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Protein

Peroxisome biogenesis factor 10

Gene

PEX10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Somewhat implicated in the biogenesis of peroxisomes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri273 – 31139RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • peroxisome organization Source: UniProtKB
  • protein import into peroxisome matrix Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Peroxisome biogenesis

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

TCDBi3.A.20.1.1. the peroxisomal protein importer (ppi) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisome biogenesis factor 10
Alternative name(s):
Peroxin-10
Peroxisomal biogenesis factor 10
Peroxisome assembly protein 10
RING finger protein 69
Gene namesi
Name:PEX10
Synonyms:RNF69
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:8851. PEX10.

Subcellular locationi

GO - Cellular componenti

  • integral component of peroxisomal membrane Source: UniProtKB
  • intracellular Source: GOC
  • peroxisomal membrane Source: UniProtKB
  • peroxisome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Peroxisome

Pathology & Biotechi

Involvement in diseasei

Peroxisome biogenesis disorder complementation group 7 (PBD-CG7)1 Publication

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionA peroxisomal disorder arising from a failure of protein import into the peroxisomal membrane or matrix. The peroxisome biogenesis disorders (PBD group) are genetically heterogeneous with at least 14 distinct genetic groups as concluded from complementation studies. Include disorders are: Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), and classical rhizomelic chondrodysplasia punctata (RCDP). ZWS, NALD and IRD are distinct from RCDP and constitute a clinical continuum of overlapping phenotypes known as the Zellweger spectrum (PBD-ZSS).

See also OMIM:614870
Peroxisome biogenesis disorder 6A (PBD6A)1 Publication

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionA fatal peroxisome biogenesis disorder belonging to the Zellweger disease spectrum and clinically characterized by severe neurologic dysfunction with profound psychomotor retardation, severe hypotonia and neonatal seizures, craniofacial abnormalities, liver dysfunction, and biochemically by the absence of peroxisomes. Additional features include cardiovascular and skeletal defects, renal cysts, ocular abnormalities, and hearing impairment. Most severely affected individuals with the classic form of the disease (classic Zellweger syndrome) die within the first year of life.

See also OMIM:614870
Peroxisome biogenesis disorder 6B (PBD6B)1 Publication

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionA peroxisome biogenesis disorder that includes neonatal adrenoleukodystrophy (NALD) and infantile Refsum disease (IRD), two milder manifestations of the Zellweger disease spectrum. The clinical course of patients with the NALD and IRD presentation is variable and may include developmental delay, hypotonia, liver dysfunction, sensorineural hearing loss, retinal dystrophy and vision impairment. Children with the NALD presentation may reach their teens, while patients with the IRD presentation may reach adulthood. The clinical conditions are often slowly progressive in particular with respect to loss of hearing and vision. The biochemical abnormalities include accumulation of phytanic acid, very long chain fatty acids (VLCFA), di- and trihydroxycholestanoic acid and pipecolic acid.

See also OMIM:614871
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti290 – 2901H → Q in PBD6B; neonatal adrenoleukodystrophy. 1 Publication
VAR_007805

Keywords - Diseasei

Disease mutation, Peroxisome biogenesis disorder, Zellweger syndrome

Organism-specific databases

MIMi614870. phenotype.
614871. phenotype.
Orphaneti247815. Autosomal recessive ataxia due to PEX10 deficiency.
772. Infantile Refsum disease.
44. Neonatal adrenoleukodystrophy.
912. Zellweger syndrome.
PharmGKBiPA33193.

Polymorphism and mutation databases

BioMutaiPEX10.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Peroxisome biogenesis factor 10PRO_0000056376Add
BLAST

Proteomic databases

MaxQBiO60683.
PaxDbiO60683.
PRIDEiO60683.

PTM databases

PhosphoSiteiO60683.

Expressioni

Gene expression databases

BgeeiO60683.
CleanExiHS_PEX10.
ExpressionAtlasiO60683. baseline and differential.
GenevisibleiO60683. HS.

Organism-specific databases

HPAiHPA049458.
HPA049755.

Interactioni

Subunit structurei

Interacts with PEX19.2 Publications

Protein-protein interaction databases

BioGridi111215. 15 interactions.
IntActiO60683. 7 interactions.
STRINGi9606.ENSP00000288774.

Structurei

3D structure databases

ProteinModelPortaliO60683.
SMRiO60683. Positions 272-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pex2/pex10/pex12 family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri273 – 31139RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5574.
GeneTreeiENSGT00510000048446.
HOGENOMiHOG000180194.
HOVERGENiHBG053568.
InParanoidiO60683.
KOiK13346.
OMAiNCTLCLE.
OrthoDBiEOG7C8GHZ.
PhylomeDBiO60683.
TreeFamiTF326491.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR025654. PEX10.
IPR006845. Pex_N.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR23350. PTHR23350. 1 hit.
PfamiPF04757. Pex2_Pex12. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O60683-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPAAASPPE VIRAAQKDEY YRGGLRSAAG GALHSLAGAR KWLEWRKEVE
60 70 80 90 100
LLSDVAYFGL TTLAGYQTLG EEYVSIIQVD PSRIHVPSSL RRGVLVTLHA
110 120 130 140 150
VLPYLLDKAL LPLEQELQAD PDSGRPLQGS LGPGGRGCSG ARRWMRHHTA
160 170 180 190 200
TLTEQQRRAL LRAVFVLRQG LACLQRLHVA WFYIHGVFYH LAKRLTGITY
210 220 230 240 250
LRVRSLPGED LRARVSYRLL GVISLLHLVL SMGLQLYGFR QRQRARKEWR
260 270 280 290 300
LHRGLSHRRA SLEERAVSRN PLCTLCLEER RHPTATPCGH LFCWECITAW
310 320
CSSKAECPLC REKFPPQKLI YLRHYR
Length:326
Mass (Da):37,069
Last modified:August 1, 1998 - v1
Checksum:i9CF2CE5E4C797799
GO
Isoform 2 (identifier: O60683-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     200-200: Y → YQALRPDPLRVLMSVAPSALQ

Note: No experimental confirmation available.
Show »
Length:346
Mass (Da):39,214
Checksum:i0C0720D0E9BF11F8
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti274 – 2741T → A.1 Publication
Corresponds to variant rs34154371 [ dbSNP | Ensembl ].
VAR_058388
Natural varianti290 – 2901H → Q in PBD6B; neonatal adrenoleukodystrophy. 1 Publication
VAR_007805

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei200 – 2001Y → YQALRPDPLRVLMSVAPSAL Q in isoform 2. 1 PublicationVSP_005771

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF060502 mRNA. Translation: AAC18133.1.
AB013818 mRNA. Translation: BAA87895.1.
AK124816 mRNA. Translation: BAG54100.1.
AL513477 Genomic DNA. Translation: CAI22603.1.
CH471183 Genomic DNA. Translation: EAW56105.1.
BC000543 mRNA. Translation: AAH00543.1.
BC018198 mRNA. Translation: AAH18198.1.
CCDSiCCDS41.1. [O60683-2]
CCDS44045.1. [O60683-1]
RefSeqiNP_002608.1. NM_002617.3. [O60683-1]
NP_722540.1. NM_153818.1. [O60683-2]
UniGeneiHs.732228.

Genome annotation databases

EnsembliENST00000288774; ENSP00000288774; ENSG00000157911. [O60683-2]
ENST00000447513; ENSP00000407922; ENSG00000157911. [O60683-1]
GeneIDi5192.
KEGGihsa:5192.
UCSCiuc001ajg.3. human. [O60683-2]
uc001ajh.3. human. [O60683-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

dbPEX, PEX Gene Database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF060502 mRNA. Translation: AAC18133.1.
AB013818 mRNA. Translation: BAA87895.1.
AK124816 mRNA. Translation: BAG54100.1.
AL513477 Genomic DNA. Translation: CAI22603.1.
CH471183 Genomic DNA. Translation: EAW56105.1.
BC000543 mRNA. Translation: AAH00543.1.
BC018198 mRNA. Translation: AAH18198.1.
CCDSiCCDS41.1. [O60683-2]
CCDS44045.1. [O60683-1]
RefSeqiNP_002608.1. NM_002617.3. [O60683-1]
NP_722540.1. NM_153818.1. [O60683-2]
UniGeneiHs.732228.

3D structure databases

ProteinModelPortaliO60683.
SMRiO60683. Positions 272-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111215. 15 interactions.
IntActiO60683. 7 interactions.
STRINGi9606.ENSP00000288774.

Protein family/group databases

TCDBi3.A.20.1.1. the peroxisomal protein importer (ppi) family.

PTM databases

PhosphoSiteiO60683.

Polymorphism and mutation databases

BioMutaiPEX10.

Proteomic databases

MaxQBiO60683.
PaxDbiO60683.
PRIDEiO60683.

Protocols and materials databases

DNASUi5192.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000288774; ENSP00000288774; ENSG00000157911. [O60683-2]
ENST00000447513; ENSP00000407922; ENSG00000157911. [O60683-1]
GeneIDi5192.
KEGGihsa:5192.
UCSCiuc001ajg.3. human. [O60683-2]
uc001ajh.3. human. [O60683-1]

Organism-specific databases

CTDi5192.
GeneCardsiGC01M002368.
GeneReviewsiPEX10.
HGNCiHGNC:8851. PEX10.
HPAiHPA049458.
HPA049755.
MIMi602859. gene.
614870. phenotype.
614871. phenotype.
neXtProtiNX_O60683.
Orphaneti247815. Autosomal recessive ataxia due to PEX10 deficiency.
772. Infantile Refsum disease.
44. Neonatal adrenoleukodystrophy.
912. Zellweger syndrome.
PharmGKBiPA33193.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5574.
GeneTreeiENSGT00510000048446.
HOGENOMiHOG000180194.
HOVERGENiHBG053568.
InParanoidiO60683.
KOiK13346.
OMAiNCTLCLE.
OrthoDBiEOG7C8GHZ.
PhylomeDBiO60683.
TreeFamiTF326491.

Miscellaneous databases

ChiTaRSiPEX10. human.
GeneWikiiPEX10.
GenomeRNAii5192.
NextBioi20080.
PROiO60683.
SOURCEiSearch...

Gene expression databases

BgeeiO60683.
CleanExiHS_PEX10.
ExpressionAtlasiO60683. baseline and differential.
GenevisibleiO60683. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR025654. PEX10.
IPR006845. Pex_N.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR23350. PTHR23350. 1 hit.
PfamiPF04757. Pex2_Pex12. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of PEX10, the gene defective in complementation group 7 of the peroxisome-biogenesis disorders."
    Warren D.S., Morrell J.C., Moser H.W., Valle D., Gould S.J.
    Am. J. Hum. Genet. 63:347-359(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT PBD6B GLN-290.
  2. "Mutations in PEX10 is the cause of Zellweger peroxisome deficiency syndrome of complementation group B."
    Okumoto K., Itoh R., Shimozawa N., Suzuki Y., Tamura S., Kondo N., Fujiki Y.
    Hum. Mol. Genet. 7:1399-1405(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INVOLVEMENT IN PBD6A.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Caudate nucleus.
  4. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Lung.
  7. "PEX19 binds multiple peroxisomal membrane proteins, is predominantly cytoplasmic, and is required for peroxisome membrane synthesis."
    Sacksteder K.A., Jones J.M., South S.T., Li X., Liu Y., Gould S.J.
    J. Cell Biol. 148:931-944(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PEX19.
  8. "Human pex19p binds peroxisomal integral membrane proteins at regions distinct from their sorting sequences."
    Fransen M., Wylin T., Brees C., Mannaerts G.P., Van Veldhoven P.P.
    Mol. Cell. Biol. 21:4413-4424(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PEX19.
  9. "Identification of novel mutations and sequence variation in the Zellweger syndrome spectrum of peroxisome biogenesis disorders."
    Yik W.Y., Steinberg S.J., Moser A.B., Moser H.W., Hacia J.G.
    Hum. Mutat. 30:E467-E480(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT ALA-274, INVOLVEMENT IN PBD-CG7.

Entry informationi

Entry nameiPEX10_HUMAN
AccessioniPrimary (citable) accession number: O60683
Secondary accession number(s): B3KWD8, Q5T095, Q9BW90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: June 24, 2015
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.