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Protein

UDP-glucose 6-dehydrogenase

Gene

UGDH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.

Catalytic activityi

UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.

Enzyme regulationi

UDP-alpha-D-xylose (UDX) acts as a feedback inhibitor by activating an allosteric switch.1 Publication

Pathwayi: UDP-alpha-D-glucuronate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes UDP-alpha-D-glucuronate from UDP-alpha-D-glucose.
Proteins known to be involved in this subpathway in this organism are:
  1. UDP-glucose 6-dehydrogenase (UGDH)
This subpathway is part of the pathway UDP-alpha-D-glucuronate biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-alpha-D-glucuronate from UDP-alpha-D-glucose, the pathway UDP-alpha-D-glucuronate biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36NAD1 Publication1
Binding sitei41NAD1 Publication1
Binding sitei165NAD1 Publication1
Binding sitei260Substrate1 Publication1
Active sitei276Nucleophile3 Publications1
Binding sitei346NAD1 Publication1
Binding sitei442Substrate1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 16NAD1 Publication6
Nucleotide bindingi89 – 93NAD1 Publication5
Nucleotide bindingi130 – 131NAD1 Publication2
Nucleotide bindingi276 – 279NAD1 Publication4

GO - Molecular functioni

  • electron carrier activity Source: UniProtKB
  • NAD binding Source: InterPro
  • UDP-glucose 6-dehydrogenase activity Source: Reactome

GO - Biological processi

  • carbohydrate metabolic process Source: GO_Central
  • gastrulation with mouth forming second Source: Ensembl
  • glycosaminoglycan biosynthetic process Source: ProtInc
  • UDP-glucose metabolic process Source: ProtInc
  • UDP-glucuronate biosynthetic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciZFISH:HS03260-MONOMER.
BRENDAi1.1.1.22. 2681.
ReactomeiR-HSA-173599. Formation of the active cofactor, UDP-glucuronate.
SABIO-RKO60701.
UniPathwayiUPA00038; UER00491.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucose 6-dehydrogenase (EC:1.1.1.22)
Short name:
UDP-Glc dehydrogenase
Short name:
UDP-GlcDH
Short name:
UDPGDH
Gene namesi
Name:UGDH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:12525. UGDH.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central
  • extracellular exosome Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi7358.
OpenTargetsiENSG00000109814.
PharmGKBiPA37170.

Polymorphism and mutation databases

BioMutaiUGDH.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000740601 – 494UDP-glucose 6-dehydrogenaseAdd BLAST494

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei107N6-acetyllysineCombined sources1
Modified residuei476PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO60701.
MaxQBiO60701.
PaxDbiO60701.
PeptideAtlasiO60701.
PRIDEiO60701.

2D gel databases

REPRODUCTION-2DPAGEO60701.

PTM databases

iPTMnetiO60701.
PhosphoSitePlusiO60701.
SwissPalmiO60701.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000109814.
CleanExiHS_UGDH.
ExpressionAtlasiO60701. baseline and differential.
GenevisibleiO60701. HS.

Organism-specific databases

HPAiCAB034444.
HPA036656.
HPA036657.

Interactioni

Subunit structurei

Homohexamer.3 Publications

Protein-protein interaction databases

BioGridi113205. 38 interactors.
IntActiO60701. 6 interactors.
MINTiMINT-5000810.
STRINGi9606.ENSP00000319501.

