O60658 (PDE8A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
EC=3.1.4.17

Gene names

Name:

PDE8A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	829 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development. Ref.11
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions. Ref.11
Enzyme regulation	Inhibited by dipyridimole. Insensitive to selective PDE inhibitors including rolipram and zaprinast as well as to the non-selective inhibitor, IBMX. Unaffected by cGMP.
Pathway	Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Tissue specificity	Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon.
Domain	Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain.
Sequence caution	The sequence AAL18612.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. The sequence AAL18613.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. The sequence AAL18614.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS. The sequence BAG54458.1 differs from that shown. Reason: Intron retention. The sequence EAX01967.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding cAMP
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cyclic nucleotide metabolic process Non-traceable author statement Ref.6. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Traceable author statement. Source: Reactome
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O60658-1)

Also known as: PDE8A1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O60658-2)

Also known as: PDE8A2;

The sequence of this isoform differs from the canonical sequence as follows:
     239-284: Missing.

Isoform 3 (identifier: O60658-3)

Also known as: PDE8A3;

The sequence of this isoform differs from the canonical sequence as follows:
     213-231: ACNSVFTALENSEDAIEIT → SGKEFTMQKRKTEIIYNKM
     232-829: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Isoform 4 (identifier: O60658-4)

Also known as: PDE8A4;

The sequence of this isoform differs from the canonical sequence as follows:
     212-228: RACNSVFTALENSEDAI → SMQILHLKQQWAISQVN
     229-829: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Isoform 5 (identifier: O60658-5)

Also known as: PDE8A5;

The sequence of this isoform differs from the canonical sequence as follows:
     239-272: YANPAFETTMGYQSGELIGKELGEVPINEKKADL → PCCSSSWFGAAHIPSSAPVEVGVGLLPSWSLRRT
     273-829: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 829

829

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A

PRO_0000198838

Regions

Domain

213 – 283

71

PAS

Domain

287 – 329

43

PAC

Region

531 – 813

283

Catalytic By similarity

Sites

Active site

556

1

Proton donor By similarity

Metal binding

560

1

Divalent metal cation 1

Metal binding

596

1

Divalent metal cation 1

Metal binding

597

1

Divalent metal cation 1

Metal binding

597

1

Divalent metal cation 2

Metal binding

726

1

Divalent metal cation 1

Amino acid modifications

Modified residue

20

1

Phosphoserine Ref.9

Modified residue

386

1

Phosphoserine Ref.10

Modified residue

407

1

Phosphoserine Ref.9

Modified residue

457

1

Phosphoserine Ref.9 Ref.10

Natural variations

Alternative sequence

212 – 228

17

RACNS…SEDAI → SMQILHLKQQWAISQVN in isoform 4.

VSP_041674

Alternative sequence

213 – 231

19

ACNSV…AIEIT → SGKEFTMQKRKTEIIYNKM in isoform 3.

VSP_041675

Alternative sequence

229 – 829

601

Missing in isoform 4.

VSP_041676

Alternative sequence

232 – 829

598

Missing in isoform 3.

VSP_041677

Alternative sequence

239 – 284

46

Missing in isoform 2.

VSP_004597

Alternative sequence

239 – 272

34

YANPA…KKADL → PCCSSSWFGAAHIPSSAPVE VGVGLLPSWSLRRT in isoform 5.

VSP_041678

Alternative sequence

273 – 829

557

Missing in isoform 5.

VSP_041679

Experimental info

Mutagenesis

748

1

Y → F: Increases sensitivity to several nonselective or family selective PDE inhibitors. Ref.11

Sequence conflict

55

1

L → V in AAK57641. Ref.2

Sequence conflict

112

1

E → G in AAH60762. Ref.5

Sequence conflict

344

1

H → R in AAK57641. Ref.2

Sequence conflict

344

1

H → R in AAC39763. Ref.6

Sequence conflict

399

1

I → V in AAK57641. Ref.2

Sequence conflict

399

1

I → V in AAC39763. Ref.6

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

829

Helix Strand Turn

Details...

Helix

484 – 491

8

Helix

492 – 494

3

Helix

502 – 508

7

Turn

509 – 511

3

Helix

513 – 524

12

Helix

527 – 530

4

Helix

535 – 547

13

Beta strand

553 – 557

5

Helix

558 – 572

15

Helix

575 – 577

3

Turn

578 – 580

3

Helix

583 – 595

13

Turn

596 – 599

4

Helix

605 – 610

6

Helix

614 – 618

5

Turn

619 – 621

3

Helix

624 – 635

12

Beta strand

638 – 642

5

Turn

645 – 648

4

Helix

651 – 666

16

Helix

670 – 672

3

Beta strand

673 – 675

3

Turn

679 – 681

3

Helix

688 – 690

3

Helix

691 – 696

6

Helix

711 – 726

16

Helix

729 – 731

3

Helix

734 – 757

24

Turn

769 – 771

3

Helix

774 – 784

11

Helix

786 – 796

11

Helix

801 – 807

7

Turn

808 – 811

4

Helix

814 – 817

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (PDE8A1) [UniParc]. Last modified September 19, 2002. Version 2. Checksum: 99BD05EA185A42CD	FASTA	829	93,304
	10 20 30 40 50 60 MGCAPSIHIS ERLVAEDAPS PAAPPLSSGG PRLPQGQKTA ALPRTRGAGL LESELRDGSG 70 80 90 100 110 120 KKVAVADVQF GPMRFHQDQL QVLLVFTKED NQCNGFCRAC EKAGFKCTVT KEAQAVLACF 130 140 150 160 170 180 LDKHHDIIII DHRNPRQLDA EALCRSIRSS KLSENTVIVG VVRRVDREEL SVMPFISAGF 190 200 210 220 230 240 TRRYVENPNI MACYNELLQL EFGEVRSQLK LRACNSVFTA LENSEDAIEI TSEDRFIQYA 250 260 270 280 290 300 NPAFETTMGY QSGELIGKEL GEVPINEKKA DLLDTINSCI RIGKEWQGIY YAKKKNGDNI 310 320 330 340 350 360 QQNVKIIPVI GQGGKIRHYV SIIRVCNGNN KAEKISECVQ SDTHTDNQTG KHKDRRKGSL 370 380 390 400 410 420 DVKAVASRAT EVSSQRRHSS MARIHSMTIE APITKVINII NAAQESSPMP VTEALDRVLE 430 440 450 460 470 480 ILRTTELYSP QFGAKDDDPH ANDLVGGLMS DGLRRLSGNE YVLSTKNTQM VSSNIITPIS 490 500 510 520 530 540 LDDVPPRIAR AMENEEYWDF DIFELEAATH NRPLIYLGLK MFARFGICEF LHCSESTLRS 550 560 570 580 590 600 WLQIIEANYH SSNPYHNSTH SADVLHATAY FLSKERIKET LDPIDEVAAL IAATIHDVDH 610 620 630 640 650 660 PGRTNSFLCN AGSELAILYN DTAVLESHHA ALAFQLTTGD DKCNIFKNME RNDYRTLRQG 670 680 690 700 710 720 IIDMVLATEM TKHFEHVNKF VNSINKPLAT LEENGETDKN QEVINTMLRT PENRTLIKRM 730 740 750 760 770 780 LIKCADVSNP CRPLQYCIEW AARISEEYFS QTDEEKQQGL PVVMPVFDRN TCSIPKSQIS 790 800 810 820 FIDYFITDMF DAWDAFVDLP DLMQHLDNNF KYWKGLDEMK LRNLRPPPE
	Isoform 2 (PDE8A2) [UniParc]. Checksum: 67BB1FBE670D7EFE	FASTA	783	88,293
	10 20 30 40 50 60 MGCAPSIHIS ERLVAEDAPS PAAPPLSSGG PRLPQGQKTA ALPRTRGAGL LESELRDGSG 70 80 90 100 110 120 KKVAVADVQF GPMRFHQDQL QVLLVFTKED NQCNGFCRAC EKAGFKCTVT KEAQAVLACF 130 140 150 160 170 180 LDKHHDIIII DHRNPRQLDA EALCRSIRSS KLSENTVIVG VVRRVDREEL SVMPFISAGF 190 200 210 220 230 240 TRRYVENPNI MACYNELLQL EFGEVRSQLK LRACNSVFTA LENSEDAIEI TSEDRFIQEW 250 260 270 280 290 300 QGIYYAKKKN GDNIQQNVKI IPVIGQGGKI RHYVSIIRVC NGNNKAEKIS ECVQSDTHTD 310 320 330 340 350 360 NQTGKHKDRR KGSLDVKAVA SRATEVSSQR RHSSMARIHS MTIEAPITKV INIINAAQES 370 380 390 400 410 420 SPMPVTEALD RVLEILRTTE LYSPQFGAKD DDPHANDLVG GLMSDGLRRL SGNEYVLSTK 430 440 450 460 470 480 NTQMVSSNII TPISLDDVPP RIARAMENEE YWDFDIFELE AATHNRPLIY LGLKMFARFG 490 500 510 520 530 540 ICEFLHCSES TLRSWLQIIE ANYHSSNPYH NSTHSADVLH ATAYFLSKER IKETLDPIDE 550 560 570 580 590 600 VAALIAATIH DVDHPGRTNS FLCNAGSELA ILYNDTAVLE SHHAALAFQL TTGDDKCNIF 610 620 630 640 650 660 KNMERNDYRT LRQGIIDMVL ATEMTKHFEH VNKFVNSINK PLATLEENGE TDKNQEVINT 670 680 690 700 710 720 MLRTPENRTL IKRMLIKCAD VSNPCRPLQY CIEWAARISE EYFSQTDEEK QQGLPVVMPV 730 740 750 760 770 780 FDRNTCSIPK SQISFIDYFI TDMFDAWDAF VDLPDLMQHL DNNFKYWKGL DEMKLRNLRP PPE
	Isoform 3 (PDE8A3) [UniParc]. Checksum: D7E21A8D19D661A6	FASTA	231	25,744
