O60907 (TBL1X_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-box-like/WD repeat-containing protein TBL1X

Alternative name(s):
SMAP55
Transducin beta-like protein 1X
Transducin-beta-like protein 1, X-linked

Gene names

Name:	TBL1X
Synonyms:	TBL1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of corepressor complexes that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of transcription repressor complexes, thereby allowing cofactor exchange. Ref.10
Subunit structure	Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Component of a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Probably part of other corepressor complexes, that do not contain NCOR1 and NCOR2. Interacts with histones H2B, H3a and H4. Ref.7
Subcellular location	Nucleus Probable.
Tissue specificity	Ubiquitous. Ref.1
Domain	The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes By similarity.
Sequence similarities	Belongs to the WD repeat EBI family. Contains 1 F-box-like domain. Contains 1 LisH domain. Contains 8 WD repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Disease	Deafness
Domain	Repeat WD repeat
Molecular function	Activator
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Notch signaling pathway Traceable author statement. Source: Reactome canonical Wnt receptor signaling pathway Inferred from mutant phenotype PubMed 18193033. Source: UniProtKB cellular lipid metabolic process Traceable author statement. Source: Reactome negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.9. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 18193033. Source: UniProtKB proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB sensory perception of sound Inferred from mutant phenotype Ref.1. Source: UniProtKB small molecule metabolic process Traceable author statement. Source: Reactome transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	spindle microtubule Inferred from direct assay PubMed 18326024. Source: UniProtKB transcriptional repressor complex Inferred from direct assay Ref.9PubMed 15601853. Source: UniProtKB
Molecular_function	histone binding Inferred from direct assay PubMed 15601853. Source: UniProtKB transcription corepressor activity Inferred from direct assay Ref.9. Source: UniProtKB transcription regulatory region DNA binding Inferred from direct assay PubMed 18193033. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O60907-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O60907-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 577

577

F-box-like/WD repeat-containing protein TBL1X

PRO_0000051263

Regions

Domain

55 – 87

33

LisH

Domain

92 – 137

46

F-box-like

Repeat

230 – 269

40

WD 1

Repeat

286 – 325

40

WD 2

Repeat

327 – 366

40

WD 3

Repeat

369 – 409

41

WD 4

Repeat

410 – 449

40

WD 5

Repeat

452 – 500

49

WD 6

Repeat

503 – 542

40

WD 7

Repeat

544 – 576

33

WD 8

Compositional bias

159 – 175

17

Poly-Ala

Natural variations

Alternative sequence

1 – 51

51

Missing in isoform 2.

VSP_036905

Experimental info

Sequence conflict

316

1

T → A in BAF82098. Ref.2

Sequence conflict

390

1

T → A in BAF83651. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

577

Helix Strand Turn

Details...

