Reviewed, UniProtKB/Swiss-Prot O60725 (ICMT_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein-S-isoprenylcysteine O-methyltransferase
    EC=2.1.1.100
Alternative name(s):
    Isoprenylcysteine carboxylmethyltransferase
    Prenylcysteine carboxyl methyltransferase
      Short name=pcCMT
    Prenylated protein carboxyl methyltransferase
      Short name=PPMT

Gene names

Name:	ICMT
Synonyms:	PCCMT

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.
Catalytic activity	S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.
Cofactor	Divalent cations. Probably zinc By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue specificity	Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons. Ref.3
Sequence similarities	Belongs to the isoprenylcysteine O-methyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	C-terminal protein amino acid methylation Traceable author statement. Source: ProtInc protein targeting to membrane Ref.1 Traceable author statement. Source: ProtInc
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane fraction Traceable author statement. Source: ProtInc
Molecular function	protein-S-isoprenylcysteine O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Protein-S-isoprenylcysteine O-methyltransferase		PRO_0000209894
Regions
	Transmembrane	18 – 38	21	Potential
	Transmembrane	42 – 62	21	Potential
	Transmembrane	66 – 86	21	Potential
	Transmembrane	214 – 234	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	O60725-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: C86741B13ACA611C	FASTA	284	31,938
	10 20 30 40 50 60 MAGCAARAPP GSEARLSLAT FLLGASVLAL PLLTRAGLQG RTGLALYVAG LNALLLLLYR 70 80 90 100 110 120 PPRYQIAIRA CFLGFVFGCG TLLSFSQSSW SHFGWYMCSL SLFHYSEYLV TAVNNPKSLS 130 140 150 160 170 180 LDSFLLNHSL EYTVAALSSW LEFTLENIFW PELKQITWLS VTGLLMVVFG ECLRKAAMFT 190 200 210 220 230 240 AGSNFNHVVQ NEKSDTHTLV TSGVYAWFRH PSYVGWFYWS IGTQVMLCNP ICGVSYALTV 250 260 270 280 WRFFRDRTEE EEISLIHFFG EEYLEYKKRV PTGLPFIKGV KVDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mammalian prenylcysteine carboxyl methyltransferase is in the endoplasmic reticulum." Dai Q., Choy E., Chiu V., Romano J., Slivka S.R., Steitz S.A., Michaelis S., Philips M.R. J. Biol. Chem. 273:15030-15034(1998) [PubMed: 9614111] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Tissue: Myeloid.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Polyglutamine expansion down-regulates specific neuronal genes before pathologic changes in SCA1." Lin X., Antalffy B., Kang D., Orr H.T., Zoghbi H.Y. Nat. Neurosci. 3:157-163(2000) [PubMed: 10649571] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF064084 mRNA. Translation: AAC16554.1. BC028168 mRNA. Translation: AAH28168.1.
IPI	IPI00031458.
RefSeq	NP_036537.1.
UniGene	Hs.515688 Hs.562083
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	O60725.
Genome annotation databases
Ensembl	ENSG00000116237. Homo sapiens. [Contig view]
GeneID	23463.
KEGG	hsa:23463.
Organism-specific databases
GeneCards	GC01M006215.
H-InvDB	HIX0000076.
HGNC	HGNC:5350. ICMT.
MIM	605851. gene.
PharmGKB	PA29598.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O60725.
HOVERGEN	O60725.
OMA	O60725. ELKQITW.
Enzyme and pathway databases
BRENDA	2.1.1.100. 247.
Gene expression databases
ArrayExpress	O60725.
Bgee	O60725.
CleanEx	HS_ICMT.
GermOnline	ENSG00000116237. Homo sapiens.
Family and domain databases
InterPro	IPR007269. ICMT_MeTrfase. [Graphical view]
Pfam	PF04140. ICMT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	45777.
SOURCE	Search...

Entry information

Entry name

ICMT_HUMAN

Accession

Primary (citable) accession number: O60725

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)