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Reviewed, UniProtKB/Swiss-Prot O60741 (HCN1_HUMAN)

Last modified May 26, 2009. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Alternative name(s):
    Brain cyclic nucleotide-gated channel 1
      Short name=BCNG-1
Gene names
Name: HCN1
Synonyms: BCNG1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length890 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli.

Subunit structure

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN2 By similarity. Interacts with KCNE2 By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas. Ref.2

Domain

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similarities

Belongs to the potassium channel HCN family.

Contains 1 cyclic nucleotide-binding domain.

Sequence caution

The sequence AAC39759.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 890890Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
PRO_0000054107

Regions

Topological domain1 – 146146Cytoplasmic Potential
Transmembrane147 – 16721Segment S1 Potential
Transmembrane174 – 19421Segment S2 Potential
Topological domain195 – 21925Cytoplasmic Potential
Transmembrane220 – 24021Segment S3 Potential
Transmembrane249 – 26921Segment S4 Potential
Topological domain270 – 30031Cytoplasmic Potential
Transmembrane301 – 32121Segment S5 Potential
Transmembrane345 – 36622Segment H5 (pore-forming) Potential
Transmembrane372 – 39221Segment S6 Potential
Topological domain393 – 890498Cytoplasmic Potential
Nucleotide binding475 – 592118cAMP
Region89 – 14052Involved in subunit assembly By similarity
Compositional bias1 – 8080Gly-rich
Compositional bias726 – 75631Gln-rich
Compositional bias857 – 8637Poly-Pro

Amino acid modifications

Modified residue411Phosphothreonine By similarity
Glycosylation3381N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict7861L → F in AAC39759. Ref.2
Sequence conflict7891S → W in AAC39759. Ref.2
Sequence conflict8411L → F in AAC39759. Ref.2
Sequence conflict8571P → L in AAC39759. Ref.2
Sequence conflict8611P → L in AAC39759. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O60741-1 [UniParc].

Last modified May 5, 2009. Version 3.
Checksum: A62F5E79C01257A2

FASTA89098,796
        10         20         30         40         50         60 
MEGGGKPNSS SNSRDDGNSV FPAKASATGA GPAAAEKRLG TPPGGGGAGA KEHGNSVCFK 

        70         80         90        100        110        120 
VDGGGGGGGG GGGGEEPAGG FEDAEGPRRQ YGFMQRQFTS MLQPGVNKFS LRMFGSQKAV 

       130        140        150        160        170        180 
EKEQERVKTA GFWIIHPYSD FRFYWDLIML IMMVGNLVII PVGITFFTEQ TTTPWIIFNV 

       190        200        210        220        230        240 
ASDTVFLLDL IMNFRTGTVN EDSSEIILDP KVIKMNYLKS WFVVDFISSI PVDYIFLIVE 

       250        260        270        280        290        300 
KGMDSEVYKT ARALRIVRFT KILSLLRLLR LSRLIRYIHQ WEEIFHMTYD LASAVVRIFN 

       310        320        330        340        350        360 
LIGMMLLLCH WDGCLQFLVP LLQDFPPDCW VSLNEMVNDS WGKQYSYALF KAMSHMLCIG 

       370        380        390        400        410        420 
YGAQAPVSMS DLWITMLSMI VGATCYAMFV GHATALIQSL DSSRRQYQEK YKQVEQYMSF 

       430        440        450        460        470        480 
HKLPADMRQK IHDYYEHRYQ GKIFDEENIL NELNDPLREE IVNFNCRKLV ATMPLFANAD 

       490        500        510        520        530        540 
PNFVTAMLSK LRFEVFQPGD YIIREGAVGK KMYFIQHGVA GVITKSSKEM KLTDGSYFGE 

       550        560        570        580        590        600 
ICLLTKGRRT ASVRADTYCR LYSLSVDNFN EVLEEYPMMR RAFETVAIDR LDRIGKKNSI 

       610        620        630        640        650        660 
LLQKFQKDLN TGVFNNQENE ILKQIVKHDR EMVQAIAPIN YPQMTTLNST SSTTTPTSRM 

       670        680        690        700        710        720 
RTQSPPVYTA TSLSHSNLHS PSPSTQTPQP SAILSPCSYT TAVCSPPVQS PLAARTFHYA 

       730        740        750        760        770        780 
SPTASQLSLM QQQPQQQVQQ SQPPQTQPQQ PSPQPQTPGS STPKNEVHKS TQALHNTNLT 

       790        800        810        820        830        840 
REVRPLSASQ PSLPHEVSTL ISRPHPTVGE SLASIPQPVT AVPGTGLQAG GRSTVPQRVT 

       850        860        870        880        890 
LFRQMSSGAI PPNRGVPPAP PPPAAALPRE SSSVLNTDPD AEKPRFASNL 

« Hide

References

« Hide 'large scale' references
[1]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed: 15372022] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Identification of a gene encoding a hyperpolarization-activated 'pacemaker' channel of brain."
Santoro B., Liu D.T., Yao H., Bartsch D., Kandel E.R., Siegelbaum S.A., Tibbs G.R.
Cell 93:717-729(1998) [PubMed: 9630217] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-862, TISSUE SPECIFICITY.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

AC099514 Genomic DNA. No translation available.
AC114975 Genomic DNA. No translation available.
AC117529 Genomic DNA. No translation available.
AC138520 Genomic DNA. No translation available.
AF064876 mRNA. Translation: AAC39759.1. Sequence problems.
IPIIPI00031506.
RefSeqNP_066550.2.
UniGeneHs.650434

3D structure databases

SMRO60741. Positions 401-601.
ModBaseSearch...

PTM databases

PhosphoSiteO60741.

Proteomic databases

PRIDEO60741.

Genome annotation databases

EnsemblENSG00000164588. Homo sapiens. [Contig view]
GeneID348980.
KEGGhsa:348980.

Organism-specific databases

GeneCardsGC05M045297.
HGNCHGNC:4845. HCN1.
MIM602780. gene.
PharmGKBPA77.
GenAtlasSearch...

Phylogenomic databases

HOGENOMO60741.
HOVERGENO60741.

Gene expression databases

ArrayExpressO60741.
BgeeO60741.
CleanExHS_HCN1.
GermOnlineENSG00000164588. Homo sapiens.

Family and domain databases

InterProIPR000595. cNMP_bd.
IPR018488. cNMP_bd_CS.
IPR005821. Ion_trans.
IPR013621. Ion_trans_N.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
Gene3DG3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
PfamPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF08412. Ion_trans_N. 1 hit.
[Graphical view]
PRINTSPR01463. EAGCHANLFMLY.
SMARTSM00100. cNMP. 1 hit.
[Graphical view]
PROSITEPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. False negative.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

Entry information

Entry nameHCN1_HUMAN
AccessionPrimary (citable) accession number: O60741
Entry history
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: May 5, 2009
Last modified: May 26, 2009
This is version 71 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents