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Protein

Receptor activity-modifying protein 1

Gene

RAMP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for calcitonin-gene-related peptide (CGRP) together with CALCRL.1 Publication

GO - Molecular functioni

  • calcitonin receptor activity Source: UniProtKB
  • calcitonin receptor binding Source: UniProtKB
  • coreceptor activity Source: Ensembl
  • protein transporter activity Source: UniProtKB
  • receptor activity Source: UniProtKB

GO - Biological processi

  • angiogenesis Source: UniProtKB
  • calcium ion transport Source: UniProtKB
  • intracellular protein transport Source: InterPro
  • positive regulation of cAMP biosynthetic process Source: UniProtKB
  • positive regulation of protein glycosylation Source: UniProtKB
  • protein localization to plasma membrane Source: UniProtKB
  • protein transport Source: UniProtKB
  • receptor internalization Source: UniProtKB
  • regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_18290. Calcitonin-like ligand receptors.
REACT_19327. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor activity-modifying protein 1
Alternative name(s):
Calcitonin-receptor-like receptor activity-modifying protein 1
Short name:
CRLR activity-modifying protein 1
Gene namesi
Name:RAMP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:9843. RAMP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 11791ExtracellularSequence AnalysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence AnalysisAdd
BLAST
Topological domaini139 – 14810CytoplasmicSequence Analysis

GO - Cellular componenti

  • cell surface Source: UniProtKB
  • extracellular space Source: Ensembl
  • integral component of plasma membrane Source: ProtInc
  • intracellular Source: GOC
  • plasma membrane Source: BHF-UCL
  • receptor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34202.

Chemistry

DrugBankiDB01278. Pramlintide.

Polymorphism and mutation databases

BioMutaiRAMP1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 148122Receptor activity-modifying protein 1PRO_0000030168Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 82
Disulfide bondi40 ↔ 72
Disulfide bondi57 ↔ 104

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO60894.
PRIDEiO60894.

Expressioni

Tissue specificityi

Expressed in many tissues including the uterus, bladder, brain, pancreas and gastro-intestinal tract.1 Publication

Gene expression databases

BgeeiO60894.
CleanExiHS_RAMP1.
ExpressionAtlasiO60894. baseline and differential.
GenevisibleiO60894. HS.

Interactioni

Subunit structurei

Heterodimer of CALCRL and RAMP1.2 Publications

Protein-protein interaction databases

BioGridi115558. 3 interactions.
DIPiDIP-37675N.
IntActiO60894. 1 interaction.
STRINGi9606.ENSP00000254661.

Structurei

Secondary structure

1
148
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 3911Combined sources
Helixi41 – 5111Combined sources
Helixi53 – 553Combined sources
Helixi59 – 8022Combined sources
Helixi87 – 10014Combined sources
Beta strandi101 – 1033Combined sources
Beta strandi111 – 1133Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YX8X-ray2.40A27-112[»]
3N7PX-ray2.80D/E/F/R26-117[»]
3N7RX-ray2.90C/D26-117[»]
3N7SX-ray2.10C/D26-117[»]
ProteinModelPortaliO60894.
SMRiO60894. Positions 26-116.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60894.

Family & Domainsi

Sequence similaritiesi

Belongs to the RAMP family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG41532.
GeneTreeiENSGT00390000016200.
HOGENOMiHOG000253018.
HOVERGENiHBG061268.
InParanoidiO60894.
KOiK08447.
OMAiWRSKRPE.
OrthoDBiEOG7ZKSD3.
PhylomeDBiO60894.
TreeFamiTF333286.

Family and domain databases

InterProiIPR006985. RAMP.
[Graphical view]
PANTHERiPTHR14076. PTHR14076. 1 hit.
PfamiPF04901. RAMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O60894-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARALCRLPR RGLWLLLAHH LFMTTACQEA NYGALLRELC LTQFQVDMEA
60 70 80 90 100
VGETLWCDWG RTIRSYRELA DCTWHMAEKL GCFWPNAEVD RFFLAVHGRY
110 120 130 140
FRSCPISGRA VRDPPGSILY PFIVVPITVT LLVTALVVWQ SKRTEGIV
Length:148
Mass (Da):16,988
Last modified:August 1, 1998 - v1
Checksum:i8530DD590BAEBE5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001014 mRNA. Translation: CAA04472.1.
AY265457 mRNA. Translation: AAP23298.1.
CR542032 mRNA. Translation: CAG46829.1.
CR542044 mRNA. Translation: CAG46841.1.
CH471063 Genomic DNA. Translation: EAW71127.1.
BC000548 mRNA. Translation: AAH00548.1.
CCDSiCCDS2522.1.
RefSeqiNP_005846.1. NM_005855.3.
UniGeneiHs.471783.

Genome annotation databases

EnsembliENST00000254661; ENSP00000254661; ENSG00000132329.
GeneIDi10267.
KEGGihsa:10267.
UCSCiuc002vxj.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001014 mRNA. Translation: CAA04472.1.
AY265457 mRNA. Translation: AAP23298.1.
CR542032 mRNA. Translation: CAG46829.1.
CR542044 mRNA. Translation: CAG46841.1.
CH471063 Genomic DNA. Translation: EAW71127.1.
BC000548 mRNA. Translation: AAH00548.1.
CCDSiCCDS2522.1.
RefSeqiNP_005846.1. NM_005855.3.
UniGeneiHs.471783.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YX8X-ray2.40A27-112[»]
3N7PX-ray2.80D/E/F/R26-117[»]
3N7RX-ray2.90C/D26-117[»]
3N7SX-ray2.10C/D26-117[»]
ProteinModelPortaliO60894.
SMRiO60894. Positions 26-116.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115558. 3 interactions.
DIPiDIP-37675N.
IntActiO60894. 1 interaction.
STRINGi9606.ENSP00000254661.

Chemistry

BindingDBiO60894.
ChEMBLiCHEMBL2107838.
DrugBankiDB01278. Pramlintide.

Polymorphism and mutation databases

BioMutaiRAMP1.

Proteomic databases

PaxDbiO60894.
PRIDEiO60894.

Protocols and materials databases

DNASUi10267.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254661; ENSP00000254661; ENSG00000132329.
GeneIDi10267.
KEGGihsa:10267.
UCSCiuc002vxj.3. human.

Organism-specific databases

CTDi10267.
GeneCardsiGC02P238767.
HGNCiHGNC:9843. RAMP1.
MIMi605153. gene.
neXtProtiNX_O60894.
PharmGKBiPA34202.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG41532.
GeneTreeiENSGT00390000016200.
HOGENOMiHOG000253018.
HOVERGENiHBG061268.
InParanoidiO60894.
KOiK08447.
OMAiWRSKRPE.
OrthoDBiEOG7ZKSD3.
PhylomeDBiO60894.
TreeFamiTF333286.

Enzyme and pathway databases

ReactomeiREACT_18290. Calcitonin-like ligand receptors.
REACT_19327. G alpha (s) signalling events.

Miscellaneous databases

ChiTaRSiRAMP1. human.
EvolutionaryTraceiO60894.
GeneWikiiRAMP1.
GenomeRNAii10267.
NextBioi38898.
PROiO60894.
SOURCEiSearch...

Gene expression databases

BgeeiO60894.
CleanExiHS_RAMP1.
ExpressionAtlasiO60894. baseline and differential.
GenevisibleiO60894. HS.

Family and domain databases

InterProiIPR006985. RAMP.
[Graphical view]
PANTHERiPTHR14076. PTHR14076. 1 hit.
PfamiPF04901. RAMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor."
    McLatchie L.M., Fraser N.J., Main M.J., Wise A., Brown J., Thompson N., Solari R., Lee M.G., Foord S.M.
    Nature 393:333-339(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, TOPOLOGY.
    Tissue: Neuroblastoma.
  2. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Kopatz S.A., Aronstam R.S., Sharma S.V.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "Crystal structure of the human receptor activity-modifying protein 1 extracellular domain."
    Kusano S., Kukimoto-Niino M., Akasaka R., Toyama M., Terada T., Shirouzu M., Shindo T., Yokoyama S.
    Protein Sci. 17:1907-1914(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 22-112, SUBUNIT, DISULFIDE BONDS.
  7. "Crystal structure of the ectodomain complex of the CGRP receptor, a class-B GPCR, reveals the site of drug antagonism."
    ter Haar E., Koth C.M., Abdul-Manan N., Swenson L., Coll J.T., Lippke J.A., Lepre C.A., Garcia-Guzman M., Moore J.M.
    Structure 18:1083-1093(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 26-117 IN COMPLEX WITH CALRL AND ANTAGONIST, SUBUNIT, DISULFIDE BONDS.

Entry informationi

Entry nameiRAMP1_HUMAN
AccessioniPrimary (citable) accession number: O60894
Secondary accession number(s): Q6FGS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: June 24, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.