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O60759 (CYTIP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytohesin-interacting protein
Alternative name(s):
Cytohesin binder and regulator
Short name=CYBR
Cytohesin-associated scaffolding protein
Short name=CASP
Cytohesin-binding protein HE
Short name=Cbp HE
Pleckstrin homology Sec7 and coiled-coil domains-binding protein
Gene names
Name:CYTIP
Synonyms:PSCDBP
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

By its binding to cytohesin-1 (CYTH1), it modifies activation of ARFs by CYTH1 and its precise function may be to sequester CYTH1 in the cytoplasm.

Subunit structure

Interacts with CYTH1 and SNX27. Ref.1 Ref.2 Ref.6

Subcellular location

Cytoplasm. Early endosome. Note: Recruited from the cytosol to endosomes by SNX27. Ref.2 Ref.6

Tissue specificity

Expressed in lymph nodes, thymus, spleen, lung, peripheral blood leukocytes and bone marrow. Ref.1 Ref.2

Induction

By TNF and bacterial lipopolysaccharides (LPS). Ref.2

Sequence similarities

Contains 1 PDZ (DHR) domain.

Sequence caution

The sequence AAA16575.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
Endosome
   Coding sequence diversityPolymorphism
   DomainCoiled coil
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of cell adhesion

Inferred from direct assay Ref.2. Source: MGI

   Cellular_componentcell cortex

Inferred from direct assay Ref.2. Source: MGI

cytoplasm

Inferred from direct assay. Source: HPA

early endosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay. Source: HPA

   Molecular_functionprotein binding

Inferred from physical interaction Ref.1. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CYTH1Q154382EBI-997814,EBI-997830

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 359359Cytohesin-interacting protein
PRO_0000097061

Regions

Domain77 – 16690PDZ
Region166 – 18823Interaction with CYTH1
Coiled coil166 – 18823 Potential
Compositional bias241 – 32383Ser-rich

Natural variations

Natural variant371D → N. Ref.5
Corresponds to variant rs1042038 [ dbSNP | Ensembl ].
VAR_023534
Natural variant831Q → E.
Corresponds to variant rs2229345 [ dbSNP | Ensembl ].
VAR_051287
Natural variant3291P → S Found in a renal cell carcinoma case; somatic mutation. Ref.7
VAR_064706

Experimental info

Mutagenesis821K → E: No membrane-association. No change in the binding to CYTH1; when associated with A-90 and A-92. Ref.2
Mutagenesis901F → A: No membrane-association. No change in the binding to CYTH1; when associated with E-82 and A-92. Ref.2
Mutagenesis921I → A: No membrane-association. No change in the binding to CYTH1; when associated with E-82 and A-90. Ref.2
Sequence conflict2781T → Q in AAA16575. Ref.5
Sequence conflict339 – 3413KQL → RVA in AAA16575. Ref.5

Secondary structure

............... 359
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O60759 [UniParc].

Last modified September 27, 2005. Version 2.
Checksum: 76F0E0ED02EDD8C2

FASTA35940,010
        10         20         30         40         50         60 
MSLQRLLQHS SNGNLADFCA GPAYSSYSTL TGSLTMDDNR RIQMLADTVA TLPRGRKQLA 

        70         80         90        100        110        120 
LTRSSSLSDF SWSQRKLVTV EKQDNETFGF EIQSYRPQNQ NACSSEMFTL ICKIQEDSPA 

       130        140        150        160        170        180 
HCAGLQAGDV LANINGVSTE GFTYKQVVDL IRSSGNLLTI ETLNGTMILK RTELEAKLQV 

       190        200        210        220        230        240 
LKQTLKQKWV EYRSLQLQEH RLLHGDAANC PSLENMDLDE LSLFGPLPGP GPALVDRNRL 

       250        260        270        280        290        300 
SSESSCKSWL SSMTMDSEDG YQTCVSEDSS RGAFSRQTST DDECFIPKEG DDFLRRSSSR 

