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O60896 (RAMP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Receptor activity-modifying protein 3
Alternative name(s):
Calcitonin-receptor-like receptor activity-modifying protein 3
Short name=CRLR activity-modifying protein 3
Gene names
Name:RAMP3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length148 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a GPER1-dependent manner. Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) and GPER1 to the plasma membrane. Acts as a receptor for adrenomedullin (AM) together with CALCRL. Ref.1 Ref.5

Subunit structure

Heterodimer of CALCRL and RAMP3 By similarity. Interacts with GPER1. Ref.5

Subcellular location

Cell membrane; Single-pass type I membrane protein. Membrane; Single-pass type I membrane protein. Note: Moves from intracellular puncta to the plasma membrane in a RAMP3-dependent manner. Ref.5

Tissue specificity

Strongly expressed in lung, breast, immune system and fetal tissues. Ref.1

Sequence similarities

Belongs to the RAMP family.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway involved in heart process

Inferred from sequence or structural similarity. Source: UniProtKB

calcium ion transport

Inferred from direct assay PubMed 10882736. Source: UniProtKB

cellular response to estradiol stimulus

Inferred from sequence or structural similarity. Source: UniProtKB

intracellular protein transport

Inferred from electronic annotation. Source: InterPro

negative regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 14722252. Source: UniProtKB

positive regulation of establishment of protein localization to plasma membrane

Inferred from direct assay Ref.5. Source: UniProtKB

positive regulation of receptor recycling

Inferred from direct assay PubMed 15613468. Source: UniProtKB

protein localization to plasma membrane

Inferred from direct assay PubMed 10882736. Source: UniProtKB

protein transport

Inferred from direct assay PubMed 10882736Ref.1. Source: UniProtKB

receptor internalization

Inferred from direct assay PubMed 10882736PubMed 15613468. Source: UniProtKB

regulation of G-protein coupled receptor protein signaling pathway

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcell surface

Inferred from direct assay PubMed 10882736PubMed 14722252. Source: UniProtKB

integral component of plasma membrane

Traceable author statement Ref.1. Source: ProtInc

intracellular

Inferred from direct assay Ref.5. Source: UniProtKB

lysosome

Traceable author statement PubMed 10882736. Source: ProtInc

plasma membrane

Inferred from direct assay PubMed 15613468Ref.5. Source: UniProtKB

receptor complex

Inferred from direct assay PubMed 15613468. Source: UniProtKB

   Molecular_functioncoreceptor activity

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 15613468Ref.5. Source: UniProtKB

protein transporter activity

Inferred from direct assay PubMed 10882736Ref.1. Source: UniProtKB

receptor activity

Inferred from direct assay PubMed 10882736. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 148125Receptor activity-modifying protein 3
PRO_0000030176

Regions

Topological domain24 – 11895Extracellular Potential
Transmembrane119 – 13820Helical; Potential
Topological domain139 – 14810Cytoplasmic Potential

Amino acid modifications

Glycosylation291N-linked (GlcNAc...) Potential
Glycosylation581N-linked (GlcNAc...) Potential
Glycosylation711N-linked (GlcNAc...) Potential
Glycosylation1031N-linked (GlcNAc...) Potential
Disulfide bond40 ↔ 72 By similarity
Disulfide bond57 ↔ 104 By similarity

Natural variations

Natural variant261G → D.
Corresponds to variant rs10272187 [ dbSNP | Ensembl ].
VAR_034437
Natural variant331M → L. Ref.4
Corresponds to variant rs11550711 [ dbSNP | Ensembl ].
VAR_053628
Natural variant561W → R.
Corresponds to variant rs2074654 [ dbSNP | Ensembl ].
VAR_024602

Sequences

Sequence LengthMass (Da)Tools
O60896 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: EEEE312496EF513C

FASTA14816,518
        10         20         30         40         50         60 
METGALRRPQ LLPLLLLLCG GCPRAGGCNE TGMLERLPLC GKAFADMMGK VDVWKWCNLS 

        70         80         90        100        110        120 
EFIVYYESFT NCTEMEANVV GCYWPNPLAQ GFITGIHRQF FSNCTVDRVH LEDPPDEVLI 

       130        140 
PLIVIPVVLT VAMAGLVVWR SKRTDTLL 

« Hide

References

« Hide 'large scale' references
[1]"RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor."
McLatchie L.M., Fraser N.J., Main M.J., Wise A., Brown J., Thompson N., Solari R., Lee M.G., Foord S.M.
Nature 393:333-339(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Tissue: Brain.
[2]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Kopatz S.A., Aronstam R.S., Sharma S.V.
Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-33.
Tissue: Lung and PNS.
[5]"G-protein-coupled receptor 30 interacts with receptor activity-modifying protein 3 and confers sex-dependent cardioprotection."
Lenhart P.M., Broselid S., Barrick C.J., Leeb-Lundberg L.M., Caron K.M.
J. Mol. Endocrinol. 51:191-202(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH GPER1, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ001016 mRNA. Translation: CAA04474.1.
AY265459 mRNA. Translation: AAP23300.1.
AC004844 Genomic DNA. No translation available.
BC022304 mRNA. Translation: AAH22304.1.
BC053852 mRNA. Translation: AAH53852.1.
CCDSCCDS5503.1.
RefSeqNP_005847.1. NM_005856.2.
UniGeneHs.25691.

3D structure databases

ProteinModelPortalO60896.
SMRO60896. Positions 39-104.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115559. 4 interactions.
IntActO60896. 2 interactions.
MINTMINT-1384016.
STRING9606.ENSP00000242249.

Chemistry

ChEMBLCHEMBL2111191.
DrugBankDB01278. Pramlintide.

Proteomic databases

PaxDbO60896.
PRIDEO60896.

Protocols and materials databases

DNASU10268.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000242249; ENSP00000242249; ENSG00000122679.
ENST00000481345; ENSP00000419012; ENSG00000122679.
GeneID10268.
KEGGhsa:10268.
UCSCuc003tnb.3. human.

Organism-specific databases

CTD10268.
GeneCardsGC07P045163.
HGNCHGNC:9845. RAMP3.
MIM605155. gene.
neXtProtNX_O60896.
PharmGKBPA34204.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG39494.
HOGENOMHOG000253018.
HOVERGENHBG061268.
InParanoidO60896.
KOK08449.
OrthoDBEOG7ZKSD3.
PhylomeDBO60896.
TreeFamTF333286.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressO60896.
BgeeO60896.
CleanExHS_RAMP3.
GenevestigatorO60896.

Family and domain databases

InterProIPR006985. RAMP.
[Graphical view]
PANTHERPTHR14076. PTHR14076. 1 hit.
PfamPF04901. RAMP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiRAMP3.
GenomeRNAi10268.
NextBio38902.
PROO60896.
SOURCESearch...

Entry information

Entry nameRAMP3_HUMAN
AccessionPrimary (citable) accession number: O60896
Secondary accession number(s): Q7Z2Y1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM