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O60894

- RAMP1_HUMAN

UniProt

O60894 - RAMP1_HUMAN

Protein

Receptor activity-modifying protein 1

Gene

RAMP1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for calcitonin-gene-related peptide (CGRP) together with CALCRL.1 Publication

    GO - Molecular functioni

    1. calcitonin receptor activity Source: UniProtKB
    2. calcitonin receptor binding Source: UniProtKB
    3. coreceptor activity Source: Ensembl
    4. protein binding Source: UniProtKB
    5. protein transporter activity Source: UniProtKB
    6. receptor activity Source: UniProtKB

    GO - Biological processi

    1. angiogenesis Source: UniProtKB
    2. calcium ion transport Source: UniProtKB
    3. intracellular protein transport Source: InterPro
    4. positive regulation of cAMP biosynthetic process Source: UniProtKB
    5. positive regulation of protein glycosylation Source: UniProtKB
    6. protein localization to plasma membrane Source: UniProtKB
    7. protein transport Source: UniProtKB
    8. receptor internalization Source: UniProtKB
    9. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    ReactomeiREACT_18290. Calcitonin-like ligand receptors.
    REACT_19327. G alpha (s) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Receptor activity-modifying protein 1
    Alternative name(s):
    Calcitonin-receptor-like receptor activity-modifying protein 1
    Short name:
    CRLR activity-modifying protein 1
    Gene namesi
    Name:RAMP1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:9843. RAMP1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell surface Source: UniProtKB
    2. extracellular space Source: Ensembl
    3. integral component of plasma membrane Source: ProtInc
    4. plasma membrane Source: BHF-UCL
    5. receptor complex Source: UniProtKB

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34202.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 148122Receptor activity-modifying protein 1PRO_0000030168Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi27 ↔ 82
    Disulfide bondi40 ↔ 72
    Disulfide bondi57 ↔ 104

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiO60894.
    PRIDEiO60894.

    Expressioni

    Tissue specificityi

    Expressed in many tissues including the uterus, bladder, brain, pancreas and gastro-intestinal tract.1 Publication

    Gene expression databases

    ArrayExpressiO60894.
    BgeeiO60894.
    CleanExiHS_RAMP1.
    GenevestigatoriO60894.

    Organism-specific databases

    HPAiHPA057814.

    Interactioni

    Subunit structurei

    Heterodimer of CALCRL and RAMP1.2 Publications

    Protein-protein interaction databases

    BioGridi115558. 3 interactions.
    DIPiDIP-37675N.
    IntActiO60894. 1 interaction.
    STRINGi9606.ENSP00000254661.

    Structurei

    Secondary structure

    1
    148
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi29 – 3911
    Helixi41 – 5111
    Helixi53 – 553
    Helixi59 – 8022
    Helixi87 – 10014
    Beta strandi101 – 1033
    Beta strandi111 – 1133

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2YX8X-ray2.40A27-112[»]
    3N7PX-ray2.80D/E/F/R26-117[»]
    3N7RX-ray2.90C/D26-117[»]
    3N7SX-ray2.10C/D26-117[»]
    ProteinModelPortaliO60894.
    SMRiO60894. Positions 26-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO60894.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini27 – 11791ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini139 – 14810CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei118 – 13821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RAMP family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG41532.
    HOGENOMiHOG000253018.
    HOVERGENiHBG061268.
    InParanoidiO60894.
    KOiK08447.
    OMAiPNRLVDE.
    OrthoDBiEOG7ZKSD3.
    PhylomeDBiO60894.
    TreeFamiTF333286.

    Family and domain databases

    InterProiIPR006985. RAMP.
    [Graphical view]
    PANTHERiPTHR14076. PTHR14076. 1 hit.
    PfamiPF04901. RAMP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O60894-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARALCRLPR RGLWLLLAHH LFMTTACQEA NYGALLRELC LTQFQVDMEA    50
    VGETLWCDWG RTIRSYRELA DCTWHMAEKL GCFWPNAEVD RFFLAVHGRY 100
    FRSCPISGRA VRDPPGSILY PFIVVPITVT LLVTALVVWQ SKRTEGIV 148
    Length:148
    Mass (Da):16,988
    Last modified:August 1, 1998 - v1
    Checksum:i8530DD590BAEBE5C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001014 mRNA. Translation: CAA04472.1.
    AY265457 mRNA. Translation: AAP23298.1.
    CR542032 mRNA. Translation: CAG46829.1.
    CR542044 mRNA. Translation: CAG46841.1.
    CH471063 Genomic DNA. Translation: EAW71127.1.
    BC000548 mRNA. Translation: AAH00548.1.
    CCDSiCCDS2522.1.
    RefSeqiNP_005846.1. NM_005855.2.
    UniGeneiHs.471783.

