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Protein

Ovarian-specific serine/threonine-protein kinase Lok

Gene

lok

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May have a role in germline establishment.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei203 – 2031ATPPROSITE-ProRule annotation
Active sitei303 – 3031Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi180 – 1889ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: BHF-UCL
  • protein serine/threonine kinase activity Source: UniProtKB
  • tau-protein kinase activity Source: FlyBase

GO - Biological processi

  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: FlyBase
  • apoptotic process Source: FlyBase
  • cellular response to DNA damage stimulus Source: FlyBase
  • cellular response to gamma radiation Source: BHF-UCL
  • DNA damage checkpoint Source: FlyBase
  • ectopic germ cell programmed cell death Source: FlyBase
  • germ cell development Source: UniProtKB
  • intrinsic apoptotic signaling pathway by p53 class mediator Source: BHF-UCL
  • intrinsic apoptotic signaling pathway in response to DNA damage Source: BHF-UCL
  • mitotic DNA damage checkpoint Source: FlyBase
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • protein phosphorylation Source: UniProtKB
  • regulation of DNA repair Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 1994.
ReactomeiR-DME-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-DME-69473. G2/M DNA damage checkpoint.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
SignaLinkiO61267.

Names & Taxonomyi

Protein namesi
Recommended name:
Ovarian-specific serine/threonine-protein kinase Lok (EC:2.7.11.1)
Alternative name(s):
Protein loki
dMNK
Gene namesi
Name:lok
ORF Names:CG10895
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0019686. lok.

Subcellular locationi

  • Nucleus speckle 1 Publication

  • Note: Speckled subnuclear compartment.

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • nuclear speck Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 476476Ovarian-specific serine/threonine-protein kinase LokPRO_0000086237Add
BLAST

Proteomic databases

PaxDbiO61267.
PRIDEiO61267.

PTM databases

iPTMnetiO61267.

Expressioni

Tissue specificityi

In stage 3 embryos, both isoforms are expressed in both somatic and pole cell nuclei. Expression in pole cell nuclei is sustained until stage 9 and weakly expressed after pole cell invagination into the abdominal cavity.1 Publication

Developmental stagei

Expressed both maternally and zygotically in adult females. Levels of the long isoform remain fairly constant from ovaries to embryos, the levels of short isoform decrease dramatically.1 Publication

Gene expression databases

BgeeiFBgn0019686.
ExpressionAtlasiO61267. differential.
GenevisibleiO61267. DM.

Interactioni

Protein-protein interaction databases

BioGridi61259. 12 interactions.
IntActiO61267. 6 interactions.
MINTiMINT-4080900.
STRINGi7227.FBpp0080860.

Structurei

3D structure databases

ProteinModelPortaliO61267.
SMRiO61267. Positions 95-476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini69 – 12961FHAPROSITE-ProRule annotationCuratedAdd
BLAST
Domaini174 – 441268Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FHA domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0615. Eukaryota.
ENOG410YA63. LUCA.
GeneTreeiENSGT00800000124190.
InParanoidiO61267.
KOiK06641.
OMAiYYINRKL.
OrthoDBiEOG091G0DVW.
PhylomeDBiO61267.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR000253. FHA_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 2 hits.
PfamiPF00498. FHA. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: O61267-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARDTQGTQG TQSQASNIWT QVESQPMEKI VWGRLYGKNI KIKSLGTSSK
60 70 80 90 100
YRIIYTHSSF SVDLNNDEFT AGRGEANDLI LTLNDLPEKI LTRISKVHFI
110 120 130 140 150
IKRANCELTN PVYIQDLSRN GTFVNNEKIG TNRMRILKND DVISLSHPTY
160 170 180 190 200
KAFVFKDLSP NESIGLPEEI NKTYYVNRKL GSGAYGLVRL VYDTRTCQQF
210 220 230 240 250
AMKIVKKNML SGARPSTNFS DPDRVLNEAK IMKNLSHPCV VRMHDIVDKP
260 270 280 290 300
DSVYMVLEFM RGGDLLNRII SNKLLSEDIS KLYFYQMCHA VKYLHDRGIT
310 320 330 340 350
HRDLKPDNVL LETNDEETLL KVSDFGLSKF VQKDSVMRTL CGTPLYVAPE
360 370 380 390 400
VLITGGREAY TKKVDIWSLG VVLFTCLSGT LPFSDEYGTP AAQQIKKGRF
410 420 430 440 450
AYGHPSWKSV SQRAKLLINQ MLIVDPERRP SIDDVLQSSW LRDAPMLQKA
460 470
KRLMKLDGME IEEENFLEPP TKRSRR
Length:476
Mass (Da):54,261
Last modified:August 1, 1998 - v1
Checksum:i58D583E015C4E626
GO
Isoform Short (identifier: O61267-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     46-62: Missing.

