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Reviewed, UniProtKB/Swiss-Prot O60909 (B4GT2_HUMAN)

Last modified July 7, 2009. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Beta-1,4-galactosyltransferase 2
      Short name=Beta-1,4-GalTase 2
      Short name=Beta4Gal-T2
      Short name=b4Gal-T2
    EC=2.4.1.-
Alternative name(s):
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2
Including the following 4 domains:
    1- Recommended name:
            Lactose synthase A protein
              EC=2.4.1.22
    2- Recommended name:
            N-acetyllactosamine synthase
              EC=2.4.1.90
        Alternative name(s):
            Nal synthetase
    3- Recommended name:
            Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase
              EC=2.4.1.38
    4- Recommended name:
            Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
              EC=2.4.1.-
Gene names
Name: B4GALT2
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose.

Catalytic activity

UDP-galactose + D-glucose = UDP + lactose.

UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.

UDP-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactor

Manganese By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein. Note: Trans cisternae of Golgi stack.

Tissue specificity

Weakly expressed in various tissues. Highest expression in prostate, testis, ovary, intestine, muscle, and in fetal brain.

Sequence similarities

Belongs to the glycosyltransferase 7 family.

Biophysicochemical properties

Kinetic parameters:

KM=71 µM for GlcNAc-B-S-pNP

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O60909-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O60909-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-118: Missing.
     119-119: E → MPSTQLLAAAAAAATAPGPTPPPLAPGSLRSPVPCPVPRLPRCHPVLTRHLVL
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Beta-1,4-galactosyltransferase 2
PRO_0000080533

Regions

Topological domain1 – 1515Cytoplasmic Potential
Transmembrane16 – 3621Signal-anchor for type II membrane protein Potential
Topological domain37 – 372336Lumenal Potential

Sites

Metal binding2181Manganese By similarity
Metal binding3111Manganese By similarity

Amino acid modifications

Glycosylation661N-linked (GlcNAc...) Potential
Glycosylation711N-linked (GlcNAc...) Potential
Glycosylation3571N-linked (GlcNAc...) Potential
Disulfide bond97 ↔ 139 By similarity
Disulfide bond211 ↔ 230 By similarity

Natural variations

Alternative sequence1 – 118118Missing in isoform 2.
VSP_014103
Alternative sequence1191E → MPSTQLLAAAAAAATAPGPT PPPLAPGSLRSPVPCPVPRL PRCHPVLTRHLVL in isoform 2.
VSP_014104
Natural variant1221Q → H: dbSNP rs1859728. Ref.5
VAR_020487
Natural variant3381G → R: dbSNP rs35904809.
VAR_054021

Experimental info

Sequence conflict1001S → TS Ref.2
Sequence conflict1361P → S in AAC39733. Ref.2
Sequence conflict1771G → C in AAC39733. Ref.2
Sequence conflict315 – 3162KH → ND in AAC39733. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: A6F7800251AA1E4F

FASTA37241,972
        10         20         30         40         50         60 
MSRLLGGTLE RVCKAVLLLC LLHFLVAVIL YFDVYAQHLA FFSRFSARGP AHALHPAASS 

        70         80         90        100        110        120 
SSSSSNCSRP NATASSSGLP EVPSALPGPT APTLPPCPDS PPGLVGRLLI EFTSPMPLER 

       130        140        150        160        170        180 
VQRENPGVLM GGRYTPPDCT PAQTVAVIIP FRHREHHLRY WLHYLHPILR RQRLRYGVYV 

       190        200        210        220        230        240 
INQHGEDTFN RAKLLNVGFL EALKEDAAYD CFIFSDVDLV PMDDRNLYRC GDQPRHFAIA 

       250        260        270        280        290        300 
MDKFGFRLPY AGYFGGVSGL SKAQFLRING FPNEYWGWGG EDDDIFNRIS LTGMKISRPD 

       310        320        330        340        350        360 
IRIGRYRMIK HDRDKHNEPN PQRFTKIQNT KLTMKRDGIG SVRYQVLEVS RQPLFTNITV 

       370 
DIGRPPSWPP RG 

« Hide

Isoform 2.

