O60896 (RAMP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Receptor activity-modifying protein 3

Alternative name(s):
Calcitonin-receptor-like receptor activity-modifying protein 3
Short name=CRLR activity-modifying protein 3

Gene names

Name:

RAMP3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for adrenomedullin (AM) together with CALCRL. Ref.1
Subunit structure	Heterodimer of CALCRL and RAMP3 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Strongly expressed in lung, breast, immune system and fetal tissues. Ref.1
Sequence similarities	Belongs to the RAMP family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara calcium ion transport Inferred from direct assay PubMed 10882736. Source: UniProtKB intracellular protein transport Inferred from electronic annotation. Source: InterPro negative regulation of transcription, DNA-dependent Inferred from mutant phenotype PubMed 14722252. Source: UniProtKB positive regulation of receptor recycling Inferred from direct assay PubMed 15613468. Source: UniProtKB protein localization to plasma membrane Inferred from direct assay PubMed 10882736. Source: UniProtKB receptor internalization Inferred from direct assay PubMed 10882736PubMed 15613468. Source: UniProtKB regulation of G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	cell surface Inferred from direct assay PubMed 10882736PubMed 14722252. Source: UniProtKB integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc lysosome Traceable author statement PubMed 10882736. Source: ProtInc receptor complex Inferred from direct assay PubMed 15613468. Source: UniProtKB
Molecular_function	coreceptor activity Inferred from electronic annotation. Source: Compara protein transporter activity Inferred from direct assay PubMed 10882736Ref.1. Source: UniProtKB receptor activity Inferred from direct assay PubMed 10882736. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 148	125	Receptor activity-modifying protein 3		PRO_0000030176
Regions
	Topological domain	24 – 118	95	Extracellular Potential
	Transmembrane	119 – 138	20	Helical; Potential
	Topological domain	139 – 148	10	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	58	1	N-linked (GlcNAc...) Potential
	Glycosylation	71	1	N-linked (GlcNAc...) Potential
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Disulfide bond	40 ↔ 72		By similarity
	Disulfide bond	57 ↔ 104		By similarity
Natural variations
	Natural variant	26	1	G → D. Corresponds to variant rs10272187 [ dbSNP | Ensembl ].		VAR_034437
	Natural variant	33	1	M → L. Ref.4 Corresponds to variant rs11550711 [ dbSNP | Ensembl ].		VAR_053628
	Natural variant	56	1	W → R. Corresponds to variant rs2074654 [ dbSNP | Ensembl ].		VAR_024602

Sequences

	Sequence		Length	Mass (Da)
	O60896 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: EEEE312496EF513C	FASTA	148	16,518
	10 20 30 40 50 60 METGALRRPQ LLPLLLLLCG GCPRAGGCNE TGMLERLPLC GKAFADMMGK VDVWKWCNLS 70 80 90 100 110 120 EFIVYYESFT NCTEMEANVV GCYWPNPLAQ GFITGIHRQF FSNCTVDRVH LEDPPDEVLI 130 140 PLIVIPVVLT VAMAGLVVWR SKRTDTLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor." McLatchie L.M., Fraser N.J., Main M.J., Wise A., Brown J., Thompson N., Solari R., Lee M.G., Foord S.M. Nature 393:333-339(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Brain.
[2]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-33. Tissue: Lung and PNS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ001016 mRNA. Translation: CAA04474.1. AY265459 mRNA. Translation: AAP23300.1. AC004844 Genomic DNA. No translation available. BC022304 mRNA. Translation: AAH22304.1. BC053852 mRNA. Translation: AAH53852.1.
IPI	IPI00032425.
RefSeq	NP_005847.1. NM_005856.2.
UniGene	Hs.25691.
3D structure databases
ProteinModelPortal	O60896.
ModBase	Search...
Protein-protein interaction databases
IntAct	O60896. 2 interactions.
MINT	MINT-1384016.
STRING	9606.ENSP00000242249.
Proteomic databases
PaxDb	O60896.
PRIDE	O60896.
Protocols and materials databases
DNASU	10268.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000242249; ENSP00000242249; ENSG00000122679. ENST00000481345; ENSP00000419012; ENSG00000122679.
GeneID	10268.
KEGG	hsa:10268.
UCSC	uc003tnb.3. human.
Organism-specific databases
CTD	10268.
GeneCards	GC07P045163.
HGNC	HGNC:9845. RAMP3.
MIM	605155. gene.
neXtProt	NX_O60896.
PharmGKB	PA34204.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG39494.
HOGENOM	HOG000253018.
HOVERGEN	HBG061268.
InParanoid	O60896.
KO	K08449.
OrthoDB	EOG4K0QPX.
PhylomeDB	O60896.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	O60896.
Bgee	O60896.
CleanEx	HS_RAMP3.
Genevestigator	O60896.
GermOnline	ENSG00000122679. Homo sapiens.
Family and domain databases
InterPro	IPR006985. RAMP. [Graphical view]
PANTHER	PTHR14076. PTHR14076. 1 hit.
Pfam	PF04901. RAMP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB01278. Pramlintide.
GenomeRNAi	10268.
NextBio	38902.
SOURCE	Search...

Entry information

Entry name

RAMP3_HUMAN

Accession

Primary (citable) accession number: O60896
Secondary accession number(s): Q7Z2Y1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.