Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endothelial differentiation-related factor 1

Gene

EDF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi92 – 111H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • sequence-specific DNA binding Source: InterPro
  • transcription coactivator activity Source: UniProtKB

GO - Biological processi

  • endothelial cell differentiation Source: UniProtKB
  • multicellular organism development Source: ProtInc
  • positive regulation of DNA binding Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of lipid metabolic process Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

Calmodulin-binding, DNA-binding

Enzyme and pathway databases

SIGNORiO60869.

Names & Taxonomyi

Protein namesi
Recommended name:
Endothelial differentiation-related factor 1
Short name:
EDF-1
Alternative name(s):
Multiprotein-bridging factor 1
Short name:
MBF1
Gene namesi
Name:EDF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:3164. EDF1.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • intracellular Source: ProtInc
  • nucleolus Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi40T → D: Loss of interaction with CALM; when associated with D-58; D-91 and D-111. 1 Publication1
Mutagenesisi58T → D: Loss of interaction with CALM; when associated with D-40; D-91 and D-111. 1 Publication1
Mutagenesisi65T → D: No effect on CALM-binding. No effect; when associated with D-74. 1 Publication1
Mutagenesisi74T → D: No effect on CALM-binding. No effect; when associated with D-65. 1 Publication1
Mutagenesisi87S → A: No effect on CALM-binding. 1 Publication1
Mutagenesisi87S → D: Loss of interaction with CALM and higher affinity for TBP. Same effect; when associated with D-65 and D-74. 1 Publication1
Mutagenesisi91T → A: No effect on CALM-binding. 1 Publication1
Mutagenesisi91T → D: Partial loss of interaction with CALM. Complete loss of interaction; when associated with D-40; D-58 and D-111. 1 Publication1
Mutagenesisi111S → D: Loss of interaction with CALM; when associated with D-40; D-58 and D-91. 1 Publication1

Organism-specific databases

DisGeNETi8721.
OpenTargetsiENSG00000107223.
PharmGKBiPA27604.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001497952 – 148Endothelial differentiation-related factor 1Add BLAST147

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei4PhosphoserineCombined sources1
Modified residuei25N6-methyllysineCombined sources1

Post-translational modificationi

Phosphorylated (by PKA and PKC).2 Publications

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiO60869.
MaxQBiO60869.
PaxDbiO60869.
PeptideAtlasiO60869.
PRIDEiO60869.
TopDownProteomicsiO60869-1. [O60869-1]

PTM databases

iPTMnetiO60869.
PhosphoSitePlusiO60869.

Expressioni

Tissue specificityi

Expressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues.3 Publications

Developmental stagei

Expressed in fetal tissues. More abundant in kidney.1 Publication

Inductioni

Down-regulated by HIV-1 Tat or phorbol ester (TPA) treatment in endothelial cells (at mRNA and protein levels).1 Publication

Gene expression databases

BgeeiENSG00000107223.
CleanExiHS_EDF1.
GenevisibleiO60869. HS.

Organism-specific databases

HPAiHPA035642.

Interactioni

Subunit structurei

Interacts with TBP and the transcription factor IID (TFIID) complex, NR5A2, NR1H3 and PPARG. Interaction with TBP is regulated by phosphorylation. Binds NR5A1, ATF1, FOS and JUN via their conserved basic region. Binding to calmodulin is regulated by calcium and phosphorylation of the IQ motif.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MEOX2A4D1273EBI-781301,EBI-10172134
NR1H3Q131334EBI-781301,EBI-781356
NR5A1Q047524EBI-781310,EBI-850837From a different organism.
NR5A2O004822EBI-781301,EBI-781320
NSP034952EBI-781301,EBI-2548993From a different organism.
PPARGP372314EBI-781301,EBI-781384
TBPP202262EBI-781310,EBI-355371

Protein-protein interaction databases

BioGridi114260. 24 interactors.
IntActiO60869. 16 interactors.
MINTiMINT-3000548.
STRINGi9606.ENSP00000224073.

Structurei

Secondary structure

1148
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi76 – 86Combined sources11
Turni87 – 89Combined sources3
Helixi92 – 99Combined sources8
Helixi103 – 111Combined sources9
Helixi118 – 128Combined sources11
Turni135 – 138Combined sources4
Beta strandi140 – 143Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X57NMR-A71-148[»]
ProteinModelPortaliO60869.
SMRiO60869.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60869.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 135HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST55

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 113Interaction with NR5A2, PPARG and NR1H31 PublicationAdd BLAST77
Regioni69 – 108Interaction with TBP and NR5A11 PublicationAdd BLAST40

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi81 – 88IQ motif8

Domaini

The IQ motif, which is involved in calmodulin binding, overlaps with the binding domain for nuclear receptors and transcription factors. Its phosphorylation probably allows a switch between the two activities of the protein (By similarity).By similarity

Sequence similaritiesi

Contains 1 HTH cro/C1-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3398. Eukaryota.
COG1813. LUCA.
GeneTreeiENSGT00390000008519.
HOGENOMiHOG000195726.
HOVERGENiHBG051440.
InParanoidiO60869.
KOiK03627.
OMAiYECGRAI.
OrthoDBiEOG091G0RRN.
PhylomeDBiO60869.
TreeFamiTF300064.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR013729. MBF1_N.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
PF08523. MBF1. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O60869-1) [UniParc]FASTAAdd to basket
Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK
60 70 80 90 100
QHSITKNTAK LDRETEELHH DRVTLEVGKV IQQGRQSKGL TQKDLATKIN
110 120 130 140
EKPQVIADYE SGRAIPNNQV LGKIERAIGL KLRGKDIGKP IEKGPRAK
Length:148
Mass (Da):16,369
Last modified:August 1, 1998 - v1
Checksum:iADC2C5384BF85C11
GO
Isoform 2 (identifier: O60869-2) [UniParc]FASTAAdd to basket
Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     130-148: LKLRGKDIGKPIEKGPRAK → ECPSTLRRVR

