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Protein

Protein-S-isoprenylcysteine O-methyltransferase

Gene

ICMT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.

Catalytic activityi

S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Divalent metal cations. Probably Zn2+.By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular protein modification process Source: ProtInc
  • C-terminal protein methylation Source: ProtInc
  • post-translational protein modification Source: Reactome
  • protein targeting to membrane Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciZFISH:HS03999-MONOMER.
ReactomeiR-HSA-163841. Gamma carboxylation, hypusine formation and arylsulfatase activation.
SABIO-RKO60725.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100)
Alternative name(s):
Isoprenylcysteine carboxylmethyltransferase
Prenylated protein carboxyl methyltransferase
Short name:
PPMT
Prenylcysteine carboxyl methyltransferase
Short name:
pcCMT
Gene namesi
Name:ICMT
Synonyms:PCCMT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:5350. ICMT.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16CytoplasmicSequence analysisAdd BLAST16
Transmembranei17 – 33HelicalSequence analysisAdd BLAST17
Topological domaini34 – 41LumenalSequence analysis8
Transmembranei42 – 59HelicalSequence analysisAdd BLAST18
Topological domaini60 – 69CytoplasmicSequence analysis10
Transmembranei70 – 87HelicalSequence analysisAdd BLAST18
Topological domaini88 – 92LumenalSequence analysis5
Transmembranei93 – 112HelicalSequence analysisAdd BLAST20
Topological domaini113 – 131CytoplasmicSequence analysisAdd BLAST19
Transmembranei132 – 149HelicalSequence analysisAdd BLAST18
Topological domaini150 – 154LumenalSequence analysis5
Transmembranei155 – 174HelicalSequence analysisAdd BLAST20
Topological domaini175 – 212CytoplasmicSequence analysisAdd BLAST38
Transmembranei213 – 228HelicalSequence analysisAdd BLAST16
Topological domaini229LumenalSequence analysis1
Transmembranei230 – 244HelicalSequence analysisAdd BLAST15
Topological domaini245 – 284CytoplasmicSequence analysisAdd BLAST40

GO - Cellular componenti

  • endoplasmic reticulum Source: MGI
  • endoplasmic reticulum membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi23463.
OpenTargetsiENSG00000116237.
PharmGKBiPA29598.

Chemistry databases

ChEMBLiCHEMBL4699.

Polymorphism and mutation databases

BioMutaiICMT.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002098941 – 284Protein-S-isoprenylcysteine O-methyltransferaseAdd BLAST284

Proteomic databases

EPDiO60725.
PaxDbiO60725.
PeptideAtlasiO60725.
PRIDEiO60725.

PTM databases

iPTMnetiO60725.
PhosphoSitePlusiO60725.

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons.1 Publication

Gene expression databases

BgeeiENSG00000116237.
CleanExiHS_ICMT.
ExpressionAtlasiO60725. baseline and differential.
GenevisibleiO60725. HS.

Organism-specific databases

HPAiHPA032024.
HPA032025.

Interactioni

Protein-protein interaction databases

BioGridi117026. 9 interactors.
IntActiO60725. 6 interactors.
STRINGi9606.ENSP00000343552.

Chemistry databases

BindingDBiO60725.

Structurei

3D structure databases

ProteinModelPortaliO60725.
SMRiO60725.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2628. Eukaryota.
COG2020. LUCA.
GeneTreeiENSGT00390000017394.
HOGENOMiHOG000213961.
HOVERGENiHBG019034.
InParanoidiO60725.
KOiK00587.
OMAiNEKAQSH.
OrthoDBiEOG091G0HSB.
PhylomeDBiO60725.
TreeFamiTF313769.

Family and domain databases

InterProiIPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view]
PfamiPF04140. ICMT. 1 hit.
[Graphical view]
PROSITEiPS51564. SAM_ICMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60725-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGCAARAPP GSEARLSLAT FLLGASVLAL PLLTRAGLQG RTGLALYVAG
60 70 80 90 100
LNALLLLLYR PPRYQIAIRA CFLGFVFGCG TLLSFSQSSW SHFGWYMCSL
110 120 130 140 150
SLFHYSEYLV TAVNNPKSLS LDSFLLNHSL EYTVAALSSW LEFTLENIFW
160 170 180 190 200
PELKQITWLS VTGLLMVVFG ECLRKAAMFT AGSNFNHVVQ NEKSDTHTLV
210 220 230 240 250
TSGVYAWFRH PSYVGWFYWS IGTQVMLCNP ICGVSYALTV WRFFRDRTEE
260 270 280
EEISLIHFFG EEYLEYKKRV PTGLPFIKGV KVDL
Length:284
Mass (Da):31,938
Last modified:August 1, 1998 - v1
Checksum:iC86741B13ACA611C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064084 mRNA. Translation: AAC16554.1.
CR541671 mRNA. Translation: CAG46472.1.
CR541711 mRNA. Translation: CAG46512.1.
AL031847 Genomic DNA. Translation: CAI19441.1.
CH471130 Genomic DNA. Translation: EAW71522.1.
BC028168 mRNA. Translation: AAH28168.1.
CCDSiCCDS61.1.
RefSeqiNP_036537.1. NM_012405.3.
UniGeneiHs.515688.

Genome annotation databases

EnsembliENST00000343813; ENSP00000343552; ENSG00000116237.
GeneIDi23463.
KEGGihsa:23463.
UCSCiuc001amk.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064084 mRNA. Translation: AAC16554.1.
CR541671 mRNA. Translation: CAG46472.1.
CR541711 mRNA. Translation: CAG46512.1.
AL031847 Genomic DNA. Translation: CAI19441.1.
CH471130 Genomic DNA. Translation: EAW71522.1.
BC028168 mRNA. Translation: AAH28168.1.
CCDSiCCDS61.1.
RefSeqiNP_036537.1. NM_012405.3.
UniGeneiHs.515688.

3D structure databases

ProteinModelPortaliO60725.
SMRiO60725.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117026. 9 interactors.
IntActiO60725. 6 interactors.
STRINGi9606.ENSP00000343552.

Chemistry databases

BindingDBiO60725.
ChEMBLiCHEMBL4699.

PTM databases

iPTMnetiO60725.
PhosphoSitePlusiO60725.

Polymorphism and mutation databases

BioMutaiICMT.

Proteomic databases

EPDiO60725.
PaxDbiO60725.
PeptideAtlasiO60725.
PRIDEiO60725.

Protocols and materials databases

DNASUi23463.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000343813; ENSP00000343552; ENSG00000116237.
GeneIDi23463.
KEGGihsa:23463.
UCSCiuc001amk.4. human.

Organism-specific databases

CTDi23463.
DisGeNETi23463.
GeneCardsiICMT.
HGNCiHGNC:5350. ICMT.
HPAiHPA032024.
HPA032025.
MIMi605851. gene.
neXtProtiNX_O60725.
OpenTargetsiENSG00000116237.
PharmGKBiPA29598.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2628. Eukaryota.
COG2020. LUCA.
GeneTreeiENSGT00390000017394.
HOGENOMiHOG000213961.
HOVERGENiHBG019034.
InParanoidiO60725.
KOiK00587.
OMAiNEKAQSH.
OrthoDBiEOG091G0HSB.
PhylomeDBiO60725.
TreeFamiTF313769.

Enzyme and pathway databases

BioCyciZFISH:HS03999-MONOMER.
ReactomeiR-HSA-163841. Gamma carboxylation, hypusine formation and arylsulfatase activation.
SABIO-RKO60725.

Miscellaneous databases

GeneWikiiICMT.
GenomeRNAii23463.
PROiO60725.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000116237.
CleanExiHS_ICMT.
ExpressionAtlasiO60725. baseline and differential.
GenevisibleiO60725. HS.

Family and domain databases

InterProiIPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view]
PfamiPF04140. ICMT. 1 hit.
[Graphical view]
PROSITEiPS51564. SAM_ICMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICMT_HUMAN
AccessioniPrimary (citable) accession number: O60725
Secondary accession number(s): Q6FHT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.