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O60831

- PRAF2_HUMAN

UniProt

O60831 - PRAF2_HUMAN

Protein

PRA1 family protein 2

Gene

PRAF2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis.2 Publications

    GO - Biological processi

    1. L-glutamate transport Source: UniProtKB
    2. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    PRA1 family protein 2
    Gene namesi
    Name:PRAF2
    ORF Names:JM4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome X

    Organism-specific databases

    HGNCiHGNC:28911. PRAF2.

    Subcellular locationi

    Endosome membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endosome membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endosome, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134955002.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 178178PRA1 family protein 2PRO_0000220881Add
    BLAST

    Proteomic databases

    MaxQBiO60831.
    PaxDbiO60831.
    PeptideAtlasiO60831.
    PRIDEiO60831.

    Expressioni

    Tissue specificityi

    Strong expression in the brain, small intestine, lung, spleen, and pancreas as well as in tumor tissues of the breast, colon, lung and ovary, with a weaker expression in normal tissues of the same patient. High expression in neuroblastic tumors. Strongly expressed in Purkinje cells and more moderately in cells of the molecular and the granular layers in the cerebellum. Detected in neuronal cells, but not in non-neuronal cells in the cerebral cortex, hippocampus, and lateral ventricles.3 Publications

    Gene expression databases

    ArrayExpressiO60831.
    BgeeiO60831.
    CleanExiHS_PRAF2.
    GenevestigatoriO60831.

    Organism-specific databases

    HPAiCAB011655.
    HPA002859.

    Interactioni

    Subunit structurei

    Interacts with CCR5 and GDE1.2 Publications

    Protein-protein interaction databases

    BioGridi116396. 1 interaction.
    IntActiO60831. 2 interactions.
    MINTiMINT-1339907.
    STRINGi9606.ENSP00000365570.

    Structurei

    3D structure databases

    ProteinModelPortaliO60831.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4141CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini63 – 642ExtracellularSequence Analysis
    Topological domaini86 – 9611CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini120 – 1223ExtracellularSequence Analysis
    Topological domaini141 – 17838CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei42 – 6221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei65 – 8521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei97 – 11923HelicalSequence AnalysisAdd
    BLAST
    Transmembranei123 – 14018HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PRA1 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG288107.
    HOGENOMiHOG000231363.
    HOVERGENiHBG053661.
    InParanoidiO60831.
    OMAiERWNNRI.
    OrthoDBiEOG7VDXQH.
    PhylomeDBiO60831.
    TreeFamiTF105479.

    Family and domain databases

    InterProiIPR004895. Prenylated_rab_accept_PRA1.
    [Graphical view]
    PfamiPF03208. PRA1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O60831-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEVRLPPLR ALDDFVLGSA RLAAPDPCDP QRWCHRVINN LLYYQTNYLL    50
    CFGIGLALAG YVRPLHTLLS ALVVAVALGV LVWAAETRAA VRRCRRSHPA 100
    ACLAAVLAVG LLVLWVAGGA CTFLFSIAGP VLLILVHASL RLRNLKNKIE 150
    NKIESIGLKR TPMGLLLEAL GQEQEAGS 178
    Length:178
    Mass (Da):19,258
    Last modified:August 1, 1998 - v1
    Checksum:iE62695D7DC40550A
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti56 – 561L → F.
    Corresponds to variant rs34565429 [ dbSNP | Ensembl ].
    VAR_050628

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005896 mRNA. Translation: CAA06753.1.
    CR457023 mRNA. Translation: CAG33304.1.
    AK315374 mRNA. Translation: BAG37767.1.
    CH471224 Genomic DNA. Translation: EAW50706.1.
    BC021213 mRNA. Translation: AAH21213.1.
    CCDSiCCDS14317.1.
    RefSeqiNP_009144.1. NM_007213.2.
    UniGeneiHs.29595.

    Genome annotation databases

    EnsembliENST00000376390; ENSP00000365570; ENSG00000243279.
    GeneIDi11230.
    KEGGihsa:11230.
    UCSCiuc004dmi.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005896 mRNA. Translation: CAA06753.1 .
    CR457023 mRNA. Translation: CAG33304.1 .
    AK315374 mRNA. Translation: BAG37767.1 .
    CH471224 Genomic DNA. Translation: EAW50706.1 .
    BC021213 mRNA. Translation: AAH21213.1 .
    CCDSi CCDS14317.1.
    RefSeqi NP_009144.1. NM_007213.2.
    UniGenei Hs.29595.

    3D structure databases

    ProteinModelPortali O60831.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116396. 1 interaction.
    IntActi O60831. 2 interactions.
    MINTi MINT-1339907.
    STRINGi 9606.ENSP00000365570.

    Proteomic databases

    MaxQBi O60831.
    PaxDbi O60831.
    PeptideAtlasi O60831.
    PRIDEi O60831.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000376390 ; ENSP00000365570 ; ENSG00000243279 .
    GeneIDi 11230.
    KEGGi hsa:11230.
    UCSCi uc004dmi.3. human.

    Organism-specific databases

    CTDi 11230.
    GeneCardsi GC0XM048928.
    HGNCi HGNC:28911. PRAF2.
    HPAi CAB011655.
    HPA002859.
    MIMi 300840. gene.
    neXtProti NX_O60831.
    PharmGKBi PA134955002.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG288107.
    HOGENOMi HOG000231363.
    HOVERGENi HBG053661.
    InParanoidi O60831.
    OMAi ERWNNRI.
    OrthoDBi EOG7VDXQH.
    PhylomeDBi O60831.
    TreeFami TF105479.

    Miscellaneous databases

    ChiTaRSi PRAF2. human.
    GenomeRNAii 11230.
    NextBioi 42738.
    PROi O60831.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O60831.
    Bgeei O60831.
    CleanExi HS_PRAF2.
    Genevestigatori O60831.

    Family and domain databases

    InterProi IPR004895. Prenylated_rab_accept_PRA1.
    [Graphical view ]
    Pfami PF03208. PRA1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Transcription map in Xp11.23."
      Strom T.M., Nyakatura G., Hellebrand H., Drescher B., Rosenthal A., Meindl A.
      Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Amygdala.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: B-cell.
    6. "JM4 is a four-transmembrane protein binding to the CCR5 receptor."
      Schweneker M., Bachmann A.S., Moelling K.
      FEBS Lett. 579:1751-1758(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CCR5.
    7. "Genomic organization, expression profile, and characterization of the new protein PRA1 domain family, member 2 (PRAF2)."
      Fo C.S., Coleman C.S., Wallick C.J., Vine A.L., Bachmann A.S.
      Gene 371:154-165(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOMIC ORGANIZATION, TISSUE SPECIFICITY.
    8. "Genomic organization, characterization, and molecular 3D model of GDE1, a novel mammalian glycerophosphoinositol phosphodiesterase."
      Bachmann A.S., Duennebier F.F., Mocz G.
      Gene 371:144-153(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GDE1.
    9. "Expression of prenylated Rab acceptor 1 domain family, member 2 (PRAF2) in neuroblastoma: correlation with clinical features, cellular localization, and cerulenin-mediated apoptosis regulation."
      Geerts D., Wallick C.J., Koomoa D.-L., Koster J., Versteeg R., Go R.C.V., Bachmann A.S.
      Clin. Cancer Res. 13:6312-6319(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    10. "Expression profile of PRAF2 in the human brain and enrichment in synaptic vesicles."
      Koomoa D.-L., Go R.C.V., Wester K., Bachmann A.S.
      Neurosci. Lett. 436:171-176(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPRAF2_HUMAN
    AccessioniPrimary (citable) accession number: O60831
    Secondary accession number(s): B2RD20
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome X
      Human chromosome X: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3