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O60759

- CYTIP_HUMAN

UniProt

O60759 - CYTIP_HUMAN

Protein

Cytohesin-interacting protein

Gene

CYTIP

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 2 (27 Sep 2005)
      Previous versions | rss
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    Functioni

    By its binding to cytohesin-1 (CYTH1), it modifies activation of ARFs by CYTH1 and its precise function may be to sequester CYTH1 in the cytoplasm.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. regulation of cell adhesion Source: MGI

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytohesin-interacting protein
    Alternative name(s):
    Cytohesin binder and regulator
    Short name:
    CYBR
    Cytohesin-associated scaffolding protein
    Short name:
    CASP
    Cytohesin-binding protein HE
    Short name:
    Cbp HE
    Pleckstrin homology Sec7 and coiled-coil domains-binding protein
    Gene namesi
    Name:CYTIP
    Synonyms:PSCDBP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:9506. CYTIP.

    Subcellular locationi

    Cytoplasm. Early endosome
    Note: Recruited from the cytosol to endosomes by SNX27.

    GO - Cellular componenti

    1. cell cortex Source: MGI
    2. cytoplasm Source: HPA
    3. early endosome Source: UniProtKB-SubCell
    4. nucleus Source: HPA

    Keywords - Cellular componenti

    Cytoplasm, Endosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi82 – 821K → E: No membrane-association. No change in the binding to CYTH1; when associated with A-90 and A-92. 1 Publication
    Mutagenesisi90 – 901F → A: No membrane-association. No change in the binding to CYTH1; when associated with E-82 and A-92. 1 Publication
    Mutagenesisi92 – 921I → A: No membrane-association. No change in the binding to CYTH1; when associated with E-82 and A-90. 1 Publication

    Organism-specific databases

    PharmGKBiPA164718652.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 359359Cytohesin-interacting proteinPRO_0000097061Add
    BLAST

    Proteomic databases

    MaxQBiO60759.
    PaxDbiO60759.
    PRIDEiO60759.

    PTM databases

    PhosphoSiteiO60759.

    Expressioni

    Tissue specificityi

    Expressed in lymph nodes, thymus, spleen, lung, peripheral blood leukocytes and bone marrow.2 Publications

    Inductioni

    By TNF and bacterial lipopolysaccharides (LPS).1 Publication

    Gene expression databases

    ArrayExpressiO60759.
    BgeeiO60759.
    CleanExiHS_CYTIP.
    GenevestigatoriO60759.

    Organism-specific databases

    HPAiHPA007191.

    Interactioni

    Subunit structurei

    Interacts with CYTH1 and SNX27.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CYTH1Q154382EBI-997814,EBI-997830

    Protein-protein interaction databases

    BioGridi114961. 3 interactions.
    IntActiO60759. 4 interactions.
    MINTiMINT-6176803.
    STRINGi9606.ENSP00000264192.

    Structurei

    Secondary structure

    1
    359
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi77 – 815
    Beta strandi89 – 946
    Beta strandi101 – 1033
    Beta strandi109 – 1146
    Helixi119 – 1235
    Helixi144 – 15310
    Turni154 – 1563
    Beta strandi157 – 1615

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2Z17X-ray2.70A67-163[»]
    ProteinModelPortaliO60759.
    SMRiO60759. Positions 70-163.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO60759.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini77 – 16690PDZPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni166 – 18823Interaction with CYTH1Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili166 – 18823Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi241 – 32383Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG244814.
    HOGENOMiHOG000231871.
    HOVERGENiHBG053061.
    InParanoidiO60759.
    OMAiEMCTLIC.
    OrthoDBiEOG72NRQP.
    PhylomeDBiO60759.
    TreeFamiTF316315.

    Family and domain databases

    Gene3Di2.30.42.10. 1 hit.
    InterProiIPR001478. PDZ.
    [Graphical view]
    PfamiPF00595. PDZ. 1 hit.
    [Graphical view]
    SMARTiSM00228. PDZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF50156. SSF50156. 1 hit.
    PROSITEiPS50106. PDZ. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O60759-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLQRLLQHS SNGNLADFCA GPAYSSYSTL TGSLTMDDNR RIQMLADTVA    50
    TLPRGRKQLA LTRSSSLSDF SWSQRKLVTV EKQDNETFGF EIQSYRPQNQ 100
    NACSSEMFTL ICKIQEDSPA HCAGLQAGDV LANINGVSTE GFTYKQVVDL 150
    IRSSGNLLTI ETLNGTMILK RTELEAKLQV LKQTLKQKWV EYRSLQLQEH 200
    RLLHGDAANC PSLENMDLDE LSLFGPLPGP GPALVDRNRL SSESSCKSWL 250
    SSMTMDSEDG YQTCVSEDSS RGAFSRQTST DDECFIPKEG DDFLRRSSSR 300
    RNRSISNTSS GSMSPLWEGN LSSMFGTLPR KSRKGSVRKQ LLKFIPGLHR 350
    AVEEEESRF 359
    Length:359
    Mass (Da):40,010
    Last modified:September 27, 2005 - v2
    Checksum:i76F0E0ED02EDD8C2
    GO
    Isoform 2 (identifier: O60759-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-106: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:253
    Mass (Da):28,172
    Checksum:iCE3E6B4FB0C6FA26
    GO

    Sequence cautioni

    The sequence AAA16575.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti278 – 2781T → Q in AAA16575. (PubMed:8241278)Curated
    Sequence conflicti339 – 3413KQL → RVA in AAA16575. (PubMed:8241278)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti37 – 371D → N.1 Publication
    Corresponds to variant rs1042038 [ dbSNP | Ensembl ].
    VAR_023534
    Natural varianti83 – 831Q → E.
    Corresponds to variant rs2229345 [ dbSNP | Ensembl ].
    VAR_051287
    Natural varianti329 – 3291P → S Found in a renal cell carcinoma case; somatic mutation. 1 Publication
    VAR_064706

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 106106Missing in isoform 2. 1 PublicationVSP_055502Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF068836 mRNA. Translation: AAC19129.1.
    AK301544 mRNA. Translation: BAG63041.1.
    AC019201 Genomic DNA. Translation: AAY14898.1.
    BC036449 mRNA. Translation: AAH36449.1.
    L06633 mRNA. Translation: AAA16575.1. Different initiation.
    CCDSiCCDS2204.1.
    PIRiS43424.
    RefSeqiNP_004279.3. NM_004288.4.
    UniGeneiHs.270.

    Genome annotation databases

    EnsembliENST00000264192; ENSP00000264192; ENSG00000115165. [O60759-1]
    GeneIDi9595.
    KEGGihsa:9595.
    UCSCiuc002tzj.1. human.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF068836 mRNA. Translation: AAC19129.1 .
    AK301544 mRNA. Translation: BAG63041.1 .
    AC019201 Genomic DNA. Translation: AAY14898.1 .
    BC036449 mRNA. Translation: AAH36449.1 .
    L06633 mRNA. Translation: AAA16575.1 . Different initiation.
    CCDSi CCDS2204.1.
    PIRi S43424.
    RefSeqi NP_004279.3. NM_004288.4.
    UniGenei Hs.270.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2Z17 X-ray 2.70 A 67-163 [» ]
    ProteinModelPortali O60759.
    SMRi O60759. Positions 70-163.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114961. 3 interactions.
    IntActi O60759. 4 interactions.
    MINTi MINT-6176803.
    STRINGi 9606.ENSP00000264192.

    PTM databases

    PhosphoSitei O60759.

    Proteomic databases

    MaxQBi O60759.
    PaxDbi O60759.
    PRIDEi O60759.

    Protocols and materials databases

    DNASUi 9595.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000264192 ; ENSP00000264192 ; ENSG00000115165 . [O60759-1 ]
    GeneIDi 9595.
    KEGGi hsa:9595.
    UCSCi uc002tzj.1. human.

    Organism-specific databases

    CTDi 9595.
    GeneCardsi GC02M158271.
    HGNCi HGNC:9506. CYTIP.
    HPAi HPA007191.
    MIMi 604448. gene.
    neXtProti NX_O60759.
    PharmGKBi PA164718652.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG244814.
    HOGENOMi HOG000231871.
    HOVERGENi HBG053061.
    InParanoidi O60759.
    OMAi EMCTLIC.
    OrthoDBi EOG72NRQP.
    PhylomeDBi O60759.
    TreeFami TF316315.

    Miscellaneous databases

    EvolutionaryTracei O60759.
    GeneWikii PSCDBP.
    GenomeRNAii 9595.
    NextBioi 36003.
    PROi O60759.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O60759.
    Bgeei O60759.
    CleanExi HS_CYTIP.
    Genevestigatori O60759.

    Family and domain databases

    Gene3Di 2.30.42.10. 1 hit.
    InterProi IPR001478. PDZ.
    [Graphical view ]
    Pfami PF00595. PDZ. 1 hit.
    [Graphical view ]
    SMARTi SM00228. PDZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50156. SSF50156. 1 hit.
    PROSITEi PS50106. PDZ. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DOMAIN, INTERACTION WITH CYTH1.
    2. "Attenuation of cell adhesion in lymphocytes is regulated by CYTIP, a protein which mediates signal complex sequestration."
      Boehm T., Hofer S., Winklehner P., Kellersch B., Geiger C., Trockenbacher A., Neyer S., Fiegl H., Ebner S., Ivarsson L., Schneider R., Kremmer E., Heufler C., Kolanus W.
      EMBO J. 22:1014-1024(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, INDUCTION, DOMAIN, TISSUE SPECIFICITY, INTERACTION WITH CYTH1, MUTAGENESIS OF LYS-82; PHE-90 AND ILE-92.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Mammary gland.
    4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Testis.
    6. "Cloning a cDNA from human NK/T cells which codes for an unusual leucine zipper containing protein."
      Dixon B., Sahely B., Liu L., Pohajdak B.
      Biochim. Biophys. Acta 1216:321-324(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-359 (ISOFORM 1), VARIANT ASN-37.
      Tissue: Blood.
    7. "Sorting nexin 27 interacts with the Cytohesin associated scaffolding protein (CASP) in lymphocytes."
      MacNeil A.J., Mansour M., Pohajdak B.
      Biochem. Biophys. Res. Commun. 359:848-853(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SNX27.
    8. Cited for: VARIANT SER-329.

    Entry informationi

    Entry nameiCYTIP_HUMAN
    AccessioniPrimary (citable) accession number: O60759
    Secondary accession number(s): B4DWH9, Q15630, Q8NE32
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2005
    Last sequence update: September 27, 2005
    Last modified: October 1, 2014
    This is version 106 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3