ID   MAGC1_HUMAN             Reviewed;        1142 AA.
AC   O60732; A0PK03; O75451; Q8TCV4;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   27-JUN-2006, sequence version 3.
DT   24-JAN-2024, entry version 160.
DE   RecName: Full=Melanoma-associated antigen C1;
DE   AltName: Full=Cancer/testis antigen 7.1;
DE            Short=CT7.1;
DE   AltName: Full=MAGE-C1 antigen;
GN   Name=MAGEC1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS TYR-25; ILE-151 AND
RP   GLN-327.
RC   TISSUE=Melanoma;
RX   PubMed=9485030;
RA   Lucas S., De Smet C., Arden K.C., Viars C.S., Lethe B.G., Lurquin C.,
RA   Boon T.;
RT   "Identification of a new MAGE gene with tumor-specific expression by
RT   representational difference analysis.";
RL   Cancer Res. 58:743-752(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND VARIANTS ILE-151 AND GLN-327.
RC   TISSUE=Melanoma;
RX   PubMed=9618514; DOI=10.1073/pnas.95.12.6919;
RA   Chen Y.-T., Gure A.O., Tsang S., Stockert E., Jager E., Knuth A., Old L.J.;
RT   "Identification of multiple cancer/testis antigens by allogeneic antibody
RT   screening of a melanoma cell line library.";
RL   Proc. Natl. Acad. Sci. U.S.A. 95:6919-6923(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15772651; DOI=10.1038/nature03440;
RA   Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA   Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA   Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA   Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA   Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA   Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA   Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA   Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA   Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA   Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA   Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA   Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA   Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA   Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA   Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA   Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA   Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA   Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA   Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA   Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA   Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA   Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA   Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA   Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA   Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA   Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA   Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA   Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA   Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA   Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA   McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA   Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA   Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA   Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA   Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA   Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA   Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA   Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA   Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA   Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA   d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA   Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA   Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA   Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA   Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA   Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA   Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA   Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA   Rogers J., Bentley D.R.;
RT   "The DNA sequence of the human X chromosome.";
RL   Nature 434:325-337(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63; SER-207; SER-382 AND
RP   SER-1063, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Erythroleukemia;
RX   PubMed=23186163; DOI=10.1021/pr300630k;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
CC   -!- INTERACTION:
CC       O60732; P78358: CTAG1B; NbExp=6; IntAct=EBI-1188463, EBI-1188472;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=O60732-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=O60732-2; Sequence=VSP_056382;
CC   -!- TISSUE SPECIFICITY: Expressed in testis and in tumors of a wide variety
CC       of histologic types.
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DR   EMBL; AF064589; AAC18837.1; -; Genomic_DNA.
DR   EMBL; AF056334; AAC24227.1; -; mRNA.
DR   EMBL; AL022152; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC127771; AAI27772.1; -; mRNA.
DR   CCDS; CCDS35417.1; -. [O60732-1]
DR   RefSeq; NP_005453.2; NM_005462.4. [O60732-1]
DR   RefSeq; XP_011529720.1; XM_011531418.2. [O60732-1]
DR   AlphaFoldDB; O60732; -.
DR   SMR; O60732; -.
DR   BioGRID; 115272; 17.
DR   IntAct; O60732; 9.
DR   MINT; O60732; -.
DR   STRING; 9606.ENSP00000285879; -.
DR   GlyGen; O60732; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; O60732; -.
DR   PhosphoSitePlus; O60732; -.
DR   BioMuta; MAGEC1; -.
DR   EPD; O60732; -.
DR   MassIVE; O60732; -.
DR   MaxQB; O60732; -.
DR   PaxDb; 9606-ENSP00000285879; -.
DR   PeptideAtlas; O60732; -.
DR   ProteomicsDB; 49577; -. [O60732-1]
DR   ProteomicsDB; 77; -.
DR   Pumba; O60732; -.
DR   Antibodypedia; 528; 171 antibodies from 27 providers.
DR   DNASU; 9947; -.
DR   Ensembl; ENST00000285879.5; ENSP00000285879.4; ENSG00000155495.9. [O60732-1]
DR   Ensembl; ENST00000406005.2; ENSP00000385500.2; ENSG00000155495.9. [O60732-2]
DR   GeneID; 9947; -.
