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Protein

Cystatin-8

Gene

CST8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Performs a specialized role during sperm development and maturation.

GO - Molecular functioni

  • cysteine-type endopeptidase inhibitor activity Source: ProtInc

GO - Biological processi

  • negative regulation of endopeptidase activity Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Protein family/group databases

MEROPSiI25.027.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystatin-8
Alternative name(s):
Cystatin-related epididymal spermatogenic protein
Gene namesi
Name:CST8
Synonyms:CRES
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:2480. CST8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26981.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 142121Cystatin-8PRO_0000006653Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi27 – 271N-linked (GlcNAc...)Sequence analysis
Glycosylationi39 – 391N-linked (GlcNAc...)1 Publication
Disulfide bondi95 ↔ 105By similarity
Disulfide bondi119 ↔ 139By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO60676.
PRIDEiO60676.

PTM databases

iPTMnetiO60676.
PhosphoSiteiO60676.

Expressioni

Tissue specificityi

Proximal caput region of the epididymis. Lower expression in the testis. Within the testis it is localized to the elongating spermatids, whereas within the epididymis it is exclusively synthesized by the proximal caput epithelium.

Gene expression databases

BgeeiO60676.
CleanExiHS_CST8.
ExpressionAtlasiO60676. baseline and differential.
GenevisibleiO60676. HS.

Organism-specific databases

HPAiHPA010828.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000246012.

Structurei

3D structure databases

ProteinModelPortaliO60676.
SMRiO60676. Positions 37-142.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi77 – 815Secondary area of contactSequence analysis

Sequence similaritiesi

Belongs to the cystatin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IXH4. Eukaryota.
ENOG41113F6. LUCA.
GeneTreeiENSGT00840000129787.
HOGENOMiHOG000231754.
HOVERGENiHBG095685.
InParanoidiO60676.
KOiK13904.
OMAiKQCLWFA.
PhylomeDBiO60676.

Family and domain databases

InterProiIPR027214. Cystatin.
IPR000010. Cystatin_dom.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 1 hit.
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O60676-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRCRWLSLI LLTIPLALVA RKDPKKNETG VLRKLKPVNA SNANVKQCLW
60 70 80 90 100
FAMQEYNKES EDKYVFLVVK TLQAQLQVTN LLEYLIDVEI ARSDCRKPLS
110 120 130 140
TNEICAIQEN SKLKRKLSCS FLVGALPWNG EFTVMEKKCE DA
Length:142
Mass (Da):16,275
Last modified:August 1, 1998 - v1
Checksum:i9A3512757E0F4ECD
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti52 – 521A → V.
Corresponds to variant rs35190670 [ dbSNP | Ensembl ].
VAR_061130
Natural varianti142 – 1421A → P.
Corresponds to variant rs1054633 [ dbSNP | Ensembl ].
VAR_014527

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059244 mRNA. Translation: AAC14707.1.
AL109954 Genomic DNA. Translation: CAB64234.1.
BC069496 mRNA. Translation: AAH69496.1.
BC105113 mRNA. Translation: AAI05114.1.
BC105119 mRNA. Translation: AAI05120.1.
CCDSiCCDS13156.1.
RefSeqiNP_001268659.1. NM_001281730.1.
NP_005483.1. NM_005492.3.
UniGeneiHs.121602.

Genome annotation databases

EnsembliENST00000246012; ENSP00000246012; ENSG00000125815.
GeneIDi10047.
KEGGihsa:10047.
UCSCiuc002wth.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059244 mRNA. Translation: AAC14707.1.
AL109954 Genomic DNA. Translation: CAB64234.1.
BC069496 mRNA. Translation: AAH69496.1.
BC105113 mRNA. Translation: AAI05114.1.
BC105119 mRNA. Translation: AAI05120.1.
CCDSiCCDS13156.1.
RefSeqiNP_001268659.1. NM_001281730.1.
NP_005483.1. NM_005492.3.
UniGeneiHs.121602.

3D structure databases

ProteinModelPortaliO60676.
SMRiO60676. Positions 37-142.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000246012.

Protein family/group databases

MEROPSiI25.027.

PTM databases

iPTMnetiO60676.
PhosphoSiteiO60676.

Proteomic databases

PaxDbiO60676.
PRIDEiO60676.

Protocols and materials databases

DNASUi10047.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000246012; ENSP00000246012; ENSG00000125815.
GeneIDi10047.
KEGGihsa:10047.
UCSCiuc002wth.3. human.

Organism-specific databases

CTDi10047.
GeneCardsiCST8.
HGNCiHGNC:2480. CST8.
HPAiHPA010828.
MIMi608683. gene.
neXtProtiNX_O60676.
PharmGKBiPA26981.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IXH4. Eukaryota.
ENOG41113F6. LUCA.
GeneTreeiENSGT00840000129787.
HOGENOMiHOG000231754.
HOVERGENiHBG095685.
InParanoidiO60676.
KOiK13904.
OMAiKQCLWFA.
PhylomeDBiO60676.

Miscellaneous databases

GeneWikiiCST8_(gene).
GenomeRNAii10047.
NextBioi37951.
PROiO60676.
SOURCEiSearch...

Gene expression databases

BgeeiO60676.
CleanExiHS_CST8.
ExpressionAtlasiO60676. baseline and differential.
GenevisibleiO60676. HS.

Family and domain databases

InterProiIPR027214. Cystatin.
IPR000010. Cystatin_dom.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 1 hit.
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Transient appearance of CRES protein during spermatogenesis and caput epididymal sperm maturation."
    Cornwall G.A., Hann S.R.
    Mol. Reprod. Dev. 41:37-46(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
    Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A.
    J. Proteome Res. 5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-39.
    Tissue: Saliva.

Entry informationi

Entry nameiCST8_HUMAN
AccessioniPrimary (citable) accession number: O60676
Secondary accession number(s): Q2M2X6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: May 11, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.