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O61065

- RIR1_CRYPV

UniProt

O61065 - RIR1_CRYPV

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Protein

Ribonucleoside-diphosphate reductase large chain

Gene
RNR1
Organism
Cryptosporidium parvum
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the large subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521Allosteric activator By similarity
Binding sitei87 – 871Allosteric activator By similarity
Binding sitei200 – 2001Substrate By similarity
Sitei216 – 2161Important for hydrogen atom transfer By similarity
Sitei224 – 2241Allosteric effector binding, determines substrate specificity By similarity
Binding sitei245 – 2451Substrate; via amide nitrogen By similarity
Sitei254 – 2541Allosteric effector binding, determines substrate specificity By similarity
Active sitei425 – 4251Proton acceptor By similarity
Active sitei427 – 4271Cysteine radical intermediate By similarity
Active sitei429 – 4291Proton acceptor By similarity
Sitei442 – 4421Important for hydrogen atom transfer By similarity
Sitei737 – 7371Important for electron transfer By similarity
Sitei738 – 7381Important for electron transfer By similarity
Sitei798 – 7981Interacts with thioredoxin/glutaredoxin By similarity
Sitei801 – 8011Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB

GO - Biological processi

  1. deoxyribonucleotide biosynthetic process Source: UniProtKB
  2. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large chain (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase R1 subunit
Gene namesi
Name:RNR1
OrganismiCryptosporidium parvum
Taxonomic identifieri5807 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaConoidasidaCoccidiaEucoccidioridaEimeriorinaCryptosporidiidaeCryptosporidium

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 803803Ribonucleoside-diphosphate reductase large chainPRO_0000187196Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi216 ↔ 442Redox-active By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Structurei

3D structure databases

ProteinModelPortaliO61065.
SMRiO61065. Positions 15-789.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9191ATP-coneAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni11 – 177Allosteric activator binding By similarity
Regioni215 – 2162Substrate binding By similarity
Regioni283 – 2864Allosteric effector binding, determines substrate specificity By similarity
Regioni425 – 4295Substrate binding By similarity
Regioni603 – 6075Substrate binding By similarity

Sequence similaritiesi

Contains 1 ATP-cone domain.

Phylogenomic databases

eggNOGiCOG0209.

Family and domain databases

InterProiIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O61065-1 [UniParc]FASTAAdd to Basket

« Hide

MYVVNRKGEE EPVSFDQILS RITKLSYGLH PLVDPARVTQ AVINGLYSGI    50
KTSELDELAS QTCAYMAATH NDFSKLAARI STSNLHKNTS SDIGDVASQL 100
YNFKDNQGCP APLISKPVYD FIMENRERIN SKIDFSKDFE YDYFAFKTLE 150
RSYLLKIDNK VVERPQHLLM RVSCGIHCGD IEAALETYEL LSQKYFTHAT 200
PTLFNSGTPR PQMSSCFLLR IPEDSINGIF DTLTKCANIS KTAGGLGVAV 250
SNIRGTGSYI RGTNGRSNGL IPMLRVYNDT ARYIDQGGGK RKGAIAIYLE 300
PWHVDVVEFI EIRKNHGKEE MRCRDLFPAL WVPDLFMERV EKDQDWTLMC 350
PDECRGLQDV WGDDFKKLYE EYEKQGRGRK TMKAQKLWFL ILQAQIETGT 400
PFICYKDAAN SKSNQKNLGT IVSSNLCTEI IEYTSTDEVA VCNLASIGLP 450
KFVDKNNKTF DFDKLKEVTK VITRNLNKLI DVGYYSLKEC KKSNLRHRPL 500
GIGIQGLADC FMMLRMPYES EGAKKLNKQI FEVIYYAALD ASCELAEKYG 550
PYETYSGSPA SKGILQFDMW GVTPDSGLCD WDLLKDRISK HGIRNSLLIS 600
PMPTASTSQI LGNNESFEPF TSNIYHRRVL SGEFFVVNPH LLNDLLELGL 650
WDDRLKQNII ANNGSIQNIL TIPEDIRELY KTVWEIKQKT VIDMAADRGP 700
YVCQSQSLNI HMENANFAKL SSMHFYGWKK GLKTGIYYLR TQSATRPIQF 750
TVDQQLLKSE TKEKDSLETN KRQALEPEAQ KLIACPLRPT NMKDDEECMM 800
CSG 803
Length:803
Mass (Da):91,123
Last modified:August 14, 2001 - v2
Checksum:i9DBE7F2AC337F97F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF043243 Genomic DNA. Translation: AAC12280.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF043243 Genomic DNA. Translation: AAC12280.2 .

3D structure databases

ProteinModelPortali O61065.
SMRi O61065. Positions 15-789.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0209.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of ribonucleotide reductase from Cryptosporidium parvum."
    Akiyoshi D.E., Balakrishnan R., Huettinger C., Widmer G., Tzipori S.
    DNA Seq. 13:167-172(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: GCH1.

Entry informationi

Entry nameiRIR1_CRYPV
AccessioniPrimary (citable) accession number: O61065
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 14, 2001
Last modified: October 16, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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