DR   KEGG; hsa:9947; -.
DR   MANE-Select; ENST00000285879.5; ENSP00000285879.4; NM_005462.5; NP_005453.2.
DR   UCSC; uc004fbt.4; human. [O60732-1]
DR   AGR; HGNC:6812; -.
DR   CTD; 9947; -.
DR   DisGeNET; 9947; -.
DR   GeneCards; MAGEC1; -.
DR   HGNC; HGNC:6812; MAGEC1.
DR   HPA; ENSG00000155495; Tissue enriched (testis).
DR   MIM; 300223; gene.
DR   neXtProt; NX_O60732; -.
DR   OpenTargets; ENSG00000155495; -.
DR   PharmGKB; PA30558; -.
DR   VEuPathDB; HostDB:ENSG00000155495; -.
DR   eggNOG; KOG4562; Eukaryota.
DR   GeneTree; ENSGT00940000164535; -.
DR   HOGENOM; CLU_277023_0_0_1; -.
DR   InParanoid; O60732; -.
DR   OMA; HSQYPQC; -.
DR   OrthoDB; 4706401at2759; -.
DR   PhylomeDB; O60732; -.
DR   TreeFam; TF328505; -.
DR   PathwayCommons; O60732; -.
DR   SignaLink; O60732; -.
DR   BioGRID-ORCS; 9947; 12 hits in 775 CRISPR screens.
DR   GenomeRNAi; 9947; -.
DR   Pharos; O60732; Tbio.
DR   PRO; PR:O60732; -.
DR   Proteomes; UP000005640; Chromosome X.
DR   RNAct; O60732; Protein.
DR   Bgee; ENSG00000155495; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 18 other cell types or tissues.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 1.10.10.1200; MAGE homology domain, winged helix WH1 motif; 1.
DR   Gene3D; 1.10.10.1210; MAGE homology domain, winged helix WH2 motif; 1.
DR   InterPro; IPR037445; MAGE.
DR   InterPro; IPR041898; MAGE_WH1.
DR   InterPro; IPR041899; MAGE_WH2.
DR   InterPro; IPR002190; MHD_dom.
DR   PANTHER; PTHR11736:SF17; MELANOMA-ASSOCIATED ANTIGEN C1; 1.
DR   PANTHER; PTHR11736; MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN; 1.
DR   Pfam; PF01454; MAGE; 1.
DR   SMART; SM01373; MAGE; 1.
DR   PROSITE; PS50838; MAGE; 1.
DR   Genevisible; O60732; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Phosphoprotein; Reference proteome; Repeat;
KW   Tumor antigen.
FT   CHAIN           1..1142
FT                   /note="Melanoma-associated antigen C1"
FT                   /id="PRO_0000156720"
FT   DOMAIN          908..1106
FT                   /note="MAGE"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00127"
FT   REGION          1..132
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          502..778
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          791..893
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1118..1142
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        11..61
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        70..132
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        502..532
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        547..655
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        663..713
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        732..759
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        809..893
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1119..1142
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         63
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         207
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         382
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         1063
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   VAR_SEQ         1..933
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_056382"
FT   VARIANT         25
FT                   /note="C -> Y (in dbSNP:rs176036)"
FT                   /evidence="ECO:0000269|PubMed:9485030"
FT                   /id="VAR_053501"
FT   VARIANT         151
FT                   /note="T -> I (in dbSNP:rs176037)"
FT                   /evidence="ECO:0000269|PubMed:9485030,
FT                   ECO:0000269|PubMed:9618514"
FT                   /id="VAR_053502"
FT   VARIANT         257
FT                   /note="Q -> H (in dbSNP:rs143440588)"
FT                   /id="VAR_053503"
FT   VARIANT         276
FT                   /note="F -> S (in dbSNP:rs75148863)"
FT                   /id="VAR_053504"
FT   VARIANT         327
FT                   /note="H -> Q (in dbSNP:rs176047)"
FT                   /evidence="ECO:0000269|PubMed:9485030,
FT                   ECO:0000269|PubMed:9618514"
FT                   /id="VAR_060068"
FT   VARIANT         709
FT                   /note="H -> Y (in dbSNP:rs56256227)"
FT                   /id="VAR_062121"
FT   CONFLICT        221..225
FT                   /note="TQSTF -> SQRTS (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        239
FT                   /note="P -> S (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        252..253
FT                   /note="FS -> SP (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        264
FT                   /note="A -> P (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        267
FT                   /note="S -> P (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        274
FT                   /note="P -> R (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        281..283
FT                   /note="VSL -> LSI (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        299
FT                   /note="P -> A (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        309..311
FT                   /note="SSS -> PSF (in Ref. 2; AAC24227)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        316..318
FT                   /note="LSL -> VSI (in Ref. 2; AAC24227)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        337
FT                   /note="L -> P (in Ref. 2; AAC24227)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        342..343
FT                   /note="MT -> VS (in Ref. 2; AAC24227)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        353
FT                   /note="I -> L (in Ref. 2; AAC24227)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        360..361
FT                   /note="SA -> RT (in Ref. 2; AAC24227)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        467
FT                   /note="H -> Q (in Ref. 1; AAC18837)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1142 AA;  123643 MW;  6C9AE820156F3C50 CRC64;
     MGDKDMPTAG MPSLLQSSSE SPQSCPEGED SQSPLQIPQS SPESDDTLYP LQSPQSRSEG
     EDSSDPLQRP PEGKDSQSPL QIPQSSPEGD DTQSPLQNSQ SSPEGKDSLS PLEISQSPPE
     GEDVQSPLQN PASSFFSSAL LSIFQSSPES TQSPFEGFPQ SVLQIPVSAA SSSTLVSIFQ
     SSPESTQSPF EGFPQSPLQI PVSRSFSSTL LSIFQSSPER TQSTFEGFAQ SPLQIPVSPS
     SSSTLLSLFQ SFSERTQSTF EGFAQSSLQI PVSPSFSSTL VSLFQSSPER TQSTFEGFPQ
     SPLQIPVSSS SSSTLLSLFQ SSPERTHSTF EGFPQSLLQI PMTSSFSSTL LSIFQSSPES
     AQSTFEGFPQ SPLQIPGSPS FSSTLLSLFQ SSPERTHSTF EGFPQSPLQI PMTSSFSSTL
     LSILQSSPES AQSAFEGFPQ SPLQIPVSSS FSYTLLSLFQ SSPERTHSTF EGFPQSPLQI
     PVSSSSSSST LLSLFQSSPE CTQSTFEGFP QSPLQIPQSP PEGENTHSPL QIVPSLPEWE
     DSLSPHYFPQ SPPQGEDSLS PHYFPQSPPQ GEDSLSPHYF PQSPQGEDSL SPHYFPQSPP
     QGEDSMSPLY FPQSPLQGEE FQSSLQSPVS ICSSSTPSSL PQSFPESSQS PPEGPVQSPL
     HSPQSPPEGM HSQSPLQSPE SAPEGEDSLS PLQIPQSPLE GEDSLSSLHF PQSPPEWEDS
     LSPLHFPQFP PQGEDFQSSL QSPVSICSSS TSLSLPQSFP ESPQSPPEGP AQSPLQRPVS
     SFFSYTLASL LQSSHESPQS PPEGPAQSPL QSPVSSFPSS TSSSLSQSSP VSSFPSSTSS
     SLSKSSPESP LQSPVISFSS STSLSPFSEE SSSPVDEYTS SSDTLLESDS LTDSESLIES
     EPLFTYTLDE KVDELARFLL LKYQVKQPIT KAEMLTNVIS RYTGYFPVIF RKAREFIEIL
     FGISLREVDP DDSYVFVNTL DLTSEGCLSD EQGMSQNRLL ILILSIIFIK GTYASEEVIW
     DVLSGIGVRA GREHFAFGEP RELLTKVWVQ EHYLEYREVP NSSPPRYEFL WGPRAHSEVI
     KRKVVEFLAM LKNTVPITFP SSYKDALKDV EERAQAIIDT TDDSTATESA SSSVMSPSFS
     SE
//