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O60831 (PRAF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PRA1 family protein 2
Gene names
Name:PRAF2
ORF Names:JM4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length178 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis. Ref.9 Ref.10

Subunit structure

Interacts with CCR5 and GDE1. Ref.6 Ref.8

Subcellular location

Endosome membrane; Multi-pass membrane protein Probable Ref.9.

Tissue specificity

Strong expression in the brain, small intestine, lung, spleen, and pancreas as well as in tumor tissues of the breast, colon, lung and ovary, with a weaker expression in normal tissues of the same patient. High expression in neuroblastic tumors. Strongly expressed in Purkinje cells and more moderately in cells of the molecular and the granular layers in the cerebellum. Detected in neuronal cells, but not in non-neuronal cells in the cerebral cortex, hippocampus, and lateral ventricles. Ref.7 Ref.9 Ref.10

Sequence similarities

Belongs to the PRA1 family.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentEndosome
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-glutamate transport

Inferred from sequence or structural similarity. Source: UniProtKB

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentendosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 178178PRA1 family protein 2
PRO_0000220881

Regions

Topological domain1 – 4141Cytoplasmic Potential
Transmembrane42 – 6221Helical; Potential
Topological domain63 – 642Extracellular Potential
Transmembrane65 – 8521Helical; Potential
Topological domain86 – 9611Cytoplasmic Potential
Transmembrane97 – 11923Helical; Potential
Topological domain120 – 1223Extracellular Potential
Transmembrane123 – 14018Helical; Potential
Topological domain141 – 17838Cytoplasmic Potential

Natural variations

Natural variant561L → F.
Corresponds to variant rs34565429 [ dbSNP | Ensembl ].
VAR_050628

Sequences

Sequence LengthMass (Da)Tools
O60831 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: E62695D7DC40550A

FASTA17819,258
        10         20         30         40         50         60 
MSEVRLPPLR ALDDFVLGSA RLAAPDPCDP QRWCHRVINN LLYYQTNYLL CFGIGLALAG 

        70         80         90        100        110        120 
YVRPLHTLLS ALVVAVALGV LVWAAETRAA VRRCRRSHPA ACLAAVLAVG LLVLWVAGGA 

       130        140        150        160        170 
CTFLFSIAGP VLLILVHASL RLRNLKNKIE NKIESIGLKR TPMGLLLEAL GQEQEAGS 

« Hide

References

« Hide 'large scale' references
[1]"Transcription map in Xp11.23."
Strom T.M., Nyakatura G., Hellebrand H., Drescher B., Rosenthal A., Meindl A.
Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Amygdala.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: B-cell.
[6]"JM4 is a four-transmembrane protein binding to the CCR5 receptor."
Schweneker M., Bachmann A.S., Moelling K.
FEBS Lett. 579:1751-1758(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CCR5.
[7]"Genomic organization, expression profile, and characterization of the new protein PRA1 domain family, member 2 (PRAF2)."
Fo C.S., Coleman C.S., Wallick C.J., Vine A.L., Bachmann A.S.
Gene 371:154-165(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOMIC ORGANIZATION, TISSUE SPECIFICITY.
[8]"Genomic organization, characterization, and molecular 3D model of GDE1, a novel mammalian glycerophosphoinositol phosphodiesterase."
Bachmann A.S., Duennebier F.F., Mocz G.
Gene 371:144-153(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GDE1.
[9]"Expression of prenylated Rab acceptor 1 domain family, member 2 (PRAF2) in neuroblastoma: correlation with clinical features, cellular localization, and cerulenin-mediated apoptosis regulation."
Geerts D., Wallick C.J., Koomoa D.-L., Koster J., Versteeg R., Go R.C.V., Bachmann A.S.
Clin. Cancer Res. 13:6312-6319(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[10]"Expression profile of PRAF2 in the human brain and enrichment in synaptic vesicles."
Koomoa D.-L., Go R.C.V., Wester K., Bachmann A.S.
Neurosci. Lett. 436:171-176(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ005896 mRNA. Translation: CAA06753.1.
CR457023 mRNA. Translation: CAG33304.1.
AK315374 mRNA. Translation: BAG37767.1.
CH471224 Genomic DNA. Translation: EAW50706.1.
BC021213 mRNA. Translation: AAH21213.1.
RefSeqNP_009144.1. NM_007213.2.
UniGeneHs.29595.

3D structure databases

ProteinModelPortalO60831.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116396. 1 interaction.
IntActO60831. 2 interactions.
MINTMINT-1339907.
STRING9606.ENSP00000365570.

Proteomic databases

PaxDbO60831.
PeptideAtlasO60831.
PRIDEO60831.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000376390; ENSP00000365570; ENSG00000243279.
GeneID11230.
KEGGhsa:11230.
UCSCuc004dmi.3. human.

Organism-specific databases

CTD11230.
GeneCardsGC0XM048928.
HGNCHGNC:28911. PRAF2.
HPACAB011655.
HPA002859.
MIM300840. gene.
neXtProtNX_O60831.
PharmGKBPA134955002.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG288107.
HOGENOMHOG000231363.
HOVERGENHBG053661.
InParanoidO60831.
OMARRFRRNH.
OrthoDBEOG7VDXQH.
PhylomeDBO60831.
TreeFamTF105479.

Gene expression databases

ArrayExpressO60831.
BgeeO60831.
CleanExHS_PRAF2.
GenevestigatorO60831.

Family and domain databases

InterProIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamPF03208. PRA1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPRAF2. human.
GenomeRNAi11230.
NextBio42738.
PROO60831.
SOURCESearch...

Entry information

Entry namePRAF2_HUMAN
AccessionPrimary (citable) accession number: O60831
Secondary accession number(s): B2RD20
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: August 1, 1998
Last modified: February 19, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM