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Protein

Receptor activity-modifying protein 2

Gene

RAMP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for adrenomedullin (AM) together with CALCRL.2 Publications

GO - Molecular functioni

  • adrenomedullin binding Source: UniProtKB
  • adrenomedullin receptor activity Source: UniProtKB
  • coreceptor activity Source: UniProtKB
  • protein transporter activity Source: UniProtKB

GO - Biological processi

  • adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: UniProtKB
  • adherens junction assembly Source: UniProtKB
  • adrenomedullin receptor signaling pathway Source: UniProtKB
  • amylin receptor signaling pathway Source: ARUK-UCL
  • angiogenesis Source: UniProtKB
  • basement membrane assembly Source: UniProtKB
  • bicellular tight junction assembly Source: UniProtKB
  • calcium ion transport Source: UniProtKB
  • cellular response to hormone stimulus Source: Ensembl
  • cellular response to vascular endothelial growth factor stimulus Source: UniProtKB
  • female pregnancy Source: Ensembl
  • G-protein coupled receptor signaling pathway Source: Reactome
  • heart development Source: UniProtKB
  • intracellular protein transport Source: InterPro
  • negative regulation of endothelial cell apoptotic process Source: UniProtKB
  • negative regulation of vascular permeability Source: UniProtKB
  • positive regulation of angiogenesis Source: UniProtKB
  • positive regulation of cAMP metabolic process Source: ARUK-UCL
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of vasculogenesis Source: Ensembl
  • protein localization to plasma membrane Source: UniProtKB
  • protein transport Source: UniProtKB
  • receptor internalization Source: UniProtKB
  • regulation of blood pressure Source: UniProtKB
  • regulation of G-protein coupled receptor protein signaling pathway Source: InterPro
  • response to estradiol Source: Ensembl
  • response to hypoxia Source: Ensembl
  • response to progesterone Source: Ensembl
  • sprouting angiogenesis Source: UniProtKB
  • vascular smooth muscle cell development Source: UniProtKB
  • vasculogenesis Source: UniProtKB

Keywordsi

Molecular functionReceptor
Biological processTransport

Enzyme and pathway databases

ReactomeiR-HSA-418555 G alpha (s) signalling events
R-HSA-419812 Calcitonin-like ligand receptors

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor activity-modifying protein 2
Alternative name(s):
Calcitonin-receptor-like receptor activity-modifying protein 2
Short name:
CRLR activity-modifying protein 2
Gene namesi
Name:RAMP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000131477.10
HGNCiHGNC:9844 RAMP2
MIMi605154 gene
neXtProtiNX_O60895

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini43 – 145ExtracellularSequence analysisAdd BLAST103
Transmembranei146 – 166HelicalSequence analysisAdd BLAST21
Topological domaini167 – 175CytoplasmicSequence analysis9

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10266
OpenTargetsiENSG00000131477
PharmGKBiPA34203

Chemistry databases

ChEMBLiCHEMBL2364173
DrugBankiDB01278 Pramlintide
GuidetoPHARMACOLOGYi52

Polymorphism and mutation databases

BioMutaiRAMP2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 42Sequence analysisAdd BLAST42
ChainiPRO_000003017243 – 175Receptor activity-modifying protein 2Add BLAST133

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi68 ↔ 99
Disulfide bondi84 ↔ 131
Glycosylationi130N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO60895
PeptideAtlasiO60895
PRIDEiO60895

PTM databases

PhosphoSitePlusiO60895

Expressioni

Tissue specificityi

Strongly expressed in lung, breast, immune system and fetal tissues.1 Publication

Gene expression databases

BgeeiENSG00000131477
CleanExiHS_RAMP2
ExpressionAtlasiO60895 baseline and differential
GenevisibleiO60895 HS

Organism-specific databases

HPAiHPA052020

Interactioni

Subunit structurei

Heterodimer of CALCRL and RAMP2.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CALCRLQ166026EBI-9009040,EBI-962878

Protein-protein interaction databases

BioGridi115557, 1 interactor
CORUMiO60895
IntActiO60895, 2 interactors
STRINGi9606.ENSP00000253796

Chemistry databases

BindingDBiO60895

Structurei

Secondary structure

1175
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi49 – 54Combined sources6
Helixi61 – 76Combined sources16
Helixi77 – 82Combined sources6
Helixi86 – 106Combined sources21
Helixi114 – 126Combined sources13
Turni127 – 130Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XVTX-ray2.05A/B/C/D/E/F48-139[»]
3AQEX-ray2.00A/B/C/D/E/F56-139[»]
3AQFX-ray2.60A56-139[»]
4RWFX-ray1.76A55-138[»]
ProteinModelPortaliO60895
SMRiO60895
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RAMP family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYPR Eukaryota
ENOG4112A9G LUCA
GeneTreeiENSGT00390000016200
HOGENOMiHOG000230963
HOVERGENiHBG067366
InParanoidiO60895
KOiK08448
OMAiDLGFPNP
OrthoDBiEOG091G0RMZ
PhylomeDBiO60895
TreeFamiTF333286

Family and domain databases

Gene3Di1.10.150.510, 1 hit
InterProiView protein in InterPro
IPR006985 RAMP
IPR038126 RAMP_sf
PANTHERiPTHR14076 PTHR14076, 1 hit
PfamiView protein in Pfam
PF04901 RAMP, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O60895-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASLRVERAG GPRLPRTRVG RPAALRLLLL LGAVLNPHEA LAQPLPTTGT
60 70 80 90 100
PGSEGGTVKN YETAVQFCWN HYKDQMDPIE KDWCDWAMIS RPYSTLRDCL
110 120 130 140 150
EHFAELFDLG FPNPLAERII FETHQIHFAN CSLVQPTFSD PPEDVLLAMI
160 170
IAPICLIPFL ITLVVWRSKD SEAQA
Length:175
Mass (Da):19,608
Last modified:January 10, 2003 - v2
Checksum:iAF69A9A461EFFCA3
GO
Isoform 2 (identifier: O60895-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-54: E → EASVPT

Show »
Length:180
Mass (Da):20,063
Checksum:i17FEBC82A10A0E2F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13R → C in ABS28868 (Ref. 2) Curated1
Sequence conflicti25L → V in CAA04473 (PubMed:9620797).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05583854E → EASVPT in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001015 mRNA Translation: CAA04473.1
EF687002 mRNA Translation: ABS28868.1
AY265458 mRNA Translation: AAP23299.1
AC100793 Genomic DNA No translation available.
BC027975 mRNA Translation: AAH27975.1
CCDSiCCDS11437.1 [O60895-1]
RefSeqiNP_005845.2, NM_005854.2 [O60895-1]
UniGeneiHs.514193

Genome annotation databases

EnsembliENST00000253796; ENSP00000253796; ENSG00000131477 [O60895-1]
ENST00000587142; ENSP00000466455; ENSG00000131477 [O60895-2]
GeneIDi10266
KEGGihsa:10266
UCSCiuc002ibg.5 human [O60895-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRAMP2_HUMAN
AccessioniPrimary (citable) accession number: O60895
Secondary accession number(s): A7L9S6, K7EMD3, Q8N1F2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 10, 2003
Last modified: March 28, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health