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O60669

- MOT2_HUMAN

UniProt

O60669 - MOT2_HUMAN

Protein

Monocarboxylate transporter 2

Gene

SLC16A7

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (30 Nov 2010)
      Previous versions | rss
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    Functioni

    Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Functions as high-affinity pyruvate transporter.1 Publication

    GO - Molecular functioni

    1. lactate transmembrane transporter activity Source: UniProtKB
    2. pyruvate secondary active transmembrane transporter activity Source: ProtInc
    3. pyruvate transmembrane transporter activity Source: UniProtKB
    4. secondary active monocarboxylate transmembrane transporter activity Source: InterPro
    5. symporter activity Source: UniProtKB-KW

    GO - Biological processi

    1. lactate transmembrane transport Source: UniProtKB
    2. pyruvate transmembrane transport Source: UniProtKB
    3. transmembrane transport Source: Reactome

    Keywords - Biological processi

    Symport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_20515. Proton-coupled monocarboxylate transport.
    SABIO-RKO60669.

    Protein family/group databases

    TCDBi2.A.1.13.5. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Monocarboxylate transporter 2
    Short name:
    MCT 2
    Alternative name(s):
    Solute carrier family 16 member 7
    Gene namesi
    Name:SLC16A7
    Synonyms:MCT2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:10928. SLC16A7.

    Subcellular locationi

    Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. integral component of plasma membrane Source: UniProtKB
    2. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35819.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 478478Monocarboxylate transporter 2PRO_0000211385Add
    BLAST

    Proteomic databases

    MaxQBiO60669.
    PaxDbiO60669.
    PRIDEiO60669.

    PTM databases

    PhosphoSiteiO60669.

    Expressioni

    Tissue specificityi

    Detected in heart and in blood lymphocytes and monocytes (at protein level). High expression in testis, moderate to low in spleen, heart, kidney, pancreas, skeletal muscle, brain and Leukocyte. Restricted expression in normal tissues, but widely expressed in cancer cells.1 Publication

    Gene expression databases

    ArrayExpressiO60669.
    BgeeiO60669.
    CleanExiHS_SLC16A7.
    GenevestigatoriO60669.

    Organism-specific databases

    HPAiHPA005911.

    Interactioni

    Subunit structurei

    Interacts with GRID2IP.By similarity

    Protein-protein interaction databases

    BioGridi114629. 3 interactions.
    IntActiO60669. 1 interaction.
    STRINGi9606.ENSP00000261187.

    Structurei

    3D structure databases

    ProteinModelPortaliO60669.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1515CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini37 – 5923ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini81 – 899CytoplasmicSequence Analysis
    Topological domaini111 – 1155ExtracellularSequence Analysis
    Topological domaini137 – 14812CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini170 – 1734ExtracellularSequence Analysis
    Topological domaini195 – 24652CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini268 – 28215ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini304 – 3129CytoplasmicSequence Analysis
    Topological domaini334 – 3385ExtracellularSequence Analysis
    Topological domaini360 – 37314CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini395 – 40612ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini428 – 47851CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei60 – 8021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei90 – 11021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei116 – 13621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei149 – 16921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei174 – 19421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei247 – 26721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei283 – 30321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei313 – 33321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei339 – 35921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei374 – 39421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei407 – 42721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    HOGENOMiHOG000280688.
    HOVERGENiHBG006384.
    InParanoidiO60669.
    KOiK08184.
    OMAiCCLGMVT.
    OrthoDBiEOG7W9RTN.
    PhylomeDBiO60669.
    TreeFamiTF313792.

    Family and domain databases

    InterProiIPR027178. MCT2.
    IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004743. Monocarb_transpt.
    [Graphical view]
    PANTHERiPTHR11360:SF25. PTHR11360:SF25. 1 hit.
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00892. 2A0113. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O60669-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPMPSAPPV HPPPDGGWGW IVVGAAFISI GFSYAFPKAV TVFFKEIQQI    50
    FHTTYSEIAW ISSIMLAVMY AGGPVSSVLV NKYGSRPVVI AGGLLCCLGM 100
    VLASFSSSVV QLYLTMGFIT GLGLAFNLQP ALTIIGKYFY RKRPMANGLA 150
    MAGSPVFLSS LAPFNQYLFN TFGWKGSFLI LGSLLLNACV AGSLMRPLGP 200
    NQTTSKSKNK TGKTEDDSSP KKIKTKKSTW EKVNKYLDFS LFKHRGFLIY 250
    LSGNVIMFLG FFAPIIFLAP YAKDQGIDEY SAAFLLSVMA FVDMFARPSV 300
    GLIANSKYIR PRIQYFFSFA IMFNGVCHLL CPLAQDYTSL VLYAVFFGLG 350
    FGSVSSVLFE TLMDLVGAPR FSSAVGLVTI VECGPVLLGP PLAGKLVDLT 400
    GEYKYMYMSC GAIVVAASVW LLIGNAINYR LLAKERKEEN ARQKTRESEP 450
    LSKSKHSEDV NVKVSNAQSV TSERETNI 478
    Length:478
    Mass (Da):52,200
    Last modified:November 30, 2010 - v2
    Checksum:iE508D650E8D7DE98
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261A → T in AAC70919. 1 PublicationCurated
    Sequence conflicti154 – 1541S → N in AAC70919. 1 PublicationCurated
    Sequence conflicti268 – 2681L → P in AAC70919. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti445 – 4451T → S.1 Publication
    Corresponds to variant rs3763980 [ dbSNP | Ensembl ].
    VAR_031660