	10 20 30 40 50 60 MGCAPSIHIS ERLVAEDAPS PAAPPLSSGG PRLPQGQKTA ALPRTRGAGL LESELRDGSG 70 80 90 100 110 120 KKVAVADVQF GPMRFHQDQL QVLLVFTKED NQCNGFCRAC EKAGFKCTVT KEAQAVLACF 130 140 150 160 170 180 LDKHHDIIII DHRNPRQLDA EALCRSIRSS KLSENTVIVG VVRRVDREEL SVMPFISAGF 190 200 210 220 230 TRRYVENPNI MACYNELLQL EFGEVRSQLK LRSGKEFTMQ KRKTEIIYNK M
	Isoform 4 (PDE8A4) [UniParc]. Checksum: 4667A08AAA57F17F	FASTA	228	25,279
	10 20 30 40 50 60 MGCAPSIHIS ERLVAEDAPS PAAPPLSSGG PRLPQGQKTA ALPRTRGAGL LESELRDGSG 70 80 90 100 110 120 KKVAVADVQF GPMRFHQDQL QVLLVFTKED NQCNGFCRAC EKAGFKCTVT KEAQAVLACF 130 140 150 160 170 180 LDKHHDIIII DHRNPRQLDA EALCRSIRSS KLSENTVIVG VVRRVDREEL SVMPFISAGF 190 200 210 220 TRRYVENPNI MACYNELLQL EFGEVRSQLK LSMQILHLKQ QWAISQVN
	Isoform 5 (PDE8A5) [UniParc]. Checksum: FEECE2A0C9115E13	FASTA	272	29,852
	10 20 30 40 50 60 MGCAPSIHIS ERLVAEDAPS PAAPPLSSGG PRLPQGQKTA ALPRTRGAGL LESELRDGSG 70 80 90 100 110 120 KKVAVADVQF GPMRFHQDQL QVLLVFTKED NQCNGFCRAC EKAGFKCTVT KEAQAVLACF 130 140 150 160 170 180 LDKHHDIIII DHRNPRQLDA EALCRSIRSS KLSENTVIVG VVRRVDREEL SVMPFISAGF 190 200 210 220 230 240 TRRYVENPNI MACYNELLQL EFGEVRSQLK LRACNSVFTA LENSEDAIEI TSEDRFIQPC 250 260 270 CSSSWFGAAH IPSSAPVEVG VGLLPSWSLR RT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human phosphodiesterase 8A splice variants: cloning, gene organization, and tissue distribution." Wang P., Wu P., Egan R.W., Billah M.M. Gene 280:183-194(2001) [PubMed: 11738832] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4 AND 5). Tissue: Testis.
[2]	"T cell activation up-regulates cyclic nucleotide phosphodiesterases 8A1 and 7A3." Glavas N.A., Ostenson C., Schaefer J.B., Vasta V., Beavo J.A. Proc. Natl. Acad. Sci. U.S.A. 98:6319-6324(2001) [PubMed: 11371644] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed: 16572171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Liver and Placenta.
[6]	"Isolation and characterization of PDE8A, a novel human cAMP-specific phosphodiesterase." Fisher D.A., Smith J.F., Pillar J.S., St Denis S.H., Cheng J.B. Biochem. Biophys. Res. Commun. 246:570-577(1998) [PubMed: 9618252] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 117-829 (ISOFORMS 1 AND 2).
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 381-829 (ISOFORM 1). Tissue: Hippocampus.
[8]	The European IMAGE consortium Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 554-829.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20; SER-407 AND SER-457, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-386 AND SER-457, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Kinetic and structural studies of phosphodiesterase-8A and implication on the inhibitor selectivity." Wang H., Yan Z., Yang S., Cai J., Robinson H., Ke H. Biochemistry 47:12760-12768(2008) [PubMed: 18983167] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 482-819 IN COMPLEX WITH METAL IONS AND THE INHIBITOR IBMX, FUNCTION, COFACTOR, MUTAGENESIS OF TYR-748.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF388183 mRNA. Translation: AAL18610.1.
AF388184 mRNA. Translation: AAL18611.1.
AF388185 mRNA. Translation: AAL18612.1. Sequence problems.
AF388186 mRNA. Translation: AAL18613.1. Sequence problems.
AF388187 mRNA. Translation: AAL18614.1. Sequence problems.
AF332653 mRNA. Translation: AAK57641.1.
AC027078 Genomic DNA. No translation available.
AC087468 Genomic DNA. No translation available.
CH471101 Genomic DNA. Translation: EAX01967.1. Sequence problems.
BC060762 mRNA. Translation: AAH60762.1.
BC075822 mRNA. Translation: AAH75822.1.
AF056490 mRNA. Translation: AAC39763.1.
AK127232 mRNA. Translation: BAG54458.1. Sequence problems.
AL109687 mRNA. Translation: CAB52020.1.
AL109778 mRNA. Translation: CAB52432.1.

IPI

IPI00030976.
IPI00182447.
IPI00218161.
IPI00218162.
IPI01025651.

PIR

JW0088.

RefSeq

NP_001230066.1. NM_001243137.1.
NP_002596.1. NM_002605.2.
NP_775656.1. NM_173454.1.

UniGene

Hs.9333.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LHQ	model	-	A	482-821	[»]
1LXX	model	-	A	488-821	[»]
3ECM	X-ray	1.90	A	482-819	[»]
3ECN	X-ray	2.10	A/B	482-819	[»]

ProteinModelPortal

O60658.

SMR

O60658. Positions 80-201, 227-333, 482-819.

ModBase

Search...

Protein-protein interaction databases

IntAct

O60658. 2 interactions.

STRING

O60658.

PTM databases

PhosphoSite

O60658.

Proteomic databases

PRIDE

O60658.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000310298; ENSP00000311453; ENSG00000073417.
ENST00000394553; ENSP00000378056; ENSG00000073417.

GeneID

5151.

KEGG

hsa:5151.

UCSC

uc002blh.1. human.
uc002bli.1. human.

Organism-specific databases

CTD

5151.

GeneCards

GC15P085523.

H-InvDB

HIX0012539.

HGNC

HGNC:8793. PDE8A.

HPA

HPA007722.

MIM

602972. gene.

neXtProt

NX_O60658.

PharmGKB

PA33141.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG18615.

HOVERGEN

HBG053544.

InParanoid

O60658.

OMA

VLACFLD.

OrthoDB

EOG4SBDX7.

PhylomeDB

O60658.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

ArrayExpress

O60658.

Bgee

O60658.

CleanEx

HS_PDE8A.

Genevestigator

O60658.

GermOnline

ENSG00000073417. Homo sapiens.

Family and domain databases

InterPro

IPR003607. Metal-dep_PHydrolase_HD_dom.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]

Gene3D

G3DSA:1.10.1300.10. PDEase_catalytic_dom. 1 hit.

KO

K01120.

Pfam

PF00989. PAS. 1 hit.
PF00233. PDEase_I. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]

PRINTS

PR00387. PDIESTERASE1.

SMART

SM00471. HDc. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00229. Sensory_box. 1 hit.

PROSITE

PS50113. PAC. False negative.
PS50112. PAS. 1 hit.
PS00126. PDEASE_I. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

19878.

SOURCE

Search...

Entry information

Entry name

PDE8A_HUMAN

Accession

Primary (citable) accession number: O60658
Secondary accession number(s): B3KXE6 , Q6P9H3, Q969I1, Q96PC9, Q96PD0, Q96PD1, Q96T71, Q9UMB7, Q9UMC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	September 19, 2002
Last modified:	January 25, 2012
This is version 125 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.