Helix

56 – 69

14

Helix

73 – 82

10

Helix

85 – 87

3

Helix

92 – 94

3

Helix

99 – 116

18

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 14, 2009. Version 3. Checksum: D830A37781E2A15C	FASTA	577	62,496
	10 20 30 40 50 60 MTELAGASSS CCHRPAGRGA MQSVLHHFQR LRGREGGSHF INTSSPRGEA KMSITSDEVN 70 80 90 100 110 120 FLVYRYLQES GFSHSAFTFG IESHISQSNI NGTLVPPAAL ISILQKGLQY VEAEISINED 130 140 150 160 170 180 GTVFDGRPIE SLSLIDAVMP DVVQTRQQAF REKLAQQQAS AAAAAAAATA AATAATTTSA 190 200 210 220 230 240 GVSHQNPSKN REATVNGEEN RAHSVNNHAK PMEIDGEVEI PSSKATVLRG HESEVFICAW 250 260 270 280 290 300 NPVSDLLASG SGDSTARIWN LNENSNGGST QLVLRHCIRE GGHDVPSNKD VTSLDWNTNG 310 320 330 340 350 360 TLLATGSYDG FARIWTEDGN LASTLGQHKG PIFALKWNRK GNYILSAGVD KTTIIWDAHT 370 380 390 400 410 420 GEAKQQFPFH SAPALDVDWQ NNTTFASCST DMCIHVCRLG CDRPVKTFQG HTNEVNAIKW 430 440 450 460 470 480 DPSGMLLASC SDDMTLKIWS MKQEVCIHDL QAHNKEIYTI KWSPTGPATS NPNSNIMLAS 490 500 510 520 530 540 ASFDSTVRLW DIERGVCTHT LTKHQEPVYS VAFSPDGKYL ASGSFDKCVH IWNTQSGNLV 550 560 570 HSYRGTGGIF EVCWNARGDK VGASASDGSV CVLDLRK
	Isoform 2 [UniParc]. Checksum: 98922F88EC42F6E9	FASTA	526	57,049
	10 20 30 40 50 60 MSITSDEVNF LVYRYLQESG FSHSAFTFGI ESHISQSNIN GTLVPPAALI SILQKGLQYV 70 80 90 100 110 120 EAEISINEDG TVFDGRPIES LSLIDAVMPD VVQTRQQAFR EKLAQQQASA AAAAAAATAA 130 140 150 160 170 180 ATAATTTSAG VSHQNPSKNR EATVNGEENR AHSVNNHAKP MEIDGEVEIP SSKATVLRGH 190 200 210 220 230 240 ESEVFICAWN PVSDLLASGS GDSTARIWNL NENSNGGSTQ LVLRHCIREG GHDVPSNKDV 250 260 270 280 290 300 TSLDWNTNGT LLATGSYDGF ARIWTEDGNL ASTLGQHKGP IFALKWNRKG NYILSAGVDK 310 320 330 340 350 360 TTIIWDAHTG EAKQQFPFHS APALDVDWQN NTTFASCSTD MCIHVCRLGC DRPVKTFQGH 370 380 390 400 410 420 TNEVNAIKWD PSGMLLASCS DDMTLKIWSM KQEVCIHDLQ AHNKEIYTIK WSPTGPATSN 430 440 450 460 470 480 PNSNIMLASA SFDSTVRLWD IERGVCTHTL TKHQEPVYSV AFSPDGKYLA SGSFDKCVHI 490 500 510 520 WNTQSGNLVH SYRGTGGIFE VCWNARGDKV GASASDGSVC VLDLRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"X-linked late-onset sensorineural deafness caused by a deletion involving OA1 and a novel gene containing WD-40 repeats." Bassi M.T., Ramesar R.S., Caciotti B., Winship I.M., De Grandi A., Riboni M., Townes P.L., Beighton P., Ballabio A., Borsani G. Am. J. Hum. Genet. 64:1604-1616(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[3]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Lymph and Pancreas.
[5]	"A core SMRT corepressor complex containing HDAC3 and TBL1, a WD40-repeat protein linked to deafness." Guenther M.G., Lane W.S., Fischle W., Verdin E., Lazar M.A., Shiekhattar R. Genes Dev. 14:1048-1057(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF THE N-COR COMPLEX WITH NCOR2 AND HDAC3.
[6]	"Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3." Li J., Wang J., Wang J., Nawaz Z., Liu J.M., Qin J., Wong J. EMBO J. 19:4342-4350(2000) [PubMed] [Europe PMC] [Abstract] Cited for: COMPONENT OF THE N-COR COMPLEX WITH NCOR2 AND HDAC3.
[7]	"Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses." Matsuzawa S., Reed J.C. Mol. Cell 7:915-926(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT OF A COMPLEX WITH UBE2D1; CACYBP; SIAH1 AND APC.
[8]	"The N-CoR-HDAC3 nuclear receptor corepressor complex inhibits the JNK pathway through the integral subunit GPS2." Zhang J., Kalkum M., Chait B.T., Roeder R.G. Mol. Cell 9:611-623(2002) [PubMed] [Europe PMC] [Abstract] Cited for: COMPONENT OF THE N-COR COMPLEX WITH NCOR1; NCOR2; GPS2; TBL1R AND HDAC3.
[9]	"Purification and functional characterization of the human N-CoR complex: the roles of HDAC3, TBL1 and TBLR1." Yoon H.-G., Chan D.W., Huang Z.-Q., Li J., Fondell J.D., Qin J., Wong J. EMBO J. 22:1336-1346(2003) [PubMed] [Europe PMC] [Abstract] Cited for: COMPONENT OF THE N-COR COMPLEX WITH TBL1R; CORO2A AND HDAC3, HISTONE-BINDING.
[10]	"A corepressor/coactivator exchange complex required for transcriptional activation by nuclear receptors and other regulated transcription factors." Perissi V., Aggarwal A., Glass C.K., Rose D.W., Rosenfeld M.G. Cell 116:511-526(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, RECRUITMENT OF 19S PROTEASOME COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y12781 mRNA. Translation: CAA73319.1.
AK289409 mRNA. Translation: BAF82098.1.
AK290962 mRNA. Translation: BAF83651.1.
AC003036 Genomic DNA. No translation available.
BC032708 mRNA. Translation: AAH32708.1.
BC052304 mRNA. Translation: AAH52304.1.

