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Protein

General vesicular transport factor p115

Gene

USO1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.By similarity

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • poly(A) RNA binding Source: UniProtKB
  • protein transporter activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000138768-MONOMER.
ReactomeiR-HSA-162658. Golgi Cisternae Pericentriolar Stack Reorganization.
R-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-6807878. COPI-mediated anterograde transport.
SIGNORiO60763.

Names & Taxonomyi

Protein namesi
Recommended name:
General vesicular transport factor p115
Alternative name(s):
Protein USO1 homolog
Transcytosis-associated protein
Short name:
TAP
Vesicle-docking protein
Gene namesi
Name:USO1
Synonyms:VDP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:30904. USO1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi942S → A: Loss of phosphorylation. Promotes association with Golgi membranes. 1 Publication1
Mutagenesisi942S → D: Decreased association with Golgi membranes. 1 Publication1

Organism-specific databases

DisGeNETi8615.
OpenTargetsiENSG00000138768.
PharmGKBiPA162408713.

Polymorphism and mutation databases

BioMutaiUSO1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000657741 – 962General vesicular transport factor p115Add BLAST962

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50PhosphoserineCombined sources1
Modified residuei202N6-acetyllysineCombined sources1
Modified residuei942PhosphoserineCombined sources1 Publication1
Modified residuei952PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO60763.
MaxQBiO60763.
PeptideAtlasiO60763.
PRIDEiO60763.

PTM databases

iPTMnetiO60763.
PhosphoSitePlusiO60763.
SwissPalmiO60763.

Expressioni

Gene expression databases

BgeeiENSG00000138768.
CleanExiHS_USO1.
ExpressionAtlasiO60763. baseline and differential.
GenevisibleiO60763. HS.

Organism-specific databases

HPAiCAB010108.
HPA038282.
HPA038283.

Interactioni

Subunit structurei

Homodimer. Dimerizes by parallel association of the tails, resulting in an elongated structure with two globular head domains side by side, and a long rod-like tail structure (Probable). Interacts with MIF.Curated2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AKAP9Q99996-23EBI-356164,EBI-9641546
DUSP12Q9UNI63EBI-356164,EBI-715161
PHYKPLQ8IUZ53EBI-356164,EBI-751947
XPO7Q9UIA93EBI-356164,EBI-286668

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL

Protein-protein interaction databases

BioGridi114173. 67 interactors.
IntActiO60763. 25 interactors.
MINTiMINT-1136055.

Structurei

Secondary structure

1962
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni57 – 62Combined sources6
Helixi63 – 71Combined sources9
Helixi76 – 91Combined sources16
Helixi112 – 119Combined sources8
Helixi122 – 130Combined sources9
Helixi136 – 152Combined sources17
Helixi154 – 163Combined sources10
Helixi167 – 172Combined sources6
Helixi173 – 176Combined sources4
Helixi180 – 194Combined sources15
Helixi198 – 206Combined sources9
Helixi209 – 219Combined sources11
Helixi222 – 224Combined sources3
Helixi227 – 240Combined sources14
Helixi244 – 252Combined sources9
Helixi256 – 259Combined sources4
Helixi261 – 263Combined sources3
Helixi274 – 290Combined sources17
Helixi297 – 309Combined sources13
Helixi312 – 321Combined sources10
Helixi327 – 341Combined sources15
Helixi345 – 352Combined sources8
Helixi364 – 372Combined sources9
Helixi379 – 393Combined sources15
Helixi397 – 405Combined sources9
Beta strandi414 – 416Combined sources3
Helixi421 – 429Combined sources9
Helixi434 – 448Combined sources15
Helixi452 – 458Combined sources7
Beta strandi466 – 469Combined sources4
Helixi474 – 481Combined sources8
Turni482 – 485Combined sources4
Helixi488 – 502Combined sources15
Helixi506 – 513Combined sources8
Helixi518 – 527Combined sources10
Turni531 – 534Combined sources4
Helixi535 – 550Combined sources16
Beta strandi557 – 559Combined sources3
Helixi561 – 571Combined sources11
Helixi574 – 582Combined sources9
Turni583 – 586Combined sources4
Helixi590 – 593Combined sources4
Helixi604 – 606Combined sources3
Helixi611 – 627Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2W3CX-ray2.22A53-629[»]
ProteinModelPortaliO60763.
SMRiO60763.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60763.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati20 – 60ARM 1Add BLAST41
Repeati61 – 121ARM 2Add BLAST61
Repeati123 – 163ARM 3Add BLAST41
Repeati166 – 207ARM 4Add BLAST42
Repeati208 – 253ARM 5Add BLAST46
Repeati255 – 310ARM 6Add BLAST56
Repeati311 – 354ARM 7Add BLAST44
Repeati363 – 408ARM 8Add BLAST46
Repeati420 – 459ARM 9Add BLAST40
Repeati473 – 513ARM 10Add BLAST41
Repeati518 – 571ARM 11Add BLAST54
Repeati573 – 630ARM 12Add BLAST58

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 637Globular headAdd BLAST637

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili638 – 930Sequence analysisAdd BLAST293

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi935 – 962Asp/Glu-rich (acidic)Add BLAST28

Domaini

Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.1 Publication

Sequence similaritiesi

Belongs to the VDP/USO1/EDE1 family.Curated
Contains 12 ARM repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

GeneTreeiENSGT00390000017018.
HOGENOMiHOG000016409.
HOVERGENiHBG018067.
InParanoidiO60763.
OMAiGYQVNQD.
OrthoDBiEOG091G046H.
PhylomeDBiO60763.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR006955. Uso1_p115_C.
IPR024095. Vesicle_P115-like.
IPR006953. Vesicle_Uso1_P115_head.
[Graphical view]
PANTHERiPTHR10013. PTHR10013. 1 hit.
PfamiPF04871. Uso1_p115_C. 1 hit.
PF04869. Uso1_p115_head. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O60763-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNFLRGVMGG QSAGPQHTEA ETIQKLCDRV ASSTLLDDRR NAVRALKSLS
60 70 80 90 100
KKYRLEVGIQ AMEHLIHVLQ TDRSDSEIIG YALDTLYNII SNEEEEEVEE
110 120 130 140 150
NSTRQSEDLG SQFTEIFIKQ QENVTLLLSL LEEFDFHVRW PGVKLLTSLL
160 170 180 190 200
KQLGPQVQQI ILVSPMGVSR LMDLLADSRE VIRNDGVLLL QALTRSNGAI
210 220 230 240 250
QKIVAFENAF ERLLDIISEE GNSDGGIVVE DCLILLQNLL KNNNSNQNFF
260 270 280 290 300
KEGSYIQRMK PWFEVGDENS GWSAQKVTNL HLMLQLVRVL VSPTNPPGAT
310 320 330 340 350
SSCQKAMFQC GLLQQLCTIL MATGVPADIL TETINTVSEV IRGCQVNQDY
360 370 380 390 400
FASVNAPSNP PRPAIVVLLM SMVNERQPFV LRCAVLYCFQ CFLYKNQKGQ
410 420 430 440 450
GEIVSTLLPS TIDATGNSVS AGQLLCGGLF STDSLSNWCA AVALAHALQE
460 470 480 490 500
NATQKEQLLR VQLATSIGNP PVSLLQQCTN ILSQGSKIQT RVGLLMLLCT
510 520 530 540 550
WLSNCPIAVT HFLHNSANVP FLTGQIAENL GEEEQLVQGL CALLLGISIY
560 570 580 590 600
FNDNSLESYM KEKLKQLIEK RIGKENFIEK LGFISKHELY SRASQKPQPN
610 620 630 640 650
FPSPEYMIFD HEFTKLVKEL EGVITKAIYK SSEEDKKEEE VKKTLEQHDN
660 670 680 690 700
IVTHYKNMIR EQDLQLEELR QQVSTLKCQN EQLQTAVTQQ VSQIQQHKDQ
710 720 730 740 750
YNLLKIQLGK DNQHQGSYSE GAQMNGIQPE EIGRLREEIE ELKRNQELLQ
760 770 780 790 800
SQLTEKDSMI ENMKSSQTSG TNEQSSAIVS ARDSEQVAEL KQELATLKSQ
810 820 830 840 850
LNSQSVEITK LQTEKQELLQ KTEAFAKSVE VQGETETIIA TKTTDVEGRL
860 870 880 890 900
SALLQETKEL KNEIKALSEE RTAIKEQLDS SNSTIAILQT EKDKLELEIT
910 920 930 940 950
DSKKEQDDLL VLLADQDQKI LSLKNKLKDL GHPVEEEDEL ESGDQEDEDD
960
ESEDPGKDLD HI
Length:962
Mass (Da):107,895
Last modified:March 6, 2007 - v2
Checksum:iC963652209031008
GO
Isoform 2 (identifier: O60763-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     98-98: V → VDDVE
     484-484: Q → QGDKIDRR

