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Protein

Cullin-1

Gene

culA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable core component of cullin-based SCF-like E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit (By similarity). Required at several stages during development. CulA and fbxA regulate multicellular development by targeting regA for degradation via a pathway that requires erkB function, leading to an increase in cAMP and PKA activity.By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: GO_Central
  • ubiquitin-protein transferase activity Source: dictyBase

GO - Biological processi

  • cell differentiation Source: dictyBase
  • chemotaxis Source: dictyBase
  • G1/S transition of mitotic cell cycle Source: dictyBase
  • sorocarp development Source: dictyBase
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-DDI-68949 Orc1 removal from chromatin
R-DDI-69231 Cyclin D associated events in G1
R-DDI-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-DDI-8951664 Neddylation
R-DDI-917937 Iron uptake and transport
R-DDI-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-1
Short name:
CUL-1
Alternative name(s):
Cullin-A
Gene namesi
Name:culA
Synonyms:cul1
ORF Names:DDB_G0291972
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291972 culA

Subcellular locationi

GO - Cellular componenti

  • SCF ubiquitin ligase complex Source: dictyBase

Pathology & Biotechi

Disruption phenotypei

Cells are defective in inducing cell-type-specific gene expression and exhibit defects during aggregation, including reduced chemotaxis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003450101 – 770Cullin-1Add BLAST770

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki714Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)By similarity

Post-translational modificationi

Neddylated; which enhances the ubiquitination activity of SCF.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO60999
PRIDEiO60999

Interactioni

Subunit structurei

Part of a complex that includes culA, fbxA and regA. Formation of this complex is dependent on the MAP kinase erkB.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi44689.DDB0185191

Structurei

3D structure databases

ProteinModelPortaliO60999
SMRiO60999
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2166 Eukaryota
COG5647 LUCA
InParanoidiO60999
KOiK03347
OMAiTTHKHIE
PhylomeDBiO60999

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR016157 Cullin_CS
IPR016158 Cullin_homology
IPR036317 Cullin_homology_sf
IPR001373 Cullin_N
IPR019559 Cullin_neddylation_domain
IPR016159 Cullin_repeat-like_dom_sf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00888 Cullin, 1 hit
PF10557 Cullin_Nedd8, 1 hit
SMARTiView protein in SMART
SM00182 CULLIN, 1 hit
SM00884 Cullin_Nedd8, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF74788 SSF74788, 1 hit
SSF75632 SSF75632, 1 hit
PROSITEiView protein in PROSITE
PS01256 CULLIN_1, 1 hit
PS50069 CULLIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

O60999-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMMTSTPTK RSVKLDDIWP ELEEGIYKII TDLNKGFPKQ KWIALYTHVY
60 70 80 90 100
DYCAASQSKS SAKVGMPKQQ ASGANYVGED LYNRLNLFLK KHMSQLLKLT
110 120 130 140 150
ETKMDEPLLN YYYTEWDRYT SAMKYINNIF QYMNRYWIKR EIDDGKKEVY
160 170 180 190 200
EIFILSLVIW RDCLFTPLKQ RLTNSLLDII ESERNGYQIN THLIKGVING
210 220 230 240 250
YVSLGLNREK PKETILQVYK SGFEELFLTA TENYYTNESA KFISENSVAD
260 270 280 290 300
YMKKVETRLN EEVKRVQQYL HQNTESELIA KCEKVLIEKH VEVIWNEFQT
310 320 330 340 350
LLEKDKIPDL TRMYSLLSRI PRGLEPLRTT LEKHVQNVGL QAVSSIATNG
360 370 380 390 400
VIEPKVYIET LLKVFKKYNE LVTGAFRSDT GFVASLDKAC RRFINENAVT
410 420 430 440 450
IAAKSSSKSP ELLARFTDFL LKKSPNNPEE SEMEQLLNDV MIVFKYIEDK
460 470 480 490 500
DVFQDFYSKM LAKRLIHGTS TSEDLEGTMI GKLKSTCGYE YTSKLQRMFT
510 520 530 540 550
DMSLSRELLD RFNNHIEQVE RSSLNIDFSV LVLATGSWPL QPPSTNFSIP
560 570 580 590 600
KELQACEQLF QKFYQNQHSG RKLNWLHHLS KGELKTKYLQ TSKSGYTLQC
610 620 630 640 650
STYQIGVLLQ FNQYETLTSE EIQESTQLID SVLKGTLTSL AKSKILLADP
660 670 680 690 700
PLDDEEIAKT TKFSLNKQFK NKKTKIFINV PVLTQVKEEI DSIHKTVEED
710 720 730 740 750
RKLQIQAAIV RIMKMRKQLA HSGLMTEVIS QLQTRFNPKV NIIKKCIDIL
760 770
IEKEYLMRVE GKKDHYSYVA
Length:770
Mass (Da):89,335
Last modified:August 1, 1998 - v1
Checksum:i4D14FFE07A0A08E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020287 mRNA Translation: AAC15412.1
AAFI02000187 Genomic DNA Translation: EAL61342.1
RefSeqiXP_629756.1, XM_629754.1

Genome annotation databases

EnsemblProtistsiEAL61342; EAL61342; DDB_G0291972
GeneIDi8628431
KEGGiddi:DDB_G0291972

Similar proteinsi

Entry informationi

Entry nameiCUL1_DICDI
AccessioniPrimary (citable) accession number: O60999
Secondary accession number(s): Q54DW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: August 1, 1998
Last modified: May 23, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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