Show »
Length:139
Mass (Da):15,481
Checksum:i9F30DE6890D7E801
GO
Isoform 3 (identifier: O60869-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     129-148: GLKLRGKDIGKPIEKGPRAK → DVGTRSARVLRAQ

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:141
Mass (Da):15,636
Checksum:iE31C8E26A4679A23
GO

Sequence cautioni

The sequence CAI12698 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054701129 – 148GLKLR…GPRAK → DVGTRSARVLRAQ in isoform 3. CuratedAdd BLAST20
Alternative sequenceiVSP_013336130 – 148LKLRG…GPRAK → ECPSTLRRVR in isoform 2. 1 PublicationAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005259 mRNA. Translation: CAA06446.1.
AB002282 mRNA. Translation: BAA88073.1.
AB002283 mRNA. Translation: BAA88074.1.
CR541914 mRNA. Translation: CAG46712.1.
BT009863 mRNA. Translation: AAP88865.1.
AL355987 Genomic DNA. Translation: CAI12697.1.
AL355987 Genomic DNA. Translation: CAI12698.1. Sequence problems.
AL355987 Genomic DNA. Translation: CAI12699.1.
BC015500 mRNA. Translation: AAH15500.1.
CCDSiCCDS65193.1. [O60869-3]
CCDS7011.1. [O60869-1]
CCDS7012.1. [O60869-2]
RefSeqiNP_001268226.1. NM_001281297.1. [O60869-3]
NP_001268227.1. NM_001281298.1.
NP_001268228.1. NM_001281299.1.
NP_003783.1. NM_003792.3. [O60869-1]
NP_694880.1. NM_153200.2. [O60869-2]
UniGeneiHs.174050.

Genome annotation databases

EnsembliENST00000224073; ENSP00000224073; ENSG00000107223. [O60869-1]
ENST00000371648; ENSP00000360711; ENSG00000107223. [O60869-2]
ENST00000371649; ENSP00000360712; ENSG00000107223. [O60869-3]
GeneIDi8721.
KEGGihsa:8721.
UCSCiuc004cjt.3. human. [O60869-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005259 mRNA. Translation: CAA06446.1.
AB002282 mRNA. Translation: BAA88073.1.
AB002283 mRNA. Translation: BAA88074.1.
CR541914 mRNA. Translation: CAG46712.1.
BT009863 mRNA. Translation: AAP88865.1.
AL355987 Genomic DNA. Translation: CAI12697.1.
AL355987 Genomic DNA. Translation: CAI12698.1. Sequence problems.
AL355987 Genomic DNA. Translation: CAI12699.1.
BC015500 mRNA. Translation: AAH15500.1.
CCDSiCCDS65193.1. [O60869-3]
CCDS7011.1. [O60869-1]
CCDS7012.1. [O60869-2]
RefSeqiNP_001268226.1. NM_001281297.1. [O60869-3]
NP_001268227.1. NM_001281298.1.
NP_001268228.1. NM_001281299.1.
NP_003783.1. NM_003792.3. [O60869-1]
NP_694880.1. NM_153200.2. [O60869-2]
UniGeneiHs.174050.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X57NMR-A71-148[»]
ProteinModelPortaliO60869.
SMRiO60869.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114260. 24 interactors.
IntActiO60869. 16 interactors.
MINTiMINT-3000548.
STRINGi9606.ENSP00000224073.

PTM databases

iPTMnetiO60869.
PhosphoSitePlusiO60869.

Proteomic databases

EPDiO60869.
MaxQBiO60869.
PaxDbiO60869.
PeptideAtlasiO60869.
PRIDEiO60869.
TopDownProteomicsiO60869-1. [O60869-1]

Protocols and materials databases

DNASUi8721.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000224073; ENSP00000224073; ENSG00000107223. [O60869-1]
ENST00000371648; ENSP00000360711; ENSG00000107223. [O60869-2]
ENST00000371649; ENSP00000360712; ENSG00000107223. [O60869-3]
GeneIDi8721.
KEGGihsa:8721.
UCSCiuc004cjt.3. human. [O60869-1]

Organism-specific databases

CTDi8721.
DisGeNETi8721.
GeneCardsiEDF1.
HGNCiHGNC:3164. EDF1.
HPAiHPA035642.
MIMi605107. gene.
neXtProtiNX_O60869.
OpenTargetsiENSG00000107223.
PharmGKBiPA27604.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3398. Eukaryota.
COG1813. LUCA.
GeneTreeiENSGT00390000008519.
HOGENOMiHOG000195726.
HOVERGENiHBG051440.
InParanoidiO60869.
KOiK03627.
OMAiYECGRAI.
OrthoDBiEOG091G0RRN.
PhylomeDBiO60869.
TreeFamiTF300064.

Enzyme and pathway databases

SIGNORiO60869.

Miscellaneous databases

ChiTaRSiEDF1. human.
EvolutionaryTraceiO60869.
GeneWikiiEDF1.
GenomeRNAii8721.
PROiO60869.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000107223.
CleanExiHS_EDF1.
GenevisibleiO60869. HS.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR013729. MBF1_N.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
PF08523. MBF1. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEDF1_HUMAN
AccessioniPrimary (citable) accession number: O60869
Secondary accession number(s): Q5T5T2, Q9UIM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.