEGRPDFR VWNTQLISYA
160 170 180 190 200
GYKLGDGKVI GDPANVEFTE MCVEMGWKPK HGMFDLLPLV LSAAENSPEY
210 220 230 240 250
FELPTELVLE VTLKHPEYPW FAEMGLKWYA LPTDSGMLLD CGGLEFPSCP
260 270 280 290 300
FNGWFMGTMI GSRNLCDPHR YNMLEPIGLK MGLNTETASS LWKDRVLIEV
310 320 330 340 350
NVAVLYSFES ANVTIVNHHD ASTDFISHMD KEIKLRGGCP SDWVRMVPPM
360 370 380 390 400
SGSTLEVFHQ EMLLYNLHPA FVRQDVKPWK KHVWKSDQSV PINSCNPKRK
410 420 430 440 450
LGFKALARAV EFSASLMSKA LSSRVKCSIF YATETGRSER FARRLSEIFK
460 470 480 490 500
PVFHSRVVCM DDYAVETLEH ESLVMVITST FGNGEPPENG KQFAQSLLDM
510 520 530 540 550
KRKYDCDLGF LESCSSISTC IKSSILTEGP LAADVIGDRQ SLAMGTGPLC
560 570 580 590 600
NVRFAVFGLG SKAYPYYAAY GKYIYLMLQE LGAERLVNYC AGDALYGQEQ
610 620 630 640 650
SFRAWSEEVF KASCEAFCLD NRNDAPGPQT KGDCSKVRIV PVENCQEPDL
660 670 680 690 700
CQVLRNIHGK EVMPLILAER IQLQAKDSDQ QTILIKLDAH NATDLKYAPG
710 720 730 740 750
DHVAIFPANS PEIVDAILVR LDTSKGPSPD QVVKTEISTQ LGTNDTWRSH
760 770 780 790 800
LPICTSRTAF SFLLDVTTPP SQEILQVLAT QASSDMDKHK LEQLASNSEA
810 820 830 840 850
YEKWRLDLSP NILEILDEFP SLKIPPSLLL TQLPLLQPRY YSISSSQQKN
860 870 880 890 900
PNEVHATIAV VRFKTQDGDG PVHEGVCSSW LNRSPIGTVV PCFLRSAPHF
910 920 930 940 950
HLPEDPSLPI IMIGPGSGIA PFRSFWQQRL GEIENTMPSC ENTMLSCETT
960 970 980 990 1000
IPSCENSMPS CENTMPSCEN TMPSCENTIP SCENTIPSCE NTMPSCENTI
1010 1020 1030 1040 1050
PSWERTMQPC QIILPSQTKK HFGEMVLYTG CRTAKHMIYA AELEEMKRLG
1060 1070 1080 1090 1100
VLSNYHVALS REAALPKMYV QDIIIKNAAA VYEIVMKKGG HFYVSGDVSM
1110 1120 1130 1140 1150
AHDVTRALEL VLCQQGGREA SQQVMSLRDE NLFHEDIFGS FVRKAGGQRS

EDE
Length:1,153
Mass (Da):129,086
Last modified:August 1, 1998 - v1
Checksum:i101B77D02B66B109
GO
Isoform Short (identifier: O61309-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     276-309: Missing.

Show »
Length:1,119
Mass (Da):125,309
Checksum:iF3882D2B9296497D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003584276 – 309Missing in isoform Short. 1 PublicationAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012531 mRNA. Translation: AAC17487.1.
PIRiT31080.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012531 mRNA. Translation: AAC17487.1.
PIRiT31080.

3D structure databases

ProteinModelPortaliO61309.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNOS_LYMST
AccessioniPrimary (citable) accession number: O61309
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: October 5, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.