O61309 (NOS_LYMST) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 95.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Nitric oxide synthase EC=1.14.13.39 Alternative name(s): NOS type I Neuronal NOS Short name=N-NOS Short name=nNOS | ||
| Gene names |
| ||
| Organism | Lymnaea stagnalis (Great pond snail) | ||
| Taxonomic identifier | 6523 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Lophotrochozoa › Mollusca › Gastropoda › Heterobranchia › Euthyneura › Panpulmonata › Hygrophila › Lymnaeoidea › Lymnaeidae › Lymnaea |
Protein attributes
| Sequence length | 1153 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body By similarity. |
| Catalytic activity | 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. |
| Cofactor | Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. |
| Enzyme regulation | Stimulated by calcium/calmodulin. |
| Tissue specificity | Expressed in the central nervous system, in the serotonergic cerebral giant cells. The isoform Long and isoform Short are expressed equally in the CNS. |
| Sequence similarities | Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing |
| Domain | Repeat |
| Ligand | Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP |
| Molecular function | Oxidoreductase |
| Gene Ontology (GO) | |
| Biological_process | nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro |
| Molecular_function | FMN binding Inferred from electronic annotation. Source: InterPro NADP bindingInferred from electronic annotation. Source: InterPro flavin adenine dinucleotide bindingInferred from electronic annotation. Source: InterPro heme bindingInferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: InterPro nitric-oxide synthase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform Long (identifier: O61309-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Short (identifier: O61309-2) The sequence of this isoform differs from the canonical sequence as follows: 276-309: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1153 | 1153 | Nitric oxide synthase | PRO_0000170949 | |||||
Regions | |||||||||
| Domain | 427 – 610 | 184 | Flavodoxin-like | ||||||
| Domain | 660 – 903 | 244 | FAD-binding FR-type | ||||||
| Repeat | 934 – 940 | 7 | 1 | ||||||
| Repeat | 941 – 947 | 7 | 2 | ||||||
| Repeat | 948 – 954 | 7 | 3 | ||||||
| Repeat | 955 – 961 | 7 | 4 | ||||||
| Repeat | 962 – 968 | 7 | 5 | ||||||
| Repeat | 969 – 975 | 7 | 6 | ||||||
| Repeat | 976 – 982 | 7 | 7 | ||||||
| Repeat | 983 – 989 | 7 | 8 | ||||||
| Repeat | 990 – 996 | 7 | 9 | ||||||
| Repeat | 997 – 1003 | 7 | 10 | ||||||
| Repeat | 1004 – 1010 | 7 | 11 | ||||||
| Nucleotide binding | 556 – 587 | 32 | FMN By similarity | ||||||
| Nucleotide binding | 697 – 708 | 12 | FAD By similarity | ||||||
| Nucleotide binding | 836 – 846 | 11 | FAD By similarity | ||||||
| Nucleotide binding | 911 – 929 | 19 | NADP By similarity | ||||||
| Nucleotide binding | 1089 – 1104 | 16 | NADP By similarity | ||||||
| Region | 397 – 417 | 21 | Calmodulin-binding Potential | ||||||
| Region | 934 – 1010 | 77 | 11 X 7 AA tandem repeats of E-[NTR]-[ST]-[IM]-[PLQ]-[SP]-[CW] | ||||||
Sites | |||||||||
| Metal binding | 82 | 1 | Iron (heme axial ligand) By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 276 – 309 | 34 | Missing in isoform Short. | VSP_003584 | |||||
Sequences
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References
| [1] | "Molecular characterization of NOS in a mollusc: expression in a giant modulatory neuron." Korneev S.A., Piper M.R., Picot J., Phillips R., Korneeva E.I., O'Shea M. J. Neurobiol. 35:65-76(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT). Tissue: CNS. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF012531 mRNA. Translation: AAC17487.1. |
| PIR | T31080. |
3D structure databases | |
| ProteinModelPortal | O61309. |
| SMR | O61309. Positions 16-384. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Family and domain databases | |
| Gene3D | 1.20.990.10. 1 hit. 3.90.340.10. 1 hit. |
| InterPro | IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001094. Flavdoxin. IPR008254. Flavodoxin/NO_synth. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR023173. NADPH_Cyt_P450_Rdtase_dom3. IPR004030. NO_synthase_oxygenase_dom. IPR026009. NOS. IPR012144. NOS_met. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view] |
| PANTHER | PTHR19384:SF5. PTHR19384:SF5. 1 hit. |
| Pfam | PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. PF02898. NO_synthase. 1 hit. [Graphical view] |
| PIRSF | PIRSF000333. NOS. 1 hit. |
| PRINTS | PR00369. FLAVODOXIN. PR00371. FPNCR. |
| SUPFAM | SSF56512. NO_synthase_oxygenase_reg. 1 hit. SSF63380. Riboflavin_synthase_like_b-brl. 1 hit. |
| PROSITE | PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. PS60001. NOS. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NOS_LYMST | ||||||||
| Accession | Primary (citable) accession number: O61309 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |