O60993 (TRYS_CRIFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trypanothione synthetase
EC=6.3.1.9

Alternative name(s):
Cf-TS

Gene names

Name:

TRS

Organism

Crithidia fasciculata

Taxonomic identifier

5656 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Leishmaniinae › Crithidia

Protein attributes

Sequence length	652 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugates glutathione (gamma-Glu-Cys-Gly) and glutathionylspermidine to form trypanothione (N1,N(8)-bis(glutathionyl)spermidine), which is involved in maintaining intracellular thiol redox and in defense against oxidants.
Catalytic activity	Glutathione + glutathionylspermidine + ATP = N1,N(8)-bis-(glutathionyl)spermidine + ADP + phosphate.
Cofactor	Magnesium.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Contains 1 peptidase C51 domain.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW trypanothione synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 652	652	Trypanothione synthetase		PRO_0000070445
Regions
	Domain	34 – 174	141	Peptidase C51

Sequences

	Sequence		Length	Mass (Da)
	O60993 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 321BE90D39EEEA80	FASTA	652	74,517
	10 20 30 40 50 60 MASAERVPVS FNKPGRVPFG EVQGYAPGHI PAYSNKHDHF FSGERSIDDN VFCGFKYQCV 70 80 90 100 110 120 EFARRWLLER KGLVLPDVNW ACHIFKLKNV KDAATAEEVP VIAVRNGTEA KPEPDTLIIY 130 140 150 160 170 180 PSSDVNTVGH VGAITEVGDD YVCIADQNYR FHKWEASYSY KLKLQHKDGV WTIIDDIDPN 190 200 210 220 230 240 DVEIPLGWVT FPGYENRPEG AAPPALHPSL HFQPPEEPYL VRKTYEPTET KANWLDLNDP 250 260 270 280 290 300 AEKLFVEEFG MDVSRSRLEE TTVNYYECDH EFHLRCIAYG TQLHDYFMEA TAQVINDDER 310 320 330 340 350 360 LRIFKIPEEL WPRMRHSWKY QQTYISGRFD FAYNNETHQM KCFEYNADSA STLLECGRIQ 370 380 390 400 410 420 QKWAESVGLD KEGTRGSGWA VERNLRTAWA TCGATGRVHF LVDDEKEEQY TALYCLQAAE 430 440 450 460 470 480 AVGLEGKLCV MYDEFRFNEE GYVVDSDGVR VRNIWKTWMW ESAISDYFAA QAERGADWKA 490 500 510 520 530 540 TPADKVRLCD LMLGKDWDIL YFEPMWKLIP SNKAILPIIY HNHPDHPAIL RAEYELTDEL 550 560 570 580 590 600 RAVGYARKPI VGRVGRNVTI TDGTGEVHAE SGGNFGERDM IYQELFSLTK QDGYYAIIGG 610 620 630 640 650 WMLGDAFSGT GIREDKSIIT GLDSPFAAIR IKINAIPRPL THKDLDKLAE DE

References

[1]	"Cloning and characterization of the two enzymes responsible for trypanothione biosynthesis in Crithidia fasciculata." Tetaud E., Manai F., Barrett M.P., Nadeau K., Walsh C.T., Fairlamb A.H. J. Biol. Chem. 273:19383-19390(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 168-191; 232-255; 437-450; 457-474; 592-610 AND 617-630. Strain: HS6.
[2]	"Purification of glutathionylspermidine and trypanothione synthetases from Crithidia fasciculata." Smith K., Nadeau K., Bradley M., Walsh C., Fairlamb A.H. Protein Sci. 1:874-883(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 168-191; 232-255; 437-450; 457-474; 592-610 AND 617-630, CHARACTERIZATION.
[3]	"Convenient isolation and kinetic mechanism of glutathionylspermidine synthetase from Crithidia fasciculata." Koenig K., Menge U., Kiess M., Wray V., Flohe L. J. Biol. Chem. 272:11908-11915(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 17-36; 224-229; 235-242; 515-533; 550-561 AND 617-629, CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF006615 Genomic DNA. Translation: AAC39132.1.
3D structure databases
ProteinModelPortal	O60993.
SMR	O60993. Positions 1-632.
ModBase	Search...
Protein family/group databases
MEROPS	C51.A01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
SABIO-RK	O60993.
Family and domain databases
InterPro	IPR007921. CHAP. IPR005494. GSPS_pre-ATP-grasp-like_dom. IPR016185. PreATP-grasp_dom. [Graphical view]
Pfam	PF05257. CHAP. 1 hit. PF03738. GSP_synth. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
PROSITE	PS50911. CHAP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYS_CRIFA

Accession

Primary (citable) accession number: O60993

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	August 1, 1998
Last modified:	April 3, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)