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Reviewed, UniProtKB/Swiss-Prot O60725 (ICMT_HUMAN)

Last modified June 16, 2009. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Protein-S-isoprenylcysteine O-methyltransferase
    EC=2.1.1.100
Alternative name(s):
    Isoprenylcysteine carboxylmethyltransferase
    Prenylcysteine carboxyl methyltransferase
      Short name=pcCMT
    Prenylated protein carboxyl methyltransferase
      Short name=PPMT
Gene names
Name: ICMT
Synonyms: PCCMT
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length284 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.

Catalytic activity

S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.

Cofactor

Divalent cations. Probably zinc By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Tissue specificity

Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons. Ref.3

Sequence similarities

Belongs to the isoprenylcysteine O-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 284284Protein-S-isoprenylcysteine O-methyltransferase
PRO_0000209894

Regions

Transmembrane18 – 3821 Potential
Transmembrane42 – 6221 Potential
Transmembrane66 – 8621 Potential
Transmembrane214 – 23421 Potential

Sequences

Sequence LengthMass (Da)Tools
O60725-1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: C86741B13ACA611C

FASTA28431,938
        10         20         30         40         50         60 
MAGCAARAPP GSEARLSLAT FLLGASVLAL PLLTRAGLQG RTGLALYVAG LNALLLLLYR 

        70         80         90        100        110        120 
PPRYQIAIRA CFLGFVFGCG TLLSFSQSSW SHFGWYMCSL SLFHYSEYLV TAVNNPKSLS 

       130        140        150        160        170        180 
LDSFLLNHSL EYTVAALSSW LEFTLENIFW PELKQITWLS VTGLLMVVFG ECLRKAAMFT 

       190        200        210        220        230        240 
AGSNFNHVVQ NEKSDTHTLV TSGVYAWFRH PSYVGWFYWS IGTQVMLCNP ICGVSYALTV 

       250        260        270        280 
WRFFRDRTEE EEISLIHFFG EEYLEYKKRV PTGLPFIKGV KVDL 

« Hide

References

« Hide 'large scale' references
[1]"Mammalian prenylcysteine carboxyl methyltransferase is in the endoplasmic reticulum."
Dai Q., Choy E., Chiu V., Romano J., Slivka S.R., Steitz S.A., Michaelis S., Philips M.R.
J. Biol. Chem. 273:15030-15034(1998) [PubMed: 9614111] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
Tissue: Myeloid.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Polyglutamine expansion down-regulates specific neuronal genes before pathologic changes in SCA1."
Lin X., Antalffy B., Kang D., Orr H.T., Zoghbi H.Y.
Nat. Neurosci. 3:157-163(2000) [PubMed: 10649571] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

AF064084 mRNA. Translation: AAC16554.1.
BC028168 mRNA. Translation: AAH28168.1.
IPIIPI00031458.
RefSeqNP_036537.1.
UniGeneHs.515688
Hs.562083

3D structure databases

ModBaseSearch...

Proteomic databases

PRIDEO60725.

Genome annotation databases

EnsemblENSG00000116237. Homo sapiens. [Contig view]
GeneID23463.
KEGGhsa:23463.

Organism-specific databases

GeneCardsGC01M006215.
H-InvDBHIX0000076.
HGNCHGNC:5350. ICMT.
MIM605851. gene.
PharmGKBPA29598.
GenAtlasSearch...

Phylogenomic databases

HOGENOMO60725.
HOVERGENO60725.
OMAO60725. ELKQITW.

Enzyme and pathway databases

BRENDA2.1.1.100. 247.

Gene expression databases

ArrayExpressO60725.
BgeeO60725.
CleanExHS_ICMT.
GermOnlineENSG00000116237. Homo sapiens.

Family and domain databases

InterProIPR007269. ICMT_MeTrfase.
[Graphical view]
PfamPF04140. ICMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio45777.
SOURCESearch...

Entry information

Entry nameICMT_HUMAN
AccessionPrimary (citable) accession number: O60725
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: August 1, 1998
Last modified: June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents