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Protein

PRA1 family protein 2

Gene

PRAF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis.2 Publications

GO - Biological processi

  1. L-glutamate transport Source: UniProtKB
  2. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
PRA1 family protein 2
Gene namesi
Name:PRAF2
ORF Names:JM4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:28911. PRAF2.

Subcellular locationi

Endosome membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4141CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei42 – 6221HelicalSequence AnalysisAdd
BLAST
Topological domaini63 – 642ExtracellularSequence Analysis
Transmembranei65 – 8521HelicalSequence AnalysisAdd
BLAST
Topological domaini86 – 9611CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei97 – 11923HelicalSequence AnalysisAdd
BLAST
Topological domaini120 – 1223ExtracellularSequence Analysis
Transmembranei123 – 14018HelicalSequence AnalysisAdd
BLAST
Topological domaini141 – 17838CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endosome membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134955002.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 178178PRA1 family protein 2PRO_0000220881Add
BLAST

Proteomic databases

MaxQBiO60831.
PaxDbiO60831.
PeptideAtlasiO60831.
PRIDEiO60831.

Expressioni

Tissue specificityi

Strong expression in the brain, small intestine, lung, spleen, and pancreas as well as in tumor tissues of the breast, colon, lung and ovary, with a weaker expression in normal tissues of the same patient. High expression in neuroblastic tumors. Strongly expressed in Purkinje cells and more moderately in cells of the molecular and the granular layers in the cerebellum. Detected in neuronal cells, but not in non-neuronal cells in the cerebral cortex, hippocampus, and lateral ventricles.3 Publications

Gene expression databases

BgeeiO60831.
CleanExiHS_PRAF2.
ExpressionAtlasiO60831. baseline and differential.
GenevestigatoriO60831.

Organism-specific databases

HPAiCAB011655.
HPA002859.

Interactioni

Subunit structurei

Interacts with CCR5 and GDE1.2 Publications

Protein-protein interaction databases

BioGridi116396. 10 interactions.
IntActiO60831. 2 interactions.
MINTiMINT-1339907.
STRINGi9606.ENSP00000365570.

Structurei

3D structure databases

ProteinModelPortaliO60831.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PRA1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG288107.
GeneTreeiENSGT00390000008631.
HOGENOMiHOG000231363.
HOVERGENiHBG053661.
InParanoidiO60831.
OMAiLDRWNNR.
OrthoDBiEOG7VDXQH.
PhylomeDBiO60831.
TreeFamiTF105479.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60831-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEVRLPPLR ALDDFVLGSA RLAAPDPCDP QRWCHRVINN LLYYQTNYLL
60 70 80 90 100
CFGIGLALAG YVRPLHTLLS ALVVAVALGV LVWAAETRAA VRRCRRSHPA
110 120 130 140 150
ACLAAVLAVG LLVLWVAGGA CTFLFSIAGP VLLILVHASL RLRNLKNKIE
160 170
NKIESIGLKR TPMGLLLEAL GQEQEAGS
Length:178
Mass (Da):19,258
Last modified:August 1, 1998 - v1
Checksum:iE62695D7DC40550A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti56 – 561L → F.
Corresponds to variant rs34565429 [ dbSNP | Ensembl ].
VAR_050628

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005896 mRNA. Translation: CAA06753.1.
CR457023 mRNA. Translation: CAG33304.1.
AK315374 mRNA. Translation: BAG37767.1.
CH471224 Genomic DNA. Translation: EAW50706.1.
BC021213 mRNA. Translation: AAH21213.1.
CCDSiCCDS14317.1.
RefSeqiNP_009144.1. NM_007213.2.
UniGeneiHs.29595.

Genome annotation databases

EnsembliENST00000553851; ENSP00000451962; ENSG00000243279.
GeneIDi11230.
KEGGihsa:11230.
UCSCiuc004dmi.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005896 mRNA. Translation: CAA06753.1.
CR457023 mRNA. Translation: CAG33304.1.
AK315374 mRNA. Translation: BAG37767.1.
CH471224 Genomic DNA. Translation: EAW50706.1.
BC021213 mRNA. Translation: AAH21213.1.
CCDSiCCDS14317.1.
RefSeqiNP_009144.1. NM_007213.2.
UniGeneiHs.29595.

3D structure databases

ProteinModelPortaliO60831.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116396. 10 interactions.
IntActiO60831. 2 interactions.
MINTiMINT-1339907.
STRINGi9606.ENSP00000365570.

Proteomic databases

MaxQBiO60831.
PaxDbiO60831.
PeptideAtlasiO60831.
PRIDEiO60831.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000553851; ENSP00000451962; ENSG00000243279.
GeneIDi11230.
KEGGihsa:11230.
UCSCiuc004dmi.3. human.

Organism-specific databases

CTDi11230.
GeneCardsiGC0XM048928.
HGNCiHGNC:28911. PRAF2.
HPAiCAB011655.
HPA002859.
MIMi300840. gene.
neXtProtiNX_O60831.
PharmGKBiPA134955002.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG288107.
GeneTreeiENSGT00390000008631.
HOGENOMiHOG000231363.
HOVERGENiHBG053661.
InParanoidiO60831.
OMAiLDRWNNR.
OrthoDBiEOG7VDXQH.
PhylomeDBiO60831.
TreeFamiTF105479.

Miscellaneous databases

ChiTaRSiPRAF2. human.
GenomeRNAii11230.
NextBioi42738.
PROiO60831.
SOURCEiSearch...

Gene expression databases

BgeeiO60831.
CleanExiHS_PRAF2.
ExpressionAtlasiO60831. baseline and differential.
GenevestigatoriO60831.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Transcription map in Xp11.23."
    Strom T.M., Nyakatura G., Hellebrand H., Drescher B., Rosenthal A., Meindl A.
    Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Amygdala.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: B-cell.
  6. "JM4 is a four-transmembrane protein binding to the CCR5 receptor."
    Schweneker M., Bachmann A.S., Moelling K.
    FEBS Lett. 579:1751-1758(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CCR5.
  7. "Genomic organization, expression profile, and characterization of the new protein PRA1 domain family, member 2 (PRAF2)."
    Fo C.S., Coleman C.S., Wallick C.J., Vine A.L., Bachmann A.S.
    Gene 371:154-165(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOMIC ORGANIZATION, TISSUE SPECIFICITY.
  8. "Genomic organization, characterization, and molecular 3D model of GDE1, a novel mammalian glycerophosphoinositol phosphodiesterase."
    Bachmann A.S., Duennebier F.F., Mocz G.
    Gene 371:144-153(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GDE1.
  9. "Expression of prenylated Rab acceptor 1 domain family, member 2 (PRAF2) in neuroblastoma: correlation with clinical features, cellular localization, and cerulenin-mediated apoptosis regulation."
    Geerts D., Wallick C.J., Koomoa D.-L., Koster J., Versteeg R., Go R.C.V., Bachmann A.S.
    Clin. Cancer Res. 13:6312-6319(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  10. "Expression profile of PRAF2 in the human brain and enrichment in synaptic vesicles."
    Koomoa D.-L., Go R.C.V., Wester K., Bachmann A.S.
    Neurosci. Lett. 436:171-176(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPRAF2_HUMAN
AccessioniPrimary (citable) accession number: O60831
Secondary accession number(s): B2RD20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: August 1, 1998
Last modified: January 7, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.