O60884 (DNJA2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DnaJ homolog subfamily A member 2

Alternative name(s):
Cell cycle progression restoration gene 3 protein
Dnj3
Short name=Dj3
HIRA-interacting protein 4
Renal carcinoma antigen NY-REN-14

Gene names

Name:	DNAJA2
Synonyms:	CPR3, HIRIP4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Co-chaperone of Hsc70.
Subcellular location	Membrane; Lipid-anchor Potential.
Sequence similarities	Contains 1 CR-type zinc finger. Contains 1 J domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Chaperone
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of cell proliferation Traceable author statement. Source: ProtInc protein folding Inferred from electronic annotation. Source: InterPro response to heat Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro heat shock protein binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	DnaJ homolog subfamily A member 2		PRO_0000071011
	Propeptide	410 – 412	3	Removed in mature form Probable		PRO_0000393942
Regions
	Domain	8 – 70	63	J
	Repeat	143 – 150	8	CXXCXGXG motif
	Repeat	159 – 166	8	CXXCXGXG motif
	Repeat	186 – 193	8	CXXCXGXG motif
	Repeat	202 – 209	8	CXXCXGXG motif
	Zinc finger	130 – 214	85	CR-type
Sites
	Metal binding	143	1	Zinc 1 By similarity
	Metal binding	146	1	Zinc 1 By similarity
	Metal binding	159	1	Zinc 2 By similarity
	Metal binding	162	1	Zinc 2 By similarity
	Metal binding	186	1	Zinc 2 By similarity
	Metal binding	189	1	Zinc 2 By similarity
	Metal binding	202	1	Zinc 1 By similarity
	Metal binding	205	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	78	1	Phosphoserine Ref.9 Ref.10
	Modified residue	409	1	Cysteine methyl ester Probable
	Lipidation	409	1	S-farnesyl cysteine Ref.8
Experimental info
	Sequence conflict	17	1	P → A in AAB69313. Ref.2
	Sequence conflict	42 – 46	5	NAGDK → QMQETN in AAB69313. Ref.2
	Sequence conflict	83 – 93	11	GMDDIFSHIFG → WHGLIFSLTVFC in AAB69313. Ref.2
	Sequence conflict	242 – 257	16	GVEPG…QEKEH → EWNPETLFFLLPGEKNM in AAB69313. Ref.2
	Sequence conflict	286 – 287	2	FK → LS in AAB69313. Ref.2
	Sequence conflict	328	1	D → G in AAB69313. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O60884 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 8F1BC367425CB428	FASTA	412	45,746
	10 20 30 40 50 60 MANVADTKLY DILGVPPGAS ENELKKAYRK LAKEYHPDKN PNAGDKFKEI SFAYEVLSNP 70 80 90 100 110 120 EKRELYDRYG EQGLREGSGG GGGMDDIFSH IFGGGLFGFM GNQSRSRNGR RRGEDMMHPL 130 140 150 160 170 180 KVSLEDLYNG KTTKLQLSKN VLCSACSGQG GKSGAVQKCS ACRGRGVRIM IRQLAPGMVQ 190 200 210 220 230 240 QMQSVCSDCN GEGEVINEKD RCKKCEGKKV IKEVKILEVH VDKGMKHGQR ITFTGEADQA 250 260 270 280 290 300 PGVEPGDIVL LLQEKEHEVF QRDGNDLHMT YKIGLVEALC GFQFTFKHLD GRQIVVKYPP 310 320 330 340 350 360 GKVIEPGCVR VVRGEGMPQY RNPFEKGDLY IKFDVQFPEN NWINPDKLSE LEDLLPSRPE 370 380 390 400 410 VPNIIGETEE VELQEFDSTR GSGGGQRREA YNDSSDEESS SHHGPGVQCA HQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"HIRIP4, a new human DnaJ, is a nuclear protein that interacts with the product of the DiGeorge syndrome gene candidate HIRA." Lorain S., Brendel C., Scamps C., Lecluse Y., Lipinski M. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Human CPR (cell cycle progression restoration) genes impart a Far-phenotype on yeast cells." Edwards M.C., Liegeois N., Horecka J., DePinho R.A., Sprague G.F. Jr., Tyers M., Elledge S.J. Genetics 147:1063-1076(1997) [PubMed: 9383053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta and Skin.
[6]	"Antigens recognized by autologous antibody in patients with renal-cell carcinoma." Scanlan M.J., Gordan J.D., Williamson B., Stockert E., Bander N.H., Jongeneel C.V., Gure A.O., Jaeger D., Jaeger E., Knuth A., Chen Y.-T., Old L.J. Int. J. Cancer 83:456-464(1999) [PubMed: 10508479] [Abstract] Cited for: IDENTIFICATION AS A RENAL CANCER ANTIGEN. Tissue: Renal cell carcinoma.
[7]	"Human DnaJ homologs dj2 and dj3, and bag-1 are positive cochaperones of hsc70." Terada K., Mori M. J. Biol. Chem. 275:24728-24734(2000) [PubMed: 10816573] [Abstract] Cited for: CHARACTERIZATION.
[8]	"A tagging-via-substrate technology for detection and proteomics of farnesylated proteins." Kho Y., Kim S.C., Jiang C., Barma D., Kwon S.W., Cheng J., Jaunbergs J., Weinbaum C., Tamanoi F., Falck J., Zhao Y. Proc. Natl. Acad. Sci. U.S.A. 101:12479-12484(2004) [PubMed: 15308774] [Abstract] Cited for: ISOPRENYLATION AT CYS-409.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-78, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-78, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ001309 mRNA. Translation: CAA04669.1. Y13350 mRNA. Translation: CAA73791.1. AF011793 mRNA. Translation: AAB69313.1. AK313031 mRNA. Translation: BAG35864.1. CH471092 Genomic DNA. Translation: EAW82693.1. BC013044 mRNA. Translation: AAH13044.1. BC015809 mRNA. Translation: AAH15809.1.
IPI	IPI00032406.
RefSeq	NP_005871.1. NM_005880.3.
UniGene	Hs.368078.
3D structure databases
ProteinModelPortal	O60884.
SMR	O60884. Positions 6-70, 110-379.
ModBase	Search...
Protein-protein interaction databases
IntAct	O60884. 16 interactions.
STRING	O60884.
PTM databases
PhosphoSite	O60884.
Proteomic databases
PeptideAtlas	O60884.
PRIDE	O60884.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000317089; ENSP00000314030; ENSG00000069345.
GeneID	10294.
KEGG	hsa:10294.
UCSC	uc002eeo.2. human.
Organism-specific databases
CTD	10294.
GeneCards	GC16M046931.
H-InvDB	HIX0013004.
HGNC	HGNC:14884. DNAJA2.
MIM	611322. gene.
neXtProt	NX_O60884.
PharmGKB	PA27409.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG09800.
GeneTree	ENSGT00490000043321.
HOGENOM	HBG635315.
HOVERGEN	HBG066727.
InParanoid	O60884.
OMA	NGQGGKS.
OrthoDB	EOG4T783H.
PhylomeDB	O60884.
Gene expression databases
ArrayExpress	O60884.
Bgee	O60884.
CleanEx	HS_DNAJA2.
Genevestigator	O60884.
GermOnline	ENSG00000069345. Homo sapiens.
Family and domain databases
InterPro	IPR012724. DnaJ. IPR002939. DnaJ_C. IPR001623. DnaJ_N. IPR018253. Heat_shock_DnaJ_CS. IPR008971. HSP40/DnaJ_pept-bd. IPR003095. Hsp_DnaJ. IPR001305. HSP_DnaJ_Cys-rich_dom. [Graphical view]
Gene3D	G3DSA:1.10.287.110. DnaJ_N. 1 hit. G3DSA:2.10.230.10. HSP_DnaJ_cys-rich. 1 hit.
KO	K09503.
Pfam	PF00226. DnaJ. 1 hit. PF01556. DnaJ_C. 1 hit. PF00684. DnaJ_CXXCXGXG. 1 hit. [Graphical view]
PRINTS	PR00625. JDOMAIN.
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
SUPFAM	SSF46565. DnaJ_N. 1 hit. SSF49493. HSP40_DnaJ_pep. 2 hits. SSF57938. HSP_DnaJ_cys-rich. 1 hit.
PROSITE	PS00636. DNAJ_1. 1 hit. PS50076. DNAJ_2. 1 hit. PS51188. ZF_CR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	39010.
SOURCE	Search...

Entry information

Entry name

DNJA2_HUMAN

Accession

Primary (citable) accession number: O60884
Secondary accession number(s): B2R7L7, O14711

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.