Structurei

Secondary structure

1494
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 10Combined sources5
Helixi16 – 26Combined sources11
Beta strandi30 – 35Combined sources6
Helixi39 – 45Combined sources7
Beta strandi47 – 49Combined sources3
Helixi57 – 64Combined sources8
Turni66 – 68Combined sources3
Beta strandi69 – 73Combined sources5
Helixi75 – 81Combined sources7
Beta strandi83 – 87Combined sources5
Beta strandi95 – 97Combined sources3
Turni98 – 102Combined sources5
Helixi107 – 118Combined sources12
Beta strandi122 – 128Combined sources7
Helixi136 – 146Combined sources11
Beta strandi153 – 158Combined sources6
Helixi168 – 173Combined sources6
Beta strandi178 – 181Combined sources4
Helixi186 – 199Combined sources14
Turni200 – 202Combined sources3
Helixi205 – 207Combined sources3
Beta strandi208 – 211Combined sources4
Helixi213 – 244Combined sources32
Helixi248 – 256Combined sources9
Turni259 – 261Combined sources3
Beta strandi263 – 265Combined sources3
Beta strandi274 – 276Combined sources3
Helixi277 – 290Combined sources14
Helixi294 – 321Combined sources28
Turni322 – 324Combined sources3
Beta strandi330 – 334Combined sources5
Beta strandi337 – 339Combined sources3
Helixi349 – 359Combined sources11
Beta strandi363 – 367Combined sources5
Beta strandi369 – 371Combined sources3
Helixi373 – 380Combined sources8
Helixi390 – 394Combined sources5
Beta strandi395 – 397Combined sources3
Helixi401 – 405Combined sources5
Beta strandi409 – 413Combined sources5
Helixi418 – 422Combined sources5
Helixi425 – 431Combined sources7
Beta strandi437 – 442Combined sources6
Turni444 – 447Combined sources4
Helixi449 – 455Combined sources7
Beta strandi458 – 461Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Q3EX-ray2.00A/B/C/D/E/F/G/H/I/J/K/L1-466[»]
2QG4X-ray2.10A/B/C/D/E/F/G/H1-466[»]
3ITKX-ray2.40A/B/C/D/E/F1-466[»]
3KHUX-ray2.30A/B/C/D/E/F1-466[»]
3PRJX-ray3.10A/B/C/D/E/F1-494[»]
3PTZX-ray2.50A/B/C/D/E/F1-494[»]
3TDKX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L1-487[»]
3TF5X-ray2.30A/B/C1-494[»]
4EDFX-ray2.08A/B/C/D1-494[»]
4QEJX-ray2.65A/B/C1-494[»]
4RJTX-ray2.70A/B/C1-494[»]
ProteinModelPortaliO60701.
SMRiO60701.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60701.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni161 – 165Substrate binding1 Publication5
Regioni220 – 224Substrate binding1 Publication5
Regioni267 – 273Substrate binding1 Publication7
Regioni338 – 339Substrate binding1 Publication2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2666. Eukaryota.
COG1004. LUCA.
GeneTreeiENSGT00390000015355.
HOGENOMiHOG000153773.
HOVERGENiHBG003512.
InParanoidiO60701.
KOiK00012.
OMAiVIIMNDY.
OrthoDBiEOG091G05RK.
PhylomeDBiO60701.
TreeFamiTF105671.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
IPR028356. UDPglc_DH_euk.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500133. UDPglc_DH_euk. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O60701-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFEIKKICCI GAGYVGGPTC SVIAHMCPEI RVTVVDVNES RINAWNSPTL
60 70 80 90 100
PIYEPGLKEV VESCRGKNLF FSTNIDDAIK EADLVFISVN TPTKTYGMGK
110 120 130 140 150
GRAADLKYIE ACARRIVQNS NGYKIVTEKS TVPVRAAESI RRIFDANTKP
160 170 180 190 200
NLNLQVLSNP EFLAEGTAIK DLKNPDRVLI GGDETPEGQR AVQALCAVYE
210 220 230 240 250
HWVPREKILT TNTWSSELSK LAANAFLAQR ISSINSISAL CEATGADVEE
260 270 280 290 300
VATAIGMDQR IGNKFLKASV GFGGSCFQKD VLNLVYLCEA LNLPEVARYW
310 320 330 340 350
QQVIDMNDYQ RRRFASRIID SLFNTVTDKK IAILGFAFKK DTGDTRESSS
360 370 380 390 400
IYISKYLMDE GAHLHIYDPK VPREQIVVDL SHPGVSEDDQ VSRLVTISKD
410 420 430 440 450
PYEACDGAHA VVICTEWDMF KELDYERIHK KMLKPAFIFD GRRVLDGLHN
460 470 480 490
ELQTIGFQIE TIGKKVSSKR IPYAPSGEIP KFSLQDPPNK KPKV
Length:494
Mass (Da):55,024
Last modified:August 1, 1998 - v1
Checksum:i9C9DA5E1227D65CC
GO
Isoform 2 (identifier: O60701-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     89-155: Missing.

Note: No experimental confirmation available.
Show »
Length:427
Mass (Da):47,603
Checksum:iDC9E41480CA48E40
GO
Isoform 3 (identifier: O60701-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-97: Missing.

Note: No experimental confirmation available.
Show »
Length:397
Mass (Da):44,415
Checksum:i7CD01E38C0B0D6F5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti338F → V in AAC05135 (Ref. 8) Curated1
Sequence conflicti377V → A in AAC05135 (Ref. 8) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0462341 – 97Missing in isoform 3. 1 PublicationAdd BLAST97
Alternative sequenceiVSP_04255089 – 155Missing in isoform 2. 1 PublicationAdd BLAST67

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061016 mRNA. Translation: AAC36095.1.
AJ007702 mRNA. Translation: CAA07609.1.
AJ272274
, AJ272275, AJ272276, AJ272277, AJ272278, AJ272279, AJ272280, AJ272281 Genomic DNA. Translation: CAB75891.1.
AK097930 mRNA. Translation: BAG53554.1.
AK297737 mRNA. Translation: BAG60087.1.
AC021148 Genomic DNA. No translation available.
CH471069 Genomic DNA. Translation: EAW92937.1.
BC022781 mRNA. Translation: AAH22781.1.
AF049126 mRNA. Translation: AAC05135.1.
CCDSiCCDS3455.1. [O60701-1]
CCDS54757.1. [O60701-3]
CCDS54758.1. [O60701-2]
PIRiJE0353.
RefSeqiNP_001171629.1. NM_001184700.1. [O60701-2]
NP_001171630.1. NM_001184701.1. [O60701-3]
NP_003350.1. NM_003359.3. [O60701-1]
UniGeneiHs.572518.

Genome annotation databases

EnsembliENST00000316423; ENSP00000319501; ENSG00000109814. [O60701-1]
ENST00000501493; ENSP00000422909; ENSG00000109814. [O60701-2]
ENST00000506179; ENSP00000421757; ENSG00000109814. [O60701-1]
ENST00000507089; ENSP00000426560; ENSG00000109814. [O60701-3]
GeneIDi7358.
KEGGihsa:7358.
UCSCiuc003guk.3. human. [O60701-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061016 mRNA. Translation: AAC36095.1.
AJ007702 mRNA. Translation: CAA07609.1.
AJ272274
, AJ272275, AJ272276, AJ272277, AJ272278, AJ272279, AJ272280, AJ272281 Genomic DNA. Translation: CAB75891.1.
AK097930 mRNA. Translation: BAG53554.1.
AK297737 mRNA. Translation: BAG60087.1.
AC021148 Genomic DNA. No translation available.
CH471069 Genomic DNA. Translation: EAW92937.1.
BC022781 mRNA. Translation: AAH22781.1.
AF049126 mRNA. Translation: AAC05135.1.
CCDSiCCDS3455.1. [O60701-1]
CCDS54757.1. [O60701-3]
CCDS54758.1. [O60701-2]
PIRiJE0353.
RefSeqiNP_001171629.1. NM_001184700.1. [O60701-2]
NP_001171630.1. NM_001184701.1. [O60701-3]
NP_003350.1. NM_003359.3. [O60701-1]
UniGeneiHs.572518.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Q3EX-ray2.00A/B/C/D/E/F/G/H/I/J/K/L1-466[»]
2QG4X-ray2.10A/B/C/D/E/F/G/H1-466[»]
3ITKX-ray2.40A/B/C/D/E/F1-466[»]
3KHUX-ray2.30A/B/C/D/E/F1-466[»]
3PRJX-ray3.10A/B/C/D/E/F1-494[»]
3PTZX-ray2.50A/B/C/D/E/F1-494[»]
3TDKX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L1-487[»]
3TF5X-ray2.30A/B/C1-494[»]
4EDFX-ray2.08A/B/C/D1-494[»]
4QEJX-ray2.65A/B/C1-494[»]
4RJTX-ray2.70A/B/C1-494[»]
ProteinModelPortaliO60701.
SMRiO60701.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113205. 38 interactors.
IntActiO60701. 6 interactors.
MINTiMINT-5000810.
STRINGi9606.ENSP00000319501.

PTM databases

iPTMnetiO60701.
PhosphoSitePlusiO60701.
SwissPalmiO60701.

Polymorphism and mutation databases

BioMutaiUGDH.

2D gel databases

REPRODUCTION-2DPAGEO60701.

Proteomic databases

EPDiO60701.
MaxQBiO60701.
PaxDbiO60701.
PeptideAtlasiO60701.
PRIDEiO60701.

Protocols and materials databases

DNASUi7358.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000316423; ENSP00000319501; ENSG00000109814. [O60701-1]
ENST00000501493; ENSP00000422909; ENSG00000109814. [O60701-2]
ENST00000506179; ENSP00000421757; ENSG00000109814. [O60701-1]
ENST00000507089; ENSP00000426560; ENSG00000109814. [O60701-3]
GeneIDi7358.
KEGGihsa:7358.
UCSCiuc003guk.3. human. [O60701-1]

Organism-specific databases

CTDi7358.
DisGeNETi7358.
GeneCardsiUGDH.
HGNCiHGNC:12525. UGDH.
HPAiCAB034444.
HPA036656.
HPA036657.
MIMi603370. gene.
neXtProtiNX_O60701.
OpenTargetsiENSG00000109814.
PharmGKBiPA37170.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2666. Eukaryota.
COG1004. LUCA.
GeneTreeiENSGT00390000015355.
HOGENOMiHOG000153773.
HOVERGENiHBG003512.
InParanoidiO60701.
KOiK00012.
OMAiVIIMNDY.
OrthoDBiEOG091G05RK.
PhylomeDBiO60701.
TreeFamiTF105671.

Enzyme and pathway databases

UniPathwayiUPA00038; UER00491.
BioCyciZFISH:HS03260-MONOMER.
BRENDAi1.1.1.22. 2681.
ReactomeiR-HSA-173599. Formation of the active cofactor, UDP-glucuronate.
SABIO-RKO60701.

Miscellaneous databases

ChiTaRSiUGDH. human.
EvolutionaryTraceiO60701.
GeneWikiiUGDH.
GenomeRNAii7358.
PROiO60701.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000109814.
CleanExiHS_UGDH.
ExpressionAtlasiO60701. baseline and differential.
GenevisibleiO60701. HS.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
IPR028356. UDPglc_DH_euk.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500133. UDPglc_DH_euk. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUGDH_HUMAN
AccessioniPrimary (citable) accession number: O60701
Secondary accession number(s): B3KUU2, B4DN25, O60589
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.