Show »
Length:459
Mass (Da):52,346
Checksum:i94AD72F8AF8491BD
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei46 – 6217Missing in isoform Short. 2 PublicationsVSP_004865Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007821 mRNA. Translation: BAA28755.1.
AB007822 mRNA. Translation: BAA28756.1.
U87984 mRNA. Translation: AAB49642.1.
AE014134 Genomic DNA. Translation: AAF53867.2.
AE014134 Genomic DNA. Translation: AAN11062.1.
AY070549 mRNA. Translation: AAL48020.1.
RefSeqiNP_477218.1. NM_057870.4. [O61267-2]
NP_477219.1. NM_057871.4. [O61267-1]
NP_724241.1. NM_165318.3. [O61267-2]
UniGeneiDm.475.

Genome annotation databases

EnsemblMetazoaiFBtr0081328; FBpp0080860; FBgn0019686. [O61267-1]
GeneIDi35288.
KEGGidme:Dmel_CG10895.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007821 mRNA. Translation: BAA28755.1.
AB007822 mRNA. Translation: BAA28756.1.
U87984 mRNA. Translation: AAB49642.1.
AE014134 Genomic DNA. Translation: AAF53867.2.
AE014134 Genomic DNA. Translation: AAN11062.1.
AY070549 mRNA. Translation: AAL48020.1.
RefSeqiNP_477218.1. NM_057870.4. [O61267-2]
NP_477219.1. NM_057871.4. [O61267-1]
NP_724241.1. NM_165318.3. [O61267-2]
UniGeneiDm.475.

3D structure databases

ProteinModelPortaliO61267.
SMRiO61267. Positions 95-476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61259. 12 interactions.
IntActiO61267. 6 interactions.
MINTiMINT-4080900.
STRINGi7227.FBpp0080860.

PTM databases

iPTMnetiO61267.

Proteomic databases

PaxDbiO61267.
PRIDEiO61267.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0081328; FBpp0080860; FBgn0019686. [O61267-1]
GeneIDi35288.
KEGGidme:Dmel_CG10895.

Organism-specific databases

CTDi35288.
FlyBaseiFBgn0019686. lok.

Phylogenomic databases

eggNOGiKOG0615. Eukaryota.
ENOG410YA63. LUCA.
GeneTreeiENSGT00800000124190.
InParanoidiO61267.
KOiK06641.
OMAiYYINRKL.
OrthoDBiEOG091G0DVW.
PhylomeDBiO61267.

Enzyme and pathway databases

BRENDAi2.7.11.1. 1994.
ReactomeiR-DME-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-DME-69473. G2/M DNA damage checkpoint.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
SignaLinkiO61267.

Miscellaneous databases

GenomeRNAii35288.
PROiO61267.

Gene expression databases

BgeeiFBgn0019686.
ExpressionAtlasiO61267. differential.
GenevisibleiO61267. DM.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR000253. FHA_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 2 hits.
PfamiPF00498. FHA. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLOK_DROME
AccessioniPrimary (citable) accession number: O61267
Secondary accession number(s): O61268, P91876, Q8SZS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.