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Protein

Cullin-1

Gene

culA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable core component of cullin-based SCF-like E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit (By similarity). Required at several stages during development. CulA and fbxA regulate multicellular development by targeting regA for degradation via a pathway that requires erkB function, leading to an increase in cAMP and PKA activity.By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ubiquitin-protein transferase activity Source: dictyBase

GO - Biological processi

  • cell differentiation Source: dictyBase
  • chemotaxis Source: dictyBase
  • G1/S transition of mitotic cell cycle Source: dictyBase
  • sorocarp development Source: dictyBase
  • ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-DDI-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DDI-69231. Cyclin D associated events in G1.
R-DDI-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-1
Short name:
CUL-1
Alternative name(s):
Cullin-A
Gene namesi
Name:culA
Synonyms:cul1
ORF Names:DDB_G0291972
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291972. culA.

Subcellular locationi

GO - Cellular componenti

  • SCF ubiquitin ligase complex Source: dictyBase
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells are defective in inducing cell-type-specific gene expression and exhibit defects during aggregation, including reduced chemotaxis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 770770Cullin-1PRO_0000345010Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki714 – 714Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)By similarity

Post-translational modificationi

Neddylated.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO60999.

Interactioni

Subunit structurei

Part of a complex that includes culA, fbxA and regA. Formation of this complex is dependent on the MAP kinase erkB.1 Publication

Protein-protein interaction databases

STRINGi44689.DDB0185191.

Structurei

3D structure databases

ProteinModelPortaliO60999.
SMRiO60999. Positions 15-770.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2166. Eukaryota.
COG5647. LUCA.
InParanoidiO60999.
KOiK03347.
OMAiHIANQGL.
PhylomeDBiO60999.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR016159. Cullin_repeat-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
[Graphical view]
SMARTiSM00182. CULLIN. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF74788. SSF74788. 1 hit.
SSF75632. SSF75632. 1 hit.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60999-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMMTSTPTK RSVKLDDIWP ELEEGIYKII TDLNKGFPKQ KWIALYTHVY
60 70 80 90 100
DYCAASQSKS SAKVGMPKQQ ASGANYVGED LYNRLNLFLK KHMSQLLKLT
110 120 130 140 150
ETKMDEPLLN YYYTEWDRYT SAMKYINNIF QYMNRYWIKR EIDDGKKEVY
160 170 180 190 200
EIFILSLVIW RDCLFTPLKQ RLTNSLLDII ESERNGYQIN THLIKGVING
210 220 230 240 250
YVSLGLNREK PKETILQVYK SGFEELFLTA TENYYTNESA KFISENSVAD
260 270 280 290 300
YMKKVETRLN EEVKRVQQYL HQNTESELIA KCEKVLIEKH VEVIWNEFQT
310 320 330 340 350
LLEKDKIPDL TRMYSLLSRI PRGLEPLRTT LEKHVQNVGL QAVSSIATNG
360 370 380 390 400
VIEPKVYIET LLKVFKKYNE LVTGAFRSDT GFVASLDKAC RRFINENAVT
410 420 430 440 450
IAAKSSSKSP ELLARFTDFL LKKSPNNPEE SEMEQLLNDV MIVFKYIEDK
460 470 480 490 500
DVFQDFYSKM LAKRLIHGTS TSEDLEGTMI GKLKSTCGYE YTSKLQRMFT
510 520 530 540 550
DMSLSRELLD RFNNHIEQVE RSSLNIDFSV LVLATGSWPL QPPSTNFSIP
560 570 580 590 600
KELQACEQLF QKFYQNQHSG RKLNWLHHLS KGELKTKYLQ TSKSGYTLQC
610 620 630 640 650
STYQIGVLLQ FNQYETLTSE EIQESTQLID SVLKGTLTSL AKSKILLADP
660 670 680 690 700
PLDDEEIAKT TKFSLNKQFK NKKTKIFINV PVLTQVKEEI DSIHKTVEED
710 720 730 740 750
RKLQIQAAIV RIMKMRKQLA HSGLMTEVIS QLQTRFNPKV NIIKKCIDIL
760 770
IEKEYLMRVE GKKDHYSYVA
Length:770
Mass (Da):89,335
Last modified:August 1, 1998 - v1
Checksum:i4D14FFE07A0A08E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020287 mRNA. Translation: AAC15412.1.
AAFI02000187 Genomic DNA. Translation: EAL61342.1.
RefSeqiXP_629756.1. XM_629754.1.

Genome annotation databases

EnsemblProtistsiDDB0185191; DDB0185191; DDB_G0291972.
GeneIDi8628431.
KEGGiddi:DDB_G0291972.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020287 mRNA. Translation: AAC15412.1.
AAFI02000187 Genomic DNA. Translation: EAL61342.1.
RefSeqiXP_629756.1. XM_629754.1.

3D structure databases

ProteinModelPortaliO60999.
SMRiO60999. Positions 15-770.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0185191.

Proteomic databases

PaxDbiO60999.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0185191; DDB0185191; DDB_G0291972.
GeneIDi8628431.
KEGGiddi:DDB_G0291972.

Organism-specific databases

dictyBaseiDDB_G0291972. culA.

Phylogenomic databases

eggNOGiKOG2166. Eukaryota.
COG5647. LUCA.
InParanoidiO60999.
KOiK03347.
OMAiHIANQGL.
PhylomeDBiO60999.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-DDI-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DDI-69231. Cyclin D associated events in G1.
R-DDI-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiO60999.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR016159. Cullin_repeat-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
[Graphical view]
SMARTiSM00182. CULLIN. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF74788. SSF74788. 1 hit.
SSF75632. SSF75632. 1 hit.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Regulated protein degradation controls PKA function and cell-type differentiation in Dictyostelium."
    Mohanty S., Lee S., Yadava N., Dealy M.J., Johnson R.S., Firtel R.A.
    Genes Dev. 15:1435-1448(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, DISRUPTION PHENOTYPE.
    Strain: AX3.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiCUL1_DICDI
AccessioniPrimary (citable) accession number: O60999
Secondary accession number(s): Q54DW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: August 1, 1998
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.