O60936 (NOL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleolar protein 3

Alternative name(s):
Apoptosis repressor with CARD
Muscle-enriched cytoplasmic protein
Short name=Myp
Nucleolar protein of 30 kDa
Short name=Nop30

Gene names

Name:	NOL3
Synonyms:	ARC, NOP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Isoform 1 may be involved in RNA splicing. Isoform 2 may inhibit apoptosis.
Subunit structure	Interacts with TFPT By similarity. Isoform 1 oligomerizes and binds to SFRS9/SRp30C and also interacts with NPM1. Isoform 2 binds CASP2/caspase-2 and CASP8/caspase-8 and inhibits caspase-8 activity. Ref.1
Subcellular location	Isoform 1: Nucleus › nucleolus. Isoform 2: Cytoplasm.
Tissue specificity	Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.
Sequence similarities	Contains 1 CARD domain.

Ontologies

Keywords
Biological process	Apoptosis mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA splicing Traceable author statement. Source: ProtInc anti-apoptosis Traceable author statement. Source: ProtInc apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Traceable author statement. Source: ProtInc nucleolus Traceable author statement. Source: ProtInc
Molecular function	RNA binding Traceable author statement. Source: ProtInc identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-740992,EBI-740992
CASP8	Q14790	3	EBI-740992,EBI-78060

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O60936-1)

Also known as: Nop30;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O60936-2)

Also known as: Myp;

The sequence of this isoform differs from the canonical sequence as follows:
     96-219: ATGTAAMTLH...SDRRCPAHAG → VGPGYRDRSY...FEERDESEDS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Nucleolar protein 3		PRO_0000144099
Regions
	Domain	4 – 95	92	CARD
Natural variations
	Alternative sequence	96 – 219	124	ATGTA…PAHAG → VGPGYRDRSYDPPCPGHWTP EAPGSGTTCPGLPRASDPDE AGGPEGSEAVQSGTPEEPEP ELEAEASKEAEPEPEPEPEL EPEAEAEPEPELEPEPDPEP EPDFEERDESEDS in isoform 2.		VSP_000789

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Nop30) [UniParc]. Last modified August 1, 1998. Version 1. Checksum: B1CCCB199D4FEE09	FASTA	219	24,327
	10 20 30 40 50 60 MGNAQERPSE TIDRERKRLV ETLQADSGLL LDALLARGVL TGPEYEALDA LPDAERRVRR 70 80 90 100 110 120 LLLLVQGKGE AACQELLRCA QRTAGAPDPA WDWQHATGTA AMTLHAQATG RRRHPARGPH 130 140 150 160 170 180 APGCPELQTL TRPGALRAPR RCNPGPRRSQ SQSWKLRPLK RLNRSRSQSQ SWNPRLKQNQ 190 200 210 SRNWSQNRTQ SPSPTSRKGT SPKIPEGQSS DRRCPAHAG
	Isoform 2 (Myp) [UniParc]. Checksum: 239EF1A143EF6168	FASTA	208	22,629
	10 20 30 40 50 60 MGNAQERPSE TIDRERKRLV ETLQADSGLL LDALLARGVL TGPEYEALDA LPDAERRVRR 70 80 90 100 110 120 LLLLVQGKGE AACQELLRCA QRTAGAPDPA WDWQHVGPGY RDRSYDPPCP GHWTPEAPGS 130 140 150 160 170 180 GTTCPGLPRA SDPDEAGGPE GSEAVQSGTP EEPEPELEAE ASKEAEPEPE PEPELEPEAE 190 200 AEPEPELEPE PDPEPEPDFE ERDESEDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases." Koseki T., Inohara N., Chen S., Nunez G. Proc. Natl. Acad. Sci. U.S.A. 95:5156-5160(1998) [PubMed: 9560245] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH CASP2; CASP8; NPM1 AND SFRS9.
[2]	"Alternative splicing determines the intracellular localization of the novel nuclear protein Nop30 and its interaction with the splicing factor SRp30c." Stoss O., Schwaiger F.-W., Cooper T.A., Stamm S. J. Biol. Chem. 274:10951-10962(1999) [PubMed: 10196175] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2). Tissue: Cervix carcinoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF043244 mRNA. Translation: AAC34993.1. AF064598 Genomic DNA. Translation: AAC18590.1. AF064598 Genomic DNA. Translation: AAC18591.1. AF064599 mRNA. Translation: AAC18593.1. AF064600 mRNA. Translation: AAC18594.1. BC012798 mRNA. Translation: AAH12798.1.
IPI	IPI00105916. IPI00216346.
RefSeq	NP_001171986.1. NM_001185057.1. NP_001171987.1. NM_001185058.1. NP_003937.1. NM_003946.4.
UniGene	Hs.513667.
3D structure databases
ProteinModelPortal	O60936.
SMR	O60936. Positions 5-84.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29940N.
IntAct	O60936. 7 interactions.
MINT	MINT-1462750.
STRING	O60936.
Proteomic databases
PeptideAtlas	O60936.
PRIDE	O60936.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000445652; ENSP00000403112; ENSG00000140939.
GeneID	8996.
KEGG	hsa:8996.
UCSC	uc002erq.1. human.
Organism-specific databases
CTD	8996.
GeneCards	GC16P067207.
H-InvDB	HIX0202303.
HGNC	HGNC:7869. NOL3.
HPA	CAB009208.
MIM	605235. gene.
neXtProt	NX_O60936.
PharmGKB	PA31673.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG14858.
GeneTree	ENSGT00510000049353.
Gene expression databases
ArrayExpress	O60936.
CleanEx	HS_ARC. HS_NOL3.
Genevestigator	O60936.
GermOnline	ENSG00000140939. Homo sapiens.
Family and domain databases
InterPro	IPR001315. CARD. IPR011029. DEATH-like. [Graphical view]
Gene3D	G3DSA:1.10.533.10. DEATH_like. 1 hit.
Pfam	PF00619. CARD. 1 hit. [Graphical view]
SMART	SM00114. CARD. 1 hit. [Graphical view]
SUPFAM	SSF47986. DEATH_like. 1 hit.
PROSITE	PS50209. CARD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	33735.
SOURCE	Search...

Entry information

Entry name

NOL3_HUMAN

Accession

Primary (citable) accession number: O60936
Secondary accession number(s): O60937

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.