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O60725

- ICMT_HUMAN

UniProt

O60725 - ICMT_HUMAN

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Protein

Protein-S-isoprenylcysteine O-methyltransferase

Gene

ICMT

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.

Catalytic activityi

S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Divalent metal cations. Probably Zn(2+).By similarity

GO - Molecular functioni

  1. cAMP response element binding protein binding Source: Ensembl
  2. protein C-terminal carboxyl O-methyltransferase activity Source: ProtInc
  3. protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular protein modification process Source: ProtInc
  2. C-terminal protein methylation Source: ProtInc
  3. in utero embryonic development Source: Ensembl
  4. liver development Source: Ensembl
  5. multicellular organism growth Source: Ensembl
  6. positive regulation of cell proliferation Source: Ensembl
  7. protein targeting to membrane Source: ProtInc
  8. regulation of Ras protein signal transduction Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

SABIO-RKO60725.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100)
Alternative name(s):
Isoprenylcysteine carboxylmethyltransferase
Prenylated protein carboxyl methyltransferase
Short name:
PPMT
Prenylcysteine carboxyl methyltransferase
Short name:
pcCMT
Gene namesi
Name:ICMT
Synonyms:PCCMT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:5350. ICMT.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei17 – 3317HelicalSequence AnalysisAdd
BLAST
Topological domaini34 – 418LumenalSequence Analysis
Transmembranei42 – 5918HelicalSequence AnalysisAdd
BLAST
Topological domaini60 – 6910CytoplasmicSequence Analysis
Transmembranei70 – 8718HelicalSequence AnalysisAdd
BLAST
Topological domaini88 – 925LumenalSequence Analysis
Transmembranei93 – 11220HelicalSequence AnalysisAdd
BLAST
Topological domaini113 – 13119CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei132 – 14918HelicalSequence AnalysisAdd
BLAST
Topological domaini150 – 1545LumenalSequence Analysis
Transmembranei155 – 17420HelicalSequence AnalysisAdd
BLAST
Topological domaini175 – 21238CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei213 – 22816HelicalSequence AnalysisAdd
BLAST
Topological domaini229 – 2291LumenalSequence Analysis
Transmembranei230 – 24415HelicalSequence AnalysisAdd
BLAST
Topological domaini245 – 28440CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29598.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 284284Protein-S-isoprenylcysteine O-methyltransferasePRO_0000209894Add
BLAST

Proteomic databases

MaxQBiO60725.
PaxDbiO60725.
PRIDEiO60725.

PTM databases

PhosphoSiteiO60725.

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons.1 Publication

Gene expression databases

BgeeiO60725.
CleanExiHS_ICMT.
ExpressionAtlasiO60725. baseline and differential.
GenevestigatoriO60725.

Organism-specific databases

HPAiHPA032025.

Interactioni

Protein-protein interaction databases

BioGridi117026. 5 interactions.
STRINGi9606.ENSP00000343552.

Structurei

3D structure databases

ProteinModelPortaliO60725.
SMRiO60725. Positions 153-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2020.
GeneTreeiENSGT00390000017394.
HOGENOMiHOG000213961.
HOVERGENiHBG019034.
InParanoidiO60725.
KOiK00587.
OMAiTTWTHFG.
OrthoDBiEOG7WHHC4.
PhylomeDBiO60725.
TreeFamiTF313769.

Family and domain databases

InterProiIPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view]
PfamiPF04140. ICMT. 1 hit.
[Graphical view]
PROSITEiPS51564. SAM_ICMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O60725-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGCAARAPP GSEARLSLAT FLLGASVLAL PLLTRAGLQG RTGLALYVAG
60 70 80 90 100
LNALLLLLYR PPRYQIAIRA CFLGFVFGCG TLLSFSQSSW SHFGWYMCSL
110 120 130 140 150
SLFHYSEYLV TAVNNPKSLS LDSFLLNHSL EYTVAALSSW LEFTLENIFW
160 170 180 190 200
PELKQITWLS VTGLLMVVFG ECLRKAAMFT AGSNFNHVVQ NEKSDTHTLV
210 220 230 240 250
TSGVYAWFRH PSYVGWFYWS IGTQVMLCNP ICGVSYALTV WRFFRDRTEE
260 270 280
EEISLIHFFG EEYLEYKKRV PTGLPFIKGV KVDL
Length:284
Mass (Da):31,938
Last modified:August 1, 1998 - v1
Checksum:iC86741B13ACA611C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064084 mRNA. Translation: AAC16554.1.
CR541671 mRNA. Translation: CAG46472.1.
CR541711 mRNA. Translation: CAG46512.1.
AL031847 Genomic DNA. Translation: CAI19441.1.
CH471130 Genomic DNA. Translation: EAW71522.1.
BC028168 mRNA. Translation: AAH28168.1.
CCDSiCCDS61.1.
RefSeqiNP_036537.1. NM_012405.3.
XP_005263494.1. XM_005263437.1.
UniGeneiHs.515688.

Genome annotation databases

EnsembliENST00000343813; ENSP00000343552; ENSG00000116237.
GeneIDi23463.
KEGGihsa:23463.
UCSCiuc001amk.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064084 mRNA. Translation: AAC16554.1 .
CR541671 mRNA. Translation: CAG46472.1 .
CR541711 mRNA. Translation: CAG46512.1 .
AL031847 Genomic DNA. Translation: CAI19441.1 .
CH471130 Genomic DNA. Translation: EAW71522.1 .
BC028168 mRNA. Translation: AAH28168.1 .
CCDSi CCDS61.1.
RefSeqi NP_036537.1. NM_012405.3.
XP_005263494.1. XM_005263437.1.
UniGenei Hs.515688.

3D structure databases

ProteinModelPortali O60725.
SMRi O60725. Positions 153-275.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 117026. 5 interactions.
STRINGi 9606.ENSP00000343552.

Chemistry

BindingDBi O60725.
ChEMBLi CHEMBL4699.

PTM databases

PhosphoSitei O60725.

Proteomic databases

MaxQBi O60725.
PaxDbi O60725.
PRIDEi O60725.

Protocols and materials databases

DNASUi 23463.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000343813 ; ENSP00000343552 ; ENSG00000116237 .
GeneIDi 23463.
KEGGi hsa:23463.
UCSCi uc001amk.3. human.

Organism-specific databases

CTDi 23463.
GeneCardsi GC01M006282.
HGNCi HGNC:5350. ICMT.
HPAi HPA032025.
MIMi 605851. gene.
neXtProti NX_O60725.
PharmGKBi PA29598.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG2020.
GeneTreei ENSGT00390000017394.
HOGENOMi HOG000213961.
HOVERGENi HBG019034.
InParanoidi O60725.
KOi K00587.
OMAi TTWTHFG.
OrthoDBi EOG7WHHC4.
PhylomeDBi O60725.
TreeFami TF313769.

Enzyme and pathway databases

SABIO-RK O60725.

Miscellaneous databases

GeneWikii ICMT.
GenomeRNAii 23463.
NextBioi 45777.
PROi O60725.
SOURCEi Search...

Gene expression databases

Bgeei O60725.
CleanExi HS_ICMT.
ExpressionAtlasi O60725. baseline and differential.
Genevestigatori O60725.

Family and domain databases

InterProi IPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view ]
Pfami PF04140. ICMT. 1 hit.
[Graphical view ]
PROSITEi PS51564. SAM_ICMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mammalian prenylcysteine carboxyl methyltransferase is in the endoplasmic reticulum."
    Dai Q., Choy E., Chiu V., Romano J., Slivka S.R., Steitz S.A., Michaelis S., Philips M.R.
    J. Biol. Chem. 273:15030-15034(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Tissue: Myeloid.
  2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "Polyglutamine expansion down-regulates specific neuronal genes before pathologic changes in SCA1."
    Lin X., Antalffy B., Kang D., Orr H.T., Zoghbi H.Y.
    Nat. Neurosci. 3:157-163(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Topology of mammalian isoprenylcysteine carboxyl methyltransferase determined in live cells with a fluorescent probe."
    Wright L.P., Court H., Mor A., Ahearn I.M., Casey P.J., Philips M.R.
    Mol. Cell. Biol. 29:1826-1833(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MEMBRANE TOPOLOGY.

Entry informationi

Entry nameiICMT_HUMAN
AccessioniPrimary (citable) accession number: O60725
Secondary accession number(s): Q6FHT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: August 1, 1998
Last modified: November 26, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3