Checksum: A483868104528A2B
Show »

FASTA30634,883

References

« Hide 'large scale' references
[1]"A family of human beta4-galactosyltransferases. Cloning and expression of two novel UDP-galactose:beta-n-acetylglucosamine beta1, 4-galactosyltransferases, beta4Gal-T2 and beta4Gal-T3."
Almeida R., Amado M., David L., Levery S.B., Holmes E.H., Merkx G., van Kessel A.G., Rygaard E., Hassan H., Bennett E., Clausen H.
J. Biol. Chem. 272:31979-31991(1997) [PubMed: 9405390] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"The expanding beta 4-galactosyltransferase gene family: messages from the databanks."
Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L.
Glycobiology 8:517-526(1998) [PubMed: 9597550] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells."
Guo S., Sato T., Shirane K., Furukawa K.
Glycobiology 11:813-820(2001) [PubMed: 11588157] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), BIOPHYSICOCHEMICAL PROPERTIES.
Tissue: Erythroleukemia.
[4]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed: 16710414] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT HIS-122.
Tissue: Chondrosarcoma.
[6]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 149-372 (ISOFORMS 1/2).
Tissue: Uterus.
[7]"Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions."
Amado M., Almeida R., Schwientek T., Clausen H.
Biochim. Biophys. Acta 1473:35-53(1999) [PubMed: 10580128] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Beta-1,4-galactosyltransferase 2

Cross-references

Sequence databases

Y12510 mRNA. Translation: CAA73112.1.
AF038660 mRNA. Translation: AAC39733.1.
AB024434 mRNA. Translation: BAA75819.1.
AL139220, AL357079 Genomic DNA. Translation: CAI19431.1.
AL139220, AL357079 Genomic DNA. Translation: CAI19432.1.
AL357079, AL139220 Genomic DNA. Translation: CAI16802.1.
AL357079, AL139220 Genomic DNA. Translation: CAI16803.1.
BC096821 mRNA. Translation: AAH96821.1.
AL137647 mRNA. Translation: CAB70857.1.
IPIIPI00033031.
IPI00386793.
PIRT46511.
RefSeqNP_001005417.1.
NP_003771.1.
UniGeneHs.632403

3D structure databases

HSSPHSSP built from PDB template 1NMM based on UniProtKB P08037.
ModBaseSearch...

Protein family/group databases

CAZyGT7. Glycosyltransferase Family 7.

Proteomic databases

PRIDEO60909.

Genome annotation databases

EnsemblENSG00000117411. Homo sapiens. [Contig view]
GeneID8704.
KEGGhsa:8704.
UCSCuc001clg.1. human.
uc001clh.1. human.

Organism-specific databases

GeneCardsGC01P044217.
H-InvDBHIX0000514.
HGNCHGNC:925. B4GALT2.
MIM604013. gene.
PharmGKBPA25224.
GenAtlasSearch...

Phylogenomic databases

HOVERGENO60909.
OMAO60909. YVINQHG.

Enzyme and pathway databases

BRENDA2.4.1.22. 247.
2.4.1.38. 247.
2.4.1.90. 247.

Gene expression databases

ArrayExpressO60909.
BgeeO60909.
CleanExHS_B4GALT2.
GermOnlineENSG00000117411. Homo sapiens.

Family and domain databases

InterProIPR003859. Galactosyl_T_2.
[Graphical view]
PANTHERPTHR19300. Galactosyl_T_2. 1 hit.
PfamPF02709. Galactosyl_T_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

DrugBankDB00141. N-Acetyl-D-glucosamine.
NextBio32635.
SOURCESearch...

Entry information

Entry nameB4GT2_HUMAN
AccessionPrimary (citable) accession number: O60909
Secondary accession number(s): O60511 expand/collapse secondary AC list , Q4V9L9, Q5T4X5, Q5T4Y5, Q9BUP6, Q9NSY7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 1, 1998
Last modified: July 7, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents