Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA/RNA-binding protein KIN17

Gene

KIN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo (By similarity). Binds via its C-terminal domain to RNA in vitro.By similarity6 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 5023C2H2-typeSequence analysisAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: ProtInc
  • double-stranded DNA binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • RNA binding Source: UniProtKB

GO - Biological processi

  • cellular response to DNA damage stimulus Source: UniProtKB
  • DNA recombination Source: UniProtKB-KW
  • DNA repair Source: UniProtKB-KW
  • DNA replication Source: UniProtKB
  • mRNA processing Source: UniProtKB
  • viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, DNA replication, Host-virus interaction, mRNA processing, Stress response

Keywords - Ligandi

DNA-binding, Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA/RNA-binding protein KIN17
Alternative name(s):
Binding to curved DNA
KIN, antigenic determinant of recA protein homolog
Gene namesi
Name:KINImported
Synonyms:BTCD, KIN17
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:6327. KIN.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: HPA
  • nuclear matrix Source: UniProtKB
  • nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi135 – 1351K → R: Almost complete loss of in vitro methylation by METTL22. 1 Publication
Mutagenesisi302 – 3021K → E: Significant reduction of RNA-binding activity. 1 Publication
Mutagenesisi391 – 3911K → E: Significant reduction of RNA-binding activity. 1 Publication

Organism-specific databases

PharmGKBiPA30113.

Polymorphism and mutation databases

BioMutaiKIN.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393DNA/RNA-binding protein KIN17PRO_0000289134Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei135 – 1351N6,N6,N6-trimethyllysine; by METTL22; in vitro1 Publication

Keywords - PTMi

Methylation

Proteomic databases

EPDiO60870.
MaxQBiO60870.
PaxDbiO60870.
PeptideAtlasiO60870.
PRIDEiO60870.

PTM databases

iPTMnetiO60870.
PhosphoSiteiO60870.

Expressioni

Tissue specificityi

Ubiquitously expressed in all tissues examined, with highest levels in skeletal muscle, heart and testis. Differentially expressed in non-tumorigenic and tumorigenic cell lines. Highly expressed in proliferating epithelial keratinocyte cells in vitro (at protein level).2 Publications

Inductioni

By UVC irradiation in quiescent primary fibroblasts. By mitomycin C in human melanoma MeWO cells.2 Publications

Gene expression databases

BgeeiENSG00000151657.
CleanExiHS_KIN.
ExpressionAtlasiO60870. baseline and differential.
GenevisibleiO60870. HS.

Organism-specific databases

HPAiCAB016339.
HPA038700.

Interactioni

Subunit structurei

Interacts with SV40 large T antigen. Associated with DNA polymerase alpha, RFC1 and cyclin A, in multiprotein DNA replication complexes. Also associates with replication origins at the G1/S phase boundary and throughout the S phase in vivo.2 Publications

Protein-protein interaction databases

BioGridi116600. 11 interactions.
IntActiO60870. 2 interactions.
MINTiMINT-248071.
STRINGi9606.ENSP00000368881.

Structurei

Secondary structure

1
393
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi61 – 633Combined sources
Helixi65 – 8319Combined sources
Beta strandi88 – 903Combined sources
Helixi91 – 988Combined sources
Helixi107 – 1093Combined sources
Helixi115 – 1228Combined sources
Turni123 – 1264Combined sources
Beta strandi127 – 1337Combined sources
Beta strandi136 – 1416Combined sources
Helixi146 – 1549Combined sources
Helixi156 – 1583Combined sources
Beta strandi285 – 2895Combined sources
Helixi296 – 2983Combined sources
Beta strandi302 – 3098Combined sources
Turni310 – 3123Combined sources
Beta strandi313 – 3186Combined sources
Turni319 – 3213Combined sources
Beta strandi324 – 3285Combined sources
Helixi329 – 3313Combined sources
Beta strandi332 – 3343Combined sources
Beta strandi342 – 3454Combined sources
Turni349 – 3524Combined sources
Beta strandi354 – 3618Combined sources
Helixi362 – 3643Combined sources
Beta strandi366 – 3716Combined sources
Turni375 – 3784Combined sources
Beta strandi380 – 3856Combined sources
Helixi386 – 3883Combined sources
Beta strandi389 – 3924Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CKKX-ray1.45A268-393[»]
2V1NNMR-A51-160[»]
ProteinModelPortaliO60870.
SMRiO60870. Positions 51-160, 274-393.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60870.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 160110Winged helix-turn-helix (wHTH)Add
BLAST
Regioni284 – 33451C-terminal subdomain A1 PublicationAdd
BLAST
Regioni340 – 39152C-terminal subdomain B1 PublicationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili147 – 18034Sequence analysisAdd
BLAST
Coiled coili250 – 27728Sequence analysisAdd
BLAST

Domaini

The C-terminal domain (268-393) is organized into 2 subdomains that bear structural similarities to SH3-like domains. Both subdomains adopt a similar 5-stranded beta-barrel-like fold and are connected to each other by a short linker of 5 residues. The 5 beta-sheets are packed at approximately right angles against each other. A highly conserved groove formed at the interface between the 2 subdomains, comprised of Lys residues 302 and 391 and other positively charged residues, may possibly be the site of RNA-binding.1 Publication

Sequence similaritiesi

Belongs to the KIN17 family.Curated
Contains 1 C2H2-type zinc finger.Sequence analysis

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 5023C2H2-typeSequence analysisAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG2837. Eukaryota.
ENOG410XRJ7. LUCA.
GeneTreeiENSGT00390000005903.
HOGENOMiHOG000178663.
HOVERGENiHBG053068.
InParanoidiO60870.
KOiK13102.
OMAiGKKERAN.
OrthoDBiEOG091G0AQX.
PhylomeDBiO60870.
TreeFamiTF314393.

Family and domain databases

InterProiIPR019447. DNA/RNA-bd_Kin17_cons_domain.
[Graphical view]
PfamiPF10357. Kin17_mid. 1 hit.
[Graphical view]
SMARTiSM01253. Kin17_mid. 1 hit.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O60870-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKSDFLTPK AIANRIKSKG LQKLRWYCQM CQKQCRDENG FKCHCMSESH
60 70 80 90 100
QRQLLLASEN PQQFMDYFSE EFRNDFLELL RRRFGTKRVH NNIVYNEYIS
110 120 130 140 150
HREHIHMNAT QWETLTDFTK WLGREGLCKV DETPKGWYIQ YIDRDPETIR
160 170 180 190 200
RQLELEKKKK QDLDDEEKTA KFIEEQVRRG LEGKEQEVPT FTELSRENDE
210 220 230 240 250
EKVTFNLSKG ACSSSGATSS KSSTLGPSAL KTIGSSASVK RKESSQSSTQ
260 270 280 290 300
SKEKKKKKSA LDEIMEIEEE KKRTARTDYW LQPEIIVKII TKKLGEKYHK
310 320 330 340 350
KKAIVKEVID KYTAVVKMID SGDKLKLDQT HLETVIPAPG KRILVLNGGY
360 370 380 390
RGNEGTLESI NEKTFSATIV IETGPLKGRR VEGIQYEDIS KLA
Length:393
Mass (Da):45,374
Last modified:January 1, 1999 - v2
Checksum:i515A89B4C8A4C007
GO
Isoform 2 (identifier: O60870-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     374-393: GPLKGRRVEGIQYEDISKLA → V

Note: No experimental confirmation available.
Show »
Length:374
Mass (Da):43,261
Checksum:i4507B25B386B68F7
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei374 – 39320GPLKG…ISKLA → V in isoform 2. 1 PublicationVSP_056074Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005273 mRNA. Translation: CAA06462.1.
AK301789 mRNA. Translation: BAG63244.1.
AL158044 Genomic DNA. Translation: CAI12959.1.
BC017309 mRNA. Translation: AAH17309.1.
CCDSiCCDS7080.1. [O60870-1]
RefSeqiNP_036443.1. NM_012311.3. [O60870-1]
XP_011517729.1. XM_011519427.1. [O60870-2]
UniGeneiHs.397918.

Genome annotation databases

EnsembliENST00000379562; ENSP00000368881; ENSG00000151657. [O60870-1]
GeneIDi22944.
KEGGihsa:22944.
UCSCiuc001ijt.4. human. [O60870-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005273 mRNA. Translation: CAA06462.1.
AK301789 mRNA. Translation: BAG63244.1.
AL158044 Genomic DNA. Translation: CAI12959.1.
BC017309 mRNA. Translation: AAH17309.1.
CCDSiCCDS7080.1. [O60870-1]
RefSeqiNP_036443.1. NM_012311.3. [O60870-1]
XP_011517729.1. XM_011519427.1. [O60870-2]
UniGeneiHs.397918.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CKKX-ray1.45A268-393[»]
2V1NNMR-A51-160[»]
ProteinModelPortaliO60870.
SMRiO60870. Positions 51-160, 274-393.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116600. 11 interactions.
IntActiO60870. 2 interactions.
MINTiMINT-248071.
STRINGi9606.ENSP00000368881.

PTM databases

iPTMnetiO60870.
PhosphoSiteiO60870.

Polymorphism and mutation databases

BioMutaiKIN.

Proteomic databases

EPDiO60870.
MaxQBiO60870.
PaxDbiO60870.
PeptideAtlasiO60870.
PRIDEiO60870.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379562; ENSP00000368881; ENSG00000151657. [O60870-1]
GeneIDi22944.
KEGGihsa:22944.
UCSCiuc001ijt.4. human. [O60870-1]

Organism-specific databases

CTDi22944.
GeneCardsiKIN.
HGNCiHGNC:6327. KIN.
HPAiCAB016339.
HPA038700.
MIMi601720. gene.
neXtProtiNX_O60870.
PharmGKBiPA30113.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2837. Eukaryota.
ENOG410XRJ7. LUCA.
GeneTreeiENSGT00390000005903.
HOGENOMiHOG000178663.
HOVERGENiHBG053068.
InParanoidiO60870.
KOiK13102.
OMAiGKKERAN.
OrthoDBiEOG091G0AQX.
PhylomeDBiO60870.
TreeFamiTF314393.

Miscellaneous databases

ChiTaRSiKIN. human.
EvolutionaryTraceiO60870.
GeneWikiiKIN_(gene).
GenomeRNAii22944.
PROiO60870.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000151657.
CleanExiHS_KIN.
ExpressionAtlasiO60870. baseline and differential.
GenevisibleiO60870. HS.

Family and domain databases

InterProiIPR019447. DNA/RNA-bd_Kin17_cons_domain.
[Graphical view]
PfamiPF10357. Kin17_mid. 1 hit.
[Graphical view]
SMARTiSM01253. Kin17_mid. 1 hit.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKIN17_HUMAN
AccessioniPrimary (citable) accession number: O60870
Secondary accession number(s): B4DX32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: January 1, 1999
Last modified: September 7, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Recognized by antibodies directed against the RecA protein.By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.