O60669 (MOT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Monocarboxylate transporter 2
Short name=MCT 2

Alternative name(s):
Solute carrier family 16 member 7

Gene names

Name:	SLC16A7
Synonyms:	MCT2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. MCT2 is a high affinity pyruvate transporter.
Subunit structure	Interacts with GRID2IP By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	High expression in testis, moderate to low in spleen, heart, kidney, pancreas, skeletal muscle, brain and Leukocyte. Restricted expression in normal tissues, but widely expressed in cancer cells.
Sequence similarities	Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. [View classification]

Ontologies

Keywords
Biological process	Symport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	pyruvate secondary active transmembrane transporter activity Traceable author statement Ref.1. Source: ProtInc secondary active monocarboxylate transmembrane transporter activity Inferred from electronic annotation. Source: InterPro symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Monocarboxylate transporter 2		PRO_0000211385
Regions
	Topological domain	1 – 15	15	Cytoplasmic Potential
	Transmembrane	16 – 36	21	Helical; Potential
	Topological domain	37 – 59	23	Extracellular Potential
	Transmembrane	60 – 80	21	Helical; Potential
	Topological domain	81 – 89	9	Cytoplasmic Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Topological domain	111 – 115	5	Extracellular Potential
	Transmembrane	116 – 136	21	Helical; Potential
	Topological domain	137 – 148	12	Cytoplasmic Potential
	Transmembrane	149 – 169	21	Helical; Potential
	Topological domain	170 – 173	4	Extracellular Potential
	Transmembrane	174 – 194	21	Helical; Potential
	Topological domain	195 – 246	52	Cytoplasmic Potential
	Transmembrane	247 – 267	21	Helical; Potential
	Topological domain	268 – 282	15	Extracellular Potential
	Transmembrane	283 – 303	21	Helical; Potential
	Topological domain	304 – 312	9	Cytoplasmic Potential
	Transmembrane	313 – 333	21	Helical; Potential
	Topological domain	334 – 338	5	Extracellular Potential
	Transmembrane	339 – 359	21	Helical; Potential
	Topological domain	360 – 373	14	Cytoplasmic Potential
	Transmembrane	374 – 394	21	Helical; Potential
	Topological domain	395 – 406	12	Extracellular Potential
	Transmembrane	407 – 427	21	Helical; Potential
	Topological domain	428 – 478	51	Cytoplasmic Potential
Natural variations
	Natural variant	445	1	T → S. Ref.2 Corresponds to variant rs3763980 [ dbSNP | Ensembl ].		VAR_031660
Experimental info
	Sequence conflict	26	1	A → T in AAC70919. Ref.2
	Sequence conflict	154	1	S → N in AAC70919. Ref.2
	Sequence conflict	268	1	L → P in AAC70919. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O60669 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: E508D650E8D7DE98	FASTA	478	52,200
	10 20 30 40 50 60 MPPMPSAPPV HPPPDGGWGW IVVGAAFISI GFSYAFPKAV TVFFKEIQQI FHTTYSEIAW 70 80 90 100 110 120 ISSIMLAVMY AGGPVSSVLV NKYGSRPVVI AGGLLCCLGM VLASFSSSVV QLYLTMGFIT 130 140 150 160 170 180 GLGLAFNLQP ALTIIGKYFY RKRPMANGLA MAGSPVFLSS LAPFNQYLFN TFGWKGSFLI 190 200 210 220 230 240 LGSLLLNACV AGSLMRPLGP NQTTSKSKNK TGKTEDDSSP KKIKTKKSTW EKVNKYLDFS 250 260 270 280 290 300 LFKHRGFLIY LSGNVIMFLG FFAPIIFLAP YAKDQGIDEY SAAFLLSVMA FVDMFARPSV 310 320 330 340 350 360 GLIANSKYIR PRIQYFFSFA IMFNGVCHLL CPLAQDYTSL VLYAVFFGLG FGSVSSVLFE 370 380 390 400 410 420 TLMDLVGAPR FSSAVGLVTI VECGPVLLGP PLAGKLVDLT GEYKYMYMSC GAIVVAASVW 430 440 450 460 470 LLIGNAINYR LLAKERKEEN ARQKTRESEP LSKSKHSEDV NVKVSNAQSV TSERETNI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human monocarboxylate transporter 2 (MCT2) is a high affinity pyruvate transporter." Lin R.-Y., Vera J.C., Chaganti R.S.K., Golde D.W. J. Biol. Chem. 273:28959-28965(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Cloning of human monocarboxylate transporter 2 (hMCT2)." Dao L., Landschulz W.H., Landschulz K.T. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-445. Tissue: Liver.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF049608 mRNA. Translation: AAC70919.1. AF058056 mRNA. Translation: AAC13721.1. AK313345 mRNA. Translation: BAG36148.1. AC079905 Genomic DNA. No translation available. CH471054 Genomic DNA. Translation: EAW97096.1. BC030693 mRNA. Translation: AAH30693.1.
IPI	IPI00171614.
RefSeq	NP_001257551.1. NM_001270622.1. NP_001257552.1. NM_001270623.1. NP_004722.2. NM_004731.4.
UniGene	Hs.439643. Hs.88156.
3D structure databases
ProteinModelPortal	O60669.
ModBase	Search...
Protein-protein interaction databases
IntAct	O60669. 1 interaction.
STRING	9606.ENSP00000261187.
Protein family/group databases
TCDB	2.A.1.13.5. major facilitator superfamily (MFS).
PTM databases
PhosphoSite	O60669.
Proteomic databases
PaxDb	O60669.
PRIDE	O60669.
Protocols and materials databases
DNASU	9194.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000261187; ENSP00000261187; ENSG00000118596. ENST00000547379; ENSP00000448071; ENSG00000118596. ENST00000552024; ENSP00000448742; ENSG00000118596. ENST00000552432; ENSP00000449547; ENSG00000118596.
GeneID	9194.
KEGG	hsa:9194.
UCSC	uc001sqs.3. human.
Organism-specific databases
CTD	9194.
GeneCards	GC12P059989.
H-InvDB	HIX0010769.
HGNC	HGNC:10928. SLC16A7.
HPA	HPA005911.
MIM	603654. gene.
neXtProt	NX_O60669.
PharmGKB	PA35819.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0477.
HOGENOM	HOG000280688.
HOVERGEN	HBG006384.
InParanoid	O60669.
KO	K08184.
OMA	GQYKYMY.
OrthoDB	EOG4HX516.
PhylomeDB	O60669.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_20633. Bile salt and organic anion SLC transporters.
SABIO-RK	O60669.
Gene expression databases
ArrayExpress	O60669.
Bgee	O60669.
CleanEx	HS_SLC16A7.
Genevestigator	O60669.
GermOnline	ENSG00000118596. Homo sapiens.
Family and domain databases
InterPro	IPR027178. MCT2. IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR004743. Monocarb_transpt. [Graphical view]
PANTHER	PTHR11360:SF25. PTHR11360:SF25. 1 hit.
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00892. 2A0113. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00119. Pyruvic acid.
GenomeRNAi	9194.
NextBio	34471.
SOURCE	Search...

Entry information

Entry name

MOT2_HUMAN

Accession

Primary (citable) accession number: O60669
Secondary accession number(s): Q8NEM3, Q9UPB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 30, 2010
Last modified:	April 3, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.