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O61309 (NOS_LYMST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitric oxide synthase

EC=1.14.13.39
Alternative name(s):
NOS type I
Neuronal NOS
Short name=N-NOS
Short name=nNOS
Gene names
Name:NOS
OrganismLymnaea stagnalis (Great pond snail) (Helix stagnalis)
Taxonomic identifier6523 [NCBI]
Taxonomic lineageEukaryotaMetazoaLophotrochozoaMolluscaGastropodaHeterobranchiaEuthyneuraPanpulmonataHygrophilaLymnaeoideaLymnaeidaeLymnaea

Protein attributes

Sequence length1153 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body By similarity.

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Enzyme regulation

Stimulated by calcium/calmodulin.

Tissue specificity

Expressed in the central nervous system, in the serotonergic cerebral giant cells. The isoform Long and isoform Short are expressed equally in the CNS.

Sequence similarities

Belongs to the NOS family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: O61309-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: O61309-2)

The sequence of this isoform differs from the canonical sequence as follows:
     276-309: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11531153Nitric oxide synthase
PRO_0000170949

Regions

Domain427 – 610184Flavodoxin-like
Domain660 – 903244FAD-binding FR-type
Repeat934 – 94071
Repeat941 – 94772
Repeat948 – 95473
Repeat955 – 96174
Repeat962 – 96875
Repeat969 – 97576
Repeat976 – 98277
Repeat983 – 98978
Repeat990 – 99679
Repeat997 – 1003710
Repeat1004 – 1010711
Nucleotide binding556 – 58732FMN By similarity
Nucleotide binding697 – 70812FAD By similarity
Nucleotide binding836 – 84611FAD By similarity
Nucleotide binding911 – 92919NADP By similarity
Nucleotide binding1089 – 110416NADP By similarity
Region397 – 41721Calmodulin-binding Potential
Region934 – 10107711 X 7 AA tandem repeats of E-[NTR]-[ST]-[IM]-[PLQ]-[SP]-[CW]

Sites

Metal binding821Iron (heme axial ligand) By similarity

Natural variations

Alternative sequence276 – 30934Missing in isoform Short.
VSP_003584

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 101B77D02B66B109

FASTA1,153129,086
        10         20         30         40         50         60 
MGSLSQQAHG PPDAPRSKEE LLIHAKDFIN QYFTSFQMNK TRAHFHRLGE INDLIEKSGT 

        70         80         90        100        110        120 
YDLTMAELTF GAKHAWRNAP GCIGRSQWSK LQVFDAREIG TPREMFEALC SHIRYATNEG 

       130        140        150        160        170        180 
KIRSTITIFP QRKEGRPDFR VWNTQLISYA GYKLGDGKVI GDPANVEFTE MCVEMGWKPK 

       190        200        210        220        230        240 
HGMFDLLPLV LSAAENSPEY FELPTELVLE VTLKHPEYPW FAEMGLKWYA LPTDSGMLLD 

       250        260        270        280        290        300 
CGGLEFPSCP FNGWFMGTMI GSRNLCDPHR YNMLEPIGLK MGLNTETASS LWKDRVLIEV 

       310        320        330        340        350        360 
NVAVLYSFES ANVTIVNHHD ASTDFISHMD KEIKLRGGCP SDWVRMVPPM SGSTLEVFHQ 

       370        380        390        400        410        420 
EMLLYNLHPA FVRQDVKPWK KHVWKSDQSV PINSCNPKRK LGFKALARAV EFSASLMSKA 

       430        440        450        460        470        480 
LSSRVKCSIF YATETGRSER FARRLSEIFK PVFHSRVVCM DDYAVETLEH ESLVMVITST 

       490        500        510        520        530        540 
FGNGEPPENG KQFAQSLLDM KRKYDCDLGF LESCSSISTC IKSSILTEGP LAADVIGDRQ 

       550        560        570        580        590        600 
SLAMGTGPLC NVRFAVFGLG SKAYPYYAAY GKYIYLMLQE LGAERLVNYC AGDALYGQEQ 

       610        620        630        640        650        660 
SFRAWSEEVF KASCEAFCLD NRNDAPGPQT KGDCSKVRIV PVENCQEPDL CQVLRNIHGK 

       670        680        690        700        710        720 
EVMPLILAER IQLQAKDSDQ QTILIKLDAH NATDLKYAPG DHVAIFPANS PEIVDAILVR 

       730        740        750        760        770        780 
LDTSKGPSPD QVVKTEISTQ LGTNDTWRSH LPICTSRTAF SFLLDVTTPP SQEILQVLAT 

       790        800        810        820        830        840 
QASSDMDKHK LEQLASNSEA YEKWRLDLSP NILEILDEFP SLKIPPSLLL TQLPLLQPRY 

       850        860        870        880        890        900 
YSISSSQQKN PNEVHATIAV VRFKTQDGDG PVHEGVCSSW LNRSPIGTVV PCFLRSAPHF 

       910        920        930        940        950        960 
HLPEDPSLPI IMIGPGSGIA PFRSFWQQRL GEIENTMPSC ENTMLSCETT IPSCENSMPS 

       970        980        990       1000       1010       1020 
CENTMPSCEN TMPSCENTIP SCENTIPSCE NTMPSCENTI PSWERTMQPC QIILPSQTKK 

      1030       1040       1050       1060       1070       1080 
HFGEMVLYTG CRTAKHMIYA AELEEMKRLG VLSNYHVALS REAALPKMYV QDIIIKNAAA 

      1090       1100       1110       1120       1130       1140 
VYEIVMKKGG HFYVSGDVSM AHDVTRALEL VLCQQGGREA SQQVMSLRDE NLFHEDIFGS 

      1150 
FVRKAGGQRS EDE 

« Hide

Isoform Short [UniParc].

Checksum: F3882D2B9296497D
Show »

FASTA1,119125,309

References

[1]"Molecular characterization of NOS in a mollusc: expression in a giant modulatory neuron."
Korneev S.A., Piper M.R., Picot J., Phillips R., Korneeva E.I., O'Shea M.
J. Neurobiol. 35:65-76(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
Tissue: CNS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF012531 mRNA. Translation: AAC17487.1.
PIRT31080.

3D structure databases

ProteinModelPortalO61309.
SMRO61309. Positions 16-384.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.20.990.10. 1 hit.
3.40.50.360. 2 hits.
3.90.340.10. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR004030. NO_synthase_oxygenase_dom.
IPR012144. NOS_euk.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF000333. NOS. 1 hit.
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNOS_LYMST
AccessionPrimary (citable) accession number: O61309
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: June 11, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families