IPI

IPI00218132.
IPI00640917.

RefSeq

NP_001132938.1. NM_001139466.1.
NP_001132939.1. NM_001139467.1.
NP_001132940.1. NM_001139468.1.
NP_005638.1. NM_005647.3.

UniGene

Hs.495656.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2XTC	X-ray	2.22	A/B	52-141	[»]
2XTD	X-ray	3.20	A/B	52-122	[»]
2XTE	X-ray	3.90	A/B/C/D/E/F/G/H/I/J/K/L	52-141	[»]

ProteinModelPortal

O60907.

ModBase

Search...

Protein-protein interaction databases

IntAct

O60907. 6 interactions.

STRING

9606.ENSP00000217964.

PTM databases

PhosphoSite

O60907.

Proteomic databases

PaxDb

O60907.

PRIDE

O60907.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000217964; ENSP00000217964; ENSG00000101849.
ENST00000380961; ENSP00000370348; ENSG00000101849.
ENST00000407597; ENSP00000385988; ENSG00000101849.
ENST00000424279; ENSP00000394097; ENSG00000101849.
ENST00000536365; ENSP00000445317; ENSG00000101849.

GeneID

6907.

KEGG

hsa:6907.

UCSC

uc004csq.4. human.

Organism-specific databases

CTD

6907.

GeneCards

GC0XP009431.

HGNC

HGNC:11585. TBL1X.

HPA

CAB005363.

MIM

300196. gene.

neXtProt

NX_O60907.

PharmGKB

PA36349.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG2319.

HOGENOM

HOG000220902.

HOVERGEN

HBG050240.

InParanoid

O60907.

KO

K04508.

OMA

RAHSVNN.

OrthoDB

EOG412M5M.

PhylomeDB

O60907.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.
REACT_111217. Metabolism.

Gene expression databases

ArrayExpress

O60907.

Bgee

O60907.

CleanEx

HS_TBL1X.

Genevestigator

O60907.

GermOnline

ENSG00000101849. Homo sapiens.

Family and domain databases

Gene3D

2.130.10.10. 2 hits.

InterPro

IPR020472. G-protein_beta_WD-40_rep.
IPR006594. LisH_dimerisation.
IPR013720. LisH_dimerisation_subgr.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]

Pfam

PF08513. LisH. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]

PRINTS

PR00320. GPROTEINBRPT.

SMART

SM00667. LisH. 1 hit.
SM00320. WD40. 8 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS50896. LISH. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

TBL1X. human.

EvolutionaryTrace

O60907.

GenomeRNAi

6907.

NextBio

27011.

SOURCE

Search...

Entry information

Entry name

TBL1X_HUMAN

Accession

Primary (citable) accession number: O60907
Secondary accession number(s): A8K044, A8K4J7, Q86UY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	April 14, 2009
Last modified:	May 1, 2013
This is version 125 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.