Reviewed, UniProtKB/Swiss-Prot O60936 (NOL3_HUMAN)

Names and origin

Protein names

Recommended name:
    Nucleolar protein 3
Alternative name(s):
    Apoptosis repressor with CARD
    Muscle-enriched cytoplasmic protein
      Short name=Myp
    Nucleolar protein of 30 kDa
      Short name=Nop30

Gene names

Name:	NOL3
Synonyms:	ARC, NOP

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The nuclear 1/Nop30) may be involved in RNA splicing and the cytoplasmic 2/Myp) may inhibit apoptosis.
Subunit structure	Interacts with TFPT By similarity. Isoform 1 oligomerizes and binds to SFRS9/SRp30C and also interacts with NPM1. Isoform 2 binds CASP2/caspase-2 and CASP8/caspase-8 and inhibits caspase-8 activity.
Subcellular location	Isoform 1: Nucleus › nucleolus. Isoform 2: Cytoplasm.
Tissue specificity	Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.
Sequence similarities	Contains 1 CARD domain.

Ontologies

Keywords
Biological process	Apoptosis mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA splicing Ref.2 Traceable author statement. Source: ProtInc anti-apoptosis Ref.1 Traceable author statement. Source: ProtInc apoptosis Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Ref.2 Traceable author statement. Source: ProtInc nucleolus Ref.2 Traceable author statement. Source: ProtInc
Molecular function	RNA binding Ref.2 Traceable author statement. Source: ProtInc identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-740992,EBI-740992
CASP8	Q14790	3	EBI-740992,EBI-78060
PACSIN3	Q9UKS6	1	EBI-740992,EBI-77926
PNKP	Q96T60	1	EBI-740992,EBI-1045072

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O60936-1)

Also known as: Nop30;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O60936-2)

Also known as: Myp;

The sequence of this isoform differs from the canonical sequence as follows:
     96-219: ATGTAAMTLH...SDRRCPAHAG → VGPGYRDRSY...FEERDESEDS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Nucleolar protein 3		PRO_0000144099
Regions
	Domain	4 – 95	92	CARD
Natural variations
	Alternative sequence	96 – 219	124	ATGTA…PAHAG → VGPGYRDRSYDPPCPGHWTP EAPGSGTTCPGLPRASDPDE AGGPEGSEAVQSGTPEEPEP ELEAEASKEAEPEPEPEPEL EPEAEAEPEPELEPEPDPEP EPDFEERDESEDS in isoform 2.		VSP_000789

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Nop30) [UniParc]. Last modified August 1, 1998. Version 1. Checksum: B1CCCB199D4FEE09	FASTA	219	24,327
	10 20 30 40 50 60 MGNAQERPSE TIDRERKRLV ETLQADSGLL LDALLARGVL TGPEYEALDA LPDAERRVRR 70 80 90 100 110 120 LLLLVQGKGE AACQELLRCA QRTAGAPDPA WDWQHATGTA AMTLHAQATG RRRHPARGPH 130 140 150 160 170 180 APGCPELQTL TRPGALRAPR RCNPGPRRSQ SQSWKLRPLK RLNRSRSQSQ SWNPRLKQNQ 190 200 210 SRNWSQNRTQ SPSPTSRKGT SPKIPEGQSS DRRCPAHAG
	Isoform 2 (Myp). Checksum: 239EF1A143EF6168	FASTA	208	22,629
	10 20 30 40 50 60 MGNAQERPSE TIDRERKRLV ETLQADSGLL LDALLARGVL TGPEYEALDA LPDAERRVRR 70 80 90 100 110 120 LLLLVQGKGE AACQELLRCA QRTAGAPDPA WDWQHVGPGY RDRSYDPPCP GHWTPEAPGS 130 140 150 160 170 180 GTTCPGLPRA SDPDEAGGPE GSEAVQSGTP EEPEPELEAE ASKEAEPEPE PEPELEPEAE 190 200 AEPEPELEPE PDPEPEPDFE ERDESEDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases." Koseki T., Inohara N., Chen S., Nunez G. Proc. Natl. Acad. Sci. U.S.A. 95:5156-5160(1998) [PubMed: 9560245] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH CASP2; CASP8; NPM1 AND SFRS9.
[2]	"Alternative splicing determines the intracellular localization of the novel nuclear protein Nop30 and its interaction with the splicing factor SRp30c." Stoss O., Schwaiger F.-W., Cooper T.A., Stamm S. J. Biol. Chem. 274:10951-10962(1999) [PubMed: 10196175] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2). Tissue: Cervix carcinoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF043244 mRNA. Translation: AAC34993.1. AF064598 Genomic DNA. Translation: AAC18590.1. AF064598 Genomic DNA. Translation: AAC18591.1. AF064599 mRNA. Translation: AAC18593.1. AF064600 mRNA. Translation: AAC18594.1. BC012798 mRNA. Translation: AAH12798.1.
IPI	IPI00105916. IPI00216346.
RefSeq	NP_003937.1.
UniGene	Hs.513667
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O60936. 8 interactions.
Proteomic databases
PeptideAtlas	O60936.
Genome annotation databases
GeneID	8996.
KEGG	hsa:8996.
UCSC	uc002erq.1. human.
Organism-specific databases
GeneCards	GC16P065765.
H-InvDB	HIX0013136.
HGNC	HGNC:7869. NOL3.
MIM	605235. gene.
PharmGKB	PA31673.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O60936.
HOVERGEN	O60936.
OMA	O60936. RERSYDP.
Gene expression databases
CleanEx	HS_ARC. HS_NOL3.
GermOnline	ENSG00000140939. Homo sapiens.
Family and domain databases
InterPro	IPR001315. CARD. [Graphical view]
Pfam	PF00619. CARD. 1 hit. [Graphical view]
SMART	SM00114. CARD. 1 hit. [Graphical view]
PROSITE	PS50209. CARD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	33735.
SOURCE	Search...

Entry information

Entry name

NOL3_HUMAN

Accession

Primary (citable) accession number: O60936
Secondary accession number(s): O60937

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	August 1, 1998
Last modified:	July 7, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)