Show »
Length:973
Mass (Da):109,195
Checksum:i4DE9E8C5ED44514E
GO

Sequence cautioni

The sequence AAH06398 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75D → G in CAD89917 (PubMed:17974005).Curated1
Sequence conflicti85T → I in BAA25300 (PubMed:9478999).Curated1
Sequence conflicti93Missing in AAH32654 (PubMed:15489334).Curated1
Sequence conflicti248N → Y in CAD89917 (PubMed:17974005).Curated1
Sequence conflicti650N → D in CAD89917 (PubMed:17974005).Curated1
Sequence conflicti877Q → R in BAG36947 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03912098V → VDDVE in isoform 2. 1 Publication1
Alternative sequenceiVSP_039121484Q → QGDKIDRR in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86326 mRNA. Translation: BAA25300.1.
AK314289 mRNA. Translation: BAG36947.1.
AL832010 mRNA. Translation: CAD89917.1.
AC110615 Genomic DNA. No translation available.
AC104828 Genomic DNA. No translation available.
BC006398 mRNA. Translation: AAH06398.1. Sequence problems.
BC032654 mRNA. Translation: AAH32654.1.
CCDSiCCDS75144.1. [O60763-1]
CCDS77929.1. [O60763-2]
RefSeqiNP_001276978.1. NM_001290049.1. [O60763-2]
NP_003706.2. NM_003715.3. [O60763-1]
UniGeneiHs.744877.

Genome annotation databases

EnsembliENST00000264904; ENSP00000264904; ENSG00000138768. [O60763-2]
ENST00000514213; ENSP00000444850; ENSG00000138768. [O60763-1]
GeneIDi8615.
KEGGihsa:8615.
UCSCiuc003hiv.5. human. [O60763-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86326 mRNA. Translation: BAA25300.1.
AK314289 mRNA. Translation: BAG36947.1.
AL832010 mRNA. Translation: CAD89917.1.
AC110615 Genomic DNA. No translation available.
AC104828 Genomic DNA. No translation available.
BC006398 mRNA. Translation: AAH06398.1. Sequence problems.
BC032654 mRNA. Translation: AAH32654.1.
CCDSiCCDS75144.1. [O60763-1]
CCDS77929.1. [O60763-2]
RefSeqiNP_001276978.1. NM_001290049.1. [O60763-2]
NP_003706.2. NM_003715.3. [O60763-1]
UniGeneiHs.744877.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2W3CX-ray2.22A53-629[»]
ProteinModelPortaliO60763.
SMRiO60763.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114173. 67 interactors.
IntActiO60763. 25 interactors.
MINTiMINT-1136055.

PTM databases

iPTMnetiO60763.
PhosphoSitePlusiO60763.
SwissPalmiO60763.

Polymorphism and mutation databases

BioMutaiUSO1.

Proteomic databases

EPDiO60763.
MaxQBiO60763.
PeptideAtlasiO60763.
PRIDEiO60763.

Protocols and materials databases

DNASUi8615.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264904; ENSP00000264904; ENSG00000138768. [O60763-2]
ENST00000514213; ENSP00000444850; ENSG00000138768. [O60763-1]
GeneIDi8615.
KEGGihsa:8615.
UCSCiuc003hiv.5. human. [O60763-1]

Organism-specific databases

CTDi8615.
DisGeNETi8615.
GeneCardsiUSO1.
HGNCiHGNC:30904. USO1.
HPAiCAB010108.
HPA038282.
HPA038283.
MIMi603344. gene.
neXtProtiNX_O60763.
OpenTargetsiENSG00000138768.
PharmGKBiPA162408713.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00390000017018.
HOGENOMiHOG000016409.
HOVERGENiHBG018067.
InParanoidiO60763.
OMAiGYQVNQD.
OrthoDBiEOG091G046H.
PhylomeDBiO60763.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000138768-MONOMER.
ReactomeiR-HSA-162658. Golgi Cisternae Pericentriolar Stack Reorganization.
R-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-6807878. COPI-mediated anterograde transport.
SIGNORiO60763.

Miscellaneous databases

ChiTaRSiUSO1. human.
EvolutionaryTraceiO60763.
GeneWikiiUSO1.
GenomeRNAii8615.
PROiO60763.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000138768.
CleanExiHS_USO1.
ExpressionAtlasiO60763. baseline and differential.
GenevisibleiO60763. HS.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR006955. Uso1_p115_C.
IPR024095. Vesicle_P115-like.
IPR006953. Vesicle_Uso1_P115_head.
[Graphical view]
PANTHERiPTHR10013. PTHR10013. 1 hit.
PfamiPF04871. Uso1_p115_C. 1 hit.
PF04869. Uso1_p115_head. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUSO1_HUMAN
AccessioniPrimary (citable) accession number: O60763
Secondary accession number(s): B2RAQ0
, Q6PK63, Q86TB8, Q8N592
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 6, 2007
Last modified: November 2, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.