       310        320        330        340        350 
RNRSISNTSS GSMSPLWEGN LSSMFGTLPR KSRKGSVRKQ LLKFIPGLHR AVEEEESRF 

« Hide

References

« Hide 'large scale' references
[1]"Cybr, a cytokine-inducible protein that binds cytohesin-1 and regulates its activity."
Tang P., Cheng T.P., Agnello D., Wu C.-Y., Hissong B.D., Watford W.T., Ahn H.-J., Galon J., Moss J., Vaughan M., O'Shea J.J., Gadina M.
Proc. Natl. Acad. Sci. U.S.A. 99:2625-2629(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DOMAIN, INTERACTION WITH CYTH1.
[2]"Attenuation of cell adhesion in lymphocytes is regulated by CYTIP, a protein which mediates signal complex sequestration."
Boehm T., Hofer S., Winklehner P., Kellersch B., Geiger C., Trockenbacher A., Neyer S., Fiegl H., Ebner S., Ivarsson L., Schneider R., Kremmer E., Heufler C., Kolanus W.
EMBO J. 22:1014-1024(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INDUCTION, DOMAIN, TISSUE SPECIFICITY, INTERACTION WITH CYTH1, MUTAGENESIS OF LYS-82; PHE-90 AND ILE-92.
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[5]"Cloning a cDNA from human NK/T cells which codes for an unusual leucine zipper containing protein."
Dixon B., Sahely B., Liu L., Pohajdak B.
Biochim. Biophys. Acta 1216:321-324(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-359, VARIANT ASN-37.
Tissue: Blood.
[6]"Sorting nexin 27 interacts with the Cytohesin associated scaffolding protein (CASP) in lymphocytes."
MacNeil A.J., Mansour M., Pohajdak B.
Biochem. Biophys. Res. Commun. 359:848-853(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SNX27.
[7]"Exome sequencing identifies frequent mutation of the SWI/SNF complex gene PBRM1 in renal carcinoma."
Varela I., Tarpey P., Raine K., Huang D., Ong C.K., Stephens P., Davies H., Jones D., Lin M.L., Teague J., Bignell G., Butler A., Cho J., Dalgliesh G.L., Galappaththige D., Greenman C., Hardy C., Jia M. expand/collapse author list , Latimer C., Lau K.W., Marshall J., McLaren S., Menzies A., Mudie L., Stebbings L., Largaespada D.A., Wessels L.F.A., Richard S., Kahnoski R.J., Anema J., Tuveson D.A., Perez-Mancera P.A., Mustonen V., Fischer A., Adams D.J., Rust A., Chan-On W., Subimerb C., Dykema K., Furge K., Campbell P.J., Teh B.T., Stratton M.R., Futreal P.A.
Nature 469:539-542(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT SER-329.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF068836 mRNA. Translation: AAC19129.1.
AC019201 Genomic DNA. Translation: AAY14898.1.
BC036449 mRNA. Translation: AAH36449.1.
L06633 mRNA. Translation: AAA16575.1. Different initiation.
CCDSCCDS2204.1.
PIRS43424.
RefSeqNP_004279.3. NM_004288.4.
UniGeneHs.270.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2Z17X-ray2.70A67-163[»]
ProteinModelPortalO60759.
SMRO60759. Positions 70-163.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114961. 3 interactions.
IntActO60759. 4 interactions.
MINTMINT-6176803.
STRING9606.ENSP00000264192.

PTM databases

PhosphoSiteO60759.

Proteomic databases

MaxQBO60759.
PaxDbO60759.
PRIDEO60759.

Protocols and materials databases

DNASU9595.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000264192; ENSP00000264192; ENSG00000115165.
GeneID9595.
KEGGhsa:9595.
UCSCuc002tzj.1. human.

Organism-specific databases

CTD9595.
GeneCardsGC02M158271.
HGNCHGNC:9506. CYTIP.
HPAHPA007191.
MIM604448. gene.
neXtProtNX_O60759.
PharmGKBPA164718652.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG244814.
HOGENOMHOG000231871.
HOVERGENHBG053061.
InParanoidO60759.
OMAEMCTLIC.
OrthoDBEOG72NRQP.
PhylomeDBO60759.
TreeFamTF316315.

Gene expression databases

ArrayExpressO60759.
BgeeO60759.
CleanExHS_CYTIP.
GenevestigatorO60759.

Family and domain databases

Gene3D2.30.42.10. 1 hit.
InterProIPR001478. PDZ.
[Graphical view]
PfamPF00595. PDZ. 1 hit.
[Graphical view]
SMARTSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMSSF50156. SSF50156. 1 hit.
PROSITEPS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO60759.
GeneWikiPSCDBP.
GenomeRNAi9595.
NextBio36003.
PROO60759.
SOURCESearch...

Entry information

Entry nameCYTIP_HUMAN
AccessionPrimary (citable) accession number: O60759
Secondary accession number(s): Q15630, Q8NE32
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: September 27, 2005
Last modified: July 9, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM