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Protein

Cell cycle checkpoint protein RAD1

Gene

RAD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.2 Publications

Catalytic activityi

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.

GO - Molecular functioni

  • 3'-5' exonuclease activity Source: UniProtKB
  • damaged DNA binding Source: ProtInc
  • exodeoxyribonuclease III activity Source: UniProtKB-EC

GO - Biological processi

  • cellular response to DNA damage stimulus Source: ProtInc
  • cellular response to ionizing radiation Source: UniProtKB
  • DNA damage checkpoint Source: UniProtKB
  • DNA repair Source: ProtInc
  • DNA replication Source: Reactome
  • meiotic recombination checkpoint Source: UniProtKB
  • regulation of signal transduction by p53 class mediator Source: Reactome
  • substantia nigra development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciZFISH:HS03684-MONOMER.
ReactomeiR-HSA-176187. Activation of ATR in response to replication stress.
R-HSA-5685938. HDR through Single Strand Annealing (SSA).
R-HSA-5693607. Processing of DNA double-strand break ends.
R-HSA-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-69473. G2/M DNA damage checkpoint.
SIGNORiO60671.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell cycle checkpoint protein RAD1 (EC:3.1.11.2)
Short name:
hRAD1
Alternative name(s):
DNA repair exonuclease rad1 homolog
Rad1-like DNA damage checkpoint protein
Gene namesi
Name:RAD1
Synonyms:REC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:9806. RAD1.

Subcellular locationi

GO - Cellular componenti

  • checkpoint clamp complex Source: GO_Central
  • chromosome Source: UniProtKB
  • intracellular membrane-bounded organelle Source: HPA
  • nucleoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi226 – 233SLLKPSTK → AAAAAAAA: Abolishes association of the 9-1-1 complex with RAD17. 1 Publication8

Organism-specific databases

DisGeNETi5810.
OpenTargetsiENSG00000113456.
PharmGKBiPA34166.

Chemistry databases

ChEMBLiCHEMBL3309116.

Polymorphism and mutation databases

BioMutaiRAD1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002250051 – 282Cell cycle checkpoint protein RAD1Add BLAST282

Proteomic databases

EPDiO60671.
MaxQBiO60671.
PaxDbiO60671.
PeptideAtlasiO60671.
PRIDEiO60671.

PTM databases

iPTMnetiO60671.
PhosphoSitePlusiO60671.

Expressioni

Tissue specificityi

Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000113456.
CleanExiHS_RAD1.
ExpressionAtlasiO60671. baseline and differential.
GenevisibleiO60671. HS.

Organism-specific databases

HPAiHPA006692.

Interactioni

Subunit structurei

Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD1 interacts with POLB, FEN1, HUS1, HUS1B, RAD9A and RAD9B. Interacts with DNAJC7.13 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HUS1O609214EBI-721835,EBI-1056174
RAD9AQ996382EBI-721835,EBI-2606224

Protein-protein interaction databases

BioGridi111771. 19 interactors.
DIPiDIP-46061N.
IntActiO60671. 4 interactors.
MINTiMINT-1853000.
STRINGi9606.ENSP00000340879.

Chemistry databases

BindingDBiO60671.

Structurei

Secondary structure

1282
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi16 – 22Combined sources7
Helixi25 – 32Combined sources8
Beta strandi36 – 45Combined sources10
Beta strandi48 – 55Combined sources8
Turni56 – 58Combined sources3
Beta strandi59 – 66Combined sources8
Helixi67 – 69Combined sources3
Beta strandi70 – 78Combined sources9
Beta strandi80 – 85Combined sources6
Helixi86 – 93Combined sources8
Turni94 – 96Combined sources3
Beta strandi107 – 112Combined sources6
Beta strandi114 – 117Combined sources4
Beta strandi119 – 125Combined sources7
Beta strandi128 – 134Combined sources7
Turni149 – 151Combined sources3
Beta strandi152 – 159Combined sources8
Helixi160 – 163Combined sources4
Helixi164 – 168Combined sources5
Beta strandi174 – 181Combined sources8
Beta strandi183 – 194Combined sources12
Beta strandi197 – 203Combined sources7
Beta strandi205 – 207Combined sources3
Beta strandi208 – 215Combined sources8
Beta strandi219 – 224Combined sources6
Helixi225 – 228Combined sources4
Helixi231 – 237Combined sources7
Beta strandi239 – 246Combined sources8
Beta strandi247 – 249Combined sources3
Beta strandi251 – 258Combined sources8
Beta strandi260 – 262Combined sources3
Beta strandi264 – 271Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A1JX-ray2.50C13-275[»]
3G65X-ray2.90B1-282[»]
3GGRX-ray3.20C1-282[»]
ProteinModelPortaliO60671.
SMRiO60671.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60671.

Family & Domainsi

Sequence similaritiesi

Belongs to the rad1 family.Curated

Phylogenomic databases

eggNOGiKOG3194. Eukaryota.
ENOG410YHQU. LUCA.
GeneTreeiENSGT00500000044913.
HOGENOMiHOG000008015.
HOVERGENiHBG053058.
InParanoidiO60671.
KOiK02830.
OMAiCKVSVRT.
OrthoDBiEOG091G0HS8.
PhylomeDBiO60671.
TreeFamiTF101211.

Family and domain databases

InterProiIPR003011. Cell_cycle_checkpoint_Rad1.
IPR003021. Rad1_Rec1_Rad17.
[Graphical view]
PANTHERiPTHR10870. PTHR10870. 1 hit.
PfamiPF02144. Rad1. 1 hit.
[Graphical view]
PRINTSiPR01245. RAD1REC1.
PR01246. RAD1REPAIR.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O60671-1) [UniParc]FASTAAdd to basket
Also known as: Hrad1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK
60 70 80 90 100
VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP
110 120 130 140 150
MPGTLTALRM CYQGYGYPLM LFLEEGGVVT VCKINTQEPE ETLDFDFCST
160 170 180 190 200
NVINKIILQS EGLREAFSEL DMTSEVLQIT MSPDKPYFRL STFGNAGSSH
210 220 230 240 250
LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK VSIRTDNRGF
260 270 280
LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES
Length:282
Mass (Da):31,827
Last modified:August 1, 1998 - v1
Checksum:i075FBD4CF8A4FDB2
GO
Isoform 2 (identifier: O60671-2) [UniParc]FASTAAdd to basket
Also known as: Hrad1B

The sequence of this isoform differs from the canonical sequence as follows:
     67-102: Missing.

Show »
Length:246
Mass (Da):27,828
Checksum:iE96C130821B8D2D0
GO
Isoform 3 (identifier: O60671-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     67-68: AG → GL
     69-282: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:68
Mass (Da):7,576
Checksum:i12B4E5E1CA5B9703
GO

Sequence cautioni

The sequence AAC35550 differs from that shown. Reason: Frameshift at position 103.Curated
The sequence AAC35550 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA06249 differs from that shown. Reason: Frameshift at position 103.Curated
The sequence CAA06249 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti135N → T in AAC95466 (PubMed:9828137).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05171833A → G.Corresponds to variant rs2308951dbSNPEnsembl.1
Natural variantiVAR_05537639H → Q.1 PublicationCorresponds to variant rs41271673dbSNPEnsembl.1
Natural variantiVAR_051719104T → S.Corresponds to variant rs1805328dbSNPEnsembl.1
Natural variantiVAR_051720114G → D.Corresponds to variant rs2308957dbSNPEnsembl.1
Natural variantiVAR_051721281E → G.1 PublicationCorresponds to variant rs1805327dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01733467 – 102Missing in isoform 2. 2 PublicationsAdd BLAST36
Alternative sequenceiVSP_01733567 – 68AG → GL in isoform 3. 1 Publication2
Alternative sequenceiVSP_01733669 – 282Missing in isoform 3. 1 PublicationAdd BLAST214

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074717 mRNA. Translation: AAC98093.1.
AF073524 mRNA. Translation: AAC95466.1.
AF030933 mRNA. Translation: AAC95427.1.
AF076841 mRNA. Translation: AAC95523.1.
AF090170 mRNA. Translation: AAC95603.1.
AJ004974 mRNA. Translation: CAA06248.1.
AJ004975 mRNA. Translation: CAA06249.1. Sequence problems.
AF011905 mRNA. Translation: AAC27243.1.
AF058392 mRNA. Translation: AAC14138.1.
AF084512 mRNA. Translation: AAC35549.1.
AF084513 mRNA. Translation: AAC35550.1. Sequence problems.
AK002112 mRNA. Translation: BAG51017.1.
BT006908 mRNA. Translation: AAP35554.1.
DQ451401 Genomic DNA. Translation: ABD96829.1.
CH471119 Genomic DNA. Translation: EAW55904.1.
BC006837 mRNA. Translation: AAH06837.1.
BC009804 mRNA. Translation: AAH09804.1.
BC037857 mRNA. Translation: AAH37857.1.
AB183821 Genomic DNA. Translation: BAD86789.1.
AB183822 Genomic DNA. Translation: BAD86790.1.
CCDSiCCDS3905.1. [O60671-1]
RefSeqiNP_002844.1. NM_002853.3. [O60671-1]
UniGeneiHs.38114.

Genome annotation databases

EnsembliENST00000325577; ENSP00000313467; ENSG00000113456. [O60671-3]
ENST00000341754; ENSP00000340879; ENSG00000113456. [O60671-1]
ENST00000382038; ENSP00000371469; ENSG00000113456. [O60671-1]
GeneIDi5810.
KEGGihsa:5810.
UCSCiuc003jix.4. human. [O60671-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074717 mRNA. Translation: AAC98093.1.
AF073524 mRNA. Translation: AAC95466.1.
AF030933 mRNA. Translation: AAC95427.1.
AF076841 mRNA. Translation: AAC95523.1.
AF090170 mRNA. Translation: AAC95603.1.
AJ004974 mRNA. Translation: CAA06248.1.
AJ004975 mRNA. Translation: CAA06249.1. Sequence problems.
AF011905 mRNA. Translation: AAC27243.1.
AF058392 mRNA. Translation: AAC14138.1.
AF084512 mRNA. Translation: AAC35549.1.
AF084513 mRNA. Translation: AAC35550.1. Sequence problems.
AK002112 mRNA. Translation: BAG51017.1.
BT006908 mRNA. Translation: AAP35554.1.
DQ451401 Genomic DNA. Translation: ABD96829.1.
CH471119 Genomic DNA. Translation: EAW55904.1.
BC006837 mRNA. Translation: AAH06837.1.
BC009804 mRNA. Translation: AAH09804.1.
BC037857 mRNA. Translation: AAH37857.1.
AB183821 Genomic DNA. Translation: BAD86789.1.
AB183822 Genomic DNA. Translation: BAD86790.1.
CCDSiCCDS3905.1. [O60671-1]
RefSeqiNP_002844.1. NM_002853.3. [O60671-1]
UniGeneiHs.38114.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A1JX-ray2.50C13-275[»]
3G65X-ray2.90B1-282[»]
3GGRX-ray3.20C1-282[»]
ProteinModelPortaliO60671.
SMRiO60671.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111771. 19 interactors.
DIPiDIP-46061N.
IntActiO60671. 4 interactors.
MINTiMINT-1853000.
STRINGi9606.ENSP00000340879.

Chemistry databases

BindingDBiO60671.
ChEMBLiCHEMBL3309116.

PTM databases

iPTMnetiO60671.
PhosphoSitePlusiO60671.

Polymorphism and mutation databases

BioMutaiRAD1.

Proteomic databases

EPDiO60671.
MaxQBiO60671.
PaxDbiO60671.
PeptideAtlasiO60671.
PRIDEiO60671.

Protocols and materials databases

DNASUi5810.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000325577; ENSP00000313467; ENSG00000113456. [O60671-3]
ENST00000341754; ENSP00000340879; ENSG00000113456. [O60671-1]
ENST00000382038; ENSP00000371469; ENSG00000113456. [O60671-1]
GeneIDi5810.
KEGGihsa:5810.
UCSCiuc003jix.4. human. [O60671-1]

Organism-specific databases

CTDi5810.
DisGeNETi5810.
GeneCardsiRAD1.
HGNCiHGNC:9806. RAD1.
HPAiHPA006692.
MIMi603153. gene.
neXtProtiNX_O60671.
OpenTargetsiENSG00000113456.
PharmGKBiPA34166.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3194. Eukaryota.
ENOG410YHQU. LUCA.
GeneTreeiENSGT00500000044913.
HOGENOMiHOG000008015.
HOVERGENiHBG053058.
InParanoidiO60671.
KOiK02830.
OMAiCKVSVRT.
OrthoDBiEOG091G0HS8.
PhylomeDBiO60671.
TreeFamiTF101211.

Enzyme and pathway databases

BioCyciZFISH:HS03684-MONOMER.
ReactomeiR-HSA-176187. Activation of ATR in response to replication stress.
R-HSA-5685938. HDR through Single Strand Annealing (SSA).
R-HSA-5693607. Processing of DNA double-strand break ends.
R-HSA-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-69473. G2/M DNA damage checkpoint.
SIGNORiO60671.

Miscellaneous databases

ChiTaRSiRAD1. human.
EvolutionaryTraceiO60671.
GeneWikiiRAD1_homolog.
GenomeRNAii5810.
PROiO60671.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000113456.
CleanExiHS_RAD1.
ExpressionAtlasiO60671. baseline and differential.
GenevisibleiO60671. HS.

Family and domain databases

InterProiIPR003011. Cell_cycle_checkpoint_Rad1.
IPR003021. Rad1_Rec1_Rad17.
[Graphical view]
PANTHERiPTHR10870. PTHR10870. 1 hit.
PfamiPF02144. Rad1. 1 hit.
[Graphical view]
PRINTSiPR01245. RAD1REC1.
PR01246. RAD1REPAIR.
ProtoNetiSearch...

Entry informationi

Entry nameiRAD1_HUMAN
AccessioniPrimary (citable) accession number: O60671
Secondary accession number(s): O75572
, O95304, Q1W161, Q5KSM0, Q5KSM1, Q9UEP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.