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF049608 mRNA. Translation: AAC70919.1.
    AF058056 mRNA. Translation: AAC13721.1.
    AK313345 mRNA. Translation: BAG36148.1.
    AC079905 Genomic DNA. No translation available.
    CH471054 Genomic DNA. Translation: EAW97096.1.
    BC030693 mRNA. Translation: AAH30693.1.
    CCDSiCCDS8961.1.
    RefSeqiNP_001257551.1. NM_001270622.1.
    NP_001257552.1. NM_001270623.1.
    NP_004722.2. NM_004731.4.
    XP_005269288.1. XM_005269231.2.
    XP_006719756.1. XM_006719693.1.
    UniGeneiHs.439643.
    Hs.88156.

    Genome annotation databases

    EnsembliENST00000261187; ENSP00000261187; ENSG00000118596.
    ENST00000547379; ENSP00000448071; ENSG00000118596.
    ENST00000552024; ENSP00000448742; ENSG00000118596.
    ENST00000552432; ENSP00000449547; ENSG00000118596.
    GeneIDi9194.
    KEGGihsa:9194.
    UCSCiuc001sqs.4. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF049608 mRNA. Translation: AAC70919.1 .
    AF058056 mRNA. Translation: AAC13721.1 .
    AK313345 mRNA. Translation: BAG36148.1 .
    AC079905 Genomic DNA. No translation available.
    CH471054 Genomic DNA. Translation: EAW97096.1 .
    BC030693 mRNA. Translation: AAH30693.1 .
    CCDSi CCDS8961.1.
    RefSeqi NP_001257551.1. NM_001270622.1.
    NP_001257552.1. NM_001270623.1.
    NP_004722.2. NM_004731.4.
    XP_005269288.1. XM_005269231.2.
    XP_006719756.1. XM_006719693.1.
    UniGenei Hs.439643.
    Hs.88156.

    3D structure databases

    ProteinModelPortali O60669.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114629. 3 interactions.
    IntActi O60669. 1 interaction.
    STRINGi 9606.ENSP00000261187.

    Chemistry

    ChEMBLi CHEMBL2073704.
    DrugBanki DB00119. Pyruvic acid.

    Protein family/group databases

    TCDBi 2.A.1.13.5. the major facilitator superfamily (mfs).

    PTM databases

    PhosphoSitei O60669.

    Proteomic databases

    MaxQBi O60669.
    PaxDbi O60669.
    PRIDEi O60669.

    Protocols and materials databases

    DNASUi 9194.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000261187 ; ENSP00000261187 ; ENSG00000118596 .
    ENST00000547379 ; ENSP00000448071 ; ENSG00000118596 .
    ENST00000552024 ; ENSP00000448742 ; ENSG00000118596 .
    ENST00000552432 ; ENSP00000449547 ; ENSG00000118596 .
    GeneIDi 9194.
    KEGGi hsa:9194.
    UCSCi uc001sqs.4. human.

    Organism-specific databases

    CTDi 9194.
    GeneCardsi GC12P059989.
    H-InvDB HIX0010769.
    HGNCi HGNC:10928. SLC16A7.
    HPAi HPA005911.
    MIMi 603654. gene.
    neXtProti NX_O60669.
    PharmGKBi PA35819.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0477.
    HOGENOMi HOG000280688.
    HOVERGENi HBG006384.
    InParanoidi O60669.
    KOi K08184.
    OMAi CCLGMVT.
    OrthoDBi EOG7W9RTN.
    PhylomeDBi O60669.
    TreeFami TF313792.

    Enzyme and pathway databases

    Reactomei REACT_20515. Proton-coupled monocarboxylate transport.
    SABIO-RK O60669.

    Miscellaneous databases

    GenomeRNAii 9194.
    NextBioi 34471.
    PROi O60669.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O60669.
    Bgeei O60669.
    CleanExi HS_SLC16A7.
    Genevestigatori O60669.

    Family and domain databases

    InterProi IPR027178. MCT2.
    IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004743. Monocarb_transpt.
    [Graphical view ]
    PANTHERi PTHR11360:SF25. PTHR11360:SF25. 1 hit.
    Pfami PF07690. MFS_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 1 hit.
    TIGRFAMsi TIGR00892. 2A0113. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human monocarboxylate transporter 2 (MCT2) is a high affinity pyruvate transporter."
      Lin R.-Y., Vera J.C., Chaganti R.S.K., Golde D.W.
      J. Biol. Chem. 273:28959-28965(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
      Tissue: Liver.
    2. "Cloning of human monocarboxylate transporter 2 (hMCT2)."
      Dao L., Landschulz W.H., Landschulz K.T.
      Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-445.
      Tissue: Liver.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    4. "The finished DNA sequence of human chromosome 12."
      Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
      , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
      Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    7. "Presence and localization of three lactic acid transporters (MCT1, -2, and -4) in separated human granulocytes, lymphocytes, and monocytes."
      Merezhinskaya N., Ogunwuyi S.A., Mullick F.G., Fishbein W.N.
      J. Histochem. Cytochem. 52:1483-1493(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiMOT2_HUMAN
    AccessioniPrimary (citable) accession number: O60669
    Secondary accession number(s): Q8NEM3, Q9UPB3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 30, 2010
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3