    Genome annotation databases

    EnsembliENST00000254661; ENSP00000254661; ENSG00000132329.
    GeneIDi10267.
    KEGGihsa:10267.
    UCSCiuc002vxj.3. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001014 mRNA. Translation: CAA04472.1 .
    AY265457 mRNA. Translation: AAP23298.1 .
    CR542032 mRNA. Translation: CAG46829.1 .
    CR542044 mRNA. Translation: CAG46841.1 .
    CH471063 Genomic DNA. Translation: EAW71127.1 .
    BC000548 mRNA. Translation: AAH00548.1 .
    CCDSi CCDS2522.1.
    RefSeqi NP_005846.1. NM_005855.2.
    UniGenei Hs.471783.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2YX8 X-ray 2.40 A 27-112 [» ]
    3N7P X-ray 2.80 D/E/F/R 26-117 [» ]
    3N7R X-ray 2.90 C/D 26-117 [» ]
    3N7S X-ray 2.10 C/D 26-117 [» ]
    ProteinModelPortali O60894.
    SMRi O60894. Positions 26-116.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115558. 3 interactions.
    DIPi DIP-37675N.
    IntActi O60894. 1 interaction.
    STRINGi 9606.ENSP00000254661.

    Chemistry

    BindingDBi O60894.
    ChEMBLi CHEMBL2107838.
    DrugBanki DB01278. Pramlintide.

    Proteomic databases

    PaxDbi O60894.
    PRIDEi O60894.

    Protocols and materials databases

    DNASUi 10267.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000254661 ; ENSP00000254661 ; ENSG00000132329 .
    GeneIDi 10267.
    KEGGi hsa:10267.
    UCSCi uc002vxj.3. human.

    Organism-specific databases

    CTDi 10267.
    GeneCardsi GC02P238767.
    HGNCi HGNC:9843. RAMP1.
    HPAi HPA057814.
    MIMi 605153. gene.
    neXtProti NX_O60894.
    PharmGKBi PA34202.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG41532.
    HOGENOMi HOG000253018.
    HOVERGENi HBG061268.
    InParanoidi O60894.
    KOi K08447.
    OMAi PNRLVDE.
    OrthoDBi EOG7ZKSD3.
    PhylomeDBi O60894.
    TreeFami TF333286.

    Enzyme and pathway databases

    Reactomei REACT_18290. Calcitonin-like ligand receptors.
    REACT_19327. G alpha (s) signalling events.

    Miscellaneous databases

    ChiTaRSi RAMP1. human.
    EvolutionaryTracei O60894.
    GeneWikii RAMP1.
    GenomeRNAii 10267.
    NextBioi 38898.
    PROi O60894.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O60894.
    Bgeei O60894.
    CleanExi HS_RAMP1.
    Genevestigatori O60894.

    Family and domain databases

    InterProi IPR006985. RAMP.
    [Graphical view ]
    PANTHERi PTHR14076. PTHR14076. 1 hit.
    Pfami PF04901. RAMP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor."
      McLatchie L.M., Fraser N.J., Main M.J., Wise A., Brown J., Thompson N., Solari R., Lee M.G., Foord S.M.
      Nature 393:333-339(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, TOPOLOGY.
      Tissue: Neuroblastoma.
    2. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Kopatz S.A., Aronstam R.S., Sharma S.V.
      Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    6. "Crystal structure of the human receptor activity-modifying protein 1 extracellular domain."
      Kusano S., Kukimoto-Niino M., Akasaka R., Toyama M., Terada T., Shirouzu M., Shindo T., Yokoyama S.
      Protein Sci. 17:1907-1914(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 22-112, SUBUNIT, DISULFIDE BONDS.
    7. "Crystal structure of the ectodomain complex of the CGRP receptor, a class-B GPCR, reveals the site of drug antagonism."
      ter Haar E., Koth C.M., Abdul-Manan N., Swenson L., Coll J.T., Lippke J.A., Lepre C.A., Garcia-Guzman M., Moore J.M.
      Structure 18:1083-1093(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 26-117 IN COMPLEX WITH CALRL AND ANTAGONIST, SUBUNIT, DISULFIDE BONDS.

    Entry informationi

    Entry nameiRAMP1_HUMAN
    AccessioniPrimary (citable) accession number: O60894
    Secondary accession number(s): Q6FGS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3