Reviewed, UniProtKB/Swiss-Prot O60678 (ANM3_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein arginine N-methyltransferase 3
    EC=2.1.1.-
Alternative name(s):
    Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3

Gene names

Name:	PRMT3
Synonyms:	HRMT1L3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	531 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.
Enzyme regulation	Inhibited by N-ethylmaleimide and high concentrations of zinc chloride By similarity.
Subunit structure	May exist as a monomer or homodimer By similarity.
Subcellular location	Cytoplasm.
Domain	The zinc-finger is responsible for substrate specificity By similarity.
Sequence similarities	Belongs to the protein arginine N-methyltransferase family. Contains 1 C2H2-type zinc finger.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Domain	Zinc-finger
Ligand	Metal-binding S-adenosyl-L-methionine Zinc
Molecular function	Methyltransferase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Ref.2 Non-traceable author statement. Source: UniProtKB
Molecular function	protein-arginine N-methyltransferase activity Ref.2 Non-traceable author statement. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 531

531

Protein arginine N-methyltransferase 3

PRO_0000212326

Regions

Zinc finger

48 – 71

24

C2H2-type

Sites

Binding site

230

1

S-adenosyl-L-methionine By similarity

Binding site

239

1

S-adenosyl-L-methionine

Binding site

263

1

S-adenosyl-L-methionine; via carbonyl oxygen

Binding site

285

1

S-adenosyl-L-methionine

Binding site

314

1

S-adenosyl-L-methionine

Natural variations

Natural variant

440

1

L → V: dbSNP rs3758805.

VAR_024584

Natural variant

470

1

S → C: dbSNP rs11025585.

VAR_030943

Natural variant

508

1

N → S: dbSNP rs6483700.

VAR_024585

Experimental info

Sequence conflict

21

1

L → E in AAC39837. Ref.2

Secondary structure

1

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531

Helix Strand Turn

Details...

Helix

223 – 225

3

Helix

227 – 234

8

Helix

237 – 249

13

Helix

251 – 253

3

Turn

254 – 256

3

Beta strand

258 – 263

6

Helix

268 – 275

8

Beta strand

279 – 287

9

Helix

289 – 299

11

Turn

303 – 305

3

Beta strand

306 – 311

6

Turn

313 – 315

3

Beta strand

323 – 328

6

Turn

336 – 338

3

Helix

340 – 351

12

Beta strand

352 – 360

9

Beta strand

362 – 370

9

Helix

373 – 379

7

Helix

381 – 384

4

Helix

392 – 394

3

Helix

395 – 398

4

Beta strand

403 – 405

3

Helix

409 – 411

3

Beta strand

417 – 423

7

Turn

424 – 426

3

Helix

429 – 432

4

Beta strand

433 – 442

10

Beta strand

446 – 459

14

Beta strand

467 – 470

4

Beta strand

482 – 493

12

Beta strand

498 – 507

10

Beta strand

514 – 521

8

Beta strand

524 – 530

7

Sequences

	Sequence		Length	Mass (Da)
	O60678-1 [UniParc]. Last modified June 7, 2004. Version 2. Checksum: 71EC59E2BEDBE7AC	FASTA	531	59,903
	10 20 30 40 50 60 MCSLASGATG GRGAVENEED LPELSDSGDE AAWEDEDDAD LPHGKQQTPC LFCNRLFTSA 70 80 90 100 110 120 EETFSHCKSE HQFNIDSMVH KHGLEFYGYI KLINFIRLKN PTVEYMNSIY NPVPWEKEEY 130 140 150 160 170 180 LKPVLEDDLL LQFDVEDLYE PVSVPFSYPN GLSENTSVVE KLKHMEARAL SAEAALARAR 190 200 210 220 230 240 EDLQKMKQFA QDFVMHTDVR TCSSSTSVIA DLQEDEDGVY FSSYGHYGIH EEMLKDKIRT 250 260 270 280 290 300 ESYRDFIYQN PHIFKDKVVL DVGCGTGILS MFAAKAGAKK VLGVDQSEIL YQAMDIIRLN 310 320 330 340 350 360 KLEDTITLIK GKIEEVHLPV EKVDVIISEW MGYFLLFESM LDSVLYAKNK YLAKGGSVYP 370 380 390 400 410 420 DICTISLVAV SDVNKHADRI AFWDDVYGFK MSCMKKAVIP EAVVEVLDPK TLISEPCGIK 430 440 450 460 470 480 HIDCHTTSIS DLEFSSDFTL KITRTSMCTA IAGYFDIYFE KNCHNRVVFS TGPQSTKTHW 490 500 510 520 530 KQTVFLLEKP FSVKAGEALK GKVTVHKNKK DPRSLTVTLT LNNSTQTYGL Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"PRMT 3, a type I protein arginine N-methyltransferase that differs from PRMT1 in its oligomerization, subcellular localization, substrate specificity, and regulation." Tang J., Gary J.D., Clarke S., Herschman H.R. J. Biol. Chem. 273:16935-16945(1998) [PubMed: 9642256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-531.
[3]	"The crystal structure of human HMT1 HNRNP methyltransferase-like 3 in complex with SAH." Structural genomics consortium (SGC) Submitted (MAR-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 211-531 IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE.

Cross-references

Sequence databases

EMBLGenBankDDBJ

BC037544 mRNA. Translation: AAH37544.1.
BC064831 mRNA. Translation: AAH64831.1.
AF059531 mRNA. Translation: AAC39837.1.

RefSeq

NP_005779.1.

UniGene

Hs.152337

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FYT	X-ray	2.00	A	211-531	[»]

SMR

O60678. Positions 43-147.

ModBase

Search...

PTM databases

PhosphoSite

O60678.

Genome annotation databases

Ensembl

ENSG00000185238. Homo sapiens. [Contig view]

GeneID

10196.

KEGG

hsa:10196.

Organism-specific databases

H-InvDB

HIX0026130.

HGNC

HGNC:30163. PRMT3.

HPA

HPA007832.

MIM

603190. gene.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOVERGEN

O60678.

Gene expression databases

ArrayExpress

O60678.

CleanEx

HS_PRMT3.

GermOnline

ENSG00000185238. Homo sapiens.

Family and domain databases

InterPro

IPR013216. Methyltransf_11.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]

Pfam

PF08241. Methyltransf_11. 1 hit.
[Graphical view]

SMART

SM00355. ZnF_C2H2. 1 hit.
[Graphical view]

PROSITE

PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. False negative.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

38588.

SOURCE

Search...

Entry information

Entry name

ANM3_HUMAN

Accession

Primary (citable) accession number: O60678

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	June 7, 2004
Last modified:	November 25, 2008
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)