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O60895

- RAMP2_HUMAN

UniProt

O60895 - RAMP2_HUMAN

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Protein

Receptor activity-modifying protein 2

Gene

RAMP2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for adrenomedullin (AM) together with CALCRL.2 Publications

GO - Molecular functioni

  1. coreceptor activity Source: UniProtKB
  2. protein transporter activity Source: UniProtKB

GO - Biological processi

  1. adherens junction assembly Source: UniProtKB
  2. angiogenesis Source: UniProtKB
  3. basement membrane assembly Source: UniProtKB
  4. calcium ion transport Source: UniProtKB
  5. cAMP biosynthetic process Source: UniProtKB
  6. cellular response to hormone stimulus Source: Ensembl
  7. cellular response to vascular endothelial growth factor stimulus Source: UniProtKB
  8. female pregnancy Source: Ensembl
  9. G-protein coupled receptor signaling pathway Source: Ensembl
  10. heart development Source: UniProtKB
  11. intracellular protein transport Source: InterPro
  12. negative regulation of endothelial cell apoptotic process Source: UniProtKB
  13. negative regulation of vascular permeability Source: UniProtKB
  14. positive regulation of angiogenesis Source: UniProtKB
  15. positive regulation of cAMP biosynthetic process Source: UniProtKB
  16. positive regulation of gene expression Source: UniProtKB
  17. positive regulation of vasculogenesis Source: Ensembl
  18. protein localization to plasma membrane Source: UniProtKB
  19. protein transport Source: UniProtKB
  20. receptor internalization Source: UniProtKB
  21. regulation of blood pressure Source: UniProtKB
  22. regulation of G-protein coupled receptor protein signaling pathway Source: InterPro
  23. response to estradiol Source: Ensembl
  24. response to hypoxia Source: Ensembl
  25. response to progesterone Source: Ensembl
  26. sprouting angiogenesis Source: UniProtKB
  27. tight junction assembly Source: UniProtKB
  28. vascular smooth muscle cell development Source: UniProtKB
  29. vasculogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_18290. Calcitonin-like ligand receptors.
REACT_19327. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor activity-modifying protein 2
Alternative name(s):
Calcitonin-receptor-like receptor activity-modifying protein 2
Short name:
CRLR activity-modifying protein 2
Gene namesi
Name:RAMP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:9844. RAMP2.

Subcellular locationi

GO - Cellular componenti

  1. cell surface Source: UniProtKB
  2. coated pit Source: ProtInc
  3. cytoplasm Source: UniProtKB
  4. integral component of plasma membrane Source: ProtInc
  5. lysosome Source: ProtInc
  6. plasma membrane Source: Reactome
  7. receptor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34203.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4242Sequence AnalysisAdd
BLAST
Chaini43 – 175133Receptor activity-modifying protein 2PRO_0000030172Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi68 ↔ 99
Disulfide bondi84 ↔ 131
Glycosylationi130 – 1301N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO60895.
PRIDEiO60895.

PTM databases

PhosphoSiteiO60895.

Expressioni

Tissue specificityi

Strongly expressed in lung, breast, immune system and fetal tissues.1 Publication

Gene expression databases

BgeeiO60895.
CleanExiHS_RAMP2.
ExpressionAtlasiO60895. baseline and differential.
GenevestigatoriO60895.

Interactioni

Subunit structurei

Heterodimer of CALCRL and RAMP2.By similarity

Protein-protein interaction databases

BioGridi115557. 1 interaction.
STRINGi9606.ENSP00000253796.

Structurei

Secondary structure

1
175
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi61 – 7616
Helixi77 – 826
Helixi86 – 10621
Helixi114 – 12613
Turni127 – 1304

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XVTX-ray2.05A/B/C/D/E/F48-139[»]
3AQEX-ray2.00A/B/C/D/E/F56-139[»]
3AQFX-ray2.60A56-139[»]
ProteinModelPortaliO60895.
SMRiO60895. Positions 60-135.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini43 – 145103ExtracellularSequence AnalysisAdd
BLAST
Topological domaini167 – 1759CytoplasmicSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei146 – 16621HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the RAMP family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG42995.
GeneTreeiENSGT00390000016200.
HOGENOMiHOG000230963.
HOVERGENiHBG067366.
InParanoidiO60895.
KOiK08448.
PhylomeDBiO60895.
TreeFamiTF333286.

Family and domain databases

InterProiIPR006985. RAMP.
[Graphical view]
PANTHERiPTHR14076. PTHR14076. 1 hit.
PfamiPF04901. RAMP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O60895-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASLRVERAG GPRLPRTRVG RPAALRLLLL LGAVLNPHEA LAQPLPTTGT
60 70 80 90 100
PGSEGGTVKN YETAVQFCWN HYKDQMDPIE KDWCDWAMIS RPYSTLRDCL
110 120 130 140 150
EHFAELFDLG FPNPLAERII FETHQIHFAN CSLVQPTFSD PPEDVLLAMI
160 170
IAPICLIPFL ITLVVWRSKD SEAQA
Length:175
Mass (Da):19,608
Last modified:January 10, 2003 - v2
Checksum:iAF69A9A461EFFCA3
GO
Isoform 2 (identifier: O60895-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-54: E → EASVPT

Show »
Length:180
Mass (Da):20,063
Checksum:i17FEBC82A10A0E2F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131R → C in ABS28868. 1 PublicationCurated
Sequence conflicti25 – 251L → V in CAA04473. (PubMed:9620797)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei54 – 541E → EASVPT in isoform 2. 1 PublicationVSP_055838

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ001015 mRNA. Translation: CAA04473.1.
EF687002 mRNA. Translation: ABS28868.1.
AY265458 mRNA. Translation: AAP23299.1.
AC100793 Genomic DNA. No translation available.
BC027975 mRNA. Translation: AAH27975.1.
CCDSiCCDS11437.1. [O60895-1]
RefSeqiNP_005845.2. NM_005854.2. [O60895-1]
UniGeneiHs.514193.

Genome annotation databases

EnsembliENST00000253796; ENSP00000253796; ENSG00000131477. [O60895-1]
ENST00000587142; ENSP00000466455; ENSG00000131477. [O60895-2]
GeneIDi10266.
KEGGihsa:10266.
UCSCiuc002ibg.3. human. [O60895-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ001015 mRNA. Translation: CAA04473.1 .
EF687002 mRNA. Translation: ABS28868.1 .
AY265458 mRNA. Translation: AAP23299.1 .
AC100793 Genomic DNA. No translation available.
BC027975 mRNA. Translation: AAH27975.1 .
CCDSi CCDS11437.1. [O60895-1 ]
RefSeqi NP_005845.2. NM_005854.2. [O60895-1 ]
UniGenei Hs.514193.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2XVT X-ray 2.05 A/B/C/D/E/F 48-139 [» ]
3AQE X-ray 2.00 A/B/C/D/E/F 56-139 [» ]
3AQF X-ray 2.60 A 56-139 [» ]
ProteinModelPortali O60895.
SMRi O60895. Positions 60-135.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115557. 1 interaction.
STRINGi 9606.ENSP00000253796.

Chemistry

ChEMBLi CHEMBL2109232.
DrugBanki DB01278. Pramlintide.

PTM databases

PhosphoSitei O60895.

Proteomic databases

PaxDbi O60895.
PRIDEi O60895.

Protocols and materials databases

DNASUi 10266.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000253796 ; ENSP00000253796 ; ENSG00000131477 . [O60895-1 ]
ENST00000587142 ; ENSP00000466455 ; ENSG00000131477 . [O60895-2 ]
GeneIDi 10266.
KEGGi hsa:10266.
UCSCi uc002ibg.3. human. [O60895-1 ]

Organism-specific databases

CTDi 10266.
GeneCardsi GC17P040913.
HGNCi HGNC:9844. RAMP2.
MIMi 605154. gene.
neXtProti NX_O60895.
PharmGKBi PA34203.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG42995.
GeneTreei ENSGT00390000016200.
HOGENOMi HOG000230963.
HOVERGENi HBG067366.
InParanoidi O60895.
KOi K08448.
PhylomeDBi O60895.
TreeFami TF333286.

Enzyme and pathway databases

Reactomei REACT_18290. Calcitonin-like ligand receptors.
REACT_19327. G alpha (s) signalling events.

Miscellaneous databases

GeneWikii RAMP2.
GenomeRNAii 10266.
NextBioi 35463350.
PROi O60895.
SOURCEi Search...

Gene expression databases

Bgeei O60895.
CleanExi HS_RAMP2.
ExpressionAtlasi O60895. baseline and differential.
Genevestigatori O60895.

Family and domain databases

InterProi IPR006985. RAMP.
[Graphical view ]
PANTHERi PTHR14076. PTHR14076. 1 hit.
Pfami PF04901. RAMP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor."
    McLatchie L.M., Fraser N.J., Main M.J., Wise A., Brown J., Thompson N., Solari R., Lee M.G., Foord S.M.
    Nature 393:333-339(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Neuroblastoma.
  2. "Characterization of adrenomedullin receptors and identification of a receptor activity modifying protein 2 (RAMP2) variant in SV40LT-SMC cells."
    Rorabaugh B.R., Witt K.M., Smith D.D., Abel P.W., Scofield M.A.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  3. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Kopatz S.A., Aronstam R.S., Sharma S.V.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Heart.
  4. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Pancreas.
  6. "Structure of the extracellular domain of human RAMP2."
    Structural genomics consortium (SGC)
    Submitted (DEC-2010) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 48-139, DISULFIDE BONDS.
  7. "Structural basis for extracellular interactions between calcitonin receptor-like receptor and receptor activity-modifying protein 2 for adrenomedullin-specific binding."
    Kusano S., Kukimoto-Niino M., Hino N., Ohsawa N., Okuda K., Sakamoto K., Shirouzu M., Shindo T., Yokoyama S.
    Protein Sci. 21:199-210(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 52-139 IN COMPLEX WITH CALCRL, FUNCTION, DISULFIDE BONDS.

Entry informationi

Entry nameiRAMP2_HUMAN
AccessioniPrimary (citable) accession number: O60895
Secondary accession number(s): A7L9S6, K7EMD3, Q8N1F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 10, 2003
Last modified: October 29, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3