O60664 (PLIN3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Perilipin-3

Alternative name(s):
47 kDa mannose 6-phosphate receptor-binding protein
Short name=47 kDa MPR-binding protein
Cargo selection protein TIP47
Mannose-6-phosphate receptor-binding protein 1
Placental protein 17
Short name=PP17

Gene names

Name:	PLIN3
Synonyms:	M6PRBP1, TIP47

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network. Ref.1
Subunit structure	Homooligomer. Interacts with M6PR (via the cytoplasmic domain). Interacts with IGF2R (via the cytoplasmic domain). Isoform 2 may exist as a homodimer (known as PP17C). Ref.1
Subcellular location	Cytoplasm. Endosome membrane; Peripheral membrane protein; Cytoplasmic side Potential. Lipid droplet Potential. Note: Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails. Ref.1 Ref.10
Sequence similarities	Belongs to the perilipin family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm Endosome Lipid droplet Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	vesicle-mediated transport Traceable author statement Ref.1. Source: ProtInc
Cellular_component	Golgi apparatus Traceable author statement Ref.1. Source: ProtInc endosome Traceable author statement Ref.1. Source: ProtInc endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell lipid particle Inferred from direct assay PubMed 14741744. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RHOBTB3	O94955	1	EBI-725795,EBI-2367123

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O60664-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: PP17b.

Isoform 2 (identifier: O60664-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-183: Missing.

Note: PP17a.

Isoform 3 (identifier: O60664-3)

The sequence of this isoform differs from the canonical sequence as follows:
     321-321: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Perilipin-3		PRO_0000099890
Regions
	Coiled coil	252 – 277	26	Potential
	Coiled coil	353 – 377	25	Potential
Amino acid modifications
	Modified residue	65	1	N6-acetyllysine Ref.12
	Modified residue	130	1	Phosphoserine Ref.11 Ref.13
	Modified residue	170	1	Phosphothreonine Ref.11
	Modified residue	175	1	Phosphoserine Ref.11
	Modified residue	179	1	Phosphoserine Ref.11
Natural variations
	Alternative sequence	1 – 183	183	Missing in isoform 2.		VSP_004664
	Alternative sequence	321	1	Missing in isoform 3.		VSP_040325
	Natural variant	56	1	I → V. Ref.1 Ref.2 Ref.3 Ref.8 Corresponds to variant rs8289 [ dbSNP | Ensembl ].		VAR_022780
	Natural variant	275	1	V → A. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5 Ref.6 Ref.8 Corresponds to variant rs9973235 [ dbSNP | Ensembl ].		VAR_024559
Experimental info
	Sequence conflict	77	1	G → W in AAD11622. Ref.2
	Sequence conflict	109 – 111	3	ILQ → MLR in AAD11622. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 11, 2011. Version 3. Checksum: 67B2B9CDBC523043	FASTA	434	47,075
	10 20 30 40 50 60 MSADGAEADG STQVTVEEPV QQPSVVDRVA SMPLISSTCD MVSAAYASTK ESYPHIKTVC 70 80 90 100 110 120 DAAEKGVRTL TAAAVSGAQP ILSKLEPQIA SASEYAHRGL DKLEENLPIL QQPTEKVLAD 130 140 150 160 170 180 TKELVSSKVS GAQEMVSSAK DTVATQLSEA VDATRGAVQS GVDKTKSVVT GGVQSVMGSR 190 200 210 220 230 240 LGQMVLSGVD TVLGKSEEWA DNHLPLTDAE LARIATSLDG FDVASVQQQR QEQSYFVRLG 250 260 270 280 290 300 SLSERLRQHA YEHSLGKLRA TKQRAQEALL QLSQVLSLME TVKQGVDQKL VEGQEKLHQM 310 320 330 340 350 360 WLSWNQKQLQ GPEKEPPKPE QVESRALTMF RDIAQQLQAT CTSLGSSIQG LPTNVKDQVQ 370 380 390 400 410 420 QARRQVEDLQ ATFSSIHSFQ DLSSSILAQS RERVASAREA LDHMVEYVAQ NTPVTWLVGP 430 FAPGITEKAP EEKK
	Isoform 2 [UniParc]. Checksum: 3C7AEC3FBE9429DF	FASTA	251	28,158
	10 20 30 40 50 60 MVLSGVDTVL GKSEEWADNH LPLTDAELAR IATSLDGFDV ASVQQQRQEQ SYFVRLGSLS 70 80 90 100 110 120 ERLRQHAYEH SLGKLRATKQ RAQEALLQLS QVLSLMETVK QGVDQKLVEG QEKLHQMWLS 130 140 150 160 170 180 WNQKQLQGPE KEPPKPEQVE SRALTMFRDI AQQLQATCTS LGSSIQGLPT NVKDQVQQAR 190 200 210 220 230 240 RQVEDLQATF SSIHSFQDLS SSILAQSRER VASAREALDH MVEYVAQNTP VTWLVGPFAP 250 GITEKAPEEK K
	Isoform 3 [UniParc]. Checksum: 8716B72B11C7FE65	FASTA	433	46,947
	10 20 30 40 50 60 MSADGAEADG STQVTVEEPV QQPSVVDRVA SMPLISSTCD MVSAAYASTK ESYPHIKTVC 70 80 90 100 110 120 DAAEKGVRTL TAAAVSGAQP ILSKLEPQIA SASEYAHRGL DKLEENLPIL QQPTEKVLAD 130 140 150 160 170 180 TKELVSSKVS GAQEMVSSAK DTVATQLSEA VDATRGAVQS GVDKTKSVVT GGVQSVMGSR 190 200 210 220 230 240 LGQMVLSGVD TVLGKSEEWA DNHLPLTDAE LARIATSLDG FDVASVQQQR QEQSYFVRLG 250 260 270 280 290 300 SLSERLRQHA YEHSLGKLRA TKQRAQEALL QLSQVLSLME TVKQGVDQKL VEGQEKLHQM 310 320 330 340 350 360 WLSWNQKQLQ GPEKEPPKPE VESRALTMFR DIAQQLQATC TSLGSSIQGL PTNVKDQVQQ 370 380 390 400 410 420 ARRQVEDLQA TFSSIHSFQD LSSSILAQSR ERVASAREAL DHMVEYVAQN TPVTWLVGPF 430 APGITEKAPE EKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TIP47: a cargo selection device for mannose 6-phosphate receptor trafficking." Diaz E., Pfeffer S.R. Cell 93:433-443(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS VAL-56 AND ALA-275, FUNCTION, INTERACTION WITH M6PR AND IGF2R, HOMOOLIGOMERIZATION, SUBCELLULAR LOCATION.
[2]	"Cloning and sequence analysis of cDNAs encoding human placental tissue protein 17 (PP17) variants." Than N.G., Sumegi B., Than G.N., Kispal G., Bohn H. Eur. J. Biochem. 258:752-757(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANTS VAL-56 AND ALA-275. Tissue: Placenta.
[3]	"Cloning and sequencing of human oncodevelopmental soluble placental tissue protein 17 (PP17): homology with adipophilin and the mouse adipose differentiation-related protein." Than N.G., Sumegi B., Than G.N., Kispal G., Bohn H. Tumor Biol. 20:184-192(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANTS VAL-56 AND ALA-275. Tissue: Placenta.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-275.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-275.
[6]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT ALA-275.
[7]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS VAL-56 AND ALA-275. Tissue: Colon and Muscle.
[9]	"Purification and characterization of two new soluble placental tissue proteins (PP13 and PP17)." Bohn H., Kraus W., Winckler W. Oncodev. Biol. Med. 4:343-350(1983) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Tissue: Placenta.
[10]	"Spatial integration of TIP47 and adipophilin in macrophage lipid bodies." Robenek H., Lorkowski S., Schnoor M., Troyer D. J. Biol. Chem. 280:5789-5794(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130; THR-170; SER-175 AND SER-179, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-65, MASS SPECTROMETRY.
[13]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF057140 mRNA. Translation: AAC39751.1. AF055574 mRNA. Translation: AAD11622.1. AF051314 mRNA. Translation: AAD11619.1. AF051315 mRNA. Translation: AAD11620.1. BT007235 mRNA. Translation: AAP35899.1. AK291104 mRNA. Translation: BAF83793.1. AK223054 mRNA. Translation: BAD96774.1. AC027319 Genomic DNA. No translation available. BC001590 mRNA. Translation: AAH01590.1. BC005818 mRNA. Translation: AAH05818.1. BC007566 mRNA. Translation: AAH07566.1. BC019278 mRNA. Translation: AAH19278.1.
IPI	IPI00106668. IPI00303882. IPI00943550.
RefSeq	NP_001157661.1. NM_001164189.1. NP_005808.3. NM_005817.4.
UniGene	Hs.140452.
3D structure databases
ProteinModelPortal	O60664.
SMR	O60664. Positions 202-427.
ModBase	Search...
Protein-protein interaction databases
IntAct	O60664. 7 interactions.
MINT	MINT-5000803.
STRING	9606.ENSP00000221957.
PTM databases
PhosphoSite	O60664.
2D gel databases
REPRODUCTION-2DPAGE	IPI00303882.
Proteomic databases
PaxDb	O60664.
PRIDE	O60664.
Protocols and materials databases
DNASU	10226.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000221957; ENSP00000221957; ENSG00000105355. ENST00000585479; ENSP00000465596; ENSG00000105355.
GeneID	10226.
KEGG	hsa:10226.
UCSC	uc002mbj.2. human. uc002mbl.3. human.
Organism-specific databases
CTD	10226.
GeneCards	GC19M004839.
H-InvDB	HIX0014673.
HGNC	HGNC:16893. PLIN3.
HPA	HPA006427.
MIM	602702. gene.
neXtProt	NX_O60664.
PharmGKB	PA165394001.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG81115.
HOGENOM	HOG000033816.
HOVERGEN	HBG002935.
InParanoid	O60664.
OMA	FAPGITE.
OrthoDB	EOG41ZFB4.
PhylomeDB	O60664.
Gene expression databases
Bgee	O60664.
CleanEx	HS_M6PRBP1.
Genevestigator	O60664.
GermOnline	ENSG00000105355. Homo sapiens.
Family and domain databases
InterPro	IPR004279. Perilipin. [Graphical view]
PANTHER	PTHR14024. PTHR14024. 1 hit.
Pfam	PF03036. Perilipin. 1 hit. [Graphical view]
PIRSF	PIRSF036881. PAT. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	PLIN3. human.
DrugBank	DB01279. Galsulfase. DB01271. Idursulfase.
GenomeRNAi	10226.
NextBio	38722.
PMAP-CutDB	O60664.
SOURCE	Search...

Entry information

Entry name

PLIN3_HUMAN

Accession

Primary (citable) accession number: O60664
Secondary accession number(s): A8K4Y9 , Q53G77, Q9BS03, Q9UBD7, Q9UP92

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 11, 2011
Last modified:	May 1, 